EMAIL ABUSE - how to stop          ( or is to Collusion?)

From: J.Paul Robinson (jpr@flowcyt.cyto.purdue.edu)
Date: Thu Dec 12 2002 - 08:43:08 EST
•    Next message: PAUL HALLBERG: "Sorting CHO cells?"
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•    Next in thread: Adam Treister: "Re: EMAIL ABUSE - how to stop"
•    Reply: Adam Treister: "Re: EMAIL ABUSE - how to stop"
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________________________________________
Colleagues: I am sending out a copy of a message I have just sent to
RNWAY laboratories of South Korea and all 20 worldwide distributors of
RNWAY products most of whom are highly reputable companies.

I am only sending it to you because I am going to propose to create a
small
"SCIENTISTS against EMAIL ABUSE"  type of revolutionary
action.........

I won't send you more copies of what I am going to send out, but I
will post
them in a prominent place on our website so that you can follow and
participate in the action.

I am truly sick and tired of receiving literally dozens of unsolicited
crap

messages each day.

However, receiving them from supposedly reputable companies

drives me really crazy.

These, I can do something about and I am.
The rest are virtually beyoned any reasonable solution. As you can
see, I have
started with RNWAY,

a company that I have requested a dozen times to
remove me from their abusive lists. This week they sent me an
attachment to
boot....- something they do constantly. This made me really mad.

Perhaps it was the beef I ate in the UK this week,

but I got really mad when
RNWAY filled my box yet again.....and I am going to set up a place
where
people can register copies of messages they send recording their
requests
for removal.

This record will become a documentation place you can copy
your messages to for any future legal actions.

We will code the email
address of the requestor so you don't get further abuse, but we will
publish
the list of companies that continue to abuse us.

If you have suggestions, please let me know.

Sincerely

"Mad Cow" Paul Robinson
Purdue University
---------------------------------------------------------------------------

Here is the email that I sent to RNWAYs 20 worldwide distributors
---------------------------------------------------------------------------------------------

You are receiving this message if you are associated with RNWAY
products.

We have continually requested removal from RNWAYS abusive and constant
emails.

It is against Federal law in the United States to continue to send
unsolicited emails after requests to discontinue are made. RNWAY posts
a
privacy policy that they make no attempt to enforce.

Our requests have been documented by the university on numerous
occasions.

RNWAY is now liable in the United States for severe penalties under
US law.

As a distributor of this company you may also incur loss of trade, or
reputation for the actions we are
going to take.

As scientists who buy your products,

we are going to exert our own economic power.

We will be letting members of our various associated networks that
RNWAY will be listed as an EMAIL SPAM ABUSER.

We will be recommending that all of our users do not purchase any of
their products

if they continue to abuse our email accounts.

As a scientist who uses the INTERNET as a crucial component of my
communication with others,

I am sick of abuse of my email and I have
decided that I am going to do something about it, one company at a
time.

This message has been copied to the 20 companies that distribute RNWAY
products around the world.

This is the first of a series of actions. It will also be copied to
3000 scientists

who may have an interest in stopping this type of abuse.

It will also be posted ona  public site that had last year over 5
million
access.

If you as a distributor want to make a profit on selling (scientists)
products, I suggest you ensure that the comanies you deal with don't
abuse
us. If they do, we won't buy their products and you will lose money.
Do you
hear us?

Yours sincerely
J.Paul Robinson, Professor, Purdue University
------------------------------------------------

J.Paul Robinson, PhD             PH:(765)4940757
Professor of Immunopharmacology
Professor of Biomedical Engineering
Purdue University          FAX:(765)4940517
EMAIL:jpr@flowcyt.cyto.purdue.edu
WEB: http://www.cyto.purdue.edu
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RE: Report from ISAC Meeting San Diego

From: Adam Treister (adam@treestar.com)

Date: Wed May 22 2002 - 01:45:52 EST
•    Next message: Susan DeMaggio: "Re: Core Manager's Workshop"
•    Previous message: Simon Watson: "Computer Networks"
•    Maybe in reply to: J.Paul Robinson: "Report from ISAC Meeting San
Diego"
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•    Reply: Adrian Smith: "Biotinylation reagents"
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________________________________________

[mailto:jpr@flowcyt.cyto.purdue.edu]

to you

of FLOJO land!

network cables for

entire congress.

congress.

