vitamin E levels acts as a protective factor

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doe - 15 Feb 2004 20:05 GMT

Food Chem Toxicol. 2004 Mar;42(3):459-64. Related Articles, Links

Effect of an acute dose of ethanol on lipid peroxidation in rats: action of
vitamin E.

Jordao AA Jr, Chiarello PG, Arantes MR, Meirelles MS, Vannucchi H.

Postgraduation in Food Science, Faculty of Pharmaceutical Sciences, University
of Sao Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirao Preto-SP, Brazil.

Free radical generation is an important step in the pathogenesis of
ethanol-associated liver injury. Administration of ethanol induces an increase
in lipid peroxidation both by enhancing the production of oxygen reactive
species and by decreasing the levels of endogenous antioxidants. This work
focuses on the generation of free radicals provoked by an acute ethanol dose in
rats, and the role of different dietary levels of vitamin E. The objective of
this investigation was to study the effect of three different dietary levels of
vitamin E (deficient, control and supplemented with 20 times higher levels) on
plasma and liver lipid peroxidation (assayed by TBARS), vitamin E in plasma and
liver, and hepatic glutathione concentration, in rats receiving the different
diets. The animals were submitted to an acute dose of ethanol (5 g/kg body
weight) administered by gavage at the end of an experimental 4 week period and
were sacrificed at 0, 2, 4, 8 and 24 h after ethanol administration. Dietary
vitamin E caused a dose-dependent increase in liver and plasma concentration of
the vitamin, but ethanol administration decreased hepatic vitamin E in all
groups. TBARS concentrations were higher in liver of rats that received the
deficient diet, independent of ethanol, however, liver TBARS concentrations
were low in control and supplemented groups, but increased with ethanol
ingestion. Glutathione levels were lowered by ethanol administration in all
groups, in different times, but recovered to this original level in 24 h time.
In conclusion, vitamin E deficiency alone induces liver lipid peroxidation in
rats, acute administration of ethanol affect vitamin E and GSH level and
maintenance of adequate or higher vitamin E levels acts as a protective factor
against free radical generation.

PMID: 14871588 [PubMed - in process]

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Manky Badger - 15 Feb 2004 21:11 GMT

"doe" <ironjustice@aol.comdoe> wrote in message
news:20040215150512.13026.00002233@mb-m02.aol.com...

> Food Chem Toxicol. 2004 Mar;42(3):459-64. Related Articles, Links
>
> Effect of an acute dose of ethanol on lipid peroxidation in rats: action of
> vitamin E.

Speaking as one who's actually looked into this recently.........

The article I've snipped doesn't go into specific details about the
technique by which vitamin E was measured.
There are a few methods available, most of which are rather time consuming,
and many of which aren't very precise.
Without more details about the reproducubility of the assay employed, I'd be
rather sceptical about the article.

MB

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doe - 16 Feb 2004 19:47 GMT

>Subject: Re: vitamin E levels acts as a protective factor
>From: "Manky Badger" mb@idontwantspamdotpuritancotfreeserve.co.uk
[quoted text clipped - 19 lines]
>
>MB

Arch Biochem Biophys. 2004 Mar 1;423(1):162-9. Related Articles, Links

Enhanced inflammatory responses in alpha-tocopherol transfer protein null mice.

Schock BC, Van Der Vliet A, Corbacho AM, Leonard SW, Finkelstein E, Valacchi G,
Obermueller-Jevic U, Cross CE, Traber MG.

Division of Pulmonary and Critical Care Medicine and Center for Comparative
Respiratory Biology and Medicine, University of California School of Medicine,
Davis, CA 95616, USA.

The liver preferentially secretes alpha-tocopherol into plasma under the
control of the hepatic alpha-tocopherol transfer protein (alpha-TTP).
alpha-TTP-null mice (Ttpa(-/-) mice) are vitamin E deficient, therefore were
used for investigations of in vivo responses to sub-normal tissue
alpha-tocopherol concentrations during inflammation. Increased basal oxidative
stress in Ttpa(-/-) mice was documented by increased plasma lipid peroxidation,
and superoxide production by bone marrow-derived neutrophils stimulated in
vitro with phorbol 12-myristate 13-acetate. Lipopolysaccharide (LPS) injected
intraperitoneally induced increases in lung and liver HO-1 and iNOS, as well as
plasma NO(x) in Ttpa(+/+) mice. LPS induced more modest increases in these
markers in Ttpa(-/-) mice, while more marked increases in plasma IL-10 and lung
lavage TNFalpha were observed. Taken together, these results demonstrate that
alpha-tocopherol is important for proper modulation of inflammatory responses
and that sub-optimal alpha-tocopherol concentrations may derange
inflammatory-immune responses.

PMID: 14871478 [PubMed - in process]

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anon - 16 Feb 2004 02:48 GMT

> Food Chem Toxicol. 2004 Mar;42(3):459-64. Related Articles, Links
> Effect of an acute dose of ethanol on lipid peroxidation in rats: action of
> vitamin E.

Wow! This is great news for alcoholic rats everywhere! ;)

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