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Medical Forum / Diseases and Disorders / Arthritis / October 2005Toxic-free iron in the joint / rheumatoid arthritis
Poster presentation Antibodies to ferritin in rheumatoid arthritis are associated with disease severity D Mewar1, DJ Moore2, PF Watson3 and AG Wilson1 1Division of Genomic Medicine, University of Sheffield, UK 2Department of Radiology, Royal Hallamshire Hospital, Sheffield, UK 3Division of Clinical Sciences North, University of Sheffield, UK from 25th European Workshop for Rheumatology Research Glasgow, UK, 24-27 February 2005 Arthritis Research & Therapy 2005, 7(Suppl 1):P128 doi:10.1186/ar1649 Received 11 January 2005 Published 17 February 2005 © 2005 BioMed Central Ltd -------------------------------------------------------------------------------- Background and objectives A number of autoantibodies have been described in individuals with rheumatoid arthritis (RA) leading to interest in their use as diagnostic or prognostic markers in RA as well as their pathogenic relevance. By immunoscreening of a phage-display expression cloning system with RA patient sera we isolated a cDNA clone encoding the ferritin H chain polypeptide. The objectives of this study were to establish the frequency and clinical associations of anti-ferritin antibodies in RA. Methods We employed an ELISA for the measurement of anti-ferritin antibodies in RA sera. Briefly, 96-well plates were coated overnight with purified ferritin from equine spleen. Sera diluted one in 100 were reacted with the plates for 16 hours at 4°C. After three washes, a goat anti-human IgG/horseradish peroxidase conjugate was used to detect bound IgG. The signal for each serum sample was normalised using a reference serum included on each plate. Anti-ferritin antibodies were measured in 291 subjects with RA, 73 healthy blood donors and 91 subjects with osteoarthritis. Antibody-positive and antibody-negative individuals were compared with respect to severity of disease as measured by modified Larsen's score, demographic variables, rheumatoid factor status and carriage of HLA DRB1 shared epitope alleles. Correlations were examined between antibody levels and severity of joint damage assessed by the Modified Larsen's score. Results Using a cut-off index of three standard deviations above the mean of the control group, 49/291 (16.8%) RA patients were positive versus 2/73 (2.7%) healthy donors and 2/91 subjects with osteoarthritis (P < 0.01). In six positive and six negative sera these findings were confirmed by western blotting. Anti-ferritin antibodies were more common in males with RA (25.3% males versus 13.7% females, P < 0.02) and levels were positively associated with severity of joint damage (rs = 0.33, P = 0.02). Conclusions Anti-ferritin antibodies are present in a subset of individuals with RA and are associated with more severe joint damage. Ferritin is an abundant protein in serum and synovial fluid, and therefore ferritin/anti-ferritin immune complexes could form in these individuals and contribute to joint damage. An alternative possibility is that anti-ferritin antibodies could modulate the iron-binding properties of ferritin and so lead to the release of toxic-free iron in the joint. We are currently examining the frequency of anti-ferritin reactivity in patients with early RA at two timepoints in order to establish whether or not these antibodies are present in early disease or are simply a result of longstanding disease, as well as their frequency in other autoimmune diseases. These data will be presented. Who loves ya. Tom Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore! http://pages.ivillage.com/ironjustice/manisaherbivore DEAD PEOPLE WALKING http://pages.ivillage.com/ironjustice/deadpeoplewalking Tommah, you really are a complete fucktard. The study was about antibodies to ferritin causing it to release the bound iron. It's the antibodies, not the iron, that are the problem: no auto-antibodies = no iron unbinding. But I guess a high school drouput like you can't really be blamed for having no critical thinking skills. >>The study was about antibodies to ferritin causing it to release the bound iron<< Free iron in the joint causes destruction of .. joint .. PROVEN .. The article speaks to .. free iron .. in .. joint .. Now .. it don't take no rocket scientist .. But it DOES take .. a .. teenyweenybitof .. intelligence .. Obviously .. evidence based .. YOU .. lack even the .. 