Coffee again

provided by any vendor ever!.....

Paul,

Thanks for the kind words, but I can't take all the credit for the
CyberCafe.

Apple generously provided all the Macs.  IT departments around the
world may
say that Macs are hard to network, but we put a dozen Macs on the
Internet
in under an hour.   Apple sent top of the line G4s with Cinema
displays, and
Titanium PowerBooks, as well as a bunch of iMacs.  Imagine what the
lines
would have been like if we only had three computers, as we did in
Montpelier.

The true highlight of the cafe was the free espresso. Thanks to
Phoenix Flow
Systems, Guava Technologies, PROzyme and Becton Dickinson for
contributing
to the coffee fund. By the time we got to the coffee I had blown the
marketing budget on this endeavor, and these companies stepped in to
make
sure you had Ryan Brothers coffee instead of the swill we'd have
gotten from
the hotel.  I also extend a special thanks to Kevin Becker for
bringing the
Mar Dels to the banquet.  The best band of any ISAC I've attended.

The CyberCafe crew was Jennifer Wilshire, Maciej Simm, Adam Treat and
Amy
Hsu. They thought they were getting a leisurely week on the beach, and
ended
up working 9 to 9 every day to keep the CyberCafe running.  It was an
exhausting schedule, and they were tireless in their support of the
attendees' Internet needs.  It never would have come off without them.

Sophia Ascani and Alexandra Treister tie-dyed the 350 shirts.  Each
one is a
unique work of art, and each was hand-dipped.  Our garage floor has
the
stains to prove it.  So wear them proud, and wash them in cold water.

We've agreed to do it again at ISAC XXII in Montpelier.

I'm just thankful

this congress is only held every second year.

All this marketing crap

Just gets in the way of my programming.

Au revoir,

Adam
------------------------------------------------------------------
Adam Treister
Tree Star, Inc.
ph: 800-366-6045 intl: 1-650-591-2854 fax: 1-650-508-9186
adam@treestar.com    www.flowjo.com
------------------------------------------------------------------
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•    Previous message: Simon Watson: "Computer Networks"
•    Maybe in reply to: J.Paul Robinson: "Report from ISAC Meeting San
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•    Next in thread: Adrian Smith: "Biotinylation reagents"
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RE: Descriptive statistics
From: Adam Treister (adam@treestar.com)
Date: Thu May 30 2002 - 13:11:14 EST
•    Next message: ba cotleur: "ANSWER TO EXPORT RAW DATA"
•    Previous message: deborah foss: "CFDA-SE staining"
•    In reply to: Jan Bayer: "Re: Descriptive statistics"
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________________________________________

The important extension is that the probability binning methods
include
multivariate
comparisons.

Your points are valid improvements over the well known limitations of
the KS
algorithm, but they still look at only one dimension of the data.
This was
exactly how we started when we set out to add comparison statistics to
FlowJo.  But when we realized we should look at all of the data, and
implemented the multivariate comparisons, things worked much better.
Suddenly the software started quantifying the differences in ways that
more
closely matched human assessments, and it also became able to gate on
the
events that constitute the important differences.

http://www.flowjo.com/v4/html/comparison.html

Adam
------------------------------------------------------------------
Adam Treister
Tree Star, Inc.
ph: 800-366-6045 intl: 1-650-591-2854 fax: 1-650-508-9186
adam@treestar.com    www.flowjo.com
------------------------------------------------------------------
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Re: newbie help with software compensation
From: David Novo (dave@denovosoftware.com)
Date: Fri Jun 14 2002 - 00:24:27 EST
•    Next message: Annette Byrne: "Permeabilising Cells"
•    Previous message: Michael Herron: "Re: FITC-labelling of bacteria"
•    In reply to: Marty Bigos: "Re: newbie help with software
compensation"
•    Next in thread: Mario Roederer: "Re: newbie help with software
compensation"
•    Reply: Mario Roederer: "Re: newbie help with software compensation"
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________________________________________
Hi Marty,

Just to reassure you, FCS Express does use the linear model correctly.
It may shock you
to learn that other people, aside from your friends at Treestar, can
read Dr. Bagwell's
paper and actually understand it enough to implement the algorithms
that he describes.

