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Medical Forum / Diseases and Disorders / Herpes / June 2004

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Proteins Bound by   Prunella vulgaris Polysaccharide

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Perl Molson - 16 Jun 2004 19:22 GMT
Identification of Herpes Simplex Virus 1 Proteins Bound by the Novel
Anti-Herpes Prunella vulgaris Polysaccharide
G. DELANEY1, S. H. S. LEE1*, AND S. F. LEE1,2. 1Dept. of Microbiology
& Immunology, 2Dept. of Applied Oral Sciences, Dalhousie University,
Halifax, Nova Scotia.

Objective: We previously described a polysaccharide from the Chinese
herb Prunella vulgaris exhibiting activity against herpes simplex
virus 1 and 2 (HSV-1 and 2). The polysaccharide apparently inhibited
viral binding as well as events post-viral binding and penetration.
The objective of this study is to identify the viral proteins bound by
the Prunella polysaccharide.

Methods: [35S]methionine-labeled proteins were obtained from
HSV-1-infected Vero cell lysates by freeze-thaw cycles and Noindet
P-40 and sodium deoxycholate treatment. The proteins were applied to a
Prunella polysaccharide-Sepharose column. Bound proteins were eluted
by Prunella polysaccharide, immuno-precipitated by anti-gC and anti-gD
antibodies and analyzed by SDS-PAGE.

Results: Following chromatography on the Prunella polysaccharide
affinity column, two broad bands of 120-112 kDa and 69- 74 kDa were
obtained from the HSV-1 infected lysate; these protein bands were
absent from the mock infected lysate suggesting that the proteins were
of viral in origin. Similar proteins were obtained when
sucrose-gradient purified HSV-1 lysate was used. Two protein bands of
115 kDa and 74 kDa were recovered from the eluted proteins by
immuno-precipitation with the anti-gC antibody, while a 68 kDa protein
band was precipitated by the anti-gD antibody. The 115 kDa and 68 kDa
proteins corresponded to the reported size of gC and gD, respectively.
The nature of the 74 kDa is unclear but it could be a truncated
version of the gC or another HSV-1 protein which shared common
antigenic epitopes with gC.

http://www.cacmid.ca/abstracts/a50.html
Gadge - 16 Jun 2004 23:16 GMT
There's an episode of Frasier that comes to mind here

It's the one where Frasier's dad reckon his dog is way more intelligent than
the average dog and can understand much of what he's saying. Then we get a
snapshot of life according to Eddie this is what he hears:  "blah blah blah
Eddie, blah sit, blah blah..."

This is what I read here:

> Identification of Herpes Simplex Virus 1 blah blah blah blah blah
> Anti-Herpes blah blah blah blah blah blah blah blah blah
[quoted text clipped - 5 lines]
> blah blah blah blah blah blah blah blah blah blah herpes simplex
> virus 1 and 2 (HSV-1 and 2). The blah blah ...

(sorry Perl, but it's true!)

--
G.
Grant - 17 Jun 2004 01:27 GMT
Hahahahahahahahaha

Oh, Gadge...that's EXACTLY what I "hear" when I open one of Perl's posts.
:)

ar

> There's an episode of Frasier that comes to mind here
>
[quoted text clipped - 19 lines]
> --
> G.
Wanda - 17 Jun 2004 06:35 GMT
Hi Perl,
    This is a bit much for the regular person to wrap their mind around in
a short period of time. Sometimes I don't have but 15 minutes to take a
quick read and then I have to run. Maybe a link to the page or article?
Wanda

> Identification of Herpes Simplex Virus 1 Proteins Bound by the Novel
> Anti-Herpes Prunella vulgaris Polysaccharide
[quoted text clipped - 31 lines]
>
> http://www.cacmid.ca/abstracts/a50.html
Perl Molson - 17 Jun 2004 09:57 GMT
OK, then, let me put it this way, for all of you here:

Drinking tea (infusion) made with Prunella Vulgaris herb = good

Not drinking tea (infusion) made with Prunella Vulgaris herb = bad

Now, where can you find this herb?
Check out some asian food stores.
It's a powerful antiherpes tea. As simple as that.

