Medical Forum / Diseases and Disorders / Epilepsy / March 2005
Taurine / metal-stimulated oxidation
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ironjustice@aol.com - 27 Feb 2005 20:01 GMT Neurotox Res. 2000 Jan;2(1):1-15. Related Articles, Links
Taurine inhibition of metal-stimulated catecholamine oxidation.
Dawson R Jr, Baker D, Eppler B, Tang E, Shih D, Hern H, Hu M.
Department of Pharmacodynamics, College of Pharmacy, JHMHC Box 100487, University of Florida, Gainesville, FL 32610, USA.
Taurine is an abundant amino acid found in mammalian tissues and it has been suggested to have cytoprotective functions. The aim of the present study was to determine if taurine had the potential to reduce oxidative stress associated with metal-stimulated catecholamine oxidation. Taurine and structural analogs of taurine were tested for their ability to inhibit metal-stimulated quinone formation from dopamine or L-dopa. Oxidative damage to proteins and lipids were also assessed in vitro and the effects of taurine were determined. Taurine (20 mM) was found to decrease significantly ferric iron (50-500 microM)- and manganese (10 microM)-stimulated L-dopa or dopamine oxidation. Taurine had no effect on zinc-induced dopamine oxidation and slightly potentiated copper- and NaIO(4)-stimulated quinone formation. Ferric iron-stimulated lipid peroxidation was not affected by taurine (1-20 mM). Protein carbonyl formation induced by ferric iron (500 microM) and L-dopa (500 microM) was significantly reduced by 10 mM taurine. The cytotoxicity of L-dopa (250 microM) and ferric chloride (75 microM) to LLC-PK(1) cells was attenuated by 10 mM taurine or hypotaurine. Homotaurine alone stimulated L-dopa oxidation and potentiated the cytotoxic effects of ferric iron. Homotaurine was found to be cytotoxic when combined with L-dopa or L-dopa/iron. In contrast, hypotaurine inhibited quinone formation and protected LLC-PK(1) cells. These studies suggest that taurine may exhibit cytoprotective effects against the oxidation products of catecholamines by acting as a scavenger for free radicals and cytotoxic quinones.
PMID: 15545001 [PubMed - in process]
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ironjustice@aol.com - 28 Feb 2005 15:41 GMT Amino Acids. 2002 Jun;22(4):351-68. Related Articles, Links
Effects of sulfur containing amino acids on iron and nitric oxide stimulated catecholamine oxidation.
Biasetti M, Dawson R Jr.
Department of Pharmacodynamics, College of Pharmacy, JHMHC Box 100487, University of Florida, Gainesville, FL 32610, U.S.A.
Taurine is a free amino acid found in high concentrations in tissues containing catecholamines. The ability of taurine and its metabolic precursors to inhibit or stimulate catecholamine oxidation and subsequent quinone formation was examined. Ferric chloride was used as the catalyzing agent to stimulate L-dopa or norepinephrine oxidation and NO donors were also examined for their actions to stimulate quinone formation. Taurine attenuated iron-stimulated quinone formation from catecholamines suggesting that it may function as an endogenous antioxidant. Several other sulfur-containing amino acids (homocysteic acid, cysteine sulfinic acid and SAM) were found to inhibit catecholamine oxidation. Among other amino acids tested, homocysteine had biphasic effects; attenuating L-dopa oxidation catalyzed by ferric chloride and potentiating norepinephrine's oxidation catalyzed by both ferric chloride and sodium nitroprusside (SNP). Homotaurine and homocysteine (1 or 10 mM) greatly stimulated SNP-induced norepinephrine oxidation. Homotaurine potentiated quinone formation in the presence of ferric iron and this effect was attenuated by desferroxamine. In order to exclude a possible NO/iron interaction in SNP's oxidizing action, SIN-1 chloride, a specific NO-donor, was tested as an oxidizing agent. The failure of desferroxamine or taurine to attenuate SIN-1 oxidation of norepinephrine suggests that peroxynitrite-mediated oxidation was likely the dominant mechanism. Our results show that endogenous sulfur containing amino acids, like taurine, could serve a protective role to reduce cellular damage associated with both NO and metal-stimulated catecholamine oxidation.
PMID: 12107762 [PubMed - in process]
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PIPER - 01 Mar 2005 04:17 GMT Some of this I can understand but not all, could you put in layman (sp?) terms please?
thanks
piper hall
Michael - 01 Mar 2005 06:25 GMT PIPER said:
> Some of this I can understand but not all, could you put in layman > (sp?) terms please? Your0 request isn't an easy one to fulfill.
Problem: In order to explain it it in layman's terms, Tom would have first to understand it.
He doesn't, of course... hence the difficulty.