FCS Express allows you to enter the files with the single stained
controls and it
calculates the matrix values based upon the data in the file. In
addition, in order to
see how slight changes in the compensation can distort your data, you
can change the
compensation values with sliders to see what happens. Its a great for
teaching people
the difference between proper, over- and under- compensated data. You
can also enter
a user defined value to smooth out the digitization errors that can
occur in the low
end of lower resolution data.

If anyone finds any problems with the compensation (which hasn't
happened yet), please
let me know and of course I will fix any errors. But until that
happens, I think that
your assumption that FCS Express uses the linear model correctly is
actually a very
good one.

-Dave

At 06:23 PM 6/12/02 -0700, you wrote:

________________________________________
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•    Reply: Mario Roederer: "Re: newbie help with software compensation"
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Advertising
From: Mario Roederer (roederer@drmr.com)
Date: Fri Nov 01 2002 - 16:46:47 EST
•    Next message: William Telford: "Re: Fluorochromes choice"
•    Previous message: Derek Davies: "RE: Fluorochromes choice"
•    Next in thread: Penney Robbins: "RE: Advertising"
•    Maybe reply: Penney Robbins: "RE: Advertising"
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________________________________________
I agree that there have been far too many commercial-oriented emails
on the list.  I appreciate the efforts of most manufacturers to
withhold from advertising on this list (plus, of course, the efforts
of Steve & Paul to filter those out).

The list, which is an outstanding forum for exchange of information,
has been occasionally used to identify substantially new products
which can significantly impact on how we do experiments.  I feel that
the Molecular Probes email of 10/30 clearly does not fall into this
category; the new product advertised was no more than a slight
modification of the existing one.  Such an email should be directed
solely to the person requesting information; if that person then
collates responses and puts it back on the list then so be it.  But
for manufacturers to directly respond in this way is
counter-productive to the goals of this list.

I would like to propose a 6-month moratorium on all emails that are
no more than advertisements.  Note that I write "would like
to"--because I'm not sure that this is possible.  I don't want to put
any additional onus on Steve or Paul to filter out the borderline
emails.  While these may be easy to identify when they come from
manufacturers, it could just as well be considered blatant
advertising when they come from a user.

Therefore, perhaps we can see if the commercial participants on this
list could exercise self-restraint rather than requesting a formal
censorship of advertising emails.

Thus, if you are a manufacturer, and you are responding to somebody's
request for information, do so privately to that person ONLY.  It is
up to the person requesting information to decide whether or not the
information received in response to the query warrants a summary on
the list.

If you are not a manufacturer, and are responding to somebody's
request for specific information, please consider whether your
response (that identifies a specific product or manufacturer) is of
general enough interest to warrant the list.  If it does not, then
simply send it privately to the person who requested information, and
let them decide whether to post the summary of responses.

In general, I urge people to err on the side of caution and send
their information only to the person who requested it.  Realize that
if several people want the same information, they can always request
it from the original poster!  I have posted queries to the list;
people have sent me emails asking me to forward to them the
responses, which I did.

This process can significantly cut down on emails that might be
viewed as too commercial.

mr
________________________________________
•    Next message: William Telford: "Re: Fluorochromes choice"
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•    Next in thread: Penney Robbins: "RE: Advertising"
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advertizing - no need to comment on this please.
From: J.Paul Robinson (jpr@flowcyt.cyto.purdue.edu)
Date: Sat Nov 02 2002 - 17:43:44 EST
•    Next message: Mike Wade: "Rat p53 antibody"
•    Previous message: cy: "CD34"
•    In reply to: Enoc_Hollemweguer@BD.com: "Re: looking for CCR7"
•    Next in thread: Donnenberg, Albert: "RE: looking for CCR7"
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________________________________________

Please don't start another "he said, she said" on advertizing. I don't
want to
have to spend half my day negotiating squabbles between intelligent
people
(and scientists)

Lets keep it down to a dull roar. This is a discussion group whose
intentions
are to facilitate information exchange and knowledge .

