Neurochem Res. 2005 Feb;30(2):201-5. Related Articles, Links
Histological co-localization of iron in Abeta plaques of PS/APP
transgenic mice.
Falangola MF, Lee SP, Nixon RA, Duff K, Helpern JA.
Center for Advanced Brain Imaging, Nathan Kline Institute for
Psychiatric Research, 140 Old Orangeburg Road, Orangeburg, NY 10962,
USA. falangola@nki.rfmh.org
This study confirms the presence of iron, co-localized with Abeta
plaques, in PS/APP mouse brain, using Perls' stain for Fe3+
supplemented by 3,3'-diaminobenzidine (DAB) and Abeta
immunohistochemistry in histological brains sections fixed with
formalin or methacarn. In this study, the fixation process and the
slice thickness did not interfere with the Perls' technique. The
presence of iron in beta-amyloid plaques in PS/APP transgenic mice, a
model of Alzheimer's disease (AD) pathology, may explain previous
reports of reductions of transverse relaxation time (T2) in MRI studies
and represent the source of the intrinsic Abeta plaque MR contrast in
this model.
PMID: 15895823 [PubMed - indexed for MEDLINE]
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