I want to try also, tea made from bitter melon seed, as well.
I did not find  any such teas yet but surely there is out there a store or such
with this type of teas (I would guess philipino type of store, I read
somewhere it's popular in Philipine)

Perl Molson

> Hi Perl,
>      This is a bit much for the regular person to wrap their mind around in
[quoted text clipped - 37 lines]
> >
> > http://www.cacmid.ca/abstracts/a50.html
M.L.S. - 17 Jun 2004 15:57 GMT
>OK, then, let me put it this way, for all of you here:

>Drinking tea (infusion) made with Prunella Vulgaris herb = good

>Not drinking tea (infusion) made with Prunella Vulgaris herb = bad

Do you have herpes in your stomach, Perl?

Mike

ps.  "Nearly two dozen little known facts about tea":

http://www.angelfire.com/grrl/paghat/teafact1.html#top
Perl Molson - 17 Jun 2004 20:36 GMT
----- Original Message -----
From: "M.L.S." <msoja9@newsguy.com>
Newsgroups: alt.support.herpes
Sent: Thursday, June 17, 2004 8:57 AM
Subject: Re: Proteins Bound by Prunella vulgaris Polysaccharide

> On 17 Jun 2004 01:57:47 -0700, beatadje@email.com (Perl Molson)
> posted:
[quoted text clipped - 8 lines]
>
> Mike

Liver, kidneys' problem can be at the origin of herpes problem.

The components of teas aren't being filtrated or distroyes at the
time they reach these or other organs (blood, skin, etc)

> ps.  "Nearly two dozen little known facts about tea":
>
> http://www.angelfire.com/grrl/paghat/teafact1.html#top

http://www.planetbotanic.ca/fact_sheets/self_heal_fs.htm

Scientific Name: Prunella vulgaris

Uses: Immune

Self-heal is no secret – it's used on every continent human beings
call home. The fact that this plant is also known as heal-all and
cure-all should give you some insight into what people have found to
be true of it. They don't call it sometimes-heal, or might-heal, or
every-once-in-a-while-heal, they call it heal-all.

Self-heal is a mint relation, and as with all the other mints, if you
plant it once, you never have to plant it again. Incredibly vigorous,
the plant spreads by underground stems that shoot out in every
direction once the first root is stuck in the ground. If there is
anything to the doctrine of signatures, prunella should make anyone
who takes it into his or her body stronger than an ox.

In the southern United States, ground-hog plantain, or square weed as
it is called, is collected in the spring as a tonic plant. It is also
eaten as a spinach substitute, prepared in a big pot with a piece of
hog meat by cooks who follow the traditional southern style or with a
little vegetable oil by more modern cooks concerned with the bad
publicity animal fats have received in recent years.

--------------------------------------------------------------------------------

Self-heal has a widespread reputation for keeping people well during
an outbreak of infectious disease. This, of course, makes it perfect
for life in the modern world.

--------------------------------------------------------------------------------

In China, where, as it does worldwide, the plant grows great guns, it
is called hsia-ku-tsao and is widely used as a tonic. The Chinese, who
collect the plant as it spikes into bloom, take the lower leaves and
flower heads from it to treat fevers and rheumatism. They also use the
leaves and flower heads as an alterative, a substance which helps the
body change from a state of sickness to one of health. When self-heal
is taken in tea, the Chinese feel that the plant can keep the entire
body well. It is said to assist liver function, resulting in bright,
clear eyes. As such, self-heal is used to treat patients suffering
from eye or liver trouble. It is also used to treat lumps in the neck
and swollen glands. This ties in with its use as an immunity booster:
when your body gets run down, those lymph glands swell right up.

Knowing the Chinese use self-heal as an alterative, we should not be
surprised to learn that the plant has an antibiotic effect.
Experiments indicate that self-heal has broad antimicrobial powers and
also kills many pathogenic fungi, the kind that attack the body and do
you no good.

Self-heal is well known in Europe, and our friend Gerard had
additional uses to list, noting that "the decoction of Prunell made
with wine or water, doth joine together and make whole and sound all
wounds, both inward and outward, even as Bugle doth." His reference to
the plant's ability to make things whole after the body has suffered
both external and internal injuries is consistent with other European
sources: as the name says, the plant helps healing. Gerard also wrote
of prunella's potency as a headache treatment when "bruised with oil
of roses and vinegar, and laid to the forepart of the head," and he
recommended the plant "against the infirmities of the mouth, and
especially the ruggedness, blackness, and dryness of the tongue, with
a kind of swelling in the same. It is an infirmitie amongst soldiers
that lie in campe."