OK that's enough. I hope there will not be a host of comments on
this.  Get
back to the science.....

thanks
Paul Robinson
Purdue University

J.Paul Robinson, PhD             PH:(765)4940757
Professor of Immunopharmacology
Professor of Biomedical Engineering
Purdue University          FAX:(765)4940517
EMAIL:jpr@flowcyt.cyto.purdue.edu
WEB: http://www.cyto.purdue.edu
________________________________________
•    Next message: Mike Wade: "Rat p53 antibody"
•    Previous message: cy: "CD34"
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RE: Advertising
From: Penney Robbins (parneogen@excite.com)
Date: Mon Nov 04 2002 - 15:44:10 EST
•    Next message: Richard Haugland: "Re: Fluorochromes choice"
•    Previous message: Beavis, Andrew: "Positions Available at Esoterix
Inc, New Jersey"
•    Maybe in reply to: Mario Roederer: "Advertising"
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[ attachment ]
________________________________________
Well, I disagree. There aren't that many commerical e-mails, and for
us, an isolated
startup company, separated from academic institutions, one of the
commerical e-mails
was helpful, we would not have know about the product otherwise (see I
am even not
going to advertise it!).

All best,

Penney Robbins, PhD.

--- On Fri 11/01, Mario Roederer  wrote:
From: Mario Roederer [mailto: roederer@drmr.com]
To: cyto-inbox
Date: Fri, 1 Nov 2002 16:46:47 -0500
Subject: Advertising

_______________________________________________
Join Excite! - http://www.excite.com
The most personalized portal on the Web!
________________________________________
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Re: advertising
From: Alice L. Givan (Alice.L.Givan@dartmouth.edu)
Date: Tue Nov 05 2002 - 17:52:37 EST
•    Next message: Bryone Kuss: "MRK16"
•    Previous message: Karim Vermaelen: "rat blood eosinophils"
•    Next in thread: Alice L. Givan: "Re: advertising"
•    Maybe reply: Alice L. Givan: "Re: advertising"
•    Reply: Robb Habbersett: "Re: advertising"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
I would not like for Dick Haugland to feel that we are criticizing his
participation in
this list.  Molecular Probes has been a HUGE help to all flow and
imaging cytometrists.
Advice from that company has been good, generous, dependable,  and
without strings.
The new MP handbook is a fantastic source of information.  I would
hope that advice
from Dick will continue to be submitted to the whole network --- and
if he gets a bit
of free advertising from it,  then he deserves it.

Alice

Alice L. Givan
Englert Cell Analysis Laboratory
of the Norris Cotton Cancer Center
Dartmouth Medical School
Lebanon, New Hampshire NH 03756
tel 603-650-7661
fax 603-650-6130
givan@dartmouth.edu
________________________________________
•    Next message: Bryone Kuss: "MRK16"
•    Previous message: Karim Vermaelen: "rat blood eosinophils"
•    Next in thread: Alice L. Givan: "Re: advertising"
•    Maybe reply: Alice L. Givan: "Re: advertising"
•    Reply: Robb Habbersett: "Re: advertising"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
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Re: advertising
From: Alice L. Givan (Alice.L.Givan@dartmouth.edu)
Date: Wed Nov 06 2002 - 14:38:54 EST
•    Next message: Amy Raber: "degranulation-another question!"
•    Previous message: Robert Keefe: "Y. pestis"
•    Maybe in reply to: Alice L. Givan: "Re: advertising"
•    Next in thread: Robb Habbersett: "Re: advertising"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
I need to add that I was NOT suggesting that this network give any
special dispensation
to Dick Haugland and Molecular Probes.  They,  like many other
companies, are of
great benefit to the flow community.  I was suggesting that we be a
bit relaxed in
our willingness to accept subtle advertising in general  --- because
we can benefit
from being able to get advice directly from people who are at the
forefront of flow
development.  Obviously,  knowing where to draw the line can be
difficult.....

Alice

Alice L. Givan
Englert Cell Analysis Laboratory
of the Norris Cotton Cancer Center
Dartmouth Medical School
Lebanon, New Hampshire NH 03756
tel 603-650-7661
fax 603-650-6130
givan@dartmouth.edu
________________________________________
•    Next message: Amy Raber: "degranulation-another question!"
•    Previous message: Robert Keefe: "Y. pestis"
•    Maybe in reply to: Alice L. Givan: "Re: advertising"
•    Next in thread: Robb Habbersett: "Re: advertising"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
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Re: advertising
From: Robb Habbersett (robb@lanl.gov)
Date: Wed Nov 06 2002 - 14:19:13 EST
•    Next message: Richard Haugland: "Re: rat blood eosinophils"
•    Previous message: Amy Raber: "degranulation-another question!"
•    In reply to: Alice L. Givan: "Re: advertising"
•    Next in thread: Marty Bigos: "Re: advertising"
•    Reply: Marty Bigos: "Re: advertising"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
Once again Alice is ... RIGHT ON!!