'teenyweenybit' .. Heh .. heh .. Who loves ya. Tom Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore! http://pages.ivillage.com/ironjustice/manisaherbivore DEAD PEOPLE WALKING http://pages.ivillage.com/ironjustice/deadpeoplewalking >>>The study was about > [quoted text clipped - 3 lines] > > PROVEN .. If you'd have take one high school science class you'd know that things are supported or not supported, but not "proven". That word is a no-no in science. Then again, if you'd passed any classes after grade six you'd have been able to punctuate properly instead of typing periods (full stops for those of us in Commonwealth nations) over and over again. You were just too lazy, and it shows! Not to mention the fact that you fail to grasp what was printed quite clearly in that abstract (clearly for those of us with even rudimentary english comprehension skills): the antibodies cause the release of iron from the ferritin-iron complex. The antibodies are why the iron is 'free'. No antibodies, no free iron and no problem. Have a nice day Tommah, try not to touch yourself until you've let the sores heal. the antibodies cause the release of iron from the ferritin-iron complex. The antibodies are why the iron is 'free' << The iron is .. free .. The painkiller .. aspirin .. binds .. coincidentally .. iron .. YOU ..'say' .. iron is NOT .. involved .. ? Free iron is NOT .. involved .. ? Article says it .. is .. Studies .. have .. PROVEN .. IRON .. DESTROYS .. JOINTS .. "teenyweenybit' .. at the VERY .. least .. Heh .. heh .. Who loves ya. Tom Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore! http://pages.ivillage.com/ironjustice/manisaherbivore DEAD PEOPLE WALKING http://pages.ivillage.com/ironjustice/deadpeoplewalking > the antibodies cause the release of iron from the ferritin-iron > complex. The antibodies are why the iron is 'free' > << > > The iron is .. free .. Only because auto-antibodies mess up the ferritin-iron complex, 'tard boy! I guess your compulsive masturbation has had a negative effect on your sight, because you don't appear to be able to read all the words anymore. Then again, it could just be the syphilis rotting your brain. Either way, get help. Oh that's right, you're too afraid, because whenever you do they put you in restraints and haul you away. > The iron is .. free .. Only because auto-antibodies mess up the ferritin-iron complex<< Oh .. I .. seeeeeeee .. You are arguing .. HOW .. the iron got into the joint .. You understand and accept the iron IS causing .. damage .. I .. seeeee ... So .. what seems to be your .. problem .. with .. my .. post .. ? The article says the .. free iron .. is most likely .. causing .. pain and damage .. You seem to have a problem with me posting that .. type .. of medical study .. ? Eat .. me .. Who loves ya. Tom Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore! http://pages.ivillage.com/ironjustice/manisaherbivore DEAD PEOPLE WALKING http://pages.ivillage.com/ironjustice/deadpeoplewalking Tommah, without iron you'll die. In fact, I think that's a good plan. You should keep giving blood until you run out of iron. Come back and post to let us know how it goes when you have a ferritin of zero. If you're not willing to practice what you preach, then STFU, bitch :-) 2005-10-06, Responding to spodosaurus... > Tommah, without iron you'll die. In fact, I think that's a good plan. > You should keep giving blood until you run out of iron. Come back and > post to let us know how it goes when you have a ferritin of zero. If > you're not willing to practice what you preach, then STFU, bitch :-) Hot damn! Spod, when you're good, you're GOOD! 8)  Signature---- * Another squeaking wheel @ http://tinyurl.com/6bf56 * Mike's (curious) Brain @ http://tinyurl.com/4872c - Have a nice day, it really does do you good! :) > 2005-10-06, Responding to spodosaurus... > [quoted text clipped - 4 lines] > > Hot damn! Spod, when you're good, you're GOOD! 8) I was TRYING to be BAD :P > > 2005-10-06, Responding to spodosaurus... > > [quoted text clipped - 6 lines] > > I was TRYING to be BAD :P And we thank you for it. SignatureAF 2005-10-06, Responding to spodosaurus... >> 2005-10-06, Responding to spodosaurus... >> [quoted text clipped - 6 lines] > > I was TRYING to be BAD :P And damn good at it you are to be sure! ;\ Signature---- * Another squeaking wheel @ http://tinyurl.com/6bf56 * Mike's (curious) Brain @ http://tinyurl.com/4872c - Have a nice day, it really does do you good! :) >Have a nice day Tommah, try not to touch yourself until you've let the >sores heal. Beautiful! I love it. Thanks for the laugh, Ari. --- Joan i keep thinking . . . this guy must be stuck in some kind of time warp--- like when he stepped on a rusty nail that traumatized him; and he is still blaming that nail for all his life's woes. kate ah well, maybe his stuck life does serve a purpose for others---a target. sure wish he'd get unstuck, though. On Wed, 05 Oct 2005 22:06:18 +0800, spodosaurus <spodosaurus@_yahoo_.com> wrote in alt.support.arthritis: >Have a nice day Tommah, try not to touch yourself until you've let the >sores heal. Beautiful! I love it. Thanks for the laugh, Ari. --- Joan 2005-10-06, Responding to d'huit... > i keep thinking . . . this guy must be stuck in some kind > of time warp--- like when he stepped on a rusty nail that > traumatized him; and he is still blaming that nail for all > his life's woes. Splut! Monitor cleaning now in progress... Good one! :) Signature---- * Another squeaking wheel @ http://tinyurl.com/6bf56 * Mike's (curious) Brain @ http://tinyurl.com/4872c - Have a nice day, it really does do you good! :) >Now .. it don't take no rocket scientist .. Oh, Tom-Tom........ your grammar! Tut, tut. --- Joan Rusty (Tom/Iron whos-a-ma-thingy) has no connnection to the real world. All of his posts are "off topic" because he doesn't know what a topic is. For your own health and well-being, ignore his "advice". > Rusty (Tom/Iron whos-a-ma-thingy) has no connnection to the real world. > > All of his posts are "off topic" because he doesn't know what a topic > is. > > For your own health and well-being, ignore his "advice". Hi Bones,,,, Long time no see. How about a little catch up on your world.?.?.? Harv It's been one of those... I was nursing my computer along until it finally would only run in safe mode (computer crashed while I was working on the registry, and then it was really sick). I got a new computer with really great specs for what I wanted to do, got it set up and most of the programs installed when it wouldn't boot. I called the store and brought it in (I don't do my own repairs if it's under warranty). They gave me a replacement (it was still in the first 30 days). I (again) installed most of the programs and the same thing happened. I called the store VERY upset and was told that there appeared to be a problem with the motherboard design and that I was no the only one having problems. They gave me a new computer (Different make and model) and I'm still setting this one up. I'm also still taking care of my father (he has Parkinson's) and dealing with my own problems. I think that if I woke up one day and nothing was wrong I'd start to worry. > iron. iron. iron. Look fella, iron isn't that important. Who cares. One day your'e going to look back and say: "I can't believe I wasted all that time thinking about stupid iron." 2005-10-05, Responding to zooplankton... >> iron. iron. iron. > > Look fella, iron isn't that important. Who cares. One day your'e going > to look back and say: "I can't believe I wasted all that time thinking > about stupid iron." Ya think maybe? http://tinyurl.com/4thvq Not this week I don't think. ;\ Signature---- * Another squeaking wheel @ http://tinyurl.com/6bf56 * Mike's (curious) Brain @ http://tinyurl.com/4872c - Have a nice day, it really does do you good! :) >> iron. iron. iron. > > Look fella, iron isn't that important. Who cares. One day your'e going > to look back and say: "I can't believe I wasted all that time thinking > about stupid iron." Yup - much more likely to die of crossposting than rust. Trust me. >>I tell you what .. Look fella, iron isn't that important. Who cares. One day your'e going to look back and say: "I can't believe I wasted all that time thinking about stupid iron." << INDOCIN (INDOMETHACIN) http://www.headaches.org/consumer/topicsheets/indocin.html Indomethacin (Indocin) is an analgesic. It is classified as a non-steroidal antiinflammatory drug. These are commonly referred to as NSAIDS. Other NSAIDS are also used as analgesics. Indomethacin can be used as a pain reliever or analgesic. It can also be used as a treatment for arthritis, fever or other conditions where inflammation plays a role in creating the symptoms. Unlike steroids, the side effects with Indomethacin are not as likely to be common or severe. The most common side effects include stomach upset, dizziness and tiredness. It may make stomach ulcers worse. It can have effects on kidney and liver function when used chronically. Studies with this agent have demonstrated it to be effective in several forms of cluster headache known as atypical cluster headache and chronic paroxysmal hemicrania. It is the medication of first choice in these two forms of cluster headache. Indomethacin may also be helpful in headache related to physical stress or exertional headache. It has been shown to be effective in migraine both for prevention of migraine as well as treating the acute migraine attack. ------------------------------?