Medical Forum / Diseases and Disorders / Herpes / June 2004
Proteins Bound by Prunella vulgaris Polysaccharide
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Perl Molson - 16 Jun 2004 19:22 GMT Identification of Herpes Simplex Virus 1 Proteins Bound by the Novel Anti-Herpes Prunella vulgaris Polysaccharide G. DELANEY1, S. H. S. LEE1*, AND S. F. LEE1,2. 1Dept. of Microbiology & Immunology, 2Dept. of Applied Oral Sciences, Dalhousie University, Halifax, Nova Scotia.
Objective: We previously described a polysaccharide from the Chinese herb Prunella vulgaris exhibiting activity against herpes simplex virus 1 and 2 (HSV-1 and 2). The polysaccharide apparently inhibited viral binding as well as events post-viral binding and penetration. The objective of this study is to identify the viral proteins bound by the Prunella polysaccharide.
Methods: [35S]methionine-labeled proteins were obtained from HSV-1-infected Vero cell lysates by freeze-thaw cycles and Noindet P-40 and sodium deoxycholate treatment. The proteins were applied to a Prunella polysaccharide-Sepharose column. Bound proteins were eluted by Prunella polysaccharide, immuno-precipitated by anti-gC and anti-gD antibodies and analyzed by SDS-PAGE.
Results: Following chromatography on the Prunella polysaccharide affinity column, two broad bands of 120-112 kDa and 69- 74 kDa were obtained from the HSV-1 infected lysate; these protein bands were absent from the mock infected lysate suggesting that the proteins were of viral in origin. Similar proteins were obtained when sucrose-gradient purified HSV-1 lysate was used. Two protein bands of 115 kDa and 74 kDa were recovered from the eluted proteins by immuno-precipitation with the anti-gC antibody, while a 68 kDa protein band was precipitated by the anti-gD antibody. The 115 kDa and 68 kDa proteins corresponded to the reported size of gC and gD, respectively. The nature of the 74 kDa is unclear but it could be a truncated version of the gC or another HSV-1 protein which shared common antigenic epitopes with gC.
http://www.cacmid.ca/abstracts/a50.html
Gadge - 16 Jun 2004 23:16 GMT There's an episode of Frasier that comes to mind here
It's the one where Frasier's dad reckon his dog is way more intelligent than the average dog and can understand much of what he's saying. Then we get a snapshot of life according to Eddie this is what he hears: "blah blah blah Eddie, blah sit, blah blah..."
This is what I read here:
> Identification of Herpes Simplex Virus 1 blah blah blah blah blah > Anti-Herpes blah blah blah blah blah blah blah blah blah [quoted text clipped - 5 lines] > blah blah blah blah blah blah blah blah blah blah herpes simplex > virus 1 and 2 (HSV-1 and 2). The blah blah ... (sorry Perl, but it's true!)
-- G.
Grant - 17 Jun 2004 01:27 GMT Hahahahahahahahaha
Oh, Gadge...that's EXACTLY what I "hear" when I open one of Perl's posts.
:) ar
> There's an episode of Frasier that comes to mind here > [quoted text clipped - 19 lines] > -- > G. Wanda - 17 Jun 2004 06:35 GMT Hi Perl, This is a bit much for the regular person to wrap their mind around in a short period of time. Sometimes I don't have but 15 minutes to take a quick read and then I have to run. Maybe a link to the page or article? Wanda
> Identification of Herpes Simplex Virus 1 Proteins Bound by the Novel > Anti-Herpes Prunella vulgaris Polysaccharide [quoted text clipped - 31 lines] > > http://www.cacmid.ca/abstracts/a50.html Perl Molson - 17 Jun 2004 09:57 GMT OK, then, let me put it this way, for all of you here:
Drinking tea (infusion) made with Prunella Vulgaris herb = good
Not drinking tea (infusion) made with Prunella Vulgaris herb = bad
Now, where can you find this herb? Check out some asian food stores. It's a powerful antiherpes tea. As simple as that.
