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Medical Forum / General / Laboratory / October 2006how can i isolate Rotavirus??
hellooo everybody.. i'm a med. tech. intern,, i'm training now in virology lab i need to know in which cell line can we isolate Rotavirus?? cuz in this lab, they use the latex agglutination method to detect rotavirus infections, it's really worthless, i guess!! cuz i have to repeat the test 3 times with 3 different kits, to make sure that a positive one it's a real positve( i've never heard about such a protocol). all these kits have a variable % of sensitivity, & i really prefer the isolation method!! thanx http://www.remelinc.com/products/clinical/level2/Rotavirus.cfm We use the Remel SAS Rotavirus test. It takes 15 minutes and works very well. Our docs can't wait that long for results. The latex agglutination tests are not as good as the EIA tests. You should really look into those. I haven't done latex testing for Rotavirus in at least 15 years. If you're a MT intern, you're pretty much bound to do things by whatever rules the lab you're working for uses. You can't just go off on your own and decide to grow rotavirus as a methodology. Student MT's are pretty much at the bottom of the pecking order in laboratories. I would think that all the bacteria in stools would contaminate your cell lines with bacterial overgrowth. I can't tell you the last time I've seen an order for viral culture on a stool. Judy Dilworth, M.T. (ASCP) Microbiology > hellooo everybody.. > > i'm a med. tech. intern,, i'm training now in virology lab > > i need to know in which cell line can we isolate Rotavirus?? 15 YEARS!!! Woooo,, that's a really long time! I can believe that we still depend on it in diagnosis. You're right about us " intern students", but here's the good news.. We can with the help from our Dr's in our college do our own small researches during the internship year. It has nothing to do with our bachelor degree project (which actually what we did last year). The main aim is to help the labs that we're training in to improve their work or quality. In the end of every year, we have a students meeting organized by our college where we present our works and projects. The college invites many heads of lab from different hospitals & medical centers in the city to that meeting. & that's what I'm trying to do,, I want to prove to my lab that this latex method it's not useful & they have to find another way. Getting a 1 ml of cell line suspension & then subculture it is much easiest for me than ordering a kit. But I will use your idea to include it in my thesis. Did you mean that I can't culture a stool specimen?? If so,, how can I process such a specimen for viral studies? Thank you for replying I don't work in Virology so I don't know how stools are processed. I shouldn't have spoken out of turn. Any Virology people in clinical labs out there? Please help! I don't know where your lab is located but they really need to review current methodologies. Judy Dilworth, M.T. (ASCP) Microbiology > 15 YEARS!!! > Woooo,, that's a really long time! I can believe that we still depend > on it in diagnosis. |
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