 |
 | Home | Contact Us | FAQ | Search & Site Map | Link to Us |
 | Sign In | Join | Other 45 Sites in Network |
Medical Forum / General / Alternative / May 2007Stem cells and marrow
Would / does .. ? .. phlebotomy / venesection / bloodletting ..ALSO .. 'create' .. in INCREASED numbers .. stem cells ALONG with new red blood cells .. ? So .. would the fact an 'anemic' person would have bone marrow proliferation at a higher 'renewal' rate of someone who is .. polycythemic .. ? .. because a person in iron REreplete state would be creating red blood cells FROM .. strictly .. iron / haematinic .. http://tinyurl.com/24nquv Whereas .. one creating red blood cells FROM the .. marrow .. marked by INCREASED erythropoietin PRODUCTION due to lack of iron / haematinic, would be perpetuating the self renewal of ALL cells related TO.. this 'marrow' .. process .. ? Therefore INCLUDING .. stem cells .. And on the flip side .. since increased iron seems to self renew red blood cells / erythrocytosis would this lead to a .. decreased .. amount of stem cells .. As evidenced by the low .. erythropoietin .. STILL creating red blood cells .. evidencing PLENTY of .. haematinic / iron .. 'on board' .. because a low erythropoietin is a **sign** OF .. plenty of haematinic / iron / blood **forming** .. elements. Primitive Adult Stem Cells Found In Peripheral Blood Main Category: Stem Cell Research News Article Date: 18 Apr 2006 - 0:00 PDT Biology / Biochemistry Moraga Biotechnology Corporation, an adult stem cell company based in Los Angeles, California, announces the discovery of its proprietary Blastomere-Like Stem Cells (BLSCs) circulating in the peripheral blood of mammals. The Company's scientists found that these adult stem cells were able to differentiate into most tissues and organs of the body. The Company's scientists have developed a proprietary and cost- effective method for isolating and purifying large numbers of its primitive embryonic- like stem cells from the blood without ex vivo expansion. Moraga recently announced that its BLSCs were found in large numbers throughout the body. The Company believes this new discovery is a major breakthrough for finding large numbers of primitive adult stem cells circulating in the blood. Moraga's chief executive, Dr. John F. Wong, noted that the ability to isolate these primitive embryonic-like stem cells in peripheral blood allows the Company to enter the stem cell banking business in the near future. With this new method for isolating stem cells, the Company will be able to bank an individual's stem cells by simply withdrawing a sample of their blood. The Company intends in the very near future to establish processing centers to which a donor's blood sample is shipped; the blood is processed; and, the stem cells are isolated. The BLSCs are then stored at the center for future therapeutic and diagnostic use. Dr. Wong further stated that "by circumventing the expensive process of isolating and expanding the stem cells outside the body, the Company can now travel down a shorter path towards developing a near-term solution for autologous stem cell-based therapies, from which its BLSCs may be used for treating various forms of diseases such as heart attacks, Parkinson's disease and stroke." Moraga Biotechnology Corporation http://www.moragabiotech.com -------------------------------------------------------------------- Definitions of haematinic on the Web: hematinic: a medicine that increases the hemoglobin content of the blood; used to treat iron-deficiency anemia wordnet.princeton.edu/perl/webwn -------------------------------------------------------------------- Who loves ya. Tom Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore! http://tinyurl.com/a3cc3 DEAD PEOPLE WALKING http://tinyurl.com/zk9fk Iron deprivation blocks multilineage haematopoietic differentiation by inhibiting induction of p21(WAF1/CIP1) Authors: Alcantara, Orlando; Boldt, David H. Source: British Journal of Haematology, Volume 137, Number 3, May 2007, pp. 252-261(10) Abstract: Summary Iron is required for the differentiation of HL-60 cells along the monocyte lineage in vitro, reflecting a requirement for iron in the transcriptional induction of the p21(WAF1/CIP1) gene. To determine if the same requirement holds true for differentiation in other cell lineages and for primary human CD34+ bone marrow precursor cells, we induced granulocyte differentiation by treating HL-60 cells with dimethyl sulphoxide, and erythroid or megakaryocytic differentiation by treating K562 cells with butyrate or phorbol myristate acetate, respectively. Nitro blue tetrazolium reduction, expression of haem, or expression of CD41 was used to assess granulocytic, erythroid, or megakaryocytic differentiation respectively. Purified CD34+ cells were cultured with granulocyte/macrophage-colony stimulating factor and stem cell factor to induce myelomonocytic differentiation. Iron deprivation was induced by desferrioxamine. p21(WAF1/CIP1) antisense oligonucleotides were used to inhibit p21 expression. Iron deprivation blocked p21 induction as judged by real-time polymerase chain reaction assays. In addition, both iron deprivation and p21 antisense blocked CD34+ cell differentiation. These observations were not explained by induction of widespread apoptosis under conditions of iron deprivation. We concluded that both iron and functional p21(WAF1/CIP1) are required for in vitro differentiation of human haematopoietic precursors along multiple cell lineages. Keywords: iron; haematopoiesis; p21(WAF1/CIP1); CD34+ cells Document Type: Research article DOI: 10.1111/j.1365-2141.2007.06549.x Who loves ya. Tom Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore! http://tinyurl.com/a3cc3 DEAD PEOPLE WALKING http://tinyurl.com/zk9fk > We concluded that both iron and functional p21(WAF1/CIP1) > are required for in vitro differentiation of human haematopoietic > precursors along multiple cell lineages. So iron is NECESSARY to produce the different cells in the blood???  