Robb

At 05:52 PM 11/5/2002 -0500, Alice L. Givan wrote:

Robert C. Habbersett
Technical Staff Member
Los Alamos National Laboratory
MS-M888  BN2
Los Alamos, NM 87545
phone 505.667.0296
fax 505.665.3024
"As often happens in science, the paradox was resolved as soon as the
obvious was abandoned in the face of experimental evidence."
{from a recent review in Science}
________________________________________
•    Next message: Richard Haugland: "Re: rat blood eosinophils"
•    Previous message: Amy Raber: "degranulation-another question!"
•    In reply to: Alice L. Givan: "Re: advertising"
•    Next in thread: Marty Bigos: "Re: advertising"
•    Reply: Marty Bigos: "Re: advertising"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
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Re: advertising
From: Marty Bigos (mbigos@gladstone.ucsf.edu)
Date: Thu Nov 07 2002 - 18:44:09 EST
•    Next message: Beverly E. Barton: "Dye to use"
•    Previous message: Maciej S. Simm: "cell line for oxidative burst
studies"
•    In reply to: Robb Habbersett: "Re: advertising"
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[ attachment ]
________________________________________
I second (or probably 259th by the time my slow mail server gets this
out) this sentiment. Marty

________________________________________
•    Next message: Beverly E. Barton: "Dye to use"
•    Previous message: Maciej S. Simm: "cell line for oxidative burst
studies"
•    In reply to: Robb Habbersett: "Re: advertising"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
17:42:09 EST

Re: EMAIL ABUSE - how to stop
From: Adam Treister (adam@treestar.com)
Date: Mon Dec 16 2002 - 16:00:07 EST
•    Next message: PAUL HALLBERG: "Summary: Sorting CHO cells"
•    Previous message: Mojgan Shaiegan: "RBC phenotyping by flow"
•    In reply to: J.Paul Robinson: "EMAIL ABUSE - how to stop"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
On Thursday, December 12, 2002, at 05:43 AM, J.Paul Robinson wrote:

Paul,

Sounds like you're advocating fighting disease by eradicating the
antigen instead of boosting the immune response.  You can organize all
you want on eliminating the pest, but until they put a stamp tax on
email, a better approach is to let the
messages be out there, but have them filtered to oblivion before you
ever see them.

In your case, the postmaster at Purdue is probably already filtering
millions of messages a day that come to the thousands of email users
on
campus. They are probably capable of shutting down any RNWAY mail, and
spreading the word to other postmasters that they also should filter
those messages.

So if you can get the IT people at the university to tighten their
sieve, that's best. Otherwise you have to switch to an email program
that has good junk filters.  I think I get 500+ messages a day, and
only 10 to 20 make it past the junk filter.
Until last summer I was using Outlook Express and spam was a huge
problem. Since then I switched to the free Mail program in OS X, which
just added special features for spam detection and removal.  Its
probably 97% effective, and I haven't found any false positives.
So, of course, the best answer is to get a Mac  :)

I'm sure the PC mail clients are addressing this issue as well.  I
believe there are central databases of offenders so programs can learn
from others which messages to delete.   I would imagine this is the
most important feature in any email program sold these days, so I bet
Eudora or other third party mail programs have this solved.

There's a lot of information on the subject at:

http://spam.abuse.net/

Whether you fight the problem on the server or the client, it
definitely is worth getting it cleaned up.   I found it screwed up my
whole communications process because every time I wanted check email,
I
had to wade through dozens or hundreds of useless ads.

