Slightly more detailed reply:

Adv Dent Res. 1998 Nov;12(2):32-9. Related Articles, Links  

Long-term sub-antimicrobial doxycycline (Periostat) as adjunctiv
management in adult periodontitis: effects on subgingival bacteria
population dynamics.

Thomas JG, Metheny RJ, Karakiozis JM, Wetzel JM, Crout RJ.

Department of Pathology, West Virginia University, Morgantow
26506-9203, USA.

Previous trials had indicated that various schedules o
sub-antimicrobial doxycycline significantly reduced gingival crevicula
fluid (GCF) collagenase activity in adult patients with periodontiti
with no evidence of emergent tetracycline-resistant (Tcr) marker ora
flora. The purpose of this nine-month study was to expand thes
observations, emphasizing newer microbial diagnostic methods
Subgingival paper point samples were obtained at baseline (BL), 3, 6
and 9 months. Four subject treatment groups in a double-blind desig
were evaluated by mechanical scaling and root planing (SRP) and/or 2
mg doxycycline BID (Periostat). Thirty-eight patients entered the stud
at baseline (BL). Dark-field microscopy on 260 samples showed tha
morphotype distribution was independent of treatment schedule. Cultur
analysis of the 3 most prevalent isolates recovered showed tha
Streptococcus and Prevotella species accounted for approximately 85% o
the 724 cultures. There did not appear to be any overgrowth o
replacement by opportunistic oral flora. Of 658 susceptibility pattern
evaluated by Etest, the MIC50/90 and mode MIC showed stable patterns
independent of treatment group. Our findings were different from thos
of previously published reports, but may be partly explained by th
lack of universally standardized methods in oral microbiology an
interpretive criteria for susceptibility testing.

Publication Types:
Clinical Trial
Randomized Controlled Tria

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Joel34