ISAC Congress Marketing Crap Gets In the way of Programming

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Mitch Haynes - 01 Dec 2008 03:36 GMT

Purdue University Cytometry Laboratories virtually no trash emails on
this group.

If anyone sends trash mail, they are rapidl;y
deleted from the list and their access is barred.

Re: List reply-to setting.
From: J. Paul Robinson (jpr@flowcyt.cyto.purdue.edu)
Date: Tue Feb 10 1998 - 12:01:03 EST
•    Next message: Leary, James: "RE: Labeling cells with beads"
•    Previous message: Steve G. Hilliard: "Re: List reply-to setting."
•    In reply to: Mark A. Corio: "List reply-to setting."
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________________________________________
"Mark A. Corio" suggests the need for a listserve approach:

Here is my reply:

The lsit is run from my lab

and

Steve kelley send me your message.

The way our list is run is by intention.

It is designed for maximum value and least annoyance.

We believe that a message that is to be
sent to the list myst be intentioned to the list.

Otherwise individuals communicate directly.

We will not be changing this method
as it is the one favored overwhelmingly by the group.

There are virtually no trash emails on this group.

There is a very good reason for that.

If anyone sends trash mail, they are rapidl;y
deleted from the list

and

their access is barred.

This makes a very
strong incentive to keep a high quality of discussion.

My experience
with listserves is the rubbish is overwhelming!

We have considered
many times the advantages of a listserve ,

and the disadvantages far
outweigh them.

Since we are one of the older scientific discussion
groups out there, our track-record speaks for itself.

We do encourage
people to tabulate results of their questions.

They invariably do
this and we thank you.

While, I appreciate this might generate some email,

let's not fill
the box.

We are NOT going to change it anyway!!!

regards
Paul Robinson

> From:

"Mark A. Corio" <rmi@frontiernet.net>

> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> Subject: List reply-to setting.
> Date: Mon, 9 Feb 1998 17:18:29 -0500

> Steve,
> Belonging to several lists, I found something unusual when I joined this
> list....the reply-to setting is to the original author instead of the list.
> Several others mentioned it recently and I wonder if the list would like
> to have the reply-to set to the list so that more responses appear on-line
> instead of personal conversations. The list traffic goes up a little, but
> the responses are often more valuable with information than the original
> posts. Anyone else feel this would be a good idea? Is there a reason it is
> the way it is, Steve, that we shouldn't change it?
>
> Mark A. Corio
> Rochester MicroSystems, Inc.
> 200 Buell Road, Suite 9
> Rochester, NY 14624
> Tel: (716) 328-5850
> Fax: (716) 328-1144
> e-mail: rmi@frontiernet.net
> http://www.frontiernet.net/~rmi/
> ****** Designing Electronics for Research and Industry ******

J.Paul Robinson, Purdue University Cytometry Labs
Professor of Immunopharmacology
robinson@flowcyt.cyto.purdue.edu PH:765-494 6449 FAX:765-494 0517
web http://www.cyto.purdue.edu
________________________________________
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Just one more stupidity by a manufacturer
From: Mario Roederer (ROEDERER@Darwin.Stanford.EDU)
Date: Tue Jun 28 1994 - 16:59:16 EST
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________________________________________
I just wanted to relate a little story... draw from it your own
conclusions.

Here at Stanford we were enthusiastic about one of BD's new software
products, Attractors. It would be obviously useful to our ongoing
clinical trial... About 6 months ago, I went to BD to test the
software
with some of our data (collected here, using our own software), and
it worked quite well.

About 2 weeks ago, a BD salesperson stopped by with a demo version,
trying to sell us on the software. We decided to go ahead and test
it
on our data. Much to my surprise, it could not read our data files!
(It gave a generic file reading error, stating that the file was
damaged
or not an FCS file.) I called up BD and spoke with one of the
programmers.
I was informed that the decision had been made by "BD Sales" to modify
the
FCS header reading portion of Attractors so as to only read FCS files
created by BD's software (i.e., HP-generated)! (The reasoning being,
apparently,that they didn't want people with Coulters to be able to
use the
program.)

So much for our demo. And so much for the possibility that we will
be
purchasing Attractors, since we don't generate data with BD
software.
(Hey sales people at BD: do you know how many macintoshes there are
here at Stanford that could be using your software to analyze data?
50?
100?) And it also means that the market for Attractors is
significantly
limited. Is this really good "Sales" policy?

In the open-computing environment that is the general trend of this
decade, this kind of decision is rather insulting to the user
community.

Mario Roederer

(PS--the sale pricing on Attractors was also interesting: as quoted
to
us, it is cheaper to buy a total of 6 copies than to buy 3. Go
figure.)
________________________________________
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Re: exporting to excel (FlowJo)
From: Adrian Smith (A.Smith@centenary.usyd.edu.AU)
Date: Mon Jul 27 1998 - 21:01:51 EST

•Next message: Houston, Jim : "RE: Sample Differential Failure"
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unlysed sample?"
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--------------------------------------------------------------------------------

>Someone was asking about exporting from CellQuest to Excel-
>
>just to add, FYI, that its very easy to export from FLowJo into Excel; in
>fact you can easily export all
>the stats from a large number of samples very quickly.
>
>Rachel M. Gerstein

With the added advantage that all the samples in your group are listed
down
the page with the stats across - ready for plotting or further
manipulation. No more re-entering the data from each separate sample
into a
spreadsheet. This feature alone has saved me hours since I switched
to
FlowJo.

Adrian Smith - another happy FlowJo user :)

******************************************************
Adrian Smith (PhD Student)
T CELL BIOLOGY GROUP
Centenary Institute of Cancer Medicine & Cell Biology
Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA.

Ph: 61-2-9565-6197 Fax: 61-2-9565-6103
A.Smith@centenary.usyd.edu.au
******************************************************

--------------------------------------------------------------------------------

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Re-Attractors Software Technology
From: Mike Lance (Mike_Lance@bdis.com)
Date: Wed Jun 29 1994 - 11:29:26 EST
•    Next message: Robb Habbersett: "Re: Re- attractors software
NONcompatibilities"
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calcium"
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________________________________________
Subject: Time:
4:22 PM
OFFICE MEMO Re:    BD Attractors Software Technology Date:
6/29/94

Re:    BD Attractors Software Technology

In reaction to Dr. RoedererUs message about file compatibility of
BDUs
recently introduced Attractors software technology, we would like to
make
the following comments:

BDIS invested in the development of this unique software tool to
enhance the
value of its Instrument and Reagent systems. It was decided to make
the first
release of the software compatible with list mode data files that are
generated by BD CONSORT 30 and 32, as well as by BD FACStation data
management systems. This being consistent with our general Systems
approach.
The product literature clearly documents the supported
compatibilities.

In addition, we would like to point out that to our knowledge, none of
the
analysis software of the major cytometer manufacturers claim to
support
files generated from other systems.

As to pricing, we use a common volume-based pricing policy, whereby
additional copies of the software cost the end user less than the
first. 6
copies in this policy are not less expensive than 3.

Johan G. M. Christiaanse
Group Marketing Manager
Becton Dickinson Immunocytometry Systems
________________________________________
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Data Recovery
From: Steve Kelley (kelley@flowcyt.cyto.purdue.edu)
Date: Tue Sep 14 1993 - 04:26:41 EST
•    Next message: Ron Mickaels: "10BaseT Hookups for HP 3xx"
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________________________________________
I got the following message with a question about whether it was
appropriate for the mailing list. As far as I'm concerned, anything
remotely connected to cytometry is fine, including advertisements,
as long as they don't overwhelm the list. If anyone has different
opinions, we could discuss them, but in the meantime, I'll continue
to distribute anything that comes in.

Steve

=-=-=-=-=-=-=-=-=-=-=

TO USERS OF BECTON DICKINSON CONSORT 30 & 32 WORKSTATIONS:

If you have experienced loss of data for what ever reason on
Bering or HP equipment (hard drive, diskette, Bernoulli
cartridge
or tape) in the LIF or HFS format, and want that data
recovered,
contact Richard Cox at Flow Cytometry Support for details.

