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Medical Forum / Diseases and Disorders / Arthritis / October 2007Arthritis and Fullerene
A few years ago I discussed in length with a man about the **need** for an efficient safe iron chelator and whether his work with the buckyball could be somehow developed into an iron chelator .. Voila .. ? http://www.bristol.ac.uk/Depts/Chemistry/MOTM/buckyball/c60a.htm Arthritis Rheum. 2007 Sep 28;56(10):3307-3318 [Epub ahead of print] Links Water-soluble C60 fullerene prevents degeneration of articular cartilage in osteoarthritis via down-regulation of chondrocyte catabolic activity and inhibition of cartilage degeneration during disease development. Yudoh K, Shishido K, Murayama H, Yano M, Matsubayashi K, Takada H, Nakamura H, Masuko K, Kato T, Nishioka K. St. Marianna University School of Medicine, Kawasaki, Japan. OBJECTIVE: Studies have shown the roles of oxidative stress in the pathogenesis of osteoarthritis (OA) and induction of chondrocyte senescence during OA progression. The aim of this study was to examine the potential of a strong free-radical scavenger, water-soluble fullerene (C60), as a protective agent against catabolic stress-induced degeneration of articular cartilage in OA, both in vitro and in vivo. METHODS: In the presence or absence of C60 (100 muM), human chondrocytes were incubated with interleukin-1beta (10 ng/ml) or H(2)O(2) (100 muM), and chondrocyte activity was analyzed. An animal model of OA was produced in rabbits by resection of the medial meniscus and medial collateral ligament. Rabbits were divided into 5 subgroups: sham operation or treatment with C60 at 0.1 muM, 1 muM, 10 muM, or 40 muM. The left knee joint was injected intraarticularly with water-soluble C60 (2 ml), while, as a control, the right knee joint received 50% polyethylene glycol (2 ml), once weekly for 4 weeks or 8 weeks. Knee bone and cartilage tissue were prepared for histologic analysis. In addition, in the OA rabbit model, the effect of C60 (10 muM) on degeneration of articular cartilage was compared with that of sodium hyaluronate (HA) (5 mg/ml). RESULTS: C60 (100 muM) inhibited the catabolic stress-induced production of matrix-degrading enzymes (matrix metalloproteinases 1, 3, and 13), down-regulation of matrix production, and apoptosis and premature senescence in human chondrocytes in vitro. In rabbits with OA, treatment with water-soluble C60 significantly reduced articular cartilage degeneration, whereas control knee joints showed progression of cartilage degeneration with time. This inhibitory effect was dose dependent, and was superior to that of HA. Combined treatment with C60 and HA yielded a significant reduction in cartilage degeneration compared with either treatment alone. CONCLUSION: The results indicate that C60 fullerene is a potential therapeutic agent for the protection of articular cartilage against progression of OA. PMID: 17907184 [PubMed - as supplied by publisher] Who loves ya. Tom Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore! http://tinyurl.com/a3cc3 DEAD PEOPLE WALKING http://tinyurl.com/zk9fk "A few years ago I discussed in length with a man about the **need** for an efficient safe iron chelator and whether his work with the buckyball could be somehow developed into an iron chelator .. Voila .. ?" As usual you do not understand at a fundimental level the abstracts you post. This means often that the conclusion you draw is completely off the mark and even refutes the iron causes all disease because people eat meat notion. For you it is at best a throw of the dice as to what it will be. Therefore, I would not book passage to oslo just yet based on the abstract. On Oct 7, 1:57 pm, ferr...@paris.com wrote: As usual you do not understand at a fundimental level the abstracts you post. << Now I say it is a chelate for iron and you say it .. isn't .. Write this down .. fullerol forms a complex with iron .. but as you say .. "this isn't an iron chelate" .. now write this down again .. fullerol forms a complex with iron .. do you see any difference in what you say .. and what they say .. ? It is just the opposite .. <<snip>> fullerol formed insoluble complex with Fe3+ and Fe2+. <<snip>> FULLEROL AS A LIGAL AND RADICAL TRAPPER A. or evi 1, J. anadanovi -Brunet2, M. Vojinovi -Miloradov1, R. Kova evi 1, S. Mirkov1, N. Andri 1, G. Bogdanovi 3 1Faculty of Sciences, University of Novi Sad, Yugoslavia 2Faculty of Technology, University of Novi Sad, Yugoslavia 3Institute of Oncology Sremska Kamenica, Yugoslavia ABSTRACT Chemical modification of hydrophobic fullerene C60 molecule yielded many water-soluble adducts exhibiting the variety of biological activities. In this paper the results of chemical investigation of fullerol (polyhydroxy derivate of C60 (C60 (OH) 24) as a ligand for the iron cations as well as a radical scavenger were presented. Polyhydroxylated fullerene C60 water-soluble derivative, fullerol, C60(OH)24, as well as fullerol-Fe+2 and Fe3+ complexes were synthetized and characterized in the laboratories of the Chemistry Department, Faculty of Sciences, University of Novi Sad. ESR spin "trapping" method was used to assay radical scavenging activity of the fullerol.In water, fullerol formed insoluble complex with Fe3+ and