> laptops. He has a T1 fast line and is providing FREE access for the
> generously provided by Adam.
> so long live FLOJO.....
> it must be stated that this solicitation violates a host of Federal
> Trade Commission regulations,
[quoted text clipped - 500 lines]
>
> http://www.new.facebook.com/note.php?note_id=23879772873&ref=mf
Tim Bushnell works for Treestar for sales and development
From: Bushnell, Timothy <Timothy_Bushn...@URMC.Rochester.edu>
Date: Fri Mar 21 2008 - 07:21:57 EDT
Colleagues:
As you probably know, ISAC is holding elections this year. The
deadline
for voting is next Wednesday, March 26th. This was announced via
email
and on the ISAC homepage at:
http://www.isac-net.org/content/view/730/2/ .
I am a candidate for Biological Councilor, and would like to share
with
you my thoughts on my goals and plans should I be elected. I would
welcome anyone's comments on these goals because, as Bob pointed out,
with discussion we can refine our positions and be responsive to you,
the members of ISAC.
What I hope to bring to ISAC is an expansion of the existing programs
which serve to unite and coordinate existing affiliated societies in
North America and abroad. ISAC is uniquely positioned to support the
growth and development of regional meetings and other events to
facilitate professional networking and contacts directly related to
cytometry. Networking at such meetings is a very effective way of
communicating and sharing pertinent information about cytometry -
often
to those not affiliated with ISAC - which will increase membership as
the benefits of ISAC association are presented to an untapped market.
These meetings can also serve as a clearinghouse to discuss and
promulgate the ISAC initiatives including: education, data
presentation,
data file standardization, and biosafety. Getting this information in
the hands of the casual user will greatly enhance ISAC's efforts to
affect wholesale change in the scientific community.
I'm very enthusiastic about adding my efforts to the ISAC - most
specifically areas I have expertise in:
* Expanding the membership base in
North
America and abroad by developing ISAC initiated outreach programs, at
the local and regional level. These programs will seek to identify
the
casual user of cytometry, and provide them with the methods,
protocols
and standards that ISAC has and will continue to develop.
*
* Finding, recognizing and recruiting
cytometry facility core directors as a group vital to our membership
and
supporting them by offering ISAC models, standards, information, as
well
as an international resource to which they can come with problems,
questions and issues.
*
* Supporting and building more
regional
meetings with a greater ISAC presence via an information kiosk,
financial support for speakers, and a level of professional
camaraderie
that has been the hallmark of cytometry users around the world.
*
* Building stronger, mutually
beneficial
alliances with corporate sponsors at a regional level to facilitate a
growing base of new and existing regional groups.
My experience in developing and growing the WNYFUG from nothing, as
well
as my training as a member and program chair of the long-running
GLIIFCA
meeting provides me the perspective and skill-set necessary to
achieve
these goals.
I am an avid supporter of technology, specifically cytometry based
instrumentation, for the greater understanding of biology across the
board - however, with such change and development as we've seen in
just
20 years, that needs to be tempered with sound practices, strength of
information, networking on a global scale so that we are keenly aware
of
both developments in cytometry as well as potential pitfalls. The key
to
this level of awareness on a global scale is by seeing and serving
the
regional areas, where those 'in the trenches' can find guidance and
direction from those around the world.
I would urge all ISAC members to take a few minutes to review the
candidates and cast a vote. Your selection helps shape the future of
the Society.
Thank you in advance for your support,
Tim Bushnell
Timothy Bushnell, Ph.D.
Research Assistant Professor, Pediatrics and Oncology
Co-Director, URMC Flow Cytometry Facility
Office: 585-273-5535
Lab: 585-273-1361
Cell: 585-690-5157
Fax: 585-276-0233
http://www.urmc.rochester.edu/Aab/geneped/flow/
http://www.urmc.rochester.edu/wnyfug/
Received on Fri Mar 21 15:58:00 2008
ISAC Congress Executive Joanne Lannigan
joannelanni.._at_virginia.edu with questions
University of Virginia's Site License for FlowJo
http://www.healthsystem.virginia.edu/internet/cyto ...
FlowJo Site License
Registration Form
Complete the form below to access the University of Virginia's Site
License for FlowJo data analysis software. All fields with * must be
completed or your registration will not be processed. Once your
information has been received and verified you will receive a serial
number which you must enter in FlowJo on the computer whose hardware
address you provided. Cost per user license will be determined at the
end of the year depending on the average number of registered users
for the year. (See instructions for pricing structure) This amount
will be billed to the PTAO provided below, so please make sure the
PTAO you provide does not expire before that time. Completion of this
registration form is a valid request for services and assumes the
approval of the Principal Investigator of the PTAO account provided.
Cont
act
Joanne Lannigan joannelanni..._at_virginia.edu with questions.
