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ISAC XXII International Congress & Purdue Cytometry University ML &     XML Data Standards Committee Concludes New FCS 4.0 Commercial Software     Development? License Fees Could go UP!

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Mitch Haynes - 27 Aug 2008 04:35 GMT
ISAC XXII International Congress & Purdue Cytometry University ML &
XML
1993-2008 Commercial Data Storage,J Paul Robison & Crew

Purdue Cytometry Mailing List: 3rd party sw... party flow cytometry
software vendors are:

Verity Software House, Inc. 10 New ... CO 80525
800 822 9902 voice 303 226 2322 fax

TrueFacts Software, Inc. ...
www.cyto.purdue.edu/hmarchiv/1993/0046.htm - 5k - Cached - Similar
pages - Note this

Purdue Cytometry Mailing List: a tractor and other farm toolsOne
final
point on software. As some have noted in past messages, the best
software has come from independent providers

(Verity, Phoenix, TrueFacts, ...

www.cyto.purdue.edu/hmarchiv/1994/0293.htm - 7k - Cached - Similar
pages - Note this

FlowJo is Yet to come!

Will the standard be Open? Will the software be licensed? Will we be
forced to redevelop or Software.  Have they already Deveolped New
software since they have been planing to change the standard to their
own for some time now?

My client Phoenix Flow has a product, QC-Tracker, which can be
transformed into a generic FCS reader, data storage system, and user
programmable data analysis system.  Would you or others be interested

Phoenix Flow Systems
11575 Sorrento Valley Road, Suite 208
San Diego, CA 92121
619 453 5095 voice
619 259 5268 fax

In the study of economics and market competition, collusion takes
place within an industry when rival companies cooperate for their
mutual benefit. Collusion most often takes place within the market
form of oligopoly, where the decision of a few firms to collude can
significantly impact the market as a whole. Cartels are a special
case
of explicit collusion. Collusion which is not overt, on the other
hand, is known as tacit collusion.

Photonics: Principles and Practices - Google Books Resultby Abdul Al-
Azzawi - 2006 - Science - 968 pages

Newport Corporation. Optics and mechanics. Newport 1999/2000
catalog, ... Robinson. Paul, Laboratory Manual to Accompany
Conceptual
Phvsics. ...

books.google.com/books?isbn=0849382904...

Purdue Cytometry Mailing List: RE: EPICS C dataFrom: J.Paul Robinson
(j...@flowcyt.cyto.purdue.edu) ... From: Suzanne Leif > President of
Newport Instruments > Via Robert C. Leif, Ph.D. > Vice President ...

www.cyto.purdue.edu/hmarchiv/1998/1995.htm - 7k - Cached - Similar
pages - Note this

Dayong JinJingli Yuan’s group),

Newport Instruments

(Dr. Robert Leif’s group) and

Purdue University Cytometry Labs

(Prof. J.Paul Robinson’s group). ...
www.ics.mq.edu.au/gen/person/jin.html - 13k - Cached - Similar pages
-
Note this

Data and Image Analysis Special Interest Group Meeting 2007J. Paul
Robinson, SVM Professor of Cytomics, Purdue University and
President, ... (*) Robert C. Leif, Newport Instruments. "Cytometry
Standards Continuum" ...
www.ravkin.net/SBS/D&IA_SIG2007.htm - 14k - Cached - Similar pages -
Note this

[PPT] DIA SIG 2007: What are the Issues?File Format: Microsoft
Powerpoint - View as HTML
... J. Paul Robinson, President, International Society for Analytical
Cytology, ... (*) Robert C. Leif, Newport Instruments "Cytometry
Standards Continuum" ...
www.ravkin.net/SBS/DIA%20SIG%202007%20Intro.ppt - Similar pages -
Note
this

The Science Advisory Board - Protocols, Product Reviews,
Member ...Flow cytometry started as a simple instrument designed
during World War II to ... Dr. J. Paul Robinson, Purdue University
School of Veterinary Medicine, ...
www.scienceboard.net/community/perspectives.169.html - 30k - Cached -
Similar pages - Note this

Wiley Cytometry - ISAC 2000 International Congress POSTER
ABSTRACTSStephanie Ann Sincock, Purdue University; J.Paul Robinson,
Purdue University ...... Robert Leif, Newport Instruments; John
Quagliano, Los Alamos National ...
www.wiley.com/legacy/products/subject/life/cytometry/isac2000/posters...
- 223k - Cached - Similar pages - Note this

Verity Software House, Inc.
10 New Lewiston Road
Topsham, Maine 04086
207 729 6767 voice
207 729 5443 fax

Phoenix Flow Systems
11575 Sorrento Valley Road, Suite 208
San Diego, CA 92121
619 453 5095 voice
619 259 5268 fax