I'll tell you one thing: if I ever woke up and found I had a black
tongue, I would get real nervous fast. Having your tongue turn black
is Mother Nature's way of saying that you need to work on your health
regime. Gerard's reference to soldiers "that lie in campe" is
significant. Soldiers live in close quarters, and when a sickness hits
the barracks, it spreads like wildfire, kind of like a cold running
through the office. Self-heal has a widespread reputation for keeping
people well during an outbreak of infectious disease. This, of course,
makes it perfect for life in the modern world.

In colonial America, self-heal should have been called
heal-anything-you've-got, as its uses were incredibly diverse. It was
used to treat sore throats, stomach cramps, and urinary and liver
problems. It was also prescribed to kill worms and to help folks who
suffered from fits. Its main use though was as a tonic.

The Shakers sold lots of self-heal to treat internal bleeding, sore
throats, and cankers in the mouth. Also in agreement with Gerard, they
believed that self-heal was good for black tongues and cold sores. The
gypsies of Eastern Europe, who gave a double ditto on self-heal's
ability to cure sore throats, used it as an ingredient in their
medicine show tonics for that problem.

In New Zealand the plant gets wide use as a first aid ointment – the
ground plant is applied to cuts, wounds, bruises, and sores that won't
heal. The Kiwis are not alone in the thought that whatever the plant
touches heals a lot faster than it otherwise would. Like Gerard, they
say that putting the juice of its leaves and flowers on the temples
will take care of a headache in short order.

Most would agree that the heart is an organ we would like to keep
pumping away, trouble-free. Self-heal is featured in an Irish
heart-disease treatment called Cailleach's Tea. Chinese researchers
have found the plant to be an effective remedy for hypertension, a
fact that would indeed make Cailleach's Tea useful for someone whose
heart troubles stem from high blood pressure.

"Heal-all" is a steep claim, but even if it's only partially true, we
would all be better off with self-heal prunella in our tonic pot. As
I've already mentioned, growing it, or more accurately letting it grow
itself, is no problem. Stick some in the ground and stand back. When
you are harvesting self-heal for the tonic pot, cut the plant as it
breaks into bloom, trimming it off one inch above the roots. The plant
won't mind – as a matter of fact, this gives it an incentive to grow
more.

> OK, then, let me put it this way, for all of you here:
>
[quoted text clipped - 54 lines]
> > >
> > > http://www.cacmid.ca/abstracts/a50.html
Perl Molson - 17 Jun 2004 21:03 GMT
----- Original Message -----
From: "M.L.S." <msoja9@newsguy.com>
Newsgroups: alt.support.herpes
Sent: Thursday, June 17, 2004 8:57 AM
Subject: Re: Proteins Bound by Prunella vulgaris Polysaccharide

> On 17 Jun 2004 01:57:47 -0700, beatadje@email.com (Perl Molson)
> posted:
[quoted text clipped - 6 lines]
>
> Do you have herpes in your stomach, Perl?

Mike, you see, that's where your own prejudices are
hiding the truth from you.

The real origin of a certain disease that is manifesting itself
on the lips or in the genital areas can be in the liver, kidneys,
intestines, etc.

At the time the tea components are reaching those organs, they are
not much different from their original form in the plant.
Even after being filtered by the kidneys, liver and all that,
they can still reach the blood, the skin, etc.

Perl Molson

> Mike
>
> ps.  "Nearly two dozen little known facts about tea":
>
> http://www.angelfire.com/grrl/paghat/teafact1.html#top

http://www.planetbotanic.ca/fact_sheets/self_heal_fs.htm
Scientific Name: Prunella vulgaris

Uses: Immune

Self-heal is no secret – it's used on every continent human beings
call home. The fact that this plant is also known as heal-all and
cure-all should give you some insight into what people have found to
be true of it. They don't call it sometimes-heal, or might-heal, or
every-once-in-a-while-heal, they call it heal-all.

Self-heal is a mint relation, and as with all the other mints, if you
plant it once, you never have to plant it again. Incredibly vigorous,
the plant spreads by underground stems that shoot out in every
direction once the first root is stuck in the ground. If there is
anything to the doctrine of signatures, prunella should make anyone
who takes it into his or her body stronger than an ox.