*****************************************************************************************************
GLIIFCA 2007-2008 Officers and Committees
President and Scientific Program Chair Paul Wallace
Program Committee Members Mike Sramkoski, Vera Donnenberg
Executive Secretary
Co-secretary
Mary Paniagua
Ryan Duggan*
Treasurer Brian K. DuChateau
Education and CMLE Chairs Jonni Moore***********
Vendor Liasons: Karen Domenico, Tom Sawyer
Website Manager Tim Bushnell* *******************
STEERING COMMITTEE MEMBERS
Canada David Hedley , Betty-Anne McBey,Nicole McFarlane
Illinois Charles Goolsby , Maurice R. G. O'Gorman , Mary
Paniagua,
Ryan Duggan*
Indiana Lisa Green ,** Bartek Rajwa********************
Iowa Bruce Pesch , Kristi Harkins
Massachusetts Betsy Ohlsson-Wilhelm, Brian K. DuChateau
Michigan Alexander Nakeff , Louis King
Minnesota Paul Champoux
New York Paul Wallace , Timothy
Bushnell************************
Ohio Karen Domenico , Tom Sawyer , Mike Sramkoski
Pennsylvania Jonni Moore *, Vera Donnenberg
Virginia Joanne Lannigan**************************************
Wisconsin Kathy Schell , Kathy A. Muirhead , Matt Hanson
Emeritus Carleton C. Stewart, Sigrid Stewart
Review the Executive Members on ISAC Congresss
Re: clinical flow cytometry
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From: Ryan Farmer <far...@treestar.com> *********** WEBMASTER
Date: Mon Apr 14 2008 - 19:05:51 EDT
There is another site of interest along these lines.
Mycyte.org
is well established and has a variety of online texts, articles and
other links (protocols, standards, etc.). Users can expand the
variety
and
amount of information as well.
It is located at http://www.mycyte.org.
Ryan F ********WebMaster Mycyte.org
On Apr 11, 2008, at 10:47 AM, <tolda...@cox.net> <tolda...@cox.net>
wrote:
> There is a brand new on line site available for clinical flow
> resources (books,
[quoted text clipped - 66 lines]
>> replying to it and then delete the message from your computer and
>> network.
Ryan Farmer
TreeStar Inc.
340 A St. Suite 301
Ashland, OR. 97520
far...@treestar.com
(541) 201-0022 ext. 172
www.treestar.com
www.flowjo.com
www.flowdx.com
www.mycyte.org
Received on Tue Apr 15 13:58:00 2008
This message: [ Message body ]
Flow Cytometry on the Web
MyCyte.org. MyCyte.org ... Purdue. Purdue University Cytometry
Laboratories ... Added MyCyte.org. 2007-07-10 - Corrected Albert
Einstein contact : ...flowcyt.salk.edu/sitelink.html - 54k - Cached
Flow Cytometry Links - Partners AIDS Research Center
http://www.cyto.purdue.edu/hmarchiv/cytomail.htm for the
message
board ... www.mycyte.org A flow website sponsored by Treestar (makers
of Flowjo) ...www.massgeneral.org/aids/Flow Web Site links.asp -
Cached
MyCyte
MyCyte is a web portal for the flow cytometry community. ...
Purdue Cytometry List. Western New York Flow Users Group. Asia.
Australasian Flow Cytometry Group ...mycyte.org - 106k - Cached
MyCyte
MyCyte is a web portal for the flow cytometry community., Swiss
Commission declares HIV patients non-infectious, Purdue adds to the
world of flow cytometrymycyte.org/component/.../Itemid,1/limit,5/
limitstart,65 - 109k - Cached
• Purdue Cytometry Mailing List: Re: clinical flow cytometry
From : Ryan Farmer < far...@treestar.com > Date : Mon Apr 14 2008 -
19:05:51 EDT There is another site of interest along these lines.
Mycyte.org is well established and has a ...
www.cyto.purdue.edu/hmarchiv/Current/0850.htm
Flow Cytometry (FACS) Analysis Software - Life Science
Products ...
MyCyte - PolyChromatic Plot - Compensation Wizard - Daily
Dongle ... http://www.flowjo.com ... Purdue Cytometry Mailing List:
Flow analysis software. Flow ...accuratebiogene.com/search/Flow-
Cytometry-(FACS)-Analysis-Software/...
MailBucket: purdue
MailBucket
• Metroflow meeting July 1
• SAVE THE DATE• Metroflow Meeting• July 1,
2008• • The 2008 Metroflow Spring meeting will be
held on July 1, 2008 at • Princeton University, NJ.•
• This meeting will have a focus on cell life and death, with
clinical and • basic research related talks on apoptosis, the
cell cycle,• cell proliferation and autophagy.• The
technical tools, software, and reagents to study and understand
• these life and death processes will be discussed.•
• A complete speaker list and talk titles as well as
registration • information posted soon on the•
Metroflow web site:• www.metroflow.org• •
Confirmed speakers include:• • Frank Traganos,
PhD• Zbigneiw Darzynkiewicz, MD, PhD• Kathy Muirhead,
PhD• Jennifer Wilshire, PhD• • •
See
you there!• • -- • Peter Lopez• Res.