************************************************************
* FLOW CYTOMETRY SUPPORT *
* PO Box 3450 *
* Saratoga, CA 95070-1450 *
* *
* Voice: 408.370.6327 *
* FAX: 408.370.6876 *
* email 2359766@mcimail.com *
* *
* Contract programming for PC and HP computer systems *
* Data recovery for Consort 30/32 Workstations *
************************************************************
________________________________________
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10BaseT Hookups for HP 3xx
From: Ron Mickaels (Ron_Mickaels@bdis.com)
Date: Wed Sep 15 1993 - 00:08:54 EST
•    Next message: STAN RESS: "NON-HUMAN PRIMATE FLOW CYTOMETRY"
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________________________________________
Subject: Time:
8:37 PM
OFFICE MEMO 10BaseT Hookups for HP 3xx Date:
9/14/93
Tom Delohery writes:

> Hi,
> Is anyone out there running FACSNet over a "10BaseT, twisted pair"
> ethernet? If so, could you let me know what hardware you're using to
> interface the LANIC card on the HP to ethernet and where to purchase?
> Any help would be greatly appreciated. thanks, tom d.

==============================================================================

> Tom Delohery | Internet: tdeloher@bigmac.mskcc.org Manager, Flow
> Cytometry Core Facility | Phone: (212) 639-8729 Memorial Sloan-Kettering
> Cancer Center | Fax: (212) 794-4019

==============================================================================

The most logical way to hook up a computer with a thin coax port (like
the HP
340) to a 10Base-T network would be to use a transceiver that has a
10Base-T
connector and a coax connector. A hub would be overkill as would a
repeater.
I have tested the CTP100-T from Cabletron and it works great. A nice
feature
of this unit is that it allows you to connect up to 10 ethernet
devices on
the coax segment.

If you have several devices in close proximity to each other, you
shouldn't
have to buy multiple transceivers. The coax segment can be up to 100m
in
length. When you hook everything together, don't forget to terminate
the end
of the coax segment!

Here's what Cabletron's catalog says about the unit:

"Cabletron's CTP100-T Coax-to-Twisted Pair Transceiver is intended for
use in
10BASE-T compatible Ethernet systems. The CTP100-T supports up to 100
meters
each of coax and twisted pair cabling. As many as 10 nodes may be
connected
to the coax segment. The CTP100-T is extremely useful in environments
where
thin Ethernet cards have been purchased and installed. The
tranceiver's other
features include automatic polarity correction and indication which
detects
whether receive pair polarity has been inverted. If an error is
detected, the
CTP100-T will correct the polarity for the proper reception. In
addition, the
CTP100-T filters noise induced on the receiver pair to prevent noise-
induced
false collisions."

Here are the specs on the unit:
Type of Interface: 10Base-T(RJ45)
10Base-2(BNC)
Number of ports: 1
Weight: 7 oz.
Dimensions: 3.3"H x 5.3"W x .9"D
Max Coax Length: 100m
Max Twisted Pair: 100m
Max # of Nodes on Coax: 10
MTBF: 931,098 Hours
List Price: $600

Special Purchase Price: $375

I have lots of experience with Cabletron and they make top-notch
equipment.
You should never experience trouble with this unit!

I have talked to our rep at Cabletron and he has agreed to sell the
units at
a special rate of $375.

Contact: Jan Boldman
(408) 441-9900 ext. 1226

Tell her that you were referred by Ron Mickaels (pronounced the same
as
Michaels) from Becton Dickinson.

*******

If you donUt have an application to do FTP transfers to the Mac, you
may
want to consider TCP/Connect II from InterCon. This is the program
that I use
to receive files from FACSNet HP and IUve had good success with it.
ItUs got
lots of features that you probably donUt need and itUs kind of
expensive, but
there arenUt too many FTP/TCP/IP programs out there! The program
should be
available at Egghead software and other distributors.

*******

One other note: When specifying IP addresses for your computers,
remember to
1. generate a unique address for each node and
2. NOT use 255 or 128 in the address. For example: the address 1.4.2.4
is a
valid address, but the address 1.255.128.45 could cause problems! This
is not
to say that you can't use these addresses, but most of the liturature
that I
have read says that you can have problems if you use them. Something
to do
with routing signals to subnets.

*******

Please keep in mind that this is not an "official" Becton Dickinson
recomendation. If you try this setup and it does not work for you,
PLEASE do
not call our service department! They will not know what you are
talking
about! The first time that I hooked this stuff up to our network it
took me 2
hours to get it to work. My point is that the problem wasn't with the
equipment, it was problems with the network. If all is ok with your
network,
then it should work fine!

HAPPY CRUNCHING!

***********************************************
Ron Mickaels
Senior Research Software Engineer
Becton Dickinson Immunocytometry Systems
San Jose, California, USA
***********************************************
________________________________________
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3rd party software Companies numbers and address.
TrueFacts Software-ada Med/Newport Instruments and Client is Pheonix
Flow Product QC-Tracker
Robert C. Leif and Suzanne B. Leif
Ada- Med/Newport Instruments (USA ) it is important to understand the
Corporate relationships as noted in the posting my Client pheonix
flow has a product QC- Tracker generic FCS Reader. In 2002 there are
more executives from ISAC Congress and Purdue involved in this
product.
Purdue Cytometry Mailing List: Re: Re[2]: Leave FCS3.0 alone.My
client
Phoenix Flow has a product, QC-Tracker, which can be transformed into
a generic FCS reader, data storage system, and user programmable
data ...
www.cyto.purdue.edu/hmarchiv/1997/1762.htm - 11k - Cached - Similar
pages - Note this
3rd party sw
From: vanburen%flovax.dnet@rocdec.roc.wayne.edu
Date: Thu Mar 11 1993 - 10:14:42 EST
•    Next message: auger@ux1.cso.uiuc.edu: "Position Opening"
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________________________________________
In response to Ivan Shaw's 'new' question:

Some 3rd party flow cytometry software vendors are:

Verity Software House, Inc.
10 New Lewiston Road
Topsham, Maine 04086
207 729 6767 voice
207 729 5443 fax

Phoenix Flow Systems
11575 Sorrento Valley Road, Suite 208
San Diego, CA 92121
619 453 5095 voice
619 259 5268 fax

Cytomation, Inc.
400 E. Horsetooth Rd., Suite 100
Fort Collins, CO 80525
800 822 9902 voice
303 226 2322 fax

TrueFacts Software, Inc.
1011 Boren Ave., Suite 193
Seattle, WA 98104
800 252 5248 voice
206 621 9665 fax

All offer PC analysis software. Check recent issues of Cytometry for
product descriptions.

Eric Van Buren        vanburen%flovax.dnet@rocdec.roc.wayne.edu
Wayne State University    313 577 1009 voice
Immunology & M.Biology    313 577 1155 fax
________________________________________
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Exhibit

Since Inception of the Purdue Cytometry mailing list Vertiy Software
House has been allowed to Sell Software, hardware, Market courses and
hire sales Reps through the list.. Verity Software House, Inc. is the
exclusive distributor for TechTeam's Trupolid software. The 3rd
party software market has been controled through the mailing list and
ISAC Congress. Setting FCS Data Standards has caused many disputes due
to personal and financial intrest. Posting will provide evidence of
the 3rd party software developers worked together to build and control
the market through the list.

From: Benjamin C. Hunsberger (75450.167@CompuServe.COM)
Date: Thu Sep 30 1993 - 14:42:55 EST
•
•
•    Next message: david w. galbraith: "TIFF files"
•    Previous message: cjgros@ccmail.monsanto.com: "ISAC E-mail query"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
Verity Software still has a few openings for the fall WinList
Course.
Anyone interested in attending should call or FAX as soon as possible
(It
is expected to fill up quickly).

Information and a registration form can be FAXed to you upon
request.
Tuition cost is $400.00 per person. The class will have a maximum of
15
students with a lot of hands-on work.

FAX 207-729-5443
Phone 207-729-6767
________________________________________
•    Next message: david w. galbraith: "TIFF files"
•    Previous message: cjgros@ccmail.monsanto.com: "ISAC E-mail query"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]

Exhibit 8 Notice of new New Software Release and Notices of Early
releases are provided through the list

Software -New releases
From: Benjamin C. Hunsberger (75450.167@CompuServe.COM)
Date: Fri Oct 29 1993 - 10:44:42 EST
•    Next message: Brian Hall: "For Sale: 1989 Coulter Profile II"
•    Previous message: jm5@maties.sun.ac.za: "SOFTWARE PROGRAMS FOR FLOW
CYTOMETRY"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
Verity Software House, Inc. has just released WinList 2.0 and ModFit
5.2.
All registered users will be receiving update notices by mail. We
have new
demo disks for these products. Any one wishing information and/or a
free
demo may leave a message on Internet or FAX us at 207-729-5443.
Please
include your FAX number.

We have also produced two video tutorials (VHS format) for WinList.
Tape 1
is at the beginner - intermediate level, Tape 2 is at the intermediate
-
advanced level. Each tape is 2 hr's long and has a price of $75.00.
New
orders for WinList received by Jan. 1, 1993 will receive both tapes
free.

