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Medical Forum / General / Alternative / April 2008Broad Impact Of Oxidative Stress
Thalassemia is an iron loading disease therefore is a human model of iron overload. Quantitative determination of ortho- and meta-tyrosine as biomarkers of protein oxidative damage in beta-thalassemia. [Journal Article] Redox Rep 2007; 12(5):219-28. Matayatsuk C, Poljak A, Bustamante S, Smythe GA, Kalpravidh RW, Sirankapracha P, Fucharoen S, Wilairat P Oxidative stress in thalassemia is caused by secondary iron overload and stems from blood transfusion and increased iron uptake. In this study, we hypothesized that levels of o- and m-tyrosine, products of hydroxyl radical attack on phenylalanine, would be elevated in beta-thalassemia (intermediate). This study represents the first report in which specific markers of protein oxidative damage have been quantified in thalassemia. We used GC/MS to assay o- and m-tyrosine at the femtomole level using only a few microliters of plasma. Levels of both markers were significantly higher in patients with beta- thalassemia than in controls and were positively correlated with serum ferritin, malondialdehyde, superoxide dismutase, glutathione peroxidase and glutathione. We conclude that o- and m-tyrosine are useful biomarkers of oxidative damage to proteins in thalassemia (intermediate) and may also be useful markers in other iron overload diseases. Positive correlations between o- and m-tyrosine levels and malondialdehyde as well as antioxidants such as superoxide dismutase, glutathione peroxidase and glutathione, are indicative of the broad impact of oxidative stress on blood plasma in thalassemia, with up-regulation of antioxidant proteins probably reflecting a homeostatic response to these increased stress levels. -------------------------------------------------------------------------------- More from this journal Redox report : communications in free radical research [Redox Rep] Who loves ya. Tom Jesus Was A Vegetarian! http://tinyurl.com/2r2nkh Man Is A Herbivore! http://tinyurl.com/a3cc3 DEAD PEOPLE WALKING http://tinyurl.com/zk9fk Iranian Journal of Pediatrics Tehran University of Medical Sciences Press ISSN: 1018-4406 Vol. 17, No. s1, 2007, pp. 73-78 Bioline Code: pe07024 Full paper language: Farsi Document available free of charge Iranian Journal of Pediatrics, Vol. 17, No. s1, 2007, pp. 73-78 en Comparison of serum leptin in major β-talasemia patients and normal subjects Choobineh, H; Dehghani, SJ; Einollahi, N; Kaviani, S; Reiskarami, SR & Vahedi, S Abstract Background: Leptin, is a adipocyte-derived hormone. Exogenous leptin allows the recovery of the reproductive function. In humans, leptin correlates positively with body mass index (BMI). The aim of the study was to investigate the association of leptin with toxic effects of iron overload. Methods: In a cross sectional study in 2006, we compared the serum leptin level of thalasemic patients with normal group. Blood samples were collected from 219 patients with Cooley's anemia, (119 males, 100 females) and 137 normal subjects (86 males, 51 females). Leptin was measured by a commercial ELISA kit. Data were analyzed by SPSS software. Findings: Mean serum leptin level was 5.33± 5.02 ng/ml in thalassaemic males. It was significantly lower than controls (9.43±7.8 ng/ml) (P<0.001). Thalassaemic females had lower leptin levels (12.12±11.4 ng/ ml) than normal females subjects (14.6±13.1 ng/ml) (P<0.001). Furthermore, the physiologically positive BMI/leptin relationship disappeared in thalassaemic patients. Conclusions: It seems that the adipocytes of thalassaemic patients are unable to maintain adequate leptin production. These results suggest that adipose tissue dysfunction can be considered as one of the endocrine¬pathies affecting thalassaemic patients. Keywords Beta, -Thalassemia major, Leptin, BMI, Serum iron Who loves ya. Tom Jesus Was A Vegetarian! http://tinyurl.com/2r2nkh Man Is A Herbivore! http://tinyurl.com/a3cc3 DEAD PEOPLE WALKING http://tinyurl.com/zk9fk > Thalassemia is an iron loading disease therefore is a human model of > iron overload. [quoted text clipped - 40 lines] > > DEAD PEOPLE WALKINGhttp://tinyurl.com/zk9fk |
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