Assistant Professor of Pathology• Co-Director and Manager,
NYUCI Core Cytometry Facility• New York University School of
Medicine• 540 First Ave• Skirball 3 Administration
• New York, NY 10016• 212.263.0635 -- office•
212.263.5907 -- lab• plo...@saturn.med.nyu.edu
ISAC XXII International Congress
FlowJo software. Data showed optimal sensitivity was achieved using
PBMCs ...... CYTOMETRY: PURDUE UNIVERSITY. CYTOMETRY LABORATORIES. J.
Paul Robinson ...
doi.wiley.com/10.1002/cyto.a.20046.abs - Similar pages - Note this
Welcome to Flow Cytometry Core Facility
FlowJo Kinetics Tutorial. ? FlowJo Software Reference Manuals: ...
Purdue flow cytometry. Technical Center for those using secondary and
tertiary ...
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Flow Links - 9 visits - Mar 3
FlowJo Flojo help manual guide lesson help. ... J. Paul Robinson
Gives
WikiPedia an "A" OK, the main Flow Cytometry entry got an A, but Dr.
Robinson felt ...
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Purdue Cytometry Mailing List: SV: Thanks for the suggestions -
... cytometry/WEASELv2.html> http://www.wehi.edu.au/cytometry/WEASELv2.html
Winlist 3d from Verity House Software (www.vsh.com) Regards Tim
Timothy Bushnell, Ph.D. Director, CPBR ... Contemporary messages
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Re: Thanks for the suggestions - was rendering in 3D
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From: Adam Treister <...@treestar.com>
Date: Thu May 18 2006 - 20:20:18 EDT
On May 12, 2006, at 10:02 AM, Bushnell, Timothy wrote:
> Thanks to everyone who suggested possible software to view data in
> 3D. I’ll be trying several different platforms to see which works
[quoted text clipped - 13 lines]
>
> TimTim,
With all due respect to these solutions, you shouldn't think that
Mario could sleep at night if anyone could perform high dimensional
analysis better than FlowJo.
[Disclaimer: Yes, I live off FlowJo
sales, and that's a blatantly commercial statement, but it gets
technical from here on.]
We've played with spatial 3D plots a lot, both our own prototypes
and
others, and they just don't do a very good job of discriminating
populations. And its impossible to coherently describe the
populations you can see. I like the slice-and-dice approach that
you get by making a flipbook on X vs. Y in slices along the Z axis
(it shows up as a Quicktime movie), but that too is very difficult
to
use in a way that's better than two 2D graphs connected by a gate.
If you really want to increase your dimensionality, we're just
adding
a new "Polyvariate Plot" to the Mac version of FlowJo for next
week's
ISAC. The idea was taken from RFlowCyt. We've added interface
refinements to make it more interactive, but like any good R tool,
it'll astound and confuse you.
http://www.flowjo.com/v8/html/polyvarplot.html
The Polyvariate Plot can model transformations in any number of
dimensions. So it will produce a 3D plot, or as many dimensions as
you want (Shown below in 5D). And it projects these transformations
onto a graph window, so you can gate on them.
We're still trying to figure out the applications for this
visualization, but if you're looking for another dimension as a way
to differentiate populations, we think this is potentially much more
powerful than conventional spatial projections. This is explained
in the poster P178 at ISAC next week, or at the web page above.
Be forewarned: This is the opposite end of the sizzle/steak
spectrum. Most people use 3D graphs to make their PowerPoints look
spiffy. These graphs are absolutely impossible to explain in a
presentation.
Adam
-----------------------
A 3D plot:

A 5D plot:

Received on Fri May 19 17:58:00 2006
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From: Adam Treister a...@treestar.com
> Date: Sun Jan 04 2004 - 13:48:24 EST
> David,
[quoted text clipped - 82 lines]
> I hope that helps.
> Adam
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Purdue Cytometry Mailing List: Re: Thanks for the suggestions -
[Disclaimer: Yes, I live off FlowJo sales, and that's a blatantly
commercial statement, but it gets technical from here on.] We've
played with spatial 3D ...
www.cyto.purdue.edu/hmarchiv/2006/0939.htm
Tim Bushnell & Ryan Duggan ..... Purdue
University Cytometry Laboratories, West Lafayette, IN ...
www.gliifca.org/pdf/GLIIFCA-2004.pdf - Similar pages - Note this
MyCyte
Thanks > > Tim > > > > Timothy Bushnell, Ph.D. > > Research Assistant
Professor, ... Justin, We're working hard to finalize FlowJo
Collectors' Edition, ...
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option=com_newsfeeds&task=view&feedid=11&Itemid=70 - 133k - Cached -
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From: Adrian Smith <A.Sm...@centenary.usyd.edu.AU>
(Disclaimer: - I provided a some input into the way the platform
works and I have been beta testing FlowJo for a while now)
ISAC Congress Bilologiacl Councilor, UR, Webmaster and Great lakes
Imaging
Sales Rep?