Verity Software House, Inc. is now the exclusive distributor for
TechTeam's
Truploid software. Truploid allows you to quantitatively evaluate
your DNA
listmode files for drift, "bulges" and standard deviation then if
desired
remove part of the acquisition and create a new file. Again you may
leave
a message on Internet or FAX us for more details.

Don Herbert
Verity Software House, Inc.
10 New Lewiston Road
Topsham, ME 04086
Phone 207-729-6767
FAX 207-729-5443
________________________________________
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[ attachment ]

Re: FACSCAN -> PC
From: Mike Lance (Mike_Lance@bdis.com)
Date: Wed Jun 16 1993 - 21:33:23 EST
•    Next message: Christopher A. Worth: "follow up HP>> PC"
•    Previous message: Leon Martin: "Re: FACSCAN -> PC"
•    Maybe in reply to: Nick Viner: "FACSCAN -> PC"
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________________________________________
Subject: Time:
6:11 PM
OFFICE MEMO RE>FACSCAN -> PC Date:
6/16/93

In reply to Nick Viner's recent message, I have one specific
clarification.

"Verity have a package called WHPPC which provides both the board and
software for $895, although we could get the board alone for less and
use
software provided by BD, I understand."

BD does not have any software for the IEEE-488 solutions. The
original
"HPPCLink" software written here was never brought to market and now
resides
in the archives at CMU as a non-supported shareware program. That
software
was designed for Pascal version 3.1 based systems. We provided the
source
code to Verity Software House to update the program, and they have
revised
the Pascal portion of the code to work with Pascal 3.2 systems, and
wrote a
program on the PC side that runs under Microsoft Windows. The
transfer
software requires both the HP and PC programs, along with an IEEE-488
board
and cable. The above quote from Nick is fine but the software need
come
from either the CMU library or Verity.

As for Verity's WHPPC product, I have recieved no negative feedback
from
customers who are using it and they seem very happy with it as a low
cost
solution for connecting a single PC to an HP.

Mike Lance
Becton Dickinson
________________________________________
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Since 1993 list members participated in a effort to develop third pary
software. It is important to rember which members participate as they
grow a colation throughout the years.
Bob Murphy is currently the President of ISAC Congress 2008. Our
Email was sent to Bob Murphy whom was the President Elect at the time
and J. Paul Robinson was the President of ISAC Congress. The email
was intercepted by Steve Kelly and sent to J. Paul Robinson.
J. Paul Robinson J. Paul Robinson ... ViewFriday, September 28,
2007 9:37:32 AM To:mitchell haynes Cc:david_at_kanecki2.com;
skelley_at_flowcyt.cyto.purdue.edu
Steve
what is this email - it came to me with Bob Murphy's name associated
with it. It seems to be an advertisement, this junk mail, and it
seems to have been modified by you ...
So I guess I am confused. was this sent to the list, or do you have
an details about it
i am concerned about these junk messages going out
to our members, - if they are using our lists, I will deal with them
appropriately, but I am not happy any info you can give thanks
paul
FCS File headers
From: kelley@mrc.com
Date: Tue Jun 15 1993 - 12:49:21 EST
•    Next message: Christopher A. Worth: "hp>>PC"
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fcs file headers"
•    Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
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________________________________________
Alice Givan asks about Bob Murphy's program which reads key words
from an FCS file. The program is called FCSTAB and allows you to
read a number of keywords including such things as beginning and
ending time of acquisition, filename, instrment settings, etc. and
save them to a file or print them out in table format. The copy I
have is version 3.0 and is running on a MicroVax II operating VMS
5.3. I do not know if there are other versions of this program
which will run on other platforms. I am able to read files from
the Consort30 once they are transferred to the MicroVAX via HPVAXLINK.
I am willing to copy this program to a TK50 for anyone who can use it
providing you send me the TK50 to put it on.

**********************************************************************
                        *
Keith A. Kelley            kelley@mrc.com * A wink is as good
Miles Research Center        Ph 203-937-2872 * as a nod to a
400 Morgan Lane Fax 203-937-6923 * blind horse.
West Haven CT 06516                *
                        *
**********************************************************************
________________________________________
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________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
17:25:57 EST

Eric Martz at at umass maintained the list of Free FlowCytometry
software.
Nov 12, 2001 ... The Catalog of Free Flow Cytometry Software moved to
the Purdue University Cytometry Laboratories.

Purdue University Cytometry Laboratories has an International
influence on the 3rd party software market. The most Powerful Avenues
of Marketing has be derived through ISAC Congress, Usergroups,
Listservers, NIH, Government sponsered programs ie Bioscope and the
most Influnecial powerful and abused Purdue University Cytometry
Mailing List.

PC->GPIB->Paintjet
From: Eric Martz (emartz@microbio.umass.edu)
Date: Fri Jul 29 1994 - 19:22:39 EST
•    Next message: Eric Martz: "Vantage Question"
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Profile sample data files"
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[ attachment ]
________________________________________
Does anyone else out there have:
1. A PC with a GPIB/IEEE-488 card in use to transfer data files to/
from
the HP computer which came with your B-D FACS?
2. An HP Paintjet used to print from the HP FACS computer?

If so, did you know that you can print directly from the PC to the
Paintjet with your existing hardware and software (no more $)?
I recently realized that I could, and (after several tech support
rounds
with National Instruments gpib_support@natinst.com) figured out how
to do it. I'd be happy to share the details with anyone interested.
You can print beautiful color graphics from any Windows software, e.g.
Joe Trotter's WinMDI, as well as text (in color!) from Wordperfect,
and
from any DOS software which emits plain text or has a Paintjet driver.

Being able to use WinMDI on your B-D data and print the results in
color
on your existing Paintjet makes it worth purchasing the GPIB card,
if you can come up with $450 or so. It will also allow you to use
MFI.
(HPPCLINK software for both the HP and PC is free, courtesy B-D.)

The HP Deskjet (which would work directly from your PC parallel port)
is
really no better for most purposes, and is slower at high resolution
(7 minutes/page). The Paintjet from Windows via IEEE-488 takes 6
minutes
for a full page at 180 dpi (highest Paintjet resolution), and 3
minutes
for 90 dpi which is fine most of the time.

If you want to try out WinMDI or MFI on a PC before you set up the
HP<->PC transfer hardware, the data files in the MFI tutorial can be
used with either. Geoff Osborne's file OSBORNE2.LMD is really pretty
with 3-colors, CD3, CD4, and CD8.

You can get WinMDI by anonymous ftp from flosun.salk.edu in /pub/pc.
MFI and MFITUTOR from flowcyt.bio.umass.edu in /pub/flowcyt/mfi.

/*- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Eric Martz, Professor of Immunology emartz@microbio.umass.edu
Dept Microbiology Voice: 413-545-2325 FAX: 413-545-1578
Morrill IVN 203, Box 35720, Univ Massachusetts, Amherst MA 01003-5720
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
*/
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Looking for Web sites/other resources on Cytometry Development for
FlowJo
From: Adam Treister (treister@CAMIS.Stanford.EDU)
Date: Thu Nov 17 1994 - 11:43:27 EST
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________________________________________
I'm new to the field, and starting a data analysis package for the
FACS facility
at Stanford.

I'm looking for resources that will kick start my learning process.
I'd
appreciate any pointers to Web sites I should visit, other software I
should try
out, or other programmers who'd live to collaborate in building object
libraries, or maybe cool features you think should be in flow
cytometry
software, but that you haven't seen before.

Thanks for the help,

Adam Treister

Stanford Shared FACS Facility
________________________________________
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Posting of Advertisment, Software development and Sales has allways
been allowed on the Purdue University Cytometry Laboratories Mailing
list. Third party software and License Fees has been discussed since
its inception..