I want to try also, tea made from bitter melon seed, as well. I did not find any such teas yet but surely there is out there a store or such with this type of teas (I would guess philipino type of store, I read somewhere it's popular in Philipine)
Perl Molson
> Hi Perl, > This is a bit much for the regular person to wrap their mind around in [quoted text clipped - 37 lines] > > > > http://www.cacmid.ca/abstracts/a50.html M.L.S. - 17 Jun 2004 15:57 GMT >OK, then, let me put it this way, for all of you here:
>Drinking tea (infusion) made with Prunella Vulgaris herb = good
>Not drinking tea (infusion) made with Prunella Vulgaris herb = bad Do you have herpes in your stomach, Perl?
Mike
ps. "Nearly two dozen little known facts about tea":
http://www.angelfire.com/grrl/paghat/teafact1.html#top
Perl Molson - 17 Jun 2004 20:36 GMT ----- Original Message ----- From: "M.L.S." <msoja9@newsguy.com> Newsgroups: alt.support.herpes Sent: Thursday, June 17, 2004 8:57 AM Subject: Re: Proteins Bound by Prunella vulgaris Polysaccharide
> On 17 Jun 2004 01:57:47 -0700, beatadje@email.com (Perl Molson) > posted: [quoted text clipped - 8 lines] > > Mike Liver, kidneys' problem can be at the origin of herpes problem.
The components of teas aren't being filtrated or distroyes at the time they reach these or other organs (blood, skin, etc)
> ps. "Nearly two dozen little known facts about tea": > > http://www.angelfire.com/grrl/paghat/teafact1.html#top http://www.planetbotanic.ca/fact_sheets/self_heal_fs.htm
Scientific Name: Prunella vulgaris
Uses: Immune
Self-heal is no secret it's used on every continent human beings call home. The fact that this plant is also known as heal-all and cure-all should give you some insight into what people have found to be true of it. They don't call it sometimes-heal, or might-heal, or every-once-in-a-while-heal, they call it heal-all.
Self-heal is a mint relation, and as with all the other mints, if you plant it once, you never have to plant it again. Incredibly vigorous, the plant spreads by underground stems that shoot out in every direction once the first root is stuck in the ground. If there is anything to the doctrine of signatures, prunella should make anyone who takes it into his or her body stronger than an ox.
In the southern United States, ground-hog plantain, or square weed as it is called, is collected in the spring as a tonic plant. It is also eaten as a spinach substitute, prepared in a big pot with a piece of hog meat by cooks who follow the traditional southern style or with a little vegetable oil by more modern cooks concerned with the bad publicity animal fats have received in recent years.
--------------------------------------------------------------------------------
Self-heal has a widespread reputation for keeping people well during an outbreak of infectious disease. This, of course, makes it perfect for life in the modern world.
--------------------------------------------------------------------------------
In China, where, as it does worldwide, the plant grows great guns, it is called hsia-ku-tsao and is widely used as a tonic. The Chinese, who collect the plant as it spikes into bloom, take the lower leaves and flower heads from it to treat fevers and rheumatism. They also use the leaves and flower heads as an alterative, a substance which helps the body change from a state of sickness to one of health. When self-heal is taken in tea, the Chinese feel that the plant can keep the entire body well. It is said to assist liver function, resulting in bright, clear eyes. As such, self-heal is used to treat patients suffering from eye or liver trouble. It is also used to treat lumps in the neck and swollen glands. This ties in with its use as an immunity booster: when your body gets run down, those lymph glands swell right up.
Knowing the Chinese use self-heal as an alterative, we should not be surprised to learn that the plant has an antibiotic effect. Experiments indicate that self-heal has broad antimicrobial powers and also kills many pathogenic fungi, the kind that attack the body and do you no good.
Self-heal is well known in Europe, and our friend Gerard had additional uses to list, noting that "the decoction of Prunell made with wine or water, doth joine together and make whole and sound all wounds, both inward and outward, even as Bugle doth." His reference to the plant's ability to make things whole after the body has suffered both external and internal injuries is consistent with other European sources: as the name says, the plant helps healing. Gerard also wrote of prunella's potency as a headache treatment when "bruised with oil of roses and vinegar, and laid to the forepart of the head," and he recommended the plant "against the infirmities of the mouth, and especially the ruggedness, blackness, and dryness of the tongue, with a kind of swelling in the same. It is an infirmitie amongst soldiers that lie in campe."
