> >> Hi. I'm a med tech student and recently "learned" in clin chem that 1:2
> >> means 1 part IN 2. Huh? When I pointed out that most of the universe
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>
> bob
>"Bob" <bbruner@uclink4.berkeley.edu> wrote in message
<snip>
In part, this is a turf-war, and I have been playing into it, too.
So let me try to be neutral for a moment (before pouncing on you
again).
For whatever reason, the dilution designation tends to be used
differently by people in different fields. This in itself is
confusing, and it doesn't really matter why the difference. That is
really the point of the OP, and I applaud him for noticing it. But I
should also note that such terminology differences between fields are
not all that uncommon. Students like to think that science is precise,
and that scientific terminology is precise. Perhaps it is, but it is
also inconsistent between fields. The reason is simple enough.
Historically, terminology in different fields tends to develop
independently. Even people in different areas of chemistry find that
some terms are used differently between them. So it really is
important to be alert. A term means what the person using it intends
it to mean -- as Humpty Dumpty taught us, in more elegant words.
Ok, now back to partisanship and the real truth...
>The term "dilution" does not come from chemistry. It is a serological term.
Huh? That is hard to believe. Chem is a much older science than
serology. I don't have any facts at hand on this particular point, but
I would be quite surprised if the use of dilutions in both chem and
microbiol did not precede use in serology. There might be an
interesting story here, if anyone actually knows some historical
facts.
(The word itself dates from 17th century, but not sure we learn much
from that per se.)
>As you mention in chemistry you only see the ratio symbol when using moles
>or formulas to be converted into working solutions or quantities of
>appropriate volumnes or concentration as there are no standard
>concentrations of stock chemicals. It is inappropriate to state ratios
>without stating concentrations.
Nonsense. One does not have to know anything about concentrations in
order to do dilutions or state ratios. A statement to dilute something
10-fold (by volume -- and that part is generally understood unless
specified otherwise) is fully meaningful, and does not depend in any
way on knowing anything about concentration.
I bet that most dilutions are done on unknowns, rather than on
solutions of known conc. (Also in microbiology.)
> In chemistry you must always
>know what the concentration of the original solution is.
Not true. See previous point.
There is no fundamental difference in how dilutions are used in chem
and in serology. In both cases, the basic point is to get the conc
into a desirable range. If there is a difference in how dilutions are
specified, it is just an historical idiosyncrasy, as discussed above.
bob