Exhibit Verity Software House Hiring Software Sales Reps

Position available-Verity Hiring
From: Benjamin C. Hunsberge (75450.167@compuserve.com)
Date: Fri Jun 09 1995 - 08:49:24 EST
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________________________________________
Due to continued growth, Verity Software House, a flow cytometry
software
manufacturer, is seeking a friendly, motivated person for a Sales/
Customer
Service position. Previous experience in cytometry would be looked
upon
favorably but is not required. We are located close to Maine's scenic
coast,
only a short distance from the mountains and only 2.5 hours from
Boston.
Interested persons should send resumes to
Verity Software House, Inc.
PO Box 247, Topsham, Maine, U.S.A. 04086
Telephone: (207) 729-6767
Fax: (207) 729-5443
________________________________________
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This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
17:30:32 EST

stock in Cytomation
From: bonnie@FOUNDATION.SDSTATE.EDU
Date: Tue Aug 01 1995 - 08:46:59 EST
•    Next message: /G=Gerhard/S=Nebe-von-Caron/OU=1890CHPE/O=TMGB.URC/
@LANGATE.gb.sprint.com: "Re: DAPI/uptake of nucleic acid stains in
viable bacteria"
•    Previous message: Thomas Delohery: "ISAC XVIII Abstracts"
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________________________________________
**************************************************************************
Note from the Coordinator:

I'm pretty uncomfortable about using the list for business and
finance, even when it's related to cytometry, but I'm much more
uncomfortable about blocking messages.

I understand why people
object to messages such as this, and only ask that the list not
be consumed for the next month with complaints and arguments about
what people should or shouldn't post. I also ask that we try
to keep our focus on generating and disseminating knowledge
about cells and cytometry.

Steve
***************************************************************************

As a courtesy to the flow community, I am posting this novel notice
of
interest on the flow newsgroups first.

I am pleased to offer 153,000 shares of Cytomation, Inc. for sale in
lots of
300 shares at $1.50 US per share plus transaction costs.

This is a unique opportunity to invest in the future of flow
cytometry.
However, I must caution that this is high risk stock and the
purchaser
must be able to absorb the loss of the total investment.

Having said that, if you are interested please contact me at:

bonnie@foundation.sdstate.edu

Bonnie Lievan
Cytomation, Inc. founder
________________________________________
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Cytomation stock
From: Donald E. Mosier (dmosier@scripps.edu)
Date: Wed Aug 02 1995 - 13:15:05 EST
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________________________________________
Whatever the appropriateness of this posting (I find it
inappropriate), it
must be stated that this solicitation violates a host of Federal Trade
Commission regulations, and that any responsible underwriter would
have
blocked this transmission.
________________________________________
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Stock in Cytomation
From: Brian Hall (bhall@csn.net)
Date: Thu Aug 03 1995 - 18:47:23 EST
•    Next message: TARARA@MMU.dnet.mayo.edu: "fluorescence intensity
question"
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________________________________________
On Tuesday, August 1, Bonnie Lievan published a message to the flow
user
list, offering Cytomation shares for sale.

Bonnie's signoff implied that
she was representing Cytomation. We wish to make it clear that Bonnie
has
not been associated with Cytomation for several years, that she does
not
represent Cytomation in any way whatsoever and that the shares she
offered
for sale are her personal property.

Unfortunately, we have no say in what
Bonnie chooses to do with her shares, nor do we endorse any value she
places on them. We had absolutely no part in the placement of the
August
1 message and regret any confusion that may have been generated by the
message. Moreover, we do not endorse the use of the internet for this
type of activity.

Brian Hall
Vice President
Cytomation, Inc.
________________________________________
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Why WinList?
From: Abby Allen (allen@cbr.med.harvard.edu)
Date: Tue Mar 18 1997 - 15:25:02 EST
•    Next message: Vadim Chromiak: "B.D.I.S. Home Page"
•    Previous message: Maryalice Stetler-Stevenson: "(no subject)"
•    Next in thread: Larry Seamer (CYTOM): "Re: Why WinList?"
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________________________________________
Hello everyone!

My boss just returned from a cytometry meeting where she was
sufficiently impressed with Verity Software's WinList software.
Currently, I am using Cellquest and Elite software. What is it about
WinList that would impress my boss over Cellquest and the Elite
software. What special features does it have and do I need it?

Once again, I appreciate your insight, information, etc.
Thankyou!
Abby
--
**********************************************************************
Abby Allen
Center for Blood Research
800 Huntington Ave.
Boston, MA 02115

allen@cbr.med.harvard.edu
**********************************************************************
________________________________________
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Re: Why WinList?
From: Larry Seamer (CYTOM) (LSEAMER@COBRA.UNM.EDU)
Date: Wed Mar 19 1997 - 03:31:22 EST
•    Next message: Laborator Citometrie: "RE: FTP "reget" option"
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________________________________________
In reply to your question:

I have no experience CellQuest. But I do have experience with ELITE
software and WinList. My experience with the ELITE software is with
the
standard DOS version. With the caveat that 3rd party ELITE
for Windows software exists. And, I understand, Coulter is readying
their
own version of the software for Windows 3.1 or '95, I will share my
views.

In general there are 3 major reasons to choose WinList over Elite
software (DOS version). First is versatility. WinList allows the user
to create any number of 1,2, or 3 parameter displays of any size
anywhere
on the screen. They can be shaded, line-drawings, big dots, little
dots, etc. WinList allows complex gating logic and complex calculated
parameters. The user can change the names of the parameters, the
channel
resolution of the histograms, can create color eventing on single
parameter histograms as well as dot-plots. The second major reason is
that Winlist runs under Windows (either 3.1 or '95). This allows the
user
to cut and paste with other Windows programs to create presentation
graphics and link calculations with spreadsheets such as Excel. And
third, WinList will analyze data from virtually any instrument. So
those
of us that have both BD and Coulter cytometers can use one analysis
package for both.

Larry Seamer
Technical Director, Flow Cytometry Core
University of New Mexico, CRTC

On Tue, 18 Mar 1997, Abby Allen wrote:

> Hello everyone!
>
> My boss just returned from a cytometry meeting where she was
> sufficiently impressed with Verity Software's WinList software.
> Currently, I am using Cellquest and Elite software. What is it about
> WinList that would impress my boss over Cellquest and the Elite
> software. What special features does it have and do I need it?
>
> Once again, I appreciate your insight, information, etc.
> Thankyou!
> Abby
> --
> **********************************************************************
> Abby Allen
> Center for Blood Research
> 800 Huntington Ave.
> Boston, MA 02115
>
> allen@cbr.med.harvard.edu
> **********************************************************************

________________________________________
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________________________________________

WinList in Sydney
From: Adrian Smith (A.Smith@centenary.usyd.edu.AU)
Date: Thu Mar 20 1997 - 00:28:11 EST
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________________________________________
Hi All,
    We are preparing some figures for publication and we would like to
adjust the compensation on some data. We have been led to believe that
WinList can do this post-aquisition. First, is this true and do we
need to
have single colour samples saved? Second, if it is possible, is there
anyone in the Syndey region with WinList who would be willing to let
us use
it for a couple of hours?
Replies can be sent directly to my supervisor (Barbara Fazekas, email
-
B.Fazekas@centenary.usyd.edu.au).
Thanks
Adrian Smith

******************************************************
Adrian Smith (PhD Student)
T CELL BIOLOGY GROUP
Centenary Institute of Cancer Medicine & Cell Biology
Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA.

Ph: 61-2-9565-6197 Fax: 61-2-9565-6105

******************************************************
________________________________________
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17:33:55 EST

RE: Why WinList?
From: Tom Mc Closkey (thomasm@nshs.edu)
Date: Thu Mar 20 1997 - 13:19:25 EST
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________________________________________
--- On Tue, 18 Mar 1997 15:25:02 -0500 Abby Allen
<allen@cbr.med.harvard.edu> wrote:

What is it about

>WinList that would impress my boss over Cellquest and the Elite
>software. What special features does it have and do I need it?

One feature of Winlist is the ability to perform n-color post
acquisition
compensation, which circumvents the limitations of instrument
subtraction
circuitry, which is often quite limited.

Tom

--------------------------------------------------------
Thomas W. Mc Closkey, Ph. D.
Director, Flow Cytometry
North Shore University Hospital
Biomedical Research Center
350 Community Drive
Manhasset, Long Island, New York 11030
ph: 516-562-4844 [office]; 516-562-1135/4641 [lab]
3/20/97 10:22:07 AM
E-mail: thomasm@nshs.edu
--------------------------------------------------------
________________________________________
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Cytometry software
From: softflow@wavefront.com
Date: Thu Apr 17 1997 - 04:11:28 EST
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________________________________________
Dear Flow Cytometry User,

The latest versions, FCAP-list v1.30 and FCAP-easy v1.00 flow
cytometry programs have been saved on our WEB site:
http://www.wavefront.com/~softflow
The installer files of both programs can be downloaded automatically
from the site in a self-extracting format, ready for installation.

In addition, the HPtoMac disk conversion software and the FCB
interpreter, two free software applications, are also available
on the site. HPtoMac allows you to copy files from HP-formatted
diskettes onto the Macintosh HD. The current version allows you to
set the four character file type to the desired character sequence.

