It seems this .. effect of methotrexate is well known .. it
**decreases** red blood cell production therefore .. making it ..
chemical bloodletting / reduction of red blood cell production ..

<<snip>>
methotrexate shifts iron from being utilized in hemoglobin synthesis to
liver stores
<<snip>>

Titre du document / Document title
Effect of methotrexate and folinic acid on accumulation of iron in mice
Auteur(s) / Author(s)
IQBAL M. P. ; MEHBOOBALI N. ; SULTANA F. ; KHAN F. B. ; SURREY I. A. ;
KAKEPOTO G. N. ;
Résumé / Abstract
A mouse-model was used to investigate the effect of methotrexate (MTX)
and folinic acid on accumulation of iron in young growing mice. Four
equal groups of Balb/c young male mice were treated (subcutaneously)
with either MTX, or folinic acid, or MTX plus folinic acid, or
physiological saline on every second day. After 3 weeks of treatment,
liver, spleen, kidney, small intestine, brain, skeletal muscle and
heart were removed and analyzed for iron contents using a
spectrophotometric method. When the mean values of iron in liver of
four groups were compared using one way ANOVA followed by Tukey's HSD
test, the group receiving MTX alone was found to have significantly (p
= 0.004) more accumulation of iron in liver. The group receiving MTX
plus folinic acid had iron accumulation in the liver similar to the
placebo group. However, the mean values of iron in brain, kidney, small
intestine, skeletal muscle, heart and spleen in all the groups, were
not found to be statistically different. The data indicate that MTX
shifts iron from being utilized in hemoglobin synthesis to liver
stores. Folinic acid administration 8 h post-MTX, however, prevents
this shift of iron to liver. Decreased levels of iron in plasma in mice
treated with MTX alone suggest decreased availability of iron to other
tissues for their normal growth and development.
Revue / Journal Title
Medical hypotheses  (Med. hypotheses)  ISSN 0306-9877
Source / Source
2003, vol. 61, no4, pp. 444-445 [2 page(s) (article)]
Langue / Language
Anglais

Editeur / Publisher
Elsevier, Kidlington, ROYAUME-UNI (1975) (Revue)

Localisation / Location
INIST-CNRS, Cote INIST : 18253, 35400011268084.0060

Copyright 2006 INIST-CNRS. All rights reserved

Toute reproduction ou diffusion même partielle, par quelque procédé
ou sur tout support que ce soit, ne pourra être faite sans l'accord
préalable écrit de l'INIST-CNRS.
No part of these records may be reproduced of distributed, in any form
or by any means, without the prior written permission of INIST-CNRS.

Nº notice refdoc (ud4) : 15221864

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