Cell Biochem Funct. 2005 Sep 2; [Epub ahead of print] Links

Investigation of protein oxidation and lipid peroxidation in patients
with rheumatoid arthritis.

Baskol G, Demir H, Baskol M, Kilic E, Ates F, Karakukcu C, Ustdal M.

Department of Biochemistry and Clinical Biochemistry, Erciyes
University, Faculty of Medicine, Kayseri, Turkey.

We aimed to determine the importance of neutrophil activation and the
source of oxidative stress in the pathogenesis of rheumatoid arthritis
(RA) by quantification of advanced oxidation protein products (AOPP)
and total thiol levels as markers of oxidative protein damage,
malondialdehyde (MDA) levels as a marker of lipid peroxidation and
myeloperoxidase (MPO) activity as a marker of neutrophil activation in
patients with RA. Fifty-seven rheumatoid arthritis patients were
included in the study and sub-grouped according to disease activity
(active, n = 31; inactive, n = 26) and compared with healthy controls
(n = 25). Serum MPO activity, AOPP, MDA, and thiol levels were measured
by an enzymic spectrophotometric method. Serum MPO activity (p <
0.001), AOPP (p < 0.001), MDA (p < 0.001) and levels of thiol (p <
0.002), were higher in the patient group than the controls. Active and
inactive RA groups were compared with the control group and there were
significant differences between each parameter. MPO activity, AOPP, MDA
and thiol levels were significantly higher in both active and inactive
RA patients than the controls. On the other hand, when a comparison was
made between active and the inactive stage, a statistically significant
difference was present only in MDA (p < 0.05) and AOPP levels (p <
0.05). There was also a significant positive correlation between all
parameters. These data strongly suggest that neutrophils, which
constitute the most important source of chlorinated oxidants due to
their high MPO content, may be involved in serum AOPP formation and
therefore the production of a novel class of pro-inflammatory mediators
of oxidative stress in RA patients and that protein oxidation could
play an important role in the pathogenesis of RA as does lipid
peroxidation. Copyright (c) 2005 John Wiley & Sons, Ltd.

PMID: 16142689 [PubMed - as supplied by publisher]

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