I'll tell you one thing: if I ever woke up and found I had a black tongue, I would get real nervous fast. Having your tongue turn black is Mother Nature's way of saying that you need to work on your health regime. Gerard's reference to soldiers "that lie in campe" is significant. Soldiers live in close quarters, and when a sickness hits the barracks, it spreads like wildfire, kind of like a cold running through the office. Self-heal has a widespread reputation for keeping people well during an outbreak of infectious disease. This, of course, makes it perfect for life in the modern world.
In colonial America, self-heal should have been called heal-anything-you've-got, as its uses were incredibly diverse. It was used to treat sore throats, stomach cramps, and urinary and liver problems. It was also prescribed to kill worms and to help folks who suffered from fits. Its main use though was as a tonic.
The Shakers sold lots of self-heal to treat internal bleeding, sore throats, and cankers in the mouth. Also in agreement with Gerard, they believed that self-heal was good for black tongues and cold sores. The gypsies of Eastern Europe, who gave a double ditto on self-heal's ability to cure sore throats, used it as an ingredient in their medicine show tonics for that problem.
In New Zealand the plant gets wide use as a first aid ointment the ground plant is applied to cuts, wounds, bruises, and sores that won't heal. The Kiwis are not alone in the thought that whatever the plant touches heals a lot faster than it otherwise would. Like Gerard, they say that putting the juice of its leaves and flowers on the temples will take care of a headache in short order.
Most would agree that the heart is an organ we would like to keep pumping away, trouble-free. Self-heal is featured in an Irish heart-disease treatment called Cailleach's Tea. Chinese researchers have found the plant to be an effective remedy for hypertension, a fact that would indeed make Cailleach's Tea useful for someone whose heart troubles stem from high blood pressure.
"Heal-all" is a steep claim, but even if it's only partially true, we would all be better off with self-heal prunella in our tonic pot. As I've already mentioned, growing it, or more accurately letting it grow itself, is no problem. Stick some in the ground and stand back. When you are harvesting self-heal for the tonic pot, cut the plant as it breaks into bloom, trimming it off one inch above the roots. The plant won't mind as a matter of fact, this gives it an incentive to grow more.
> OK, then, let me put it this way, for all of you here: > [quoted text clipped - 54 lines] > > > > > > http://www.cacmid.ca/abstracts/a50.html Perl Molson - 17 Jun 2004 21:03 GMT ----- Original Message ----- From: "M.L.S." <msoja9@newsguy.com> Newsgroups: alt.support.herpes Sent: Thursday, June 17, 2004 8:57 AM Subject: Re: Proteins Bound by Prunella vulgaris Polysaccharide
> On 17 Jun 2004 01:57:47 -0700, beatadje@email.com (Perl Molson) > posted: [quoted text clipped - 6 lines] > > Do you have herpes in your stomach, Perl? Mike, you see, that's where your own prejudices are hiding the truth from you.
The real origin of a certain disease that is manifesting itself on the lips or in the genital areas can be in the liver, kidneys, intestines, etc.
At the time the tea components are reaching those organs, they are not much different from their original form in the plant. Even after being filtered by the kidneys, liver and all that, they can still reach the blood, the skin, etc.
Perl Molson
> Mike > > ps. "Nearly two dozen little known facts about tea": > > http://www.angelfire.com/grrl/paghat/teafact1.html#top http://www.planetbotanic.ca/fact_sheets/self_heal_fs.htm Scientific Name: Prunella vulgaris
Uses: Immune
Self-heal is no secret it's used on every continent human beings call home. The fact that this plant is also known as heal-all and cure-all should give you some insight into what people have found to be true of it. They don't call it sometimes-heal, or might-heal, or every-once-in-a-while-heal, they call it heal-all.
Self-heal is a mint relation, and as with all the other mints, if you plant it once, you never have to plant it again. Incredibly vigorous, the plant spreads by underground stems that shoot out in every direction once the first root is stuck in the ground. If there is anything to the doctrine of signatures, prunella should make anyone who takes it into his or her body stronger than an ox.