Subject: Final Call for Flowjo order
From: "Bushnell, Timothy" <tim_bushn...@URMC.ROCHESTER.EDU>
Reply-To: Bushnell, Timothy
Date: Wed, 13 Aug 2008 16:59:51 -0400
Content-Type: multipart/alternative
Parts/Attachments: text/plain (39 lines) , text/html (40 lines)
Content-Type: text/html
This is the final call for the bulk Flowjo order. I need all orders
in by Monday afternoon (8/18). The original message is below:
------------
Hello all:
In case you missed our spring order, I am putting together another
order for Flowjo software. I will be placing it in the month of
August, to try to take advantage of the “Buy 2- get 1 free” sale.
Currently a single dongle costs $1495. However, if there are three
investigators interested in getting a dongle during this promotion,
each dongle would cost $997 (2x1495 = 2990/3 = $997). This is a
great
price. There is also no limit to the number of free dongles
available.
So, if you are interested, please email me the following information:
PI:
Contact name and number:
Account number to charge:
Regards
Tim Bushnell
--
Tim Bushnell, Ph.D.
Co-Director, URMC Flow Cytometry Facility
Office: 585-273-5535
Cell: 585-690-5157
http://www.urmc.rochester.edu/flow-core/
http://www.urmc.rochester.edu/wnyfug/
____UR_Cytometry______Subscribers Corner_____ https://lists.rochester.edu
LISTS.ROCHESTER.EDU ( UR_CYTOMETRY: 24 matches.. )
Item # Date Time Lines Subject
000139 2008-08-13 16:59 112 Final Call for Flowjo order
000138 2008-08-06 14:54 75 Summer Flowjo special
000131 2008-07-17 08:49 104 Re: Reminder: Flowjo lecture and support
000129 2008-07-17 06:24 73 Reminder: Flowjo lecture and support
000127 2008-07-14 14:05 87 Tree Star Flowjo Training/discussions,
Thursday, July 17th
000081 2008-03-14 12:21 228 Reminder: Spring Flowjo order
000079 2008-03-03 13:03 221 Spring Flowjo order
000070 2008-01-23 13:24 291 Feedback requested on Flowjo web seminars
000069 2008-01-18 09:57 249 Reminder: TODAY 1 pm - Flowjo Seminar on
DNA Analysis and Cell Proliferation
000068 2008-01-16 11:31 378 Resend: Reminder: Friday 1 pm - Flowjo
Seminar on DNA Analysis and Cell Proliferation
000067 2008-01-16 09:59 220 Reminder: Friday 1 pm - Flowjo Seminar on
DNA Analysis and Cell Proliferation
000066 2008-01-11 10:31 221 Reminder: Today 1 pm - Flowjo Seminar on
Compensation and Transformation
000065 2008-01-09 11:20 214 Reminder: Friday 1 pm - Flowjo Seminar on
Compensation and Transformation
000063 2008-01-04 10:12 193 Reminder: 1 pm - Flowjo Seminar
000061 2008-01-02 09:46 244 January is Flowjo month at URMC
000057 2007-12-04 09:13 267 January is FlowJo Month at URMC
000047 2007-10-19 10:11 209 Today is the last day to join the Flowjo
bulk order
000046 2007-10-17 10:55 284 Last call - Fall Flowjo order
000042 2007-10-09 09:29 256 2nd call - Fall Flowjo order
000040 2007-10-01 09:30 242 Fall Flowjo order
000034 2007-07-12 06:35 198 Reminder: Flowjo seminar today 1-3 pm
000032 2007-07-03 15:36 88 Post WNYFUG 2007 FlowJo Seminar
000005 2007-04-12 09:41 218 Reminder - Flowjo order
000002 2007-04-03 14:55 248 Flowjo purchase 2007
In the study of economics and market competition, collusion takes
place within an industry when rival companies cooperate for their
mutual benefit. Collusion most often takes place within the market
form of oligopoly, where the decision of a few firms to collude can
significantly impact the market as a whole. Cartels are a special
case
of explicit collusion. Collusion which is not overt, on the other
hand, is known as tacit collusion.
**********************************************************************************************
> From: J. Paul Robinson
> Reply To: j...@flowcyt.cyto.purdue.edu
[quoted text clipped - 13 lines]
> > Honestly though, well deserved praise for Mario & the Tree Star group:
> > http://www.apple.com/science/profiles/roederer
**********************************************************************************************
Re: mr on Apple web site
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From: Howard Shapiro <h...@shapirolab.com>
Date: Wed Aug 25 2004 - 18:15:38 EST
Robin Barclay wrote:
>Well .... some of us PC afficianados hate Macs just as much - personally I
>have hated them since they first made it difficult to access their DOS and
[quoted text clipped - 9 lines]
>for interfacing with lab equipment..... and they can "look cool" if you shop
>around for the right case if that's important to you.