May I suggest at this point that you'd be more swiftly satisfied by asking about the requisite number of angels for pinhead-dancing?
PIPER - 01 Mar 2005 10:23 GMT Ah I see, so he isnt legit. Got it, thanks.This kind of caught my eye since i have heard about taurine for blood sugar control.
piper
> PIPER said: > [quoted text clipped - 11 lines] > May I suggest at this point that you'd be more swiftly satisfied by asking > about the requisite number of angels for pinhead-dancing? Mike-UK - 03 Mar 2005 01:13 GMT http://tinyurl.com/6vwgs
may be of some use to you.
Enjoy. :)
> Ah I see, so he isnt legit. Got it, thanks.This kind of caught my eye since > i have heard about taurine for blood sugar control. [quoted text clipped - 16 lines] > > May I suggest at this point that you'd be more swiftly satisfied by asking > > about the requisite number of angels for pinhead-dancing?
 Signature --------------------------- http://tinyurl.com/4872c
Have a nice day, it really does do you good! :)
Michael Baugh - 01 Mar 2005 11:02 GMT What a wonderfully apt analogy.
> May I suggest at this point that you'd be more swiftly satisfied by asking > about the requisite number of angels for pinhead-dancing? Johan - 01 Mar 2005 13:35 GMT > What a wonderfully apt analogy. Michael's very good at that.:)
> > May I suggest at this point that you'd be more swiftly satisfied by asking > > about the requisite number of angels for pinhead-dancing? The answer to that question depends on whether they're doing a Pavane, Minuet, Waltz, Tango, Fox Trot, Morris Dancing, Square Dancing, or plain Two-Step.
Texas Line-Dancing even angels wonder about. (It's a favorite debate question at the Bi-Millenial All-Universe Solipsism Tournament. i.e Resolved: No more than "X" angels can line-dance upon the head of a pin.)
Interpretive Dance often requires only one.
Deirdre - 02 Mar 2005 00:47 GMT <Line-Dancing even angels wonder about. (It's a favorite debate question at the Bi-Millenial All-Universe Solipsism Tournament. i.e Resolved: No more than "X" angels can line-dance upon the head of a pin.) Interpretive Dance often requires only one.>
Okay. Interpretive dance, hum. Let me me. How about someone actually went through the drill (*again*) to make sure everything still works on the cellular (pin-point) level - as previously determined many, many times? just in case people don't believe that life happens at all - and especially just in case people think substances are "dangerous" and want to ban 'em all?
Actually it's quite interesting to see everything still works and that someone can actually get away with a re-affirmation and - somehow - scrape together the $$ to do it, submit the thing for PR and someone will actually publish it?
I thnk it's kinda sweet. Something like "Songs from Pippa Passes". "God's in his / her heaven, all's right / wrong with the world." IOW it's okay.
I'm really impressed that someone actually used the word "solipsism". Crashing reminder of the unused vocabultary - what's the incidence of this word in common use? I hope it's still in the Oxford.
You have to ujnderstand the Thomas. He is - or used to be - a vegetarian. If I've had any influence at all he is at least wavering and considering his patriotic duty to consume Alberta beef in large quantities. Of if he can get it, British Columbia beef is even better. Anyway I owe the Thomas (aka the Clanking One - a vote of thanks since I finally got my serum ferritin done just to rule out hemochromatosis which was the one remote possibility that iron would cause any problems whateoever. Anyway as it transpired last fall, so far was I from a case of hemochromatosis that I have an IRON DEFICIENCY ANEMIA - and that's a no brainer considering I have Crohn's disease so what else would have been new? so now I take my iron tablets and am begining - SLOWLY - to feel MUCH better and - ta DA - my BRAIN is much less FOGGY.
I'm now willing to attribute Fibrofog to IDA and say "get your serum ferritin checked" because ta the same brood draw I had a NORMAL CBC (of course "normal" is just ridiculous) but hemoglobin "fine" (according to doctor) and no microcytic anemia - so how would he ever have known I had an IDA if I had not asked for a serum ferritin, and I never would have asked for it if I hadn't been reading the Clanking One's posts for the past six years on and off - but of course he's been posting about the supposed evils of iron supplementation and the eating of red meat - so thereyago. It's an important point and he raised it. It only took me SIX years to get the test done and to act on the results and start treating this HORRIBLE deficiency disease. Which has NOTHING to do with diet.
