This would give some credence to the theory of targeting of iron as an
antiinflammatory.
Since rutin is a potent iron binder.

<<snip>>
The present study revealed that rutin possesses anti-inflammatory properties.
<<snip>>

Rev Med Chir Soc Med Nat Iasi. 2002 Oct-Dec;106(4):801-5.  Related Articles,
Links  

[In Process Citation]

[Article in Romanian]

Gherase F, Pavelescu MD, Stanescu U, Grigorescu E.

Facultatea de Farmacie, Disciplina de Farmacodinamie, Universitatea de Medicina
si Farmacie Gr.T. Popa Iasi.

In the present investigation we have evaluated the potential existence of
analgesic activity of the residue of tincture and of the crude lipophilic (AC
2, AC 4) and hydrophilic portions (AC 1, AC 3) of infusion and decoction
obtained from flower heads of Achillea collina J. Becker ex Reichenb. Acid
acetic induced writhing and vascular permeability test was performed by the
method of Whittle. Comparison was made with indomethacin, morphine and rutin,
as references drugs. The mice were dosed orally with samples. AC 2 induced a
potent inhibition (47.23%; p < 0.05) of the nociceptive response after oral
administration of 250 mg/kg--a dose ten folds higher than the dose of
indomethacin. AC 3, at a dose of 5000 mg/kg (p.o.) inhibited with 47.6% (p <
0.001) and 19.8% (p < 0.05) the increase in peritoneal vascular permeability
caused by 0.5% acetic acid. Relative unexpected, morphine (1.14 mg/kg, p.o.),
an opioid analgesic, also produced a significant reduction of the leakage
(41.2%, p < 0.01). This finding is consistent with the reports in which the
role of the endogenous opiate system in the physiological response to
injury/inflammation, mainly controlling fluid leakage into the interstitial
space, is sustained.

PMID: 14974233 [PubMed - in process]

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Exp Toxicol Pathol. 2003 Mar;54(4):313-8.  Related Articles, Links  

Anti-inflammatory effect of rutin on rat paw oedema, and on neutrophils
chemotaxis and degranulation.

Selloum L, Bouriche H, Tigrine C, Boudoukha C.

Laboratory of Applied Biochemistry, Department of Biology, Faculty of Sciences,
University of Ferhat ABBAS, 19000 Setif, Algeria. laidsel@yahoo.com

BACKGROUND: Rutin, a natural flavone derivative, is known for its
pharmacological properties. We have previously reported that this flavonol
exerted a potent inhibitory effect on respiratory burst of
fMet-Leu-Phe-stimulated neutrophils, as well as on phosphoinositide 3-kinase
gamma activity in a cell free system. In the present study, the
anti-inflammatory effect of rutin was investigated in vivo and in vitro.
METHODS: rutin or aspirin (100 mg/kg, body weight) were given orally to rats 1
hour before paw oedema induction, using lambda-carrageenan 1%. The rat paw
volume was measured by mean of plethysmometer, initially and during 6 hours.
The chemotaxis of neutrophils towards 10(-7) M fMet-Leu-Phe was performed using
48-well chemotaxis chamber. Neutrophils that migrated through 5 microm pore
size polycarbonate filter, in presence or in absence of rutin, were counted
microscopically. Elastase exocytosis of either phorbol 12-myristate 13-acetate
or fMet-Leu-Phe/cytochalasin B-stimulated neutrophils was assessed in absence
or in presence of rutin using the synthetic substrate
N-Suc-Ala-Ala-Ala-p-nitroanilide. The absorbance of released p-nitroaniline was
measured at 405 nm using microplate reader. RESULTS: The maximal swelling in
placebo group was observed at 5 hours, after lambda-carrageenan injection. Oral
administration of rutin reduced rat paw swelling starting 2 hours after
lambda-carrageenan injection. Rutin reduced significantly (p < 0.05) and in a
dose-dependant manner the polymorphonuclear neutrophils chemotaxis to
fMet-Leu-Phe. Furthermore, elastase exocytosis, induced by both stimuli, was
partially inhibited by rutin up to 25 microM. CONCLUSION: The present study
revealed that rutin possesses anti-inflammatory properties.

PMID: 12710715 [PubMed - indexed for MEDLINE]

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