I can't pass up the opportunity to get into the PC/Mac battle - I
hate
them
both, but there aren't really alternatives (yes, I know, there's
Linux, but
it's probably even harder to hook up a Linux system to hardware than
it is
to hook a Mac up to hardware).
Of course, what started this thread was the assertion in the piece
about
Mario on Apple's web site that there were 15,000 to 20,000 Mac-based
flow
cytometers out there. The best estimates I had from industry people
in
early 2003 when I was finishing up the 4th Edition of PFC was that
the
total number of systems from all manufacturers in use was under
20,000. If
that's correct, BD would need a 75% market share to account for the
low end
estimate of 15,000 Mac-based machines. I'd be interested to know
where
the
15,000 to 20,000 figure came from.
The old (68000 series and possibly early PowerPC) Macs were
difficult,
but
not impossible to connect to hardware; it was easier to work with the
PC's
ISA bus, which, while slow, was perfectly adequate to do most flow
cytometric data acquisition and analysis. There were decent versions
of
Forth, which was one of the first and best languages designed for
controlling hardware from mini- and microcomputers, for both PC (DOS)
and
the Mac (Forth was the first Mac programming language made
commercially
available, at a time when the only other option was buying the very
expensive Lisa from Apple on which to develop Mac software). I found
MacForth easier to program with than the Windows Forths (or other
Windows
languages, e.g., Delphi), but my old Macs used to crash all the time.
So what makes me unhappy with both Windows [and Windows machines] and
the
Mac in their current incarnations? It is now significantly harder for
mere
mortals to write software to get hardware to communicate with either
PCs or
Macs; the gain in complexity associated with the PCI bus, USB/USB2,
FireWire(IEEE 1394), etc. is greater than the gain in speed and
convenience. Also, in making the operating systems more stable (and
Win XP,
despite its security issues, is almost as stable as Mac's Unix-based
OS X),
both Microsoft and Apple elected to eliminate the ability of their
computer
hardware to respond rapidly to interrupts (latencies are now in the
tens of
thousands of instruction cycles), meaning that any really fast
hardware
attachment for either a PC or a Mac now needs to have a DSP in it,
whereas
if the fast interrupt response had been preserved, the hardware
attachments
could have been made much simpler and cheaper. Linux also takes fast
interrupt response off the table, so it doesn't represent a viable
alternative. If you go to Apple's web site and look at what data
acquisition hardware is available for Macs, particularly for Macs
running
OS X, there isn't much, and many of the companies that supported the
Mac in
that area have dropped Mac support for their newer products. That is
undoubtedly one big reason why BD's newer digital pulse processing
cytometers are running on the PC platform.
That doesn't stop anybody from analyzing FCS files on Macs. FlowJo is
well-conceived flow cytometry software; one reason it is as good as
it
is
is that it was written by people who do a lot of flow cytometry, and
cutting edge flow cytometry at that. But there are other people who
do
a
lot of good flow cytometry who have written good software, for PCs as
well
as for Macs.
For the record, I have a G4 PowerBook, which I use mostly for the
iLife
applications, which are slick. It doesn't crash more than once every
couple
of months, but the same is true of my Windows 2000 and XP systems. OS
X can
be as infuriating as Windows when one or another aspect of it goes
counter
to your intuition or to what you have gotten used to. Macs, while
somewhat
more expensive, are much better made than many PCs, and they are
certainly
aesthetically pleasing. If there were a reasonable alternative to
Microsoft's Office applications for the Mac, I might consider
switching.
The 12.1" PowerBook is a nice portable, but it's over a pound heavier
than
the Fujitsu laptop I now use, which has pretty much the same speed,
memory,
and drive capabilities (OK, not DVD-R, but I don't burn a lot of
DVDs).
However, I really wish Apple had stuck with the plan they had a few
years
back of writing a Mac operating system for Intel hardware. I think
that
died when Microsoft bailed Apple out with a few hundred mil. If it
were
possible to run OS X and XP mano a mano on the same hardware, there'd
be a
more rational basis for comparison. But, as may be the case for the
November election, minds, once made up, are not easily changed.
-Howard
Received on Thu Aug 26 16:38:00 2004
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Re: mr on Apple web site
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From: Howard Shapiro <h...@shapirolab.com
<mailto:h...@shapirolab.com?
subject=Re:%20mr%20on%20Apple%20web%20site>>
Date: Wed Aug 25 2004 - 18:15:38 EST
Robin Barclay wrote:
>Well .... some of us PC afficianados hate Macs just as much - personally I
>have hated them since they first made it difficult to access their DOS and
[quoted text clipped - 9 lines]
>for interfacing with lab equipment..... and they can "look cool" if you shop
>around for the right case if that's important to you.
I can't pass up the opportunity to get into the PC/Mac battle - I
hate
them
both, but there aren't really alternatives (yes, I know, there's
Linux, but
it's probably even harder to hook up a Linux system to hardware than
it is
to hook a Mac up to hardware).
