CAP requires that all post vas specimens and those fertility specimens
showing azoospermia or oligozoospermia need to be concentrated.  Our
procedure is to let the semen liquify and then centrifuge like a urine
sample. (5 min at 1500rpm), then examine the button for sperm.

Here you go...

Principle:

To evaluate a seminal fluid for absence of spermatozoa following a
vasectomy

Patient and Specimen Requirements:

     Conditions for Patient Preparation

1.       Patient will follow the physician's orders for the required
time interval between the vasectomy procedure and seminal analysis
testing - usually 2 months.

2.       Patient will collect the specimen by masturbation, not by
coitus interuptus.

3.       Patient will collect the specimen in a clean, dry, sterile
container that is supplied by the lab, or the physician's office.

    Specimen Type

·         Semen sample

Material Requirements and Preparation:

Urinalysis centrifuge tube

Glass slide

Glass coverslip

Procedure:

1.  Transfer specimen to a urinalysis centrifuge tube.

2.  Centrifuge at 2000 rpm for 5 minutes.

3.  Aspirate supernatant with pipette and deposit back into original
container.

4.  Place one drop of sediment onto glass slide.  Coverslip with a 22 x
22 glass coverslip.

5.  Scan entire preparation using x40 objective for spermatozoa.

Reference Intervals and Interpretation:

Choose one of the queries listed as required at each of the result
entry fields.

Sperm:         none seen/present

Quantity:      rare              <5/hpf

                   few                          6-10/hpf

                   moderate   11-20/hpf

                   many            >20/hpf

Motility:        non-motile/motile

References:

Cannon,D.  Examination of Seminal Fluid.
Knight, Kjeldsberg.  Blody Fluids 2nd Edition.
Consultation with SWCHSC Clinical Pathology Department
Adelman, M, Cahill,E.  Atlas of Sperm Morphology.  ASCP. 1989.