FCB is an interpreter for the newly developed flow cytometry
programming
language, FCAP BASIC. FCB is a DOS application.

The FCAP-list User's Guide document has been saved on the site in
form of WWW pages:

http://www.wavefront.com/~softflow/FCLGuide

Yours Sincerely,

Gyorgy Lustyik
Soft Flow, Inc.
R&D
________________________________________
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17:33:56 EST

CellQuest versions
From: Adrian Smith (A.Smith@centenary.usyd.edu.AU)
Date: Mon May 05 1997 - 19:22:20 EST
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[ attachment ]
________________________________________
Hi All,
    Can anyone tell me what the latest version of CellQuest is? (I
think it is 3.1?). Also, what are the changes/advantages/
disadvanatages
between 3.0 and 3.1?
Thanks
Adrian

******************************************************
Adrian Smith (PhD Student)
T CELL BIOLOGY GROUP
Centenary Institute of Cancer Medicine & Cell Biology
Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA.

Ph: 61-2-9565-6197 Fax: 61-2-9565-6105

******************************************************
________________________________________
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________________________________________
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17:33:57 EST

Cytomation MoFlow Information
From: Jim Houston (jim.houston@stjude.org)
Date: Wed Jun 04 1997 - 11:55:52 EST
•    Next message: Irene J. Check, Ph.D.: "anti-human hemoglobin H"
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________________________________________
To All Users:

If you currently own a MoFlo instrument , could I please get a comment
about how pleased you are and if it has meet your needs as for as its
performance and has Cytomation itself met your needs as far as
Technical/Service response. Is this a good instrument for a novice
user or
is it better to have a veteran handle it? Any comments will be
appreciated.

Please send me an E-mail message to jim.houston@stjude.org. All
responses
will be kept in confidence.

Thanks ahead to all who can help.

Jim Houston
Associate Scientist
St. Jude Children's Research Hospital
Cell and Gene Therapy Lab

jim.houston@stjude.org
________________________________________
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Analysis software for the Mac Not yet released Verity Winlist for Mac
From: Dr William Smith (williams@ichr.uwa.edu.au)
Date: Mon Sep 08 1997 - 20:10:16 EST
•    Next message: Mark A. Miller: "Hoechst/apoptosis summary (long)"
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________________________________________
I asked a question regarding the above some time ago and received some
helpful replies, thanks.
It seems that the programs divide into two price categories, the
expensive
(CellQuest, MacLAS, FCAP List, and Verity's WinList for the Mac, not
yet
released) and the relatively inexpensive, namely FlowJo and FCAP Easy.
We have looked at both of the latter since our facility is primarily
PC
based and we just wanted some additional packages for us Mac users, so
can't afford to spend a lot.
We have been very impressed by FlowJo, from the point of view af
flexible
batch analysis, ease of use, well set up program and excellent support
via
the web site or email. We shall shortly be purchasing several copies
(when
we can arrange the funds, Adam!).
For others with similar requirements I would recommend also having a
look
at FCAP Easy, it has a few other functions at a similar price but I
have to
say I didn't find it easy to use and it didn't really suit our
purposes,
but others may prefer it.
The other solution of running WinMDI under Virtual PC or SoftWindows
we
also tried, and as others had warned us it was quite slow (on a
7200/120
PowerPC) and painfully slow printing. Also, while I still love the
graphics
of this program, the inability to save the workspace (FlowJo term) to
come
back to later is a major disadvantage now.

______________________________________________
Dr William Smith

Institute for Child Health Research
(Company Limited by Guarantee ACN 009 278 755)
Subiaco, Western Australia, 6008
PO Box 855 West Perth WA 6872

Ph 61 8 9340 8792/8388, Fax 61 8 9388 3414
email williams@ichr.uwa.edu.au
______________________________________________
________________________________________
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Re: Analysis software for the Mac
From: Dr William Smith (williams@ichr.uwa.edu.au)
Date: Wed Sep 10 1997 - 19:44:46 EST
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________________________________________

> Where can you purchase FlowJO? Or contact the manufacturer.

I'm sorry, I really should have included this information-

FlowJo Website-
http://www.treestar.com/flowjo/
Tree Star, Inc.
20 Winding Way
San Carlos CA 94070
Phone: 415-508-9349
Fax: 415-508-9186
EMail: manager@treestar.com

FCAP website-
http://www.wavefront.com/~softflow/
phone:
1-800-956-0100 (USA)
+36-72-24-00-64 (Hungary)
fax:
1-612-895-0900 (USA)
+36-72-24-00-64 (Hungary)
e-mail:
softfow@wavefront.com

Cheers, William

______________________________________________
Dr William Smith

Institute for Child Health Research
(Company Limited by Guarantee ACN 009 278 755)
Subiaco, Western Australia, 6008
PO Box 855 West Perth WA 6872

Ph 61 8 9340 8792/8388, Fax 61 8 9388 3414
email williams@ichr.uwa.edu.au
______________________________________________
________________________________________
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[ attachment ]
________________________________________
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17:34:03 EST
Publication quality graphs
From: Mario Roederer (Roederer@Darwin.Stanford.EDU)
Date: Wed Sep 24 1997 - 16:29:44 EST
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wrongs"
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[ attachment ]
________________________________________

> The Mac QuickdrawPICT 72 dpi limitation on resolution is
> the real problem using the Mac + CellQuest -> desktop
> publishing applications.

Reply to this Message

Mitch Haynes - 01 Dec 2008 06:01 GMT

Why WinList?
From: Abby Allen (allen@cbr.med.harvard.edu)
Date: Tue Mar 18 1997 - 15:25:02 EST
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________________________________________
Hello everyone!

My boss just returned from a cytometry meeting where she was
sufficiently impressed with Verity Software's WinList software.
Currently, I am using Cellquest and Elite software. What is it about
WinList that would impress my boss over Cellquest and the Elite
software. What special features does it have and do I need it?

Once again, I appreciate your insight, information, etc.
Thankyou!
Abby
--
**********************************************************************
Abby Allen
Center for Blood Research
800 Huntington Ave.
Boston, MA 02115

allen@cbr.med.harvard.edu
**********************************************************************
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Re: Why WinList?
From: Larry Seamer (CYTOM) (LSEAMER@COBRA.UNM.EDU)
Date: Wed Mar 19 1997 - 03:31:22 EST
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________________________________________
In reply to your question:

I have no experience CellQuest. But I do have experience with ELITE
software and WinList. My experience with the ELITE software is with
the
standard DOS version. With the caveat that 3rd party ELITE
for Windows software exists. And, I understand, Coulter is readying
their
own version of the software for Windows 3.1 or '95, I will share my
views.

In general there are 3 major reasons to choose WinList over Elite
software (DOS version). First is versatility. WinList allows the user
to create any number of 1,2, or 3 parameter displays of any size
anywhere
on the screen. They can be shaded, line-drawings, big dots, little
dots, etc. WinList allows complex gating logic and complex calculated
parameters. The user can change the names of the parameters, the
channel
resolution of the histograms, can create color eventing on single
parameter histograms as well as dot-plots. The second major reason is
that Winlist runs under Windows (either 3.1 or '95). This allows the
user
to cut and paste with other Windows programs to create presentation
graphics and link calculations with spreadsheets such as Excel. And
third, WinList will analyze data from virtually any instrument. So
those
of us that have both BD and Coulter cytometers can use one analysis
package for both.

Larry Seamer
Technical Director, Flow Cytometry Core
University of New Mexico, CRTC

On Tue, 18 Mar 1997, Abby Allen wrote:

> Hello everyone!
>
[quoted text clipped - 16 lines]
> allen@cbr.med.harvard.edu
> **********************************************************************

>WinList that would impress my boss over Cellquest and the Elite
>software. What special features does it have and do I need it?

One feature of Winlist is the ability to perform n-color post
acquisition
compensation, which circumvents the limitations of instrument
subtraction
circuitry, which is often quite limited.

Tom

--------------------------------------------------------
Thomas W. Mc Closkey, Ph. D.
Director, Flow Cytometry
North Shore University Hospital
Biomedical Research Center
350 Community Drive
Manhasset, Long Island, New York 11030
ph: 516-562-4844 [office]; 516-562-1135/4641 [lab]
3/20/97 10:22:07 AM
E-mail: thomasm@nshs.edu
--------------------------------------------------------
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Cytometry software
From: softflow@wavefront.com
Date: Thu Apr 17 1997 - 04:11:28 EST
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________________________________________
Dear Flow Cytometry User,

The latest versions, FCAP-list v1.30 and FCAP-easy v1.00 flow
cytometry programs have been saved on our WEB site:
http://www.wavefront.com/~softflow
The installer files of both programs can be downloaded automatically
from the site in a self-extracting format, ready for installation.