Adam

---------------------------------------------------
Adam Treister
adam@treestar.com
www.flowjo.com  800-366-6045
---------------------------------------------------
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•    Previous message: Mojgan Shaiegan: "RBC phenotyping by flow"
•    In reply to: J.Paul Robinson: "EMAIL ABUSE - how to stop"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
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•    ead ] [ subject ] [ author ] [ attachment ]
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Re: DNA analysis softwares
From: Adam Treister (adam@treestar.com)
Date: Thu Jun 10 1999 - 22:43:01 EST
•    Next message: Stefan Andreatta: "Re: Flow Cytometric Detection and
Sorting of Bacteria"
•    Previous message: Reece, Lisa: "FW: ortho permeafix"
•    Maybe in reply to: Ng Bee Ling: "Re: DNA analysis softwares"
•    Next in thread: C. Kevin Becker: "Re: DNA analysis softwares"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
----->>> On 10-Jun-1999, Ng Bee Ling wrote:

--------------

FlowJo in an offline analysis package that will read both EXPO (PC)
and
CellQuest (Mac) files.   You can compute the percentages in each of
the
phases (as well as under-G1 and over-G2) for any gated population.

We currently have a limitation in the ability to export all of the
cell
cycle statistics for all of the samples to a spreadsheet in a single
step,
but that is implemented in the next release.

More info at:
http://www.treestar.com/flowjo/html/cellcycle.html

I get a lot of requests for the references to the models, so let me
give
them here:

1) Watson Pragmatic
Cytometry 8:1-8 (1987)
Watson, Chambers, & Smith:  A Pragmatic Approach to the Analysis of
DNA
Histograms with a Definable G1 Peak

2) Dean Jett Fox
Cytometry 1:71-80 (1980)
Fox:  A Model for the Computer Analysis of Synchronous DNA
Distributions
Obtained by Flow Cytometry

3) Two Populations
Uses Dean-Jett model (with Fox modification) for both populations.

Adam

-----------------------------
Adam Treister
adam@treestar.com
http://www.treestar.com/flowjo
800-366-6045
-----------------------------
________________________________________
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Sorting of Bacteria"
•    Previous message: Reece, Lisa: "FW: ortho permeafix"
•    Maybe in reply to: Ng Bee Ling: "Re: DNA analysis softwares"
•    Next in thread: C. Kevin Becker: "Re: DNA analysis softwares"
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[ attachment ]
________________________________________
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17:37:11 EST

Re: EPICS and Y2K  ( summer special !)
Summer of 99 special -- FlowJo for a year for $99
From: Adam Treister (adam@treestar.com)
Date: Thu Jul 29 1999 - 15:00:16 EST
•    Next message: Keith Bahjat: "Re: facs archive media"
•    Previous message: Mika Korkeam{ki TUY: "RE: FACStar plus max sheath
pressure?"
•    Maybe in reply to: Bassem Allam: "EPICS and Y2K"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
----->>> On 29-Jul-1999, Bassem Allam wrote:

--------------

I'm working on an FCS file editor, one function of which will be
an automated date changing function.  So if there are Y2K problems
you can set back the date on the cytometer, and then change all
the files in one shot after collection.

This will be a free utility, and will do other keyword editing too,
but a big motivation is just to provide a quick and dirty solution
to the Y2K issues that will affect many of the cytometers out there.

The design spec is to just add a user-specified number of years
to the $DATE keyword, and to write it back to the file (using
4 digits for the year).  The original date field will be put in a
different keyword, and the program will also be able to restore the
file to its original state. If anyone has more elaborate requirements
than that, I'd appreciate it if you'd drop me a note.

Adam

-----------------------------
Adam Treister
adam@treestar.com
Summer of 99 special -- FlowJo for a year for $99
http://www.treestar.com/flowjo/summer99.html
650-508-9349
-----------------------------
________________________________________
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[ attachment ]
________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
17:37:18 EST
Adam triester and Dave Novo advertising a $99 promo
Clarifacation of what DAVE NOVO stated on Flowjo platform
Re: Cost-benefit ratio of FlowJo [Clarification]
From: Adam Treister (adam@treestar.com)
Date: Wed Aug 04 1999 - 16:28:49 EST
•    Next message: Robert C. Leif, Ph.D.: "Forwar Scatter is often not
even Forward Scatter was RE: Very basic question"
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________________________________________
If I may clarify...

Judging from the phone calls I'm getting, Cariappa may
have implied that FlowJo is a cross-platform package.
I think he meant that FlowJo reads data collected on any
cytometer, whether it was hooked to HP, PC or Mac.  FlowJo
itself still runs only on the Mac.

This may be unclear, because we are running a Summer
$99 promotion, in conjunction with DeNovo Software

.  They sell the PC based analysis package: FCS Express. So, the
solution I can offer you for Windows is at:
  <http://www.denovosoftware.com/>

The promotion is a good deal on either platform.

