ICP34.5 interfere with apoptosis

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Perl Molson - 30 Aug 2004 01:22 GMT

http://darwin.bio.uci.edu/~faculty/wagner/hsv7f.html
This process may be augmented by viral genes shown to interfere with
apoptosis, such as ICP34.5, which act to prevent neuronal death during
reactivation where limited replication occurs.

but the arginine is providing the ICP34.5 protein to grow but this
protein is as follows:
(The ICP34.5 protein of herpes simplex virus type 1 (HSV-1)1 is
important for promoting viral neurovirulence
http://www.jbc.org/cgi/content/full/277/13/11423
)

So now it gets nasty ;O)
The bottom line here:
ICP34.5 is either interefering with apoptosis and it is important to
promote viral neurovirulence; arginine seems to provide ICP34.5
protein to grow.

Once you have it, ICP34.5 you can't get rid of it but it will cause
trouble also. Not good.

I am now wondering how much ICP34.5 will interfere with the apoposis
of the
neuronal cell; is it really that crucial in mantaining apoptosis?
If eitherwise, by targetting ICP34.5 we can eventually
stop the herpes virus from its cycle namely cure from herpes simplex.

Perl Molson

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Tim Fitzmaurice - 06 Sep 2004 17:18 GMT

> So now it gets nasty ;O)

It gets very nasty....

> The bottom line here:
> ICP34.5 is either interefering with apoptosis and it is important to
> promote viral neurovirulence;

HSV1 does try to block apoptosis at certain times....HSV2 seems to do it
much less. Exactly how and when is a big question. However when I was
trying some compounds out on HSV1 using a compound that stopped it
(prostaglandin analogue) there was an antiviral effect...

> arginine seems to provide ICP34.5
> protein to grow.

Relating a pool of amino acid coming into the body is always VERY hard to
relate to that pool then going to the precise cell subcompartment to
alevel to misintegrate....was always the problem with the lysine
story/propsed mechanism and just as much in arginine - despite finding
some data floating about that modifying amounts in or out of one affect
virus replication....note this is very different dealing with the whole
body as opposed say topical as the issue becomes less problematic to
explain for topical.

> If eitherwise, by targetting ICP34.5 we can eventually
> stop the herpes virus from its cycle namely cure from herpes simplex.

Thats one of the ideas.....I think there are some mutants out there where
they delete or modify the virus..see if anyone has published.

Tim
--
When playing rugby, its not the winning that counts, but the taking apart
ICQ: 5178568

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Perl Molson - 08 Sep 2004 11:11 GMT

> > So now it gets nasty ;O)
>
[quoted text clipped - 28 lines]
>
> Tim

I did already, in one of my old posts:
http://www.pahealthsystems.com/message69720.html

Author

Perl Molson

ICP34.5 engineered HSV vaccine not a cure for herpes, eventually
In summary, we have found that deletion of the ICP34.5
open reading frame from McKrae, a highly virulent HSV-1
strain, results in an avirulent virus that is severely restricted for
growth in eyes and TG and probably establishes latency at a
reduced rate. Despite these impairments, this mutant was still
capable of low levels of spontaneous reactivation in the rabbit.
This may be an important consideration with respect to the use
of ICP34.5 deletion mutants as genetically engineered HSV
vaccines.

http://jvi.asm.org/cgi/reprint/69/5..nt&pmid=7707530

http://groups.google.com/groups?dq=&hl=en&lr=&ie=UTF-8&newwindow=1&group=alt.sup
port.herpes&c2coff=1&safe=off&selm=813d1c43.0408291648.2f8644ca%40posting.google
.com

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