In addition, the HPtoMac disk conversion software and the FCB
interpreter, two free software applications, are also available
on the site. HPtoMac allows you to copy files from HP-formatted
diskettes onto the Macintosh HD. The current version allows you to
set the four character file type to the desired character sequence.

FCB is an interpreter for the newly developed flow cytometry
programming
language, FCAP BASIC. FCB is a DOS application.

The FCAP-list User's Guide document has been saved on the site in
form of WWW pages:

http://www.wavefront.com/~softflow/FCLGuide

Yours Sincerely,

Gyorgy Lustyik
Soft Flow, Inc.
R&D
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CellQuest versions
From: Adrian Smith (A.Smith@centenary.usyd.edu.AU)
Date: Mon May 05 1997 - 19:22:20 EST
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________________________________________
Hi All,
    Can anyone tell me what the latest version of CellQuest is? (I
think it is 3.1?). Also, what are the changes/advantages/
disadvanatages
between 3.0 and 3.1?
Thanks
Adrian

******************************************************
Adrian Smith (PhD Student)
T CELL BIOLOGY GROUP
Centenary Institute of Cancer Medicine & Cell Biology
Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA.

Ph: 61-2-9565-6197 Fax: 61-2-9565-6105

******************************************************
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Cytomation MoFlow Information
From: Jim Houston (jim.houston@stjude.org)
Date: Wed Jun 04 1997 - 11:55:52 EST
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________________________________________
To All Users:

If you currently own a MoFlo instrument , could I please get a comment
about how pleased you are and if it has meet your needs as for as its
performance and has Cytomation itself met your needs as far as
Technical/Service response. Is this a good instrument for a novice
user or
is it better to have a veteran handle it? Any comments will be
appreciated.

Please send me an E-mail message to jim.houston@stjude.org. All
responses
will be kept in confidence.

Thanks ahead to all who can help.

Jim Houston
Associate Scientist
St. Jude Children's Research Hospital
Cell and Gene Therapy Lab

jim.houston@stjude.org
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Commercial websites (was Re: BDIS Home Page)
From: James M. Simone (jmsimone@ix.netcom.com)
Date: Mon Apr 07 1997 - 20:25:54 EST
•    Next message: McGilp, Rob: "RE: Avidin conjugation of antibody"
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•    Next in thread: Gerhard Nebe-von-Caron: "Re: Commercial websites
(was Re: BDIS Home Page)"
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[ attachment ]
________________________________________
Thanks, Jeff, for letting us know about the Bio-Rad website.

One doesn't always have the time to check on all of the FCM related
sites and update our links.

You're correct to point out that commercialism should be kept at a
minimum on this list, but short notices regarding useful info, I
believe, are OK.

(Sorry if I stepped on anyone's toes re: my BDIS homepage post; just
wanted to get the info out and, no, I don't get any commission) :-)

Ciao,
Jim
________________________________________
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Re: Commercial websites (was Re: BDIS Home Page)
From: Gerhard Nebe-von-Caron (Gerhard.Nebe-von-Caron@unilever.com)
Date: Wed Apr 09 1997 - 06:52:40 EST
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________________________________________
I also don't mind that bit of commercialism as it is
quicker
dumped in the e-mail than in the waste paper basket if I
don't want it.

I still like to find a comprehensive list of commercial
suppliers. The list between Purdue and Munich is pretty
good
but not necessarily up to date. Dako has for example their
own home page and Biorad wasn't on them the last time
either. Perhaps the best way to keep up to date is to
allow
the commercials to add their address to the pages
themselves
like Paul intends to do with the other site links.

Another thing that would be nice to have is some listing of
'useful information' like the compensation tutorial of Mark
Roederer, the lin to log conversion from Dave Coder, the
useful tips for winmdi users, how to clean laser brewster
windows .... just to name a few. It also would be nice to
get some of the commercial slides on flow principles (not
pictures of gray or beige boxes on lab-benches) on the
internet with the intention of free use. Perhaps even the
flow tutorial from Coulter might be somewhere on the net.

Gerhard.Nebe-von-Caron@unilever.com

______________________________ Reply Separator
_________________________________
Subject: Commercial websites (was Re: BDIS Home Page)
Author: jmsimone@IX.NETCOM.COM at INTERNET
Date: 08/04/97 23:43

Thanks, Jeff, for letting us know about the Bio-Rad website.

One doesn't always have the time to check on all of the FCM related
sites and update our links.

You're correct to point out that commercialism should be kept at a
minimum on this list, but short notices regarding useful info, I
believe, are OK.

(Sorry if I stepped on anyone's toes re: my BDIS homepage post; just
wanted to get the info out and, no, I don't get any commission) :-)

Ciao,
Jim
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Re: Commercial websites (was Re: BDIS Home Page)
From: Alan D. Logan (a.logan@s054.aone.net.au)
Date: Thu Apr 10 1997 - 03:31:01 EST
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________________________________________
----------

>From: "Gerhard Nebe-von-Caron" <Gerhard.Nebe-von-Caron@Unilever.com>
>To: "Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>

\"jmsimone@IX.NETCOM.COM\"" <cytometry@flowcyt.cyto.purdue.edu>
(Return
requested)

>Subject: Re: Commercial websites (was Re: BDIS Home Page)
>Date: 09 Apr 1997 12:52:40 +0100
[quoted text clipped - 24 lines]
>
>______________________________ Reply Separator

_________________________________

>Subject: Commercial websites (was Re: BDIS Home Page)
>Author: jmsimone@IX.NETCOM.COM at INTERNET
[quoted text clipped - 14 lines]
>Ciao,
>Jim

The Coulter Corporation's international Web Site is to be found at,
coulter.com

Alan D. Logan
Coulter Electronics Pty. Ltd.
Sydney
Australia

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Re: BDIS Home Page
From: James M. Simone (jmsimone@ix.netcom.com)
Date: Mon Mar 31 1997 - 15:14:43 EST
•    Next message: Oleg S. Vasilyev: "Re: normal levels of HLA-DR on
CD4"
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________________________________________
If you havn't seen it yet, point your browser to the new BDIS website:

http://www.bdfacs.com/

Although the site is still under construction, one can already obtain
a
wealth of information on various topics including company info,
products, technical support, etc.

Moreover, the pages load quickly and are nicely layed out so you can
efficiently get the information you need.

Check it out!

Jim
________________________________________
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Re: BDIS Home Page
From: Joseph Chmielowski (chimo@ix.netcom.com)
Date: Mon Mar 31 1997 - 19:35:50 EST
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________________________________________
James M. Simone wrote:

> If you havn't seen it yet, point your browser to the new BDIS website:
>
[quoted text clipped - 10 lines]
>
> Jim

Also,

Check out the Coulter Web Page at

http://www.coulter.com

It is a wealth of information and even has an on-line catalog!

Joe.
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One more web page
From: NoahHadas@aol.com
Date: Mon Apr 14 1997 - 17:02:28 EST
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________________________________________
Hi All,

Sorry for being somewhat tardy with this reply, but I thought I'd let
you
know that Cytomation's web site is located at http://www.cytomation.com.
As
we are always working on it to improve its utility, any comments would
be
appreciated.

Noah
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Re: Commercial websites
From: J. Paul Robinson (PAUL@flowcyt.cyto.purdue.edu)
Date: Fri Apr 11 1997 - 12:25:16 EST
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________________________________________
There is a large listing on our web site - we try to keep it up-to-
date - anyone knowing a site we don't list let me know and we will
list them.
Regards
Paul Robinson
J.Paul Robinson, Purdue University Cytometry Labs
Professor of Immunopharmacology
robinson@flowcyt.cyto.purdue.edu PH:765-494 6449 FAX:765-494 0517
web http://www.cyto.purdue.edu
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Announcement: new web page
From: Mark A. Miller (mamiller@biochem.dental.upenn.edu)
Date: Fri Aug 29 1997 - 10:12:37 EST
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________________________________________
Our facility has finished enough of our web page to put it on line.
Please take a look at it if you get a chance. I would love to get
some
feedback.

http://biochem.dental.upenn.edu/~sdmfacs/
________________________________________
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New Macintosh-based FACS Data Analysis Program! FlowJo 97
From: Mario Roederer (Roederer@Beadle.Stanford.EDU)
Date: Tue Jun 24 1997 - 10:07:20 EST
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________________________________________
I am pleased to announce that the Macintosh-based FACS data analysis
program
that we developed here at Stanford is now available to everyone! The
program,
"FlowJo", is based on an entirely new paradigm of data analysis and
presentation, drawing in part on the long history of advanced
techniques
developed in the Herzenberg laboratory over the past 30 years. The
fundamental
basis of the program was presented at a poster session at ISAC in
Rimini last
year. I fimly believe that FlowJo will have a lasting and positive
impact on
how flow cytometry data is analyzed and presented.