We are working on Win-FlowJo, but it's not close to ready.
The bad news: there is no announced release planned for
a Windows product in the foreseeable future.
The good news:  more for the Mac is coming pretty soon.

Adam

----->>> On  4-Aug-1999, Cariappa Annaiah wrote:

--------------

-----------------------------
Adam Treister
adam@treestar.com
http://www.treestar.com/flowjo
800-366-6045
-----------------------------
________________________________________
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even Forward Scatter was RE: Very basic question"
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Report from ISAC Meeting San Diego
From: J.Paul Robinson (jpr@flowcyt.cyto.purdue.edu)
Date: Wed May 08 2002 - 15:24:55 EST
•    Next message: Michael Kuhn: "Re: fluorochromes in food"
•    Previous message: stemsort: "CGES 2nd Meeting"
•    Next in thread: Adam Treister: "RE: Report from ISAC Meeting San
Diego"
•    Maybe reply: Adam Treister: "RE: Report from ISAC Meeting San
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•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
________________________________________
•
•    Colleagues:
•
•    Hello from sunny California and the ISAC XXI congress. This message
•    comes to you from the CYBER CAFE generously provided by Adam
Triester
•    of Tree Star, Inc of FLOJO land! Adam has made a bank of 12
computers, a
•    wireless network and, lots of network cables for laptops. He has a
T1 fast
•    line and is providing FREE access for the entire congress. The room
is
•    ALWAYS full and is definitley the most popular place in the
congress. It has
•    nothing to do with the outstanding FREE coffee from Ryan Bros,
Coffee
•    again generously provided by Adam. This is the best facility
provided by any
•    vendor ever!.....so long live FLOJO.....and more free coffee and
internet
•    access....
•
•
•    This message  contains several important pieces of information
including
•    information about  the proposed Cytomics initiative that Professor
Paul Smith
•    has established.  For link, see the end of this message.
•
•
•    This is the last year that I have served as a councilor so I  want
to thank you
•    all for the opportunity of serving on this important committee. I
highly
•    recommend those who are interested in participating in the society
•    management to seek election to these posts. I want to congratulate
the new
•    council members and express my thanks to those leaving their posts.
Lisa
•    Staiano-Coico has done a great job as President and we thank her for
her
•    dedication. Maria Pallavicini has been elected as the new president
and we
•    congratulate her. She will be running the next congress in
Montpellier
•    France. Congratulations to Frank Traganos was elected Treasure to
replace
•    Maria. Harry Crissman has done an outstanding job of organizing this
•    meeting, which by the way, has attracted a record 1100 plus
attendees.
•
•
•    New councilors are Paul Smith, Jan Gratama, John Nolan and Bob
Zucker.
•
•
•    I want to draw your attention to an important opportunity that Paul
Smith is
•    organizing. It is known as the CYTOMICS EU FRAMEWORK 6 INITIATIVE.
if
•    you are interested in participating in ANY way - from anywhere in
the world,
•    please go to the Purdue main page and click on the GREEN button.
Enter
•    your info and we will contact you. This is NOT an obligation, only
an
•    expression of interest in participating. Please do this URGENTLY as
we
•    need the info within a couple of weeks. The direct link is
•
•    <underline><color><param>0000,8000,0000</param>http://
www.cyto.purdue.edu/flowcyt/eucytomics/</underline><color><param>0000,0000,0000<
/param>
•
•
•    Best Regards
•
•    Paul Robinson
•
•    Purdue
•
•    J.Paul Robinson, PhD             PH:(765)4940757
•
•    Professor of Immunopharmacology
•
•    Professor of Biomedical Engineering
•
•    Purdue University          FAX:(765)4940517
•
•    EMAIL:jpr@flowcyt.cyto.purdue.edu
•
•    WEB: http://www.cyto.purdue.edu
•
•    <nofill>
•    J.Paul Robinson, PhD             PH:(765)4940757
•    Professor of Immunopharmacology
•    Professor of Biomedical Engineering
•    Purdue University          FAX:(765)4940517
•    EMAIL:jpr@flowcyt.cyto.purdue.edu
•    WEB: http://www.cyto.purdue.edu
•
•
•    -- End --
•    text/enriched attachment: stored

