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Medical Forum / Diseases and Disorders / Arthritis / May 2005Glycosaminoglycans limit oxidative damage
Toxicol In Vitro. 2005 Aug;19(5):561-572. Epub 2005 Apr 22. Related Articles, Links Purified human plasma glycosaminoglycans limit oxidative injury induced by iron plus ascorbate in skin fibroblast cultures. Campo GM, Avenoso A, D'Ascola A, Campo S, Ferlazzo AM, Sama D, Calatroni A. Department of Biochemical, Physiological and Nutritional Sciences, School of Medicine, University of Messina, Policlinico Universitario, Torre Biologica, 5 degrees piano, Via C. Valeria 98125, Messina, Italy. A number of studies in vivo and in vitro showed that high levels of glycosaminoglycans (GAGs) are found as a consequence of free radical damage. The GAG over production may represent an endogenous mechanism capable to limit oxidative damage. Based on these hypotheses, the aim of this study was to evaluate the antioxidant property of GAGs of human origin in fibroblast cultures. Purified human plasma GAGs were added to the fibroblast cultures in which oxidative stress was induced by the oxidizing system employing iron (Fe(2+)) plus ascorbate. We assessed cell death, lactate dehydrogenase activity, membrane lipid peroxidation, DNA damage, protein oxidation, hydroxyl radical (OH()) generation and endogenous antioxidant depletion. The exposure of fibroblasts to FeSO(4) produced cell death and increased OH() production. It also caused DNA strand breaks and protein oxidation as shown by the DNA fragment analysis and protein carbonyl content, respectively. In addition, FeSO(4) enhanced lactate dehydrogenase activity and lipid peroxidation while decreased antioxidant defences. Purified human GAGs, at three different doses, reduced cell death, limited DNA fragmentation and protein oxidation, decreased OH() generation and lactate dehydrogenase activity, inhibited lipid peroxidation and improved endogenous antioxidant defences. These results further support the hypothesis that these molecules may function as antioxidants. PMID: 15896549 [PubMed - as supplied by publisher] -------------------------------------------------------------------------------- Who loves ya. Tom Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore! http://pages.ivillage.com/ironjustice/manisaherbivore DEAD PEOPLE WALKING http://pages.ivillage.com/ironjustice/deadpeoplewalking This poster has an anal fixation on IRON. He feels that it is the cause of every human ailment known to mankind. > This poster has an anal fixation on IRON. > > He feels that it is the cause of every human ailment known to mankind. I think he ate LEAD-based paint on his crib when he was an infant. And he's still behaving as an infant. ... A nude Buddhist practices yaga bare. > ... A nude Buddhist practices yaga bare. Would a transcendentalist radio amateur transmit with a yogi yagi? > I think he ate LEAD-based paint on his crib when he was an infant. > > And he's still behaving as an infant. Nah, Chief, most infants are cute and cuddly at least part of the time. That doesn't EVER apply in this case. :-) --- Joan And .. remember .. aspirin is an .. iron .. binder .. Norman is of the 'persuasion' .. aspirin is NOT .. used for .. pain .. Newman .. is .. obviously .. stupid .. Aspirin IS .. used for pain .. Contrary to Normans' .. "aspirin is NOT used for ..pain" .. Again .. let me reiterate for those who are handicapped .. Aspirin IS used for .. pain .. Understand .. ? Heh .. heh .. Who loves ya. Tom Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore! http://pages.ivillage.com/ironjustice/manisaherbivore DEAD PEOPLE WALKING http://pages.ivillage.com/ironjustice/deadpeoplewalking >Again .. let me reiterate for those who are handicapped .. The only handicapped person I see here is you. So why not take a bottle of aspirin to help ease YOUR pain? --- Joan > And .. remember .. aspirin is an .. iron .. binder .. > [quoted text clipped - 11 lines] > > Understand .. ? Maybe it would be easier if you were able to type messages in english instead of like a lazy shut in version of Captain Kirk?  Signaturespammage trappage: replace fishies_ with yahoo I'm going to die rather sooner than I'd like. I tried to protect my neighbours from crime, and became the victim of it. Complications in hospital following this resulted in a serious illness. I now need a bone marrow transplant. Many people around the world are waiting for a marrow transplant, too. Please volunteer to be a marrow donor: http://www.abmdr.org.au/ http://www.marrow.org/ IronJustice doesn't write the studies. He just posts them. They're out there; someone is FUNDING them; maybe you should pay attention. Zee > This poster has an anal fixation on IRON. > > He feels that it is the cause of every human ailment known to mankind. >IronJustice doesn't write the studies. He just posts them. They're out >there; someone is FUNDING them; maybe you should pay attention. > >Zee But he posts anything with a key word in it that fits his pet version of things at the moment. He doesn't understand what he posts. A lot of published 'medical studies' do not stand up to examination. --- Joan And he has some sort of program that searches for the word Iron. Even if you are talking about buying a iron for pressing shirts, he pips in with how he can fix that. Never actually reads anyone's posts or he would know that we were not discussing his favorite topic. Some of those research he quotes he has bent and snipped to fit his mood. Some of the research is flawed in that there was never a double blind. He has been posting this crap for years and is known only for doing it. Never for support and never for anything else. And unless he has changed his web site, it was holding a nasty virus several years ago. I suggest you NOT go there and check unless you are thoroughly protected against trouble. Lots of us have been around here for years and this guy has a record in Google of his repetitious nonsense. Duckie [who wants Newbies to know we post this stuff to try and protect you from the nonsense. That includes noni juice, blue stuff, emu oil, and a bunch of other junk that Dr. Doc always says 'Watch your Wallet'. At least Ironboy is not selling anything but free words] >>IronJustice doesn't write the studies. He just posts them. They're out >>there; someone is FUNDING them; maybe you should pay attention. [quoted text clipped - 7 lines] > --- > Joan Signature_('> (_<_) _ _('< -quack (_<_) _ __('< *QUACK!* <_{__) _('< "|,,|_" (_<_) _('< "AFLAC!" (_<_) > IronJustice doesn't write the studies. He just posts them. They're out > there; someone is FUNDING them; maybe you should pay attention. > > Zee LOLOL Welcome to ASA Zee where people do pay attention and know BULLSHIT when we read it and the spreading around does not change the smell. LOLOL Harv > IronJustice doesn't write the studies. He just posts them. They're out > there; someone is FUNDING them; maybe you should pay attention. > > Zee Anyone can cut and paste an abstract. Tommy boy doesn't have the education or the grey matter to comprehend what he's reading. He just searches for keywords, copy and pastes to spam multiple newsgroups, and then goes back to masturbating furiously (he also tries to recruit other people into his pseudo-christian cult, because he's lonely all by himself and the FBI keep ignoring him). >>This poster has an anal fixation on IRON. >> >>He feels that it is the cause of every human ailment known to > > mankind. Signaturespammage trappage: replace fishies_ with yahoo I'm going to die rather sooner than I'd like. I tried to protect my neighbours from crime, and became the victim of it. Complications in hospital following this resulted in a serious illness. I now need a bone marrow transplant. Many people around the world are waiting for a marrow transplant, too. Please volunteer to be a marrow donor: http://www.abmdr.org.au/ http://www.marrow.org/ |
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