J Clin Microbiol. 2006 May;44(5):1650-8. Links

Nutritional requirements and antibiotic resistance patterns of
helicobacter species in chemically defined media.

Testerman TL, Conn PB, Mobley HL, McGee DJ.

Department of Microbiology and Immunology, Louisiana State University
Health Sciences Center-Shreveport, Room 2-221, Shreveport, LA 71130.
tteste@lsuhsc.edu.

The growth of the gastric pathogen Helicobacter pylori in the absence
of serum remains challenging, and nutritional requirements have only
partially been defined, while almost nothing is known about nutritional
requirements of other Helicobacter spp. Although previous data showed
that H. pylori grows in the chemically defined medium F-12, but not in
other tissue culture media examined, the specific components
responsible for growth were not entirely understood. Here we describe
the optimization of amino acids, metals, and sodium chloride for H.
pylori. Iron, zinc, and magnesium were critical for growth; copper was
not required. Optimization of sodium chloride was further beneficial.
Nutritional requirements and antibiotic resistance patterns of several
other Helicobacter spp. revealed that all except H. felis grew in
serum-free, unsupplemented F-12. All Helicobacter spp. were resistant
to at least six antimicrobial agents when cultured in the presence of
serum. However, in the absence of serum, H. pylori, H. mustelae, and H.
muridarum became sensitive to polymyxin B and/or trimethoprim. Much of
the data were obtained using a convenient ATP assay to quantify growth.
H. pylori has surprisingly few absolute requirements for growth: 9
amino acids, sodium and potassium chloride, thiamine, iron, zinc,
magnesium, hypoxanthine, and pyruvate. These data suggest that H.
pylori and other Helicobacter spp. are not as fastidious as previously
thought. The data also suggest that chemically defined media described
herein could yield the growth of a wide range of Helicobacter spp.,
allowing a more detailed characterization of Helicobacter physiology
and interactions with host cells.

PMID: 16672389 [PubMed - in process]

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