________________________________________
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•    Previous message: stemsort: "CGES 2nd Meeting"
•    Next in thread: Adam Treister: "RE: Report from ISAC Meeting San
Diego"
•    Maybe reply: Adam Treister: "RE: Report from ISAC Meeting San
Diego"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
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Announcing User Forum at Verity Software House, Inc.
From: VSH - Tech Support (tech@vsh.com)
Date: Tue Mar 12 2002 - 11:17:49 EST
•    Next message: Adrian Smith: "UV excitable dyes for surface
phenotyping - ELF-97?"
•    Previous message: bernadette bellette: "[TLR2 and 4 on murine
Langerhans Cells?]"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
Hello, Flow-ers,

Verity Software House is pleased to announce the creation of a Verity
User's
Forum on our web site at www.vsh.com/forum.  Explore features, discuss
applications, and share "tricks of the trade" with new and experienced
users
alike!  With a vast user network, answers and ideas are only a few
clicks
away.  Stop by and say hello, and we'll all benefit from our
collective
experience.  It's another example of Verity's commitment to customer
support!

Have a great day everyone!

Don

Donald J. Herbert
Technical Support Manager
Verity Software House, Inc.
PO Box 247
45A Augusta Road
Topsham, ME, USA  04086
Phone: (207) 729-6767 ext.190
Fax:   (207) 729-5443
email:  tech@vsh.com
web: www.vsh.com
________________________________________
•    Next message: Adrian Smith: "UV excitable dyes for surface
phenotyping - ELF-97?"
•    Previous message: bernadette bellette: "[TLR2 and 4 on murine
Langerhans Cells?]"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
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Re: Wich differences between Cell Quest and Flow Jo?
From: Adrian Smith (A.Smith@centenary.usyd.edu.AU)
Date: Mon Mar 04 2002 - 19:52:55 EST
•    Next message: Van Bockstaele, Dirk: "RE: Bascteria sorting ?"
•    Previous message: Nigel Blackhall: "Re: plant protoplast sorting"
•    In reply to: Simona Ronzoni: "Wich differences between Cell Quest
and Flow Jo?"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________

FlowJo uses a hierarchical rather than a logical gate structure, ie
you don't work by creating two gates and then combining them, rather
you place the first gate, open the gated plot and place the second
gate directly on that plot.

The best way to get into FlowJo is to do the tutorial that can be
downloaded from the web-site (<http://www.flowjo.com/tutorial.html>).
Until you have got your head around the fundamental differences
between the programs it is very hard to compare them.

Adrian
________________________________________
•    Next message: Van Bockstaele, Dirk: "RE: Bascteria sorting ?"
•    Previous message: Nigel Blackhall: "Re: plant protoplast sorting"
•    In reply to: Simona Ronzoni: "Wich differences between Cell Quest
and Flow Jo?"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
17:41:27 EST
FlowJo Analysis Seminar
From: Martha Kirby (mkirby@nhgri.nih.gov)
Date: Mon Apr 08 2002 - 15:51:42 EST
•    Next message: Voorn, J.: "conclusions to the ELISPOT interest
inventory"
•    Previous message: Kurtz, James: "[7AAD viability for CD34]"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
To Flow Cytometry Group,

Hi! Just wanted to let you know that Jennifer Wilshire from Tree
Star, Inc. will be giving a FlowJo Software Demonstration this
Thursday, April 11 at 1:00 PM in  the 4th Floor Conference Room (room
4A46), Bldg. 49, NIH, NHGRI.  This Flow Cytometry Analysis Seminar is
open to all interested.

Thanks,

Martha

Disclaimer of Endorsement: Reference herein to any specific
commercial products, process, or service by trade name, trademark,
manufacturer, or otherwise, does not necessarily constitute or imply
its endorsement, recommendation, or favoring by the United States
Government. The views and opinions of authors expressed herein do not
necessarily state or reflect those of the United States Government,
and shall not be used for advertising or product endorsement purposes.

--
Martha Kirby
NIH, NHGRI, GMBB
Bldg. 10, Rm. 2C19
9000 Rockville Pike
Bethesda, MD 20892

301 402-2264
mkirby@NHGRI.NIH.GOV
________________________________________
•    Next message: Voorn, J.: "conclusions to the ELISPOT interest
inventory"
•    Previous message: Kurtz, James: "[7AAD viability for CD34]"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
17:41:36 EST