Of course, what started this thread was the assertion in the piece
about
Mario on Apple's web site that there were 15,000 to 20,000 Mac-based
flow
cytometers out there. The best estimates I had from industry people
in
early 2003 when I was finishing up the 4th Edition of PFC was that
the
total number of systems from all manufacturers in use was under
20,000. If
that's correct, BD would need a 75% market share to account for the
low end
estimate of 15,000 Mac-based machines. I'd be interested to know
where
the
15,000 to 20,000 figure came from.
The old (68000 series and possibly early PowerPC) Macs were
difficult,
but
not impossible to connect to hardware; it was easier to work with the
PC's
ISA bus, which, while slow, was perfectly adequate to do most flow
cytometric data acquisition and analysis. There were decent versions
of
Forth, which was one of the first and best languages designed for
controlling hardware from mini- and microcomputers, for both PC (DOS)
and
the Mac (Forth was the first Mac programming language made
commercially
available, at a time when the only other option was buying the very
expensive Lisa from Apple on which to develop Mac software). I found
MacForth easier to program with than the Windows Forths (or other
Windows
languages, e.g., Delphi), but my old Macs used to crash all the time.
So what makes me unhappy with both Windows [and Windows machines] and
the
Mac in their current incarnations? It is now significantly harder for
mere
mortals to write software to get hardware to communicate with either
PCs or
Macs; the gain in complexity associated with the PCI bus, USB/USB2,
FireWire(IEEE 1394), etc. is greater than the gain in speed and
convenience. Also, in making the operating systems more stable (and
Win XP,
despite its security issues, is almost as stable as Mac's Unix-based
OS X),
both Microsoft and Apple elected to eliminate the ability of their
computer
hardware to respond rapidly to interrupts (latencies are now in the
tens of
thousands of instruction cycles), meaning that any really fast
hardware
attachment for either a PC or a Mac now needs to have a DSP in it,
whereas
if the fast interrupt response had been preserved, the hardware
attachments
could have been made much simpler and cheaper. Linux also takes fast
interrupt response off the table, so it doesn't represent a viable
alternative. If you go to Apple's web site and look at what data
acquisition hardware is available for Macs, particularly for Macs
running
OS X, there isn't much, and many of the companies that supported the
Mac in
that area have dropped Mac support for their newer products. That is
undoubtedly one big reason why BD's newer digital pulse processing
cytometers are running on the PC platform.
That doesn't stop anybody from analyzing FCS files on Macs. FlowJo is
well-conceived flow cytometry software; one reason it is as good as
it
is
is that it was written by people who do a lot of flow cytometry, and
cutting edge flow cytometry at that. But there are other people who
do
a
lot of good flow cytometry who have written good software, for PCs as
well
as for Macs.
For the record, I have a G4 PowerBook, which I use mostly for the
iLife
applications, which are slick. It doesn't crash more than once every
couple
of months, but the same is true of my Windows 2000 and XP systems. OS
X can
be as infuriating as Windows when one or another aspect of it goes
counter
to your intuition or to what you have gotten used to. Macs, while
somewhat
more expensive, are much better made than many PCs, and they are
certainly
aesthetically pleasing. If there were a reasonable alternative to
Microsoft's Office applications for the Mac, I might consider
switching.
The 12.1" PowerBook is a nice portable, but it's over a pound heavier
than
the Fujitsu laptop I now use, which has pretty much the same speed,
memory,
and drive capabilities (OK, not DVD-R, but I don't burn a lot of
DVDs).
However, I really wish Apple had stuck with the plan they had a few
years
back of writing a Mac operating system for Intel hardware. I think
that
died when Microsoft bailed Apple out with a few hundred mil. If it
were
possible to run OS X and XP mano a mano on the same hardware, there'd
be a
more rational basis for comparison. But, as may be the case for the
November election, minds, once made up, are not easily changed.
-Howard
Received on Thu Aug 26 16:38:00 2004
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RE: mr on Apple web site
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From: Fischer, Randy (NIH/NIAMS) <fisch...@mail.nih.gov>
Date: Mon Aug 23 2004 - 16:18:55 EST
Paul,
I know that for a long time, anyone who used a BD flow cytometer had
little
choice but to use a MAC-data acquisition required it. And, one of
the
best
third party software packages was FlowJo which only ran on a MAC.
Mario,
being a FlowJock, naturally was heavily exposed to this atmosphere.
However, most of our other laboratory instrumentation appears to run
on the
IBM clone type of PC-ELISA reader, luminometer, ELISpot reader, and
AFFYMETRIX machine to name the first ones that come to mind. Of
course,
because Flow is such a key component and FlowJo works so well, we
have
MACs
(and associated cinema displays) at all of our desks. So, Mario is
probably
pretty close as I guess we now run about 50% MAC, and for most things
they
do run better than our PCs for similar applications, but mostly the
two
platforms tend to be used for very disparate applications and we need
both.
Wish more science companies would move to MAC, but as Microsoft
probably
owns a minority share in most companies, that will not likely happen
in our
lifetimes.