SignatureJohn Gentile MS, M(ASCP) Laboratory Information Mgr. VA Medical Center Providence, RI yjgent@cox.net >> So iron is NECESSARY to produce the different cells in the blood??? << You have extramedullary blood production / outside of marrow and you have marrow blood production. Sooo .. when one has hemolytic anemia the marrow stores are commonly .. depleted .. and available markers used seem to point to .. iron deficiency .. gold standard / no bone MARROW iron. Sooo .. does the fact the cells are being made ELSEWHERE .. negate the fact iron is REQUIRED .. ? .. to form .. "ALL human haematopoietic precursors along multiple cell lineages" .. including therefore .. stem cells. Sooo .. the fact the **marrow** is DEVOID .. of .. iron .. DUE TO .. hemolytic anemia / hemo-lysis .. leads to a decrease in .. normal .. amounts of .. "ALL human haematopoietic precursors along multiple cell lineages" .. due TO .. lack of .. ? .. "So iron is NECESSARY to produce the different cells in the blood???" <<snip>> no stainable iron in the bone marrow <<snip>> Am J Clin Pathol 1983 Nov;80(5):743-5 Serum ferritin and sequestered stores of body iron. Rao KR, Ray VH, Patel AR A 47-year-old man with sickle cell anemia, chronic cor pulmonale, and congestive heart failure died following a short illness. A diagnosis of iron deficiency was established during life by usually accepted criteria including a low serum ferritin concentration. Autopsy showed no stainable iron in the bone marrow, liver, and the heart. Marked deposits of iron were seen in the kidneys and the atrophic spleen. These findings suggest that the serum ferritin concentration may not reflect the metabolically sequestered stores of iron in the spleen and the kidneys. PMID: 6195914, UI: 84049241 Who loves ya. Tom Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore! http://tinyurl.com/a3cc3 DEAD PEOPLE WALKING http://tinyurl.com/zk9fk > >> So iron is NECESSARY to produce the different cells in the blood??? > [quoted text clipped - 53 lines] > > DEAD PEOPLE WALKINGhttp://tinyurl.com/zk9fk >> You have extramedullaryblood production / outside of marrow and you have marrow blood production. Sooo .. when one has hemolytic anemia the marrow stores are commonly .. depleted .. and available markers used seem to point to .. iron deficiency .. gold standard / no bone MARROW iron. Sooo .. does the fact the cells are being made ELSEWHERE .. negate the fact iron is REQUIRED .. ? .. to form .. "ALL human haematopoietic precursors along multiple cell lineages" .. including therefore .. stem cells. Sooo .. the fact the **marrow** is DEVOID .. of .. iron .. DUE TO .. hemolytic anemia / hemo-lysis .. leads to a decrease in .. normal .. amounts of .. "ALL human haematopoietic precursors along multiple cell lineages" .. due TO .. lack of .. ? .. "So iron is NECESSARY to produce the different cells in the blood???"<< I believe the above hypothesis .. has been given credence by this article .. ? Curing the patient .. and so does .. **stem cell** production NOW .. increase .. too .. ? <<snip>> resulting in long-lasting transfusion-free survival <<snip>> Correction of anemia in a transfusion-dependent patient with primary myelofibrosis receiving iron chelation therapy with deferasirox (Exjade®, ICL670) Authors: Di Tucci, Anna Angela1; Murru, Roberta1; Alberti, Daniele2; Rabault, Bertrand2; Deplano, Simona1; Angelucci, Emanuele1 Source: European Journal of Haematology, Volume 78, Number 6, June 2007, pp. 540-542(3) Publisher: Blackwell Publishing Abstract: Transfusional iron overload in patients with chronic anemias can result in multiple organ failure. Experience in the management of iron overload in patients with myelodysplastic syndromes is limited, as many do not receive chelation therapy due to short-life expectancy and the difficulties associated with the administration of the current reference standard chelator, deferoxamine. There have, however, been some reports of reduced transfusion requirement associated with chelation therapy in patients with myelodysplastic syndromes and myelofibrosis. Here, we discuss a patient with primary myelofibrosis and related transfusion-dependent anemia who received chelation therapy with the once-daily oral iron chelator, deferasirox. In addition to the reduced iron levels, the patient demonstrated an unexpected reduction in blood transfusion requirement, ultimately resulting in long-lasting transfusion-free survival. Keywords: myelofibrosis; iron overload; chelation Document Type: Research article DOI: 10.1111/j.1600-0609.2007.00840.x Affiliations: 1: Unità Operativa Ematologia e Centro Trapianti Midollo Osseo, Ospedale Oncologico `Armando Businco', Cagliari, Italy 2: Novartis Pharma AG, Basel, Switzerland Who loves ya. Tom Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore! http://tinyurl.com/a3cc3 DEAD PEOPLE WALKING http://tinyurl.com/zk9fk |
Reply to this Message |
 Sign In
 Join
 My Latest Posts My Monitored Threads My Blog My Photo Gallery My Profile My Homepage Start New Thread Enable EMail Alerts Rate this Thread
|
©2008 Advenet LLC Privacy Policy - Terms of Use
This website includes both content owned or controlled by Advenet as well as content owned or controlled by third parties.