Biochem Pharmacol. 2005 Feb 1;69(3):395-406. Epub 2004 Dec 15. Related
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Dietary rutin, but not its aglycone quercetin, ameliorates dextran
sulfate sodium-induced experimental colitis in mice: attenuation of
pro-inflammatory gene expression.

Kwon KH, Murakami A, Tanaka T, Ohigashi H.

Division of Food Science and Biotechnology, Graduate School of
Agriculture, Kyoto University, Kyoto 606-8502, Japan.

Oxidative stress has been shown to play a pivotal role in the onset of
inflammatory bowel disease (IBD) and carcinogenesis. We evaluated the
effects of two dietary anti-oxidants, rutin and its aglycone quercetin,
on dextran sulfate sodium (DSS)-induced experimental colitis in mice.
Female ICR mice were fed a diet containing 0.1% rutin or 0.1% quercetin
for 2 weeks, and given 5% DSS in drinking water during the second week
to induce colitis. We also examined the dose-dependency of rutin and
quercetin (0.01% and 0.001% each) as well as their therapeutic
efficacy, which was evaluated following DSS administration, on
DSS-induced colitis. The protein level of interleukin (IL)-1beta in
both colonic mucosa and peritoneal macrophages was quantified by
enzyme-linked immunosorbent assay. Further, mRNA expression levels of
IL-1beta, tumor necrosis factor-alpha, IL-6, granulocyte
macrophage-colony stimulating factor, inducible nitric oxide synthase,
and cyclooxygenase (COX)-1 and COX-2 in colonic mucosa were determined
by reverse transcription-polymerase chain reaction. A diet containing
0.1% rutin, but not quercetin, attenuated DSS-induced body weight loss
and shortening of the colorectum (P<0.01 and <0.05, respectively), and
dramatically improved colitis histological scores. Further, DSS-induced
increases in colonic mucosal IL-1beta levels were blunted significantly
in rutin-, but not quercetin-, fed mice (P<0.01), while dietary rutin
attenuated the expressions of IL-1beta and IL-6 mRNA in colonic mucosa
(each, P<0.01). As for dose dependency, 0.01%, but not 0.001%, dietary
rutin significantly reduced mucosal IL-1beta levels (P<0.01). Notably,
a 0.1% rutin diet given 3 days after DSS treatment significantly
suppressed both colorectal shortening and IL-1beta production (P<0.05
and <0.01, respectively). Dietary rutin ameliorates DSS-induced
colitis, presumably by suppressing the induction of pro-inflammatory
cytokines. Our results suggest that rutin may be useful for the
prevention and treatment of IBD and colorectal carcinogenesis via
attenuation of pro-inflammatory cytokine production.

PMID: 15652231 [PubMed - as supplied by publisher]

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