PhD Candidate Trying to Make an Immunoconjugate

Thread view:

Enable EMail Alerts

Start New Thread

Thread rating:

Protoman - 18 Aug 2006 07:32 GMT

Hey all, I'm a immunology PhD candidate, and I'm testing a cancer
therapy in mice for my dissertation. How would I make a
immunoconjugate's payload be part of the antibody itself, instead of
being merely "glued" on? The reason is b/c I'm using sodium
fluoroacetate as the payload, and I don't want it to fall off the
antibody, wreaking all sorts of catastrophic effects. Poor mousey... I
think I have to use genetic engineering, but after that, I'm lost. Any
help here? Thanks!!!!!

PS If you tell me how, I'll list you in the credits section!
PPS Is this the right group to be posting in? If not, please tell me
where a better place would be.

Reply to this Message

Bob - 19 Aug 2006 03:18 GMT

>Hey all, I'm a immunology PhD candidate, and I'm testing a cancer
>therapy in mice for my dissertation. How would I make a
>immunoconjugate's payload be part of the antibody itself, instead of
>being merely "glued" on?

What do you mean "glued on"??

How you attach it depends on what it is. The goal is commonly to
attach it in such a way that it gets where you want it, and then comes
off. Read the literature! Thoroughly.

You will probably need to sit down with an organic chemist to work out
specifics. But that plan must take into account both the biology you
have in mind as well as practical chemistry. It can be a fair amount
of work to figure out a way that works.

>PPS Is this the right group to be posting in? If not, please tell me
>where a better place would be.

How about sci.bio.technology ??

bob

Reply to this Message

Protoman - 19 Aug 2006 06:31 GMT

> >Hey all, I'm a immunology PhD candidate, and I'm testing a cancer
> >therapy in mice for my dissertation. How would I make a
[quoted text clipped - 18 lines]
>
> bob

I mean chemically bonded when I say "glued on". Thanks!!!!

Reply to this Message