In the southern United States, ground-hog plantain, or square weed as it is called, is collected in the spring as a tonic plant. It is also eaten as a spinach substitute, prepared in a big pot with a piece of hog meat by cooks who follow the traditional southern style or with a little vegetable oil by more modern cooks concerned with the bad publicity animal fats have received in recent years.
--------------------------------------------------------------------------------
Self-heal has a widespread reputation for keeping people well during an outbreak of infectious disease. This, of course, makes it perfect for life in the modern world.
--------------------------------------------------------------------------------
In China, where, as it does worldwide, the plant grows great guns, it is called hsia-ku-tsao and is widely used as a tonic. The Chinese, who collect the plant as it spikes into bloom, take the lower leaves and flower heads from it to treat fevers and rheumatism. They also use the leaves and flower heads as an alterative, a substance which helps the body change from a state of sickness to one of health. When self-heal is taken in tea, the Chinese feel that the plant can keep the entire body well. It is said to assist liver function, resulting in bright, clear eyes. As such, self-heal is used to treat patients suffering from eye or liver trouble. It is also used to treat lumps in the neck and swollen glands. This ties in with its use as an immunity booster: when your body gets run down, those lymph glands swell right up.
Knowing the Chinese use self-heal as an alterative, we should not be surprised to learn that the plant has an antibiotic effect. Experiments indicate that self-heal has broad antimicrobial powers and also kills many pathogenic fungi, the kind that attack the body and do you no good.
Self-heal is well known in Europe, and our friend Gerard had additional uses to list, noting that "the decoction of Prunell made with wine or water, doth joine together and make whole and sound all wounds, both inward and outward, even as Bugle doth." His reference to the plant's ability to make things whole after the body has suffered both external and internal injuries is consistent with other European sources: as the name says, the plant helps healing. Gerard also wrote of prunella's potency as a headache treatment when "bruised with oil of roses and vinegar, and laid to the forepart of the head," and he recommended the plant "against the infirmities of the mouth, and especially the ruggedness, blackness, and dryness of the tongue, with a kind of swelling in the same. It is an infirmitie amongst soldiers that lie in campe."
I'll tell you one thing: if I ever woke up and found I had a black tongue, I would get real nervous fast. Having your tongue turn black is Mother Nature's way of saying that you need to work on your health regime. Gerard's reference to soldiers "that lie in campe" is significant. Soldiers live in close quarters, and when a sickness hits the barracks, it spreads like wildfire, kind of like a cold running through the office. Self-heal has a widespread reputation for keeping people well during an outbreak of infectious disease. This, of course, makes it perfect for life in the modern world.
In colonial America, self-heal should have been called heal-anything-you've-got, as its uses were incredibly diverse. It was used to treat sore throats, stomach cramps, and urinary and liver problems. It was also prescribed to kill worms and to help folks who suffered from fits. Its main use though was as a tonic.
The Shakers sold lots of self-heal to treat internal bleeding, sore throats, and cankers in the mouth. Also in agreement with Gerard, they believed that self-heal was good for black tongues and cold sores. The gypsies of Eastern Europe, who gave a double ditto on self-heal's ability to cure sore throats, used it as an ingredient in their medicine show tonics for that problem.
In New Zealand the plant gets wide use as a first aid ointment the ground plant is applied to cuts, wounds, bruises, and sores that won't heal. The Kiwis are not alone in the thought that whatever the plant touches heals a lot faster than it otherwise would. Like Gerard, they say that putting the juice of its leaves and flowers on the temples will take care of a headache in short order.
Most would agree that the heart is an organ we would like to keep pumping away, trouble-free. Self-heal is featured in an Irish heart-disease treatment called Cailleach's Tea. Chinese researchers have found the plant to be an effective remedy for hypertension, a fact that would indeed make Cailleach's Tea useful for someone whose heart troubles stem from high blood pressure.
"Heal-all" is a steep claim, but even if it's only partially true, we would all be better off with self-heal prunella in our tonic pot. As I've already mentioned, growing it, or more accurately letting it grow itself, is no problem. Stick some in the ground and stand back. When you are harvesting self-heal for the tonic pot, cut the plant as it breaks into bloom, trimming it off one inch above the roots. The plant won't mind as a matter of fact, this gives it an incentive to grow more.