The major advantage of this program is its novel approach to batch
analysis: no
more "keyboard macros"! Rather, it uses an intuitive click-and-drag
approach.
FlowJo presents an environment to you that is akin to a laboratory
notebook: it
saves all of the analyses (gatings and statistics that you do) in a
"workspace
document". When you load subsequent experiments, you can specify that
all of
the gating, graphs, and statistics that you have already performed
should be
applied automatically--saving enormous amounts of time.

FlowJo has many graph types, including "Probability Contour" plots,
using the
optimal contouring algorithm (which has been incorrectly implemented
in other
software packages). In addition, FlowJo allows you to perform
software
compensation, export gated data for analysis by other programs...
Creating
publication quality graphics is a snap: you can easily copy simple or
complex
graphical layous, or tables of statistics, into drawing programs or
spreadsheets.

For more information on how to download this program, visit the web
site
"http://www.treestar.com/flowjo/". (Stanford, which is a great place
for
developing new software but not necessarily for supporting it, has
licensed
FlowJo to TreeStar, a local company committed to continued support and
development).

A fully functional version of the program can be downloaded for free.
(And yes,
it reads data files from ALL manufacturers, as long it is in FCS
format!) At
this site listed above, you can also download or view a tutorial that
will show
you how powerful this program is.

mr
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Exhibit 7
Discussion of FCS Files and mention of BD files by Mario Roederer.
Note: He is now on the ISAC Congress Data Standards Committee and
works in the NIH he has Personal and Financial intrest.
How to make Attractors read FCS files
From: Mario Roederer (Roederer@Beadle.Stanford.EDU)
Date: Mon Jun 09 1997 - 17:36:22 EST
•    Next message: Kevin G Waddick: "Re: double label for dendritic cells
and MVE virus antigen"
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________________________________________
Peter,
this has been a previous sore spot on this mailing list. Primarily,
because BD
originally marketed a program that claimed to read FCS files, but in
fact, only
reads FCS files that originate from their data collection programs!
Perhaps
they have changed the marketing claims to be less disingenuous; I
don't know.

In comparing the files that could be read by Attractors and those that
couldn't,
it was relatively easy for me to figure out what was needed. It's a
relatively
easy fix: Simply put the string "PASCAL" at the beginning of the
value for your
$SYS keyword. For existing data files, you would need to edit them
(and this
may be less easy, since you have to make sure the length of the
keyword section
remains the same if you don't want to edit the header information).
If your
existing $SYS keyword is 6 or more characters long, then just replace
the first
6 with PASCAL.

I don't know if this is still true for the most recent versions of
Attractors,
it works for a version that we got a while ago.

Once again, shame on BD for not opening up this very fine program for
general
data analysis use.

mr
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________________________________________

Software: WinMDI 2.5 build #10
From: Joseph Trotter (trotter@scripps.edu)
Date: Fri Jun 20 1997 - 17:13:50 EST
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________________________________________
Hello All,

    FYI - there is a new version 2.5 of WinMDI (Windows Multiple Document
Interface for Flow Cytometry) available from our server. There are two
flavors:

    1) Windows 3.1
    ftp://facs.scripps.edu/pub/pc/winmdi25.exe

    2) Windows 95
    ftp://facs.scripps.edu/pub/pc/wmlatest.exe

or,

    http://facs.scripps.edu/software.html

This announcement is build #10 06/20/97 and has several patches and
bug fixes. In
addition, the program will now save windows bitmaps (less Copy/Paste
between
applications during analysis). Both flavors are the 16bit application
- the winhelp32
is needed for the Windows 95 version. I believe the bugs are gone, but
please
report anything bizarre in WinMDI's behavior to trotter@scripps.edu.

    Many thanks to everyone that helped with suggestions & bug reports!

                        Joe

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This Re: Cellquest question
From: William George Telford (apoptosi@umich.edu)
Date: Sat Jun 28 1997 - 22:10:22 EST
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•    Previous message: Ray Hicks: "Re: [Fwd: Re: Cellquest question]"
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________________________________________
Hi Mark (how is life at UM?)...

I have been able to analyze CellQuest files on Winlist 3.0, WinMDI and
PC-LYSIS, once I was able to transfer them from Mac to PC format. I
have
been able to do the transfer just by copying the files to a PC floppy
disk
or PC ZIP disk from a PowerPC, then copying them to my PC. The files
generally open fine on any program able to understand FCS 2.0 files
(although unexplained chokes do occur once in a while). We also
anticipate that transfer over the ethernet should work fine, too (once
we
get it going).

Bill

On Thu, 26 Jun 1997 KUKURUGA@medmail.med.umich.edu wrote:

> Anyone know if Winlist reads Cellquest files? How about WinMDI? Finally,
> anyu possibility that PC-Lysys reads the format (or is there some universal
[quoted text clipped - 4 lines]
> University of Michigan Flow Cytometry
> kukuruga@medmail.med.umich.edu

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Re: Cellquest question
From: Ray Hicks (rh208@cus.cam.ac.uk)
Date: Mon Jun 30 1997 - 11:49:24 EST
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Dave Coder wrote:

>Any list mode analysis program that reads FCS files (in the various
>permutations and implementations of FCS--a nice standard that provides so
>many choices) should read CellQuest files. IF AND ONLY IF the files are
>transferred as binary (NOT Mac binary) to strip the resource fork and

>transfer only the data fork. (The multiple part file type--a curiosity of
>the Mac OS--makes bona fide Mac files by definition incompatible with the
>FCS specification in that a file does not start with the characters FCS.
>The solution to the problem is simple as I outlined above.)

>Dave Coder
>dcoder@u.washington.edu

The parenthetical remarks are a bit of a slur on Macintoshes, Dave is
confusing his file types:

Bona fide mac files don't need to have a resource fork, and indeed
most
data file types don't use one ( It would appear that B-D append an
empty
[redundant] resource fork to their data files, but that doesn't
matter).
The two forks are actually separate files which are accessed using
different OS/Toolbox managers, (although the Finder handles them as
one
file,) if you open a document on a mac, you get the data fork/file -
the
resource fork/file (if it exists) doesn't get in the way.

This "multiple part file type" allows you to easily separate invariant
data
and code from application-written data all in one file, allowing
self-reading data files (which have a text reading application stuffed
into
their resource fork) and application files that can contain their own
fixed
data (eg font and menu descriptions-"resources") as well as code in
their
resource forks and variable data (such as serial numbers or
preferences)
in their data forks. Which means you can move a lot of information
around
by dragging one icon, you don't need to move a .exe., a .bin, AND
a .dat or
whatever.

MacBinary files are a different species, they are a way of
encapsulating
the data and resource forks (if extant) along with Finder information
(such
as the file's creator, type, position in window, parent directory
etc),
which governs the file's appearance. The purpose of MacBinary is to
allow
you to transfer mac files to servers not aware of the mac's file
system,
and back again to a mac, without losing anything in the process. This
requires appropriate encoding and decoding at each end. Unless they
have
translators, macintosh programs can't read or write MacBinary files,
and
don't need to. The order of data storage in MacBinary is; header,
datafork, resource fork.

MacBinary files, by definition, are incompatible with the FCS file
format,
but so are .zip files. Bona fide Mac files have no problem.

On the FCS side, are there any programs that do cover all of the
permutations of any version? Are there any that actually conform yet?
Are
there any plans to certify/verify programs' compliance?