Cytomation, Inc.
400 E. Horsetooth Rd., Suite 100
Fort Collins, CO 80525
800 822 9902 voice
303 226 2322 fax              NEW ADDRESS FORT COLLINS DRIVE

Purdue Cytometry Mailing List: Cytomation3- Our new address-
Cytomation, Inc. 4850 Innovation Drive Fort Collins, CO 80525 4- Our
service, support and telephone availability will not change
during ...
www.cyto.purdue.edu/hmarchiv/1998/2730.htm - 7k - Cached - Similar
pages - Note this
More results from www.cyto.purdue.edu »

TrueFacts Software, Inc.
1011 Boren Ave., Suite 193
Seattle, WA 98104
800 252 5248 voice
206 621 9665 fax Robert C. Leif and Suzanne B. Leif
Ada-Med/Newport Instruments (USA)

The International Society for Analytical Cytology, ISAC, has
developed
a flow cytometry standard (FCS) to permit data interchange, ISAC will
soon replace FCS 2.0 with FCS 3.0. Unfortunately, the proposed FCS
3.0
is still fraught with problems, which are of sufficient magnitude as
to warrant its early replacement.

The most reasonable replacement is
as a supplement to the digital imaging and communications in
medicine,
DICOM 3.0, standard.

The recent digital microscopy extension of DICOM
can be extended and modified to include flow cytometry data. DICOM
includes: image graphics objects, specifications for describing:
studies, reports, the acquisition of the data and the individuals
involved, physician, patient, etc. Storing the present FCS data in a
database, which has already been accomplished with the QC tracker
software, will facilitate the transition of FCS to DICOM.

©2005 COPYRIGHT SPIE--The International Society for Optical
Engineering. Downloading of the abstract is permitted for personal
use
only.

Purdue Cytometry Mailing List: Re: Re[2]: Leave FCS3.0 alone.

My
client
Phoenix Flow has a product, QC-Tracker, which can be transformed into
a generic FCS reader, data storage system, and user programmable
data ...
www.cyto.purdue.edu/hmarchiv/1997/1762.htm - 11k - Cached - Similar
pages - Note this

From: Robert C. Leif, Ph.D. (rl...@rleif.com)
Date: Tue Jul 29 1997 - 20:25:22 EST
*       Next message: Vincent Falco: "Salary Survey Responses"
*       Previous message: Maryalice Stetler-Stevenson: "Re: CD20
Gating"
*       Maybe in reply to: Jim Houston: "Re: Re[2]: Leave FCS3.0
alone."
*       Next in thread: Dave Coder: "Re: Re[2]: Is FCS3.0 obsolete?"
*       Messages sorted by: [ date ] [ thread ] [ subject ]
[ author ]
[ attachment ]
________________________________________
To: cyto-inbox
From: Bob Leif

Please see Suzanne Leif's and my posting on the ISAC web site and
R. C. Leif and S. B. Leif, "Evolution of Flow Cytometry Standard,
FCS3.0,
into a DICOM-Compatible Format". Optical Diagnostics of Biological
Fluids
and Advanced Techniques in Analytical Cytology, Ed. A. V. Priezzhev ,
T.
Asakura, and R. C. Leif. A. Katzir Series Editor, Progress Biomedical
Optics Series , SPIE Proceedings Series,Vol. 2982, pp 354-366 (1997).

Many of your very good suggestions were separately arrived at by us.
However, there are two separate subjects: 1) What should be included
in a
Flow Cytometry File for Data Transfer and 2) The actual format for
the
Flow
Cytometry File for Data Transfer.  We suggested switching to the
Digital
Imaging and Communications in Medicine, DICOM, format after the year
2000.

My client Phoenix Flow has a product, QC-Tracker, which can be
transformed
into a generic FCS reader, data storage system, and user programmable
data
analysis system.  Would you or others be interested in this type of
product?

Purdue Cytometry Mailing List:
RE: Creating a database of FCS fiThe
product was
QCTracker from Phoenix Flow Systems.
Experience with the
development of that product was one of the reasons for the creation
of CytometryML. ...
www.cyto.purdue.edu/hmarchiv/2004/0786.htm - 8k - Cached - Similar
pages - Note this
­­­
ANYONE HAS AN EXPERIENCE TO WORK WITH WIN-FCM AND OX9 CONTROL
SOFTWARE

Purdue Cytometry Mailing List:
Phoenix Flow SystemsDoes
anyone has an
experience to work with Win-FCM Interface Box and OS9 interface
control software
from
PHOENIX Flow Systems?

We are going to get one
for ...
www.cyto.purdue.edu/hmarchiv/1997/0271.htm - 5k - Cached - Similar
pages - Note this
More results from www.cyto.purdue.edu »

Penn SDM Flow Cytometry Facility

Flow Cytometry at Purdue University;
The Purdue links are excellent starting ...
BrdU-based TUNEL Assay
from
Phoenix Flow plus a Cell Fixation Procedure ...

