"Cells of older individuals are prone to oxidations"

Flow cytometric estimation of 'labile iron pool' in human white blood
cells reveals a positive association with ageing
Authors: Paschalis-Thomas Doulias a;  Chara Vlachou a;  Christina
Boudouri b;  Panagiotis Kanavaros c;  Kostas C. Siamopoulos b;
Dimitrios Galaris a
Affiliations:    a Laboratory of Biological Chemistry, University of
Ioannina Medical School, Ioannina, Greece
b Department of Nephrology, University of Ioannina Medical School,
Ioannina, Greece
c Laboratory of Anatomy-Histology-Embryology, University of Ioannina
Medical School, Ioannina, Greece

DOI: 10.1080/10715760801911649
Free Radical Research, Volume 42, Issue 3 March 2008 , pages 253 -
259

Abstract
A small part of cellular iron, usually called 'labile iron
pool' (LIP), is not securely stored and has the potential to catalyse
the formation of highly reactive oxygen species. The present work
estimated LIP levels in human white cells by using the analytical
power of flow cytometry. The method relies essentially on already
established principles but has the added value of monitoring LIP in
different subpopulations of human blood cells concurrently in a single
sample. Examination of 41 apparently healthy individuals revealed a
positive correlation between LIP levels and the age of the donors
(r=0.656, 0.572 and 0.702 for granulocytes, lymphocytes and monocytes,
respectively, p<0.0001), indicating that cells of older individuals
are prone to oxidations in conditions of oxidative stress. It is
suggested that LIP estimation may represent a valuable tool in
examinations searching for links between iron and a variety of
oxidative stress-related pathological conditions.
Keywords: Ageing; calcein; flow cytometry; human leukocytes; labile
iron pool (LIP); oxidative stress
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