Int J Oncol. 2005 Jan;26(1):241-6. Related Articles, Links

In vivo and in vitro effect of baicalein on human prostate cancer
cells.

Miocinovic R, McCabe NP, Keck RW, Jankun J, Hampton JA, Selman SH.

Urology Research Center, Department of Urology, Medical College of
Ohio, Toledo, OH 43614, USA.

We investigated the in vitro effects of baicalein and baicalin on human
umbilical vein endothelial cells (HUVECs) and on human prostate tumor
cells (DU-145 and PC3) as well as the effect of orally administered
baicalein on the growth of DU-145 cells after subcutaneous injection
into SCID mice. In vitro effects of baicalein and baicalin treatment on
human prostate cancer cell lines DU-145 and PC-3 were assessed by
employing cell proliferation (MTS) assay, cytotoxicity (LIVE/DEAD)
assay, and TUNEL assay. In vitro anti-proliferative and anti-angiogenic
properties of baicalein and baicalin were studied on HUVECs by sprout
assay. The effect of orally administered baicalein on tumor growth in
SCID mice was studied in four groups (n=10) of animals injected
subcutaneously with DU-145 cells and treated daily for 28 days. The
control group received only vehicle (carboxymethylcellulose), whereas
the other three groups received escalating doses of baicalein (10, 20,
and 40 mg/kg per day). Baicalein and baicalin exhibit dose-dependent
growth inhibitory effects on human prostate cancer cells and umbilical
vein endothelial cells in vitro. Also, treatment by these two flavonoid
compounds significantly decreased the average number and length of
sprouts formed by the endothelial cell aggregates in a dose-dependent
manner. In vivo, treatment of mice with baicalein demonstrated a
statistically significant tumor volume reduction (p<0.01) when compared
to the control. This is the first study demonstrating an in vivo growth
inhibitory effect of orally administered baicalein on human prostate
tumors in mice.

PMID: 15586246 [PubMed - in process]

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Biochem Mol Biol Int. 1996 May;39(2):215-25. Related Articles, Links

Inhibition of microsomal lipid peroxidation by baicalein: a possible
formation of an iron-baicalein complex.

Gao D, Sakurai K, Katoh M, Chen J, Ogiso T.

Shenyang College of Pharmacy, P.R. China.

Baicalein decreased the production of thiobarbituric acid reactive
substances, the rate of oxygen consumption and iron reduction in the
reaction system of ascorbic acid with FeCl3. Superoxide dismutase,
catalase and hydroxyl radical scavengers had no significant effect.
Iron-chelators had an inhibitory effect similar to that of baicalein.
The production of thiobarbituric acid reactive substances of
baicalein-treated microsomes obtained by centrifugation after
incubation with baicalein was not observed in the reaction system, but
was stimulated by adding iron with increases in concentration. The
amount of bound iron to microsomal membranes increased by increasing
both the concentration of baicalein and iron. The amount of baicalein
bound to microsomal membranes increased with increasing concentration
of added baicalein. These results suggest that baicalein bound to
microsomal membranes inhibits lipid peroxidation by formating an
iron-baicalein complex.

PMID: 8799447 [PubMed - indexed for MEDLINE]

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