Ray

Ray Hicks
________________________________________________________________________

|University of Cambridge |Tel 01223

330149 |

|Department of Medicine |Fax 01223

336846 |

|Level 5, Addenbrookes Hospital |e-mail

<rh208@cus.cam.ac.uk> |

_____________________________________|
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Analysis software for the Mac Not yet released Verity Winlist for Mac
From: Dr William Smith (williams@ichr.uwa.edu.au)
Date: Mon Sep 08 1997 - 20:10:16 EST
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________________________________________
I asked a question regarding the above some time ago and received some
helpful replies, thanks.
It seems that the programs divide into two price categories, the
expensive
(CellQuest, MacLAS, FCAP List, and Verity's WinList for the Mac, not
yet
released) and the relatively inexpensive, namely FlowJo and FCAP Easy.
We have looked at both of the latter since our facility is primarily
PC
based and we just wanted some additional packages for us Mac users, so
can't afford to spend a lot.
We have been very impressed by FlowJo, from the point of view af
flexible
batch analysis, ease of use, well set up program and excellent support
via
the web site or email. We shall shortly be purchasing several copies
(when
we can arrange the funds, Adam!).
For others with similar requirements I would recommend also having a
look
at FCAP Easy, it has a few other functions at a similar price but I
have to
say I didn't find it easy to use and it didn't really suit our
purposes,
but others may prefer it.
The other solution of running WinMDI under Virtual PC or SoftWindows
we
also tried, and as others had warned us it was quite slow (on a
7200/120
PowerPC) and painfully slow printing. Also, while I still love the
graphics
of this program, the inability to save the workspace (FlowJo term) to
come
back to later is a major disadvantage now.

______________________________________________
Dr William Smith

Institute for Child Health Research
(Company Limited by Guarantee ACN 009 278 755)
Subiaco, Western Australia, 6008
PO Box 855 West Perth WA 6872

Ph 61 8 9340 8792/8388, Fax 61 8 9388 3414
email williams@ichr.uwa.edu.au
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Re: Analysis software for the Mac
From: Dr William Smith (williams@ichr.uwa.edu.au)
Date: Wed Sep 10 1997 - 19:44:46 EST
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________________________________________

> Where can you purchase FlowJO? Or contact the manufacturer.

> The Mac QuickdrawPICT 72 dpi limitation on resolution is
> the real problem using the Mac + CellQuest -> desktop
> publishing applications.

This is not a general problem of the MAC, it is simply an hurdle that
most
applications don't bother to cross. Specifically, it is no problem
with FlowJo,
which can read Cellquest-generated FCS files (as well as any other
program's FCS
files). FlowJo generates graphics in PICT format, meaning that the
graphs that
you copy to your publication programs are NOT bitmaps (unless you
desire
this--it is a preference). This means that, for instance, when you
copy a
contour plot to Canvas, each contour is a separate polygon that can be
individual colorized, or have the pen pattern changed, etc. Also,
when you copy
histograms, you can easily set the pen patterns--for instance, dashed
lines,
different fill patterns etc. You can also easily resize graphs
without losing
any resolution.

For an example of the kinds of graphs that can be generated on a
Macintosh, see
"http://www.treestar.com/flowjo/documentation/graphwindow/
graphexamples.html".

mr
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MAC Data Analysis Software
From: Houston, Jim (Jim.Houston@STJUDE.ORG)
Date: Wed Oct 01 1997 - 14:34:29 EST
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________________________________________
To those who want something else besides CellQuest for prints/etc from
flow data, download the WinList demo package from Verity at VSH.com
and
give it a try. I have used WinList on the PC for years and now that I
am a MAC convert it is nice to use Winlist on the MAC.

Jim Houston

PS: there is also demo versions for the Winlist 4.0 (PC TYPE)
available
as well.
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BDIS Flow Cytometry Position
From: Burt Houtz (bhoutz@bdis.com)
Date: Thu Oct 09 1997 - 12:47:19 EST
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________________________________________
Flow Cytometry Specialist

San Jose, CA

Becton Dickinson Immunocytometry Systems, the clinical leader in flow
cytometry
and monoclonal antibodies, has an opportunity available for an
individual with
strong knowledge of flow cytometric applications including DNA
analysis and
sorting. As a member of our Customer Support-Applications Group you
will
provide telephone and field support to customers in the US for reagent
and
software products and flow cytometric applications.

The successful candidate will have a BS degree in a Biological Science
or
equivalent and at least four years of experience in flow cytometry.
In
addition, strong troubleshooting, technical writing, and communication
skills
are necessary, along with a strong working knowledge of BDIS products.
Clinical
and research experience is preferred.

Come join us in our state-of-the-art facility which includes an on-
site fitness
center. We offer a comprehensive benefits package which includes
educational
assistance, retirement, profit sharing, and 401(k). Send your resume
to Becton
Dickinson Immunocytometry Systems, Professional Staffing, Dept. CA-BH,
2350 Qume
Dr., San Jose, CA 95131-1807. FAX: (408) 435-0889, or e-mail to
bhoutz@bdis.com. We are an equal opportunity employer committed to
workforce
diversity. M/F/D/V. Visit our website at www.bdfacs.com.

________________________________________
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Potential down-time
From: Steve Kelley (SKELLEY@flowcyt.cyto.purdue.edu)
Date: Fri Oct 10 1997 - 18:19:15 EST
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________________________________________
Since I will be out of town next week, and the list is
only semi-automated, there may be interruptions and/or glitches
for a few days.

I don't have any reason to expect problems, but I always
get nervous when someone else has to try to deal with the overall
baroque nature of the email system here.

Thank you for your patience,
Steve Kelley
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DAKO Antibodies/Medimachine Summary
From: Robert Pyle (pyle@smtpgw.kfshrc.edu.sa)
Date: Sat Oct 11 1997 - 04:41:43 EST
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lymphoma"
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________________________________________
I want to thank all of those who responded to me regarding their
experiences
with DAKO antibodies and their new Medimachine. It was requested that
I
summarize the responses.

I received a total of 8 responses. 6 reported good or comparable
results to
other manufacturers with the following comments:

- Antibodies good but not optimized for flow/need tittering.
- DAKO good for providing samples of antibodies for testing
- Isotypes concentrations are not matched to the specific
antibody
concentrations so they need adjusting
- Most of the antibodies are IGG1 therefore reducing need for
many
different isotypes
- The PE appeared cleaner than other manufacturer's PE
conjugated products
- All reports were that DAKO was less expensive

2 reports of questionable results were made. One center appeared to
find that
the antibodies performed great on normals but yielded questionable
results on
clinical cases so they returned to alternative manufacturers.

As far as the Medimachine was concerned. I received two specific
responses that
were very positive. Both felt that the machine made great advances
toward a
standardized approach to cell disaggregation. One other comment also
praised it
for reducing technologist exposure to infectious samples.

Thanks again for all the comments. We have decided to give some a try
on
parallel testing.

Haywood Pyle
King Faisal Specialist Hospital and Research Center
MBC#3, Box 3354, Riyadh 11211, Saudi Arabia
pyle@kfshrc.edu.sa
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> Purdue University Cytometry Laboratories virtually no trash emails on
> this group.
[quoted text clipped - 1653 lines]
> This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
> 17:34:04 EST

Reply to this Message

Mitch Haynes - 16 Dec 2008 01:18 GMT

> Why WinList?
> From: Abby Allen (al...@cbr.med.harvard.edu)
[quoted text clipped - 262 lines]
>
> read more »

ISAC Congress Executives participage in Collusion. BD Diva Memory
Issues resolution Blocked and Filtered from Purdue University
Cytometry Labatories by ISAC Congress President J Paul Robinson and
Crew!

O.M.F.G.P.........(Obvious Misconduct For Gross Profit)

http://advenet.com/flowcytometry/blog/default.aspx

ISAC Congress Executives participage in Collusion. BD Diva Memory
Issues resolution Blocked and Filtered from Purdue University
Cytometry Labatories by ISAC Congress President J Paul Robinson and
Crew!

O.M.F.G.P.........(Obvious Misconduct For Gross Profit)

http://advenet.com/flowcytometry/blog/default.aspx

ISAC Congress Executives participage in Collusion. BD Diva Memory
Issues resolution Blocked and Filtered from Purdue University
Cytometry Labatories by ISAC Congress President J Paul Robinson and
Crew!

O.M.F.G.P.........(Obvious Misconduct For Gross Profit)

http://advenet.com/flowcytometry/blog/default.aspx

ISAC Congress Executives participage in Collusion. BD Diva Memory
Issues resolution Blocked and Filtered from Purdue University
Cytometry Labatories by ISAC Congress President J Paul Robinson and
Crew!

O.M.F.G.P.........(Obvious Misconduct For Gross Profit)

http://advenet.com/flowcytometry/blog/default.aspx

ISAC Congress Executives participage in Collusion. BD Diva Memory
Issues resolution Blocked and Filtered from Purdue University
Cytometry Labatories by ISAC Congress President J Paul Robinson and
Crew!

O.M.F.G.P.........(Obvious Misconduct For Gross Profit)

http://advenet.com/flowcytometry/blog/default.aspx

http://advenet.com/flowcytometry/blog/default.aspx

http://advenet.com/flowcytometry/blog/default.aspx

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