Haven't read the article yet, but hopefully Mario remembered to cite
you for
all the work on the Flow website/mailing list.
Randy T. Fischer
NIH/NIAMS
Building 10, Room 6D50
9000 Rockville Pike
Bethesda, MD 20892
(301) 594-3537
fisch...@mail.nih.gov
> ----------
> From: J. Paul Robinson
[quoted text clipped - 40 lines]
> Have you seen our new HCS webpage?
> http://www.cyto.purdue.edu/hcs
Received on Tue Aug 24 16:18:00 2004
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*************************************************************************
J PAUL ROBINSON
DON'T WET YOUR POCKET PROTECTORS
Re: mr on Apple web site
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From: J. Paul Robinson <j...@flowcyt.cyto.purdue.edu>
Date: Mon Aug 23 2004 - 18:46:12 EST
I knew that I should not have taken Mario's bait....but its
been a long summer.....!
paul
> Paul:
> I use Macs exclusively. Between home and labs, I have 7.
[quoted text clipped - 54 lines]
> > Have you seen our new HCS webpage?
> > http://www.cyto.purdue.edu/hcs
J.Paul Robinson, PhD PH:(765)4940757
Professor of Immunopharmacology
Professor of Biomedical Engineering
Purdue University FAX:(765)4940517
EMAIL:j...@flowcyt.cyto.purdue.edu
WEB: http://www.cyto.purdue.edu
Have you seen our new HCS webpage?
http://www.cyto.purdue.edu/hcs
Received on Tue Aug 24 15:58:00 2004
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Flow Links - 9 visits - Mar 3
FlowJo Flojo help manual guide lesson help. ... in Flow Cytometry -
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Thread Sort ...
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Flow cytometer analysis on PC
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Author Topic
mlinde
Member
United States
11 Posts Posted - February 03 2006 : 6:21:13 PM
________________________________________
I'm looking into flow cytometer analysis programs for the PC and I
was
wondering whether anyone had any insight into which programs were the
most useful and worthwhile. From what I have seen, Flow-Jo and
FCSexpress are the more commonly used PC programs. Does anyone have
any experience with either of these programs? The other option would
be to purchase another Macintosh and use CellQuest. Advice, comments,
suggestions?
rakeshverma
Member
United States
93 Posts Posted - February 04 2006 : 10:46:54 PM
________________________________________
I have used CellQuest. Its a good option.
Antonio68
Member
Germany
20 Posts Posted - May 01 2006 : 11:48:43 AM
________________________________________
You can use Summit from Dako is free and gives you the possibility to
do offline compensation. However, the best flow cytometry software is
FlowJo. There is a PC (Java based) and a Mac version. The Mac version
for the moment is quite better.
lovesthelab
Senior Member
United States
804 Posts Posted - July 06 2006 : 4:59:46 PM
________________________________________
I'm not a PC person, but am an ardent flow person. My flow friends
with PCs swear by WinMidi, which is free. If you Google for free flow
cytometry software, you'll come to 2 sites, one at UMass, one at
Scripps. Lots of free analysis software for the PC, maybe because BD
relied on Macs for so long.
mlinde
Member
United States
11 Posts Posted - August 02 2006 : 5:44:55 PM
________________________________________
As an update, I tried trial versions of FCSexpress and FlowJo and
wasn't really happy with either of them compared to Cell Quest. I
found FCSexpress almost impossible to work with. FlowJo was alright,
but I don't think I had the time to really get the software down.
Anyone know if BD ever plans to put out CellQuest on PC?
lovesthelab
Senior Member
United States
804 Posts Posted - August 09 2006 : 1:22:04 PM
________________________________________
quote:
________________________________________
As an update, I tried trial versions of FCSexpress and FlowJo and
wasn't really happy with either of them compared to Cell Quest. I
found FCSexpress almost impossible to work with. FlowJo was alright,
but I don't think I had the time to really get the software down.
Anyone know if BD ever plans to put out CellQuest on PC?
________________________________________
Don't quote me, but I do believe that CellQuest is available for PC
because the newer BD flow cytometers are digital and use PCs for
acquisition. Mlinde, have you ever tried WinList or WinMidi? Both of
those were written for PCs. Also WEASEL comes in PC and Mac versions.
Did you read the flow perspectives? There is a software discussion
there. (Disclosure-- I wrote it).
Mlinde or anyone else, you can contact me through the SAB e-mail
system if you want to discuss flow software or anything flow some
more
off the forum. I'll be glad to help if I can.
Edited by - lovesthelab on August 09 2006 1:24:07 PM
rgrant
Moderator
Australia
2350 Posts Posted - October 10 2007 : 02:34:47 AM
________________________________________
Well, it took me about 30 seconds to discover that Mitchell Haynes is
VP Sales at Kanecki - see http://www.kanecki.com/about.html
Admin, this is blatant advertising (MH started this thread
yesterday).