------------------------------?--------- Metab Brain Dis 2003 Mar;18(1):1-9 Indomethacin reduces lipid peroxidation in rat brain homogenate by binding Fe2+. Anoopkumar-Dukie S, Lack B, McPhail K, Nyokong T, Lambat Z, Maharaj D, Daya S Faculty of Pharmacy, Rhodes University, Grahamstown, South Africa. [Medline record in process] One of the hallmarks of Alzheimer's disease (AD) is the progressive degeneration of cholinergic neurons in the cerebral cortex and hippocampus. It is generally accepted that this neuronal degeneration is due to free-radical-induced damage. These free radicals attack vital structural components of the neurons. This implies that agents that reduce free radical generation could potentially delay the progression of AD. Free radical generation in the brain is assisted by the presence of iron, required by the Fenton reaction. Thus, agents that reduce iron availability for this reaction could potentially reduce free radical formation. Since non steroidal anti-inflammatory drugs (NSAIDS) have been shown to reduce the severity of AD, we investigated the possible mechanism by which indomethacin could afford neuroprotection. Our results show that indomethacin (1 mM) is able to reduce the iron-induced rise in lipid peroxidation in rat brain homogenates. In addition, our NMR data indicate that indomethacin binds the Fe(2+)/Fe(3+) ion. This was confirmed by a study using UV/Vis spectrophotometry. The results imply that indomethacin provides a neuroprotective effect by binding to iron and thus making it unavailable for free radical production. PMID: 12603077, UI: 22490682 Ann Rheum Dis 1989 May;48(5):382-8 Investigation of the anti-inflammatory properties of hydroxypyridinones. Hewitt SD, Hider RC, Sarpong P, Morris CJ, Blake DR Cancer Research Unit, University of York, Heslington. Synovial iron deposition associated with rheumatoid disease may result in the production of highly reactive oxygen free radicals, leading to tissue damage. This chain of events can be interrupted by iron chelation. Families of strong iron (III) chelators have been tested for their iron scavenging properties in vitro and their effects assessed in vivo using a rat model of inflammation. All the chelators competed successfully for iron with apotransferrin, and some removed up to 34% of iron from ferritin. The best anti-inflammatory effects were achieved with the most hydrophilic chelators and those which chelated iron most avidly. Activity was dependent on dose. The route of administration was also an important factor with lower affinity chelators. This work introduces a range of simple bidentate iron chelators, which under certain conditions exceed desferrioxamine in their iron scavenging abilities, and some of which, in this simple animal model, approach indomethacin in their anti-inflammatory capabilities. PMID: 2730166, UI: 89272259 Med Hypotheses 1998 Mar;50(3):239-51 A chelate theory for the mechanism of action of aspirin-like drugs. Wang X Department of Pathology, Cornell University Medical College, New York, NY 10021, USA. x...@mail.med.cornell.edu Two hundred years after the discovery of the pharmaceutical usefulness of aspirin, it and aspirin-like drugs, a family with an ever-increasing number of members, are an indispensable part of modern life. However, the question as to how these drugs work in the body has remained unsettled. It is postulated here that this group of drugs may exert their therapeutic (and adverse) effects by chelating various physiologically important metallic cations in the body. The chelate theory is supported by the vast majority, if not all, of the observations on these drugs made in the past. Publication Types: * Review * Review, academic PMID: 9578329, UI: 98237440 _________________________________________________________________ Who loves ya. Tom Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore! http://pages.ivillage.com/ironjustice/manisaherbivore DEAD PEOPLE WALKING http://pages.ivillage.com/ironjustice/deadpeoplewalking << |
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