In the southern United States, ground-hog plantain, or square weed as
it is called, is collected in the spring as a tonic plant. It is also
eaten as a spinach substitute, prepared in a big pot with a piece of
hog meat by cooks who follow the traditional southern style or with a
little vegetable oil by more modern cooks concerned with the bad
publicity animal fats have received in recent years.

--------------------------------------------------------------------------------

Self-heal has a widespread reputation for keeping people well during
an outbreak of infectious disease. This, of course, makes it perfect
for life in the modern world.

--------------------------------------------------------------------------------

In China, where, as it does worldwide, the plant grows great guns, it
is called hsia-ku-tsao and is widely used as a tonic. The Chinese, who
collect the plant as it spikes into bloom, take the lower leaves and
flower heads from it to treat fevers and rheumatism. They also use the
leaves and flower heads as an alterative, a substance which helps the
body change from a state of sickness to one of health. When self-heal
is taken in tea, the Chinese feel that the plant can keep the entire
body well. It is said to assist liver function, resulting in bright,
clear eyes. As such, self-heal is used to treat patients suffering
from eye or liver trouble. It is also used to treat lumps in the neck
and swollen glands. This ties in with its use as an immunity booster:
when your body gets run down, those lymph glands swell right up.

Knowing the Chinese use self-heal as an alterative, we should not be
surprised to learn that the plant has an antibiotic effect.
Experiments indicate that self-heal has broad antimicrobial powers and
also kills many pathogenic fungi, the kind that attack the body and do
you no good.

Self-heal is well known in Europe, and our friend Gerard had
additional uses to list, noting that "the decoction of Prunell made
with wine or water, doth joine together and make whole and sound all
wounds, both inward and outward, even as Bugle doth." His reference to
the plant's ability to make things whole after the body has suffered
both external and internal injuries is consistent with other European
sources: as the name says, the plant helps healing. Gerard also wrote
of prunella's potency as a headache treatment when "bruised with oil
of roses and vinegar, and laid to the forepart of the head," and he
recommended the plant "against the infirmities of the mouth, and
especially the ruggedness, blackness, and dryness of the tongue, with
a kind of swelling in the same. It is an infirmitie amongst soldiers
that lie in campe."

I'll tell you one thing: if I ever woke up and found I had a black
tongue, I would get real nervous fast. Having your tongue turn black
is Mother Nature's way of saying that you need to work on your health
regime. Gerard's reference to soldiers "that lie in campe" is
significant. Soldiers live in close quarters, and when a sickness hits
the barracks, it spreads like wildfire, kind of like a cold running
through the office. Self-heal has a widespread reputation for keeping
people well during an outbreak of infectious disease. This, of course,
makes it perfect for life in the modern world.

In colonial America, self-heal should have been called
heal-anything-you've-got, as its uses were incredibly diverse. It was
used to treat sore throats, stomach cramps, and urinary and liver
problems. It was also prescribed to kill worms and to help folks who
suffered from fits. Its main use though was as a tonic.

The Shakers sold lots of self-heal to treat internal bleeding, sore
throats, and cankers in the mouth. Also in agreement with Gerard, they
believed that self-heal was good for black tongues and cold sores. The
gypsies of Eastern Europe, who gave a double ditto on self-heal's
ability to cure sore throats, used it as an ingredient in their
medicine show tonics for that problem.

In New Zealand the plant gets wide use as a first aid ointment – the
ground plant is applied to cuts, wounds, bruises, and sores that won't
heal. The Kiwis are not alone in the thought that whatever the plant
touches heals a lot faster than it otherwise would. Like Gerard, they
say that putting the juice of its leaves and flowers on the temples
will take care of a headache in short order.

Most would agree that the heart is an organ we would like to keep
pumping away, trouble-free. Self-heal is featured in an Irish
heart-disease treatment called Cailleach's Tea. Chinese researchers
have found the plant to be an effective remedy for hypertension, a
fact that would indeed make Cailleach's Tea useful for someone whose
heart troubles stem from high blood pressure.

"Heal-all" is a steep claim, but even if it's only partially true, we
would all be better off with self-heal prunella in our tonic pot. As
I've already mentioned, growing it, or more accurately letting it grow
itself, is no problem. Stick some in the ground and stand back. When
you are harvesting self-heal for the tonic pot, cut the plant as it
breaks into bloom, trimming it off one inch above the roots. The plant
won't mind – as a matter of fact, this gives it an incentive to grow
more.