So not to worry about the Clanking One - we just have to keep telling each other "don't forget to get your serum ferritin and your serum vitamin D done" and gradually, gee, maybe after a few years we wouldn't have much of a newsgroup left. Except no, I don't think so. I think we're always going to have some people here trying to find out how they can get their body/brains back - I'm just beginning to get mine back and one of my first thoughts - after giving all the appropriate thanks - including thanks to the Clanking One for keeping this debate alive and up here (no matter how inadvertant that might have been) - I am planning my Next Move which is to go back and finish what I started a Long Time Ago. ;-)))
Cheers from Mrs Magoo who gives credit where credit is due (~Q'Q~)
> > What a wonderfully apt analogy. > [quoted text clipped - 12 lines] > > Interpretive Dance often requires only one. Chip Orange - 02 Mar 2005 03:43 GMT And neither do you, but that doesn't stop you from spewing forth every time he posts an abstract of a paper. It really is more annoying than his abstracts.
> PIPER said: > [quoted text clipped - 11 lines] > May I suggest at this point that you'd be more swiftly satisfied by asking > about the requisite number of angels for pinhead-dancing? Michael - 02 Mar 2005 03:59 GMT Chip Orange said:
> And neither do you, Ah, but I do. Not everything, or fully - I'm not trained at all in the field - but I do have the help of a molecular biologist for clarifying things when I'm stuck.
> but that doesn't stop you from spewing forth > every time he posts an abstract of a paper. It really is more > annoying than his abstracts. I'm sorry you feel that way.
((U)) M
>> PIPER said: >> [quoted text clipped - 11 lines] >> May I suggest at this point that you'd be more swiftly satisfied by >> asking about the requisite number of angels for pinhead-dancing? Dave ???? - 02 Mar 2005 16:36 GMT Howdy!
The orthadox answer to "How many angels can dance on the head of a pin?" is:
AS MANY AS WANT TO!
 Signature Dave ????
http://www.howdydave.com
> > PIPER said: [quoted text clipped - 12 lines] > May I suggest at this point that you'd be more swiftly satisfied by asking > about the requisite number of angels for pinhead-dancing? rose - 02 Mar 2005 16:41 GMT Dave ©¿©¬ wrote:
> Howdy! back atcha, dave! ;->
> The orthadox answer to "How many angels can dance on the head of a pin?" is: > > AS MANY AS WANT TO! hmmm...i'd heard that the 'proper' answer is ZERO -- because angels are non-corporeal beings, they would not dance 'on' anything, be it pin or dance floor! ;-> rose
Deirdre - 03 Mar 2005 08:43 GMT > Dave =A9=BF=A9=AC wrote: > > Howdy! [quoted text clipped - 9 lines] > non-corporeal beings, they would not dance 'on' anything, be it pin or > dance floor! ;->
> rose Howdy. What a pleasure meeting y'all here. I'd like to take up the matter of the nature of angels.
I believe Dante established their corporeal nature in "The Inferno", when he and his friend ascended from the horrors of the Flaming Pit.
I heard Dante's translator (or I guess one of them, this one is current) describing how the corporeal - non corporeal thing was not really documented with any definition before Dante wrote his "Inferno" - it seems the most impressive aspect of his creative genius lay in his actually described in terms generally used of a real corporeal existence every imaginable detail of the Infernal Regions.
And most tellingly, he described his ascent from the Inferno which he and his friend accmplished by clambering with considerable difficulty on and over and up the clearly corporeal body of Satan. It was my understanding from that discussion that Dente had more or less cast the various technical details in A Form. Till his day anyone could have any idea of the details of Paradise and Hell and the terrestrial world. I suppose Dante would have been working along lines similar to that of a natural historian. It seems Dante thought the terrestrial world the most beautiful of all the spheres of existence.
It's interesting that someone let this matter slither away here to examine the matter of interpretive dance which was done by one. ;-)
Mrs Magoo (~Q'Q~)
Dave ???? - 05 Mar 2005 17:31 GMT > > Dave =A9=BF=A9=AC wrote: > > > Howdy! [quoted text clipped - 39 lines] > > Mrs Magoo (~Q'Q~) Howdy Mrs. M!
Sorry to say... Dante didn't establish anything! He only made some speculations.
As with any other speculation that can neither be confirmed nor denied by physical evidence, in the end their existance is all a matter of personal faith.
His interpretation of the spheres of existance is a matter of cultural upbringing. You would get a quite different point of view if Dante had lived in India, China, South America or aboriginal Australia.
 Signature Dave ????
http://www.howdydave.com
ironjustice@aol.com - 05 Mar 2005 19:26 GMT >>Some of this I can understand but not all, could you put in layman<< Taurine is .. good ..
Taurine does not allow the bad things which happen because of .. rust ..
Who loves ya. Tom Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore! http://pages.ivillage.com/ironjustice/manisaherbivore DEAD PEOPLE WALKING http://pages.ivillage.com/ironjustice/deadpeoplewalking
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