Map of 4010 Locust St, Philadelphia, PA 19104

biochem.dental.upenn.edu/~sdmfacs/ - 14k - Cached - Similar pages -
Note this

(Cytomation, Coulter, Verity, Phoenix Flow, and MSA, not B-D)
NOT
B-D

Purdue Cytometry Mailing List: Re: Histogram SubtractionThis method
is available in most
commercial flow software
(Cytomation, Coulter,
Verity, Phoenix Flow, and MSA, not B-D).
In the years since then, ...
www.cyto.purdue.edu/hmarchiv/1998/1740.htm - 8k - Cached - Similar
pages - Note this

[PDF] Great Lakes International Imaging and Flow Cytometry
AssociationFile
Format: PDF/Adobe Acrobat -
View as HTML

Cytomation, Inc. DAKO Corporation.

Flow Cytometry Standards Corporation ...
Karen Cornell,
Purdue University, West Lafayette, IN,
“Flow. Cytometric and ...
www.gliifca.org/pdf/GLIIFCA-1995.pdf - Similar pages - Note this

THANKS FOR A SUCCESSFUL 2007

Purdue Cytometry Mail list-Cytometry Education Associates, Inc ...NW
Regional ... ... Purdue Cytometry Mailing List: RE: Thanks to all for
a successful 2007 NW Regional ... Miltenyi, Phoenix Flow Systems,
Spherotech, Tree ...
groups.google.co.uk/group/misc.health.aids/browse_thread/thread/
a7d391d007a402e7 - 179k - Cached - Similar pages - Note this
More results from groups.google.co.uk »

From: Robert C. Leif <rl...@rleif.com>The FCS header files have
already been parsed and stored in a database(1). The product was
QCTracker from Phoenix Flow Systems.

RE: Creating a database of FCS files

   * This message: [ Message body ] [ More options ]
   * Related messages: [ Next message ] [ Previous message ] [ In
reply to ]

From: Robert C. Leif <rl...@rleif.com>
Date: Sat Apr 24 2004 - 11:57:17 EST

The FCS header files have already been parsed and stored in a
database(1).
The product was QCTracker from Phoenix Flow Systems.

Experience with the development of that product was one of the
reasons
for
the creation of CytometryML. Since the data in a CytometryML file is
all
validated XML, it can be imported without any changes into
commercially
available databases, spreadsheets and other applications.  The list
mode
data and associated index files are stored as a simple array of
records(structs), which can be read by commercially available common
programming languages or manipulated by .Net object.

1. R. C. Leif, R. Rios, M. C. Becker, C. K. Becker, J. T. Self, and
S.
B.
Leif, "The Creation of a Laboratory Instrument Quality Monitoring
System
with AdaSAGE". Advanced Techniques in Analytical Cytology, Optical
Diagnosis
of Living Cells and Biofluids, Ed. T. Askura, D. L. Farkas, R. C.
Leif, A.
V. Priezzhev, , and B. J. Tromberg.. A. Katzir Progress in Biomedical
Optics
Series Editor SPIE Proceedings Series, Vol. 2678, 232-239 (1996).

2. R. C. Leif, S. B. Leif, and S. H. Leif, "CytometryML, An XML
Format
based
on DICOM for Analytical Cytology Data ", Cytometry 54A pp. 56-65
(2003).

3. R.C. Leif, S.H. Leif, S.B. Leif, CytometryML, a markup language
for
analytical cytology, in Manipulation and Analysis of Biomolecules,
Cells and
Tissues, D. V. Nicolau, J. Enderlein, and R. C. Leif, Editors, SPIE
Proceedings Vol. 4962 pp 288-297 (2003).

-----Original Message-----
From: Adrian Smith [mailto:A.Sm...@centenary.usyd.edu.au]
Sent: Thursday, April 22, 2004 6:57 PM
To: cyto-inbox
Subject: Creating a database of FCS files

Hi all,

Some of the users here have raised the desirability of having a
database of all the FCS headers from all their data files. They could
then, for example, search for all the files/experiments in which they
used a particular stain etc.

Is anybody doing this? Would this be something that other people
would find useful?

I would love to set something up but I don't have the requisite
skills or time at the moment.

As a temporary measure I suggested they export the FCS header info
from FlowJo using using a table and then compile them all in another
program like excel. This works for a few experiments but it needs to
be automated (and easy) if it is going to be generally applicable.

Any comments or suggestions?

Adrian Smith
Centenary Institute of Cancer Medicine and Cell Biology
Sydney, Australia

Received on Mon Apr 26 14:38:00 2004

   * This message: [ Message body ]
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recombinant IL-6"
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Cytometry"
   * In reply to: Adrian Smith: "Creating a database of