> OK, then, let me put it this way, for all of you here:
>
[quoted text clipped - 54 lines]
> > >
> > > http://www.cacmid.ca/abstracts/a50.html
M.L.S. - 17 Jun 2004 21:36 GMT
>From: "M.L.S." <msoja9@newsguy.com>

>> On 17 Jun 2004 01:57:47 -0700, beatadje@email.com (Perl Molson)
>> posted:

>> >Drinking tea (infusion) made with Prunella Vulgaris herb = good

>> >Not drinking tea (infusion) made with Prunella Vulgaris herb = bad

>> Do you have herpes in your stomach, Perl?

>Mike, you see, that's where your own prejudices are
>hiding the truth from you.

What nonsense through yonder window breaks, now?

>The real origin of a certain disease that is manifesting itself
>on the lips or in the genital areas can be in the liver, kidneys,
>intestines, etc.

Well, Perl, Science tells us that Herpes is caused by a virus that
takes up residence in the nerve ganglia associated with our oral or
genital regions, so I have no idea what you think you're talking
about.  Maybe all the nutty stuff you ingest has given you
indigestion?

>At the time the tea components are reaching those organs, they are
>not much different from their original form in the plant.
>Even after being filtered by the kidneys, liver and all that,
>they can still reach the blood, the skin, etc.

blah blah blah blah blah blah blah blah blah blah blah blah blah
blah blah blah blah blah blah blah blah blah blah blah blah blah

Mike
Perl Molson - 18 Jun 2004 23:54 GMT
> >From: "M.L.S." <msoja9@newsguy.com>
>  
[quoted text clipped - 21 lines]
> about.  Maybe all the nutty stuff you ingest has given you
> indigestion?

To understand these things you need some knowledge of how
some things work. If you can't figure them out, sorry then I can't
help you there.

Where some of the viral DNA and other things have their residence
doesn't tell a heck of a lot about how a certain treatment would work.

There is a lot of interconnectivity between our bodies' parts.
Think about sex for a minute...

> >At the time the tea components are reaching those organs, they are
> >not much different from their original form in the plant.
[quoted text clipped - 5 lines]
>
> Mike

Try eating just a couple of cloves of garlic and you'll see how garlic's oils
will reach the skin pretty fast (use some gloves when you cut the garlic if you
need a better proof).

Perl Molson
Gadge - 18 Jun 2004 00:08 GMT
> OK, then, let me put it this way, for all of you here:
>
[quoted text clipped - 12 lines]
>
> Perl Molson

Thanks Perl - that's much better.

Of course you still have to put the link in to anyone that is interested
(and more people may well be if you give a summary) can go and check it out
for themselves.  Otherwise we only have you word for this, and I'm always
suspicious of anything I read if I can't validate the information.

Now if only I could get people at work to summarise their emails in a
simliar manner (am sick of getting long emails forwarded on with just a
simple FYI at the top - like I'm going to want to trawl through the whole
thing for one small relevant point?)

--
G.
Perl Molson - 18 Jun 2004 23:57 GMT
> > OK, then, let me put it this way, for all of you here:
> >
[quoted text clipped - 25 lines]
> simple FYI at the top - like I'm going to want to trawl through the whole
> thing for one small relevant point?)

The main reason that I am posting some things in here,
is because I can have a clearer "window" in the context of
trying to understand how herpes virus works, that is, in here at ASH.

Thus, some of the things are only my personal reference and
the only reason I don't put it as off topic is because
I thought that everyone can figure this out on their own.

Thanks for understanding.
Perl Molson
Gadge - 21 Jun 2004 21:46 GMT
> > > OK, then, let me put it this way, for all of you here:
> > >
[quoted text clipped - 35 lines]
>
> Thanks for understanding.

That all makes sense, but if you have lots of information, and you can post
a link and a summary.  It would really help people like me that don't have
broadband - that I can decide  if I want to spend the time downloading the
information.

Cheers

--
G
Perl Molson - 17 Jun 2004 20:47 GMT
http://www.skinchoice.com/herpasil.htm

> Identification of Herpes Simplex Virus 1 Proteins Bound by the Novel
> Anti-Herpes Prunella vulgaris Polysaccharide
[quoted text clipped - 31 lines]
>
> http://www.cacmid.ca/abstracts/a50.html
 
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