--
"I don't have a lot of use for Coldplay most of the time" -- rwintle
http://network.nature.com/blogs/user/rpg
rwintle
Advanced Member
Canada
4684 Posts Posted - October 10 2007 : 10:49:46 AM
________________________________________
AND SOMEBODY TELL HIM TO STOP SHOUTING.
khenwood67
Administrator
United States
247 Posts Posted - October 10 2007 : 11:03:53 AM
________________________________________
I've deleted the topic that he started, and also deleted his post in
this forum. He won't be posting on the forums again.
Kathryn Henwood
Membership Coordinator
The Science Advisory Board
k.henw...@scienceboard.net
30 seconds to discover that Mitchell Haynes
I Useful Links & Resources - Flow Cytometry Site
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Cytometry) 46:121-135 (2001) Published ... J. Herbert Technical
Support Manager Verity Software House, Inc. PO Box 247 ...
http://www.cyto.purdue.edu/hmarchiv/2005/0579.htm - 8.0KB
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25 Mar 05
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Purdue Cytometry Mailing List: RE: FACS simulator?
... Purdue Cytometry Mailing List: RE: FACS simulator? ... Donald J.
Herbert Technical Support Manager Verity Software House, Inc. PO Box
247 45A Augusta Road Topsham, ME, USA 04086 ...
http://www.cyto.purdue.edu/hmarchiv/2005/0552.htm - 6.5KB
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23 Mar 05
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Purdue Cytometry Mailing List: FACScan for Sale
... Purdue Cytometry Mailing List: FACScan for Sale ... sale. ... The
Scan has been interfaced with a Cicero Box with Cyclops Summit
software. It has been serviced regularly since purchased in 1996. The
laser and power ...
http://www.cyto.purdue.edu/hmarchiv/2005/0024.htm - 5.6KB
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07 Jan 05
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Purdue Cytometry Mailing List: Annual Research Course in Flow Cy
... The Los Alamos National Flow Cytometry Resource (NFCR), And
Verity
Software House ... Contemporary messages sorted: [ By Date ] [ By
Thread ] [ By Subject ] [ By Author ] [ By messages with
attachments ...
http://www.cyto.purdue.edu/hmarchiv/2004/2197.htm - 7.2KB
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16 Dec 04
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Purdue Cytometry Mailing List: The Clinical Cytometry Society Cy
... Purdue Cytometry Mailing List: The Clinical Cytometry Society
Cy ... Verity Software House is proud to sponsor the Clinical
Cytometry Society Cyber Café ...
http://www.cyto.purdue.edu/hmarchiv/2004/1568.htm - 5.8KB
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02 Sep 04
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Purdue Cytometry Mailing List: Heads up for people using WinZip
... Purdue Cytometry Mailing List: Heads up for people using
WinZip ... Donald J. Herbert Technical Support Manager Verity
Software
House, Inc. PO Box 247 45A Augusta Road Topsham, ME, USA 04086
Phone ...
http://www.cyto.purdue.edu/hmarchiv/2004/1254.htm - 6.6KB
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15 Jul 04
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Purdue Cytometry Mailing List: CD8 available upon request
... Purdue Cytometry Mailing List: CD8 available upon request ...
Cytomation, Molecular Probes, Phoenix Flow Systems, Trillium
Diagnostics, Verity Software, Chroma Filters, Evergreen Laser, Tree
Star, Southern Biotechnology ...
http://www.cyto.purdue.edu/hmarchiv/2004/1178.htm - 6.5KB
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29 Jun 04
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Purdue Cytometry Mailing List: Re: FC500 files - parameter name
... read them from 3rd party software programs....so if you use the
fc500 listmode software to generate 2P histograms that ... analysis >
with WinList and notified Verity; they have added an option ...
http://www.cyto.purdue.edu/hmarchiv/2004/0582.htm - 8.2KB
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22 Mar 04
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Purdue Cytometry Mailing List: Pre-GLIIFCA DNA course
... Purdue Cytometry Mailing List: Pre-GLIIFCA DNA course ... Hi
Flowers, > FloCyte Associates and Verity Software announce a PRE-
GLIIFCA > course in DNA analysis. Want to get the best out of
your ...
http://www.cyto.purdue.edu/hmarchiv/2003/1499.htm - 4.8KB
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08 Sep 03
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Purdue Cytometry Mailing List: Pre-GLIIFCA DNA course Deadline...
... Purdue Cytometry Mailing List: Pre-GLIIFCA DNA course
Deadline... ... Hi Flowers, > FloCyte Associates and Verity Software
announce a PRE-GLIIFCA > course in DNA analysis. Want to get the best
out ...
http://www.cyto.purdue.edu/hmarchiv/2003/1500.htm - 5.1KB
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08 Sep 03
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Purdue Cytometry Mailing List: MFI calculations
... Purdue Cytometry Mailing List: MFI calculations ... The graphs
are
from Verity Software House. ...
http://www.cyto.purdue.edu/hmarchiv/2003/1439.htm - 11.4KB
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29 Aug 03
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