Fe2+. These complexes were thermally unstable but were soluble in high alkaline medium. At the pH values close to physiological pH, the complexes were very stable. This paper presents the hypothetical structure of the fullerol-Fe3+ complex. We also reported the experimental investigation of antioxidative properties of fullerol C60(OH)24 in the chemical model system. Relative intensity (RI) of ESR signals of PBN-OH (*-phenyl-N-t-butylnitrone-OH) adduct, in the presence of 0.88 mmol/dm3 of fullerol, were decreased by 82.2%. The intensity of the new signal was increased by time while the intensity of the second basic signal of PBN-OH adduct was decreased. Based on these spectra it can be proposed that the new signal represents a radical of fullerol C60(OH)24-.. Mechanisms of effects of fullerol in in vitro model systems could be explained in two ways: either as a chelate, or as a hydroxy radicals scavenger. Experimental proof of this hypothesis is in progress. Keywords: C60, fullerol: Antioxidant: ESR, .OH: Chelate Who loves ya. Tom Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore! http://tinyurl.com/a3cc3 DEAD PEOPLE WALKING http://tinyurl.com/zk9fk geesh! rustyrump, you are mindlessly jumping the gun on this one, too! it says fullerol is a "modification", a "derivative" of fullerene. both are not the same at all, as your subject line blurred. if you begin with a basic chemical composition and alter it, you have a different chemical composition that does something else. it also states, "This paper presents the ***hypothetical***structure of the fullerol-Fe3+ complex." it does not say the "actual" nor "proven" structure. it also states, "Experimental proof of this hypothesis is **in** progress." not that there is anything conclusively proven about it, at all. "could be explained" does not equate to IS explained! kate On Oct 7, 1:57 pm, ferr...@paris.com wrote: As usual you do not understand at a fundimental level the abstracts you post. << Now I say it is a chelate for iron and you say it .. isn't .. Write this down .. fullerol forms a complex with iron .. but as you say .. "this isn't an iron chelate" .. now write this down again .. fullerol forms a complex with iron .. do you see any difference in what you say .. and what they say .. ? It is just the opposite .. <<snip>> fullerol formed insoluble complex with Fe3+ and Fe2+. <<snip>> FULLEROL AS A LIGAL AND RADICAL TRAPPER A. or evi 1, J. anadanovi -Brunet2, M. Vojinovi -Miloradov1, R. Kova evi 1, S. Mirkov1, N. Andri 1, G. Bogdanovi 3 1Faculty of Sciences, University of Novi Sad, Yugoslavia 2Faculty of Technology, University of Novi Sad, Yugoslavia 3Institute of Oncology Sremska Kamenica, Yugoslavia ABSTRACT Chemical modification of hydrophobic fullerene C60 molecule yielded many water-soluble adducts exhibiting the variety of biological activities. In this paper the results of chemical investigation of fullerol (polyhydroxy derivate of C60 (C60 (OH) 24) as a ligand for the iron cations as well as a radical scavenger were presented. Polyhydroxylated fullerene C60 water-soluble derivative, fullerol, C60(OH)24, as well as fullerol-Fe+2 and Fe3+ complexes were synthetized and characterized in the laboratories of the Chemistry Department, Faculty of Sciences, University of Novi Sad. ESR spin "trapping" method was used to assay radical scavenging activity of the fullerol.In water, fullerol formed insoluble complex with Fe3+ and Fe2+. These complexes were thermally unstable but were soluble in high alkaline medium. At the pH values close to physiological pH, the complexes were very stable. This paper presents the hypothetical structure of the fullerol-Fe3+ complex. We also reported the experimental investigation of antioxidative properties of fullerol C60(OH)24 in the chemical model system. Relative intensity (RI) of ESR signals of PBN-OH (*-phenyl-N-t-butylnitrone-OH) adduct, in the presence of 0.88 mmol/dm3 of fullerol, were decreased by 82.2%. The intensity of the new signal was increased by time while the intensity of the second basic signal of PBN-OH adduct was decreased. Based on these spectra it can be proposed that the new signal represents a radical of fullerol C60(OH)24-.. Mechanisms of effects of fullerol in in vitro model systems could be explained in two ways: either as a chelate, or as a hydroxy radicals scavenger. Experimental proof of this hypothesis is in progress. Keywords: C60, fullerol: Antioxidant: ESR, .OH: Chelate Who loves ya. Tom Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore! http://tinyurl.com/a3cc3 DEAD PEOPLE WALKING http://tinyurl.com/zk9fk pussy numbnutz ironman wrote: > FULLEROL AS A LIGAL AND RADICAL TRAPPER > This paper presents the hypothetical structure > Mechanisms of effects of fullerol in in vitro model systems could be > explained in two ways: either as a chelate, or as a hydroxy radicals > scavenger. Experimental proof of this hypothesis is in progress. "hypothetical" "could be explaned in two ways....." "Experimental proof...." pussy, you ain't proven anything yet. The entire paper is about a study in progress. So far, everything is "hypothetical." Is that word too big for you? ... pussy tom's problem is too much asphalt --- rectal troubles. |
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