> OK, then, let me put it this way, for all of you here: > [quoted text clipped - 54 lines] > > > > > > http://www.cacmid.ca/abstracts/a50.html M.L.S. - 17 Jun 2004 21:36 GMT >From: "M.L.S." <msoja9@newsguy.com>
>> On 17 Jun 2004 01:57:47 -0700, beatadje@email.com (Perl Molson) >> posted:
>> >Drinking tea (infusion) made with Prunella Vulgaris herb = good
>> >Not drinking tea (infusion) made with Prunella Vulgaris herb = bad
>> Do you have herpes in your stomach, Perl?
>Mike, you see, that's where your own prejudices are >hiding the truth from you. What nonsense through yonder window breaks, now?
>The real origin of a certain disease that is manifesting itself >on the lips or in the genital areas can be in the liver, kidneys, >intestines, etc. Well, Perl, Science tells us that Herpes is caused by a virus that takes up residence in the nerve ganglia associated with our oral or genital regions, so I have no idea what you think you're talking about. Maybe all the nutty stuff you ingest has given you indigestion?
>At the time the tea components are reaching those organs, they are >not much different from their original form in the plant. >Even after being filtered by the kidneys, liver and all that, >they can still reach the blood, the skin, etc. blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah
Mike
Perl Molson - 18 Jun 2004 23:54 GMT > >From: "M.L.S." <msoja9@newsguy.com> > [quoted text clipped - 21 lines] > about. Maybe all the nutty stuff you ingest has given you > indigestion? To understand these things you need some knowledge of how some things work. If you can't figure them out, sorry then I can't help you there.
Where some of the viral DNA and other things have their residence doesn't tell a heck of a lot about how a certain treatment would work.
There is a lot of interconnectivity between our bodies' parts. Think about sex for a minute...
> >At the time the tea components are reaching those organs, they are > >not much different from their original form in the plant. [quoted text clipped - 5 lines] > > Mike Try eating just a couple of cloves of garlic and you'll see how garlic's oils will reach the skin pretty fast (use some gloves when you cut the garlic if you need a better proof).
Perl Molson
Gadge - 18 Jun 2004 00:08 GMT > OK, then, let me put it this way, for all of you here: > [quoted text clipped - 12 lines] > > Perl Molson Thanks Perl - that's much better.
Of course you still have to put the link in to anyone that is interested (and more people may well be if you give a summary) can go and check it out for themselves. Otherwise we only have you word for this, and I'm always suspicious of anything I read if I can't validate the information.
Now if only I could get people at work to summarise their emails in a simliar manner (am sick of getting long emails forwarded on with just a simple FYI at the top - like I'm going to want to trawl through the whole thing for one small relevant point?)
-- G.
Perl Molson - 18 Jun 2004 23:57 GMT > > OK, then, let me put it this way, for all of you here: > > [quoted text clipped - 25 lines] > simple FYI at the top - like I'm going to want to trawl through the whole > thing for one small relevant point?) The main reason that I am posting some things in here, is because I can have a clearer "window" in the context of trying to understand how herpes virus works, that is, in here at ASH.
Thus, some of the things are only my personal reference and the only reason I don't put it as off topic is because I thought that everyone can figure this out on their own.
Thanks for understanding. Perl Molson
Gadge - 21 Jun 2004 21:46 GMT > > > OK, then, let me put it this way, for all of you here: > > > [quoted text clipped - 35 lines] > > Thanks for understanding. That all makes sense, but if you have lots of information, and you can post a link and a summary. It would really help people like me that don't have broadband - that I can decide if I want to spend the time downloading the information.
Cheers
-- G
Perl Molson - 17 Jun 2004 20:47 GMT http://www.skinchoice.com/herpasil.htm
> Identification of Herpes Simplex Virus 1 Proteins Bound by the Novel > Anti-Herpes Prunella vulgaris Polysaccharide [quoted text clipped - 31 lines] > > http://www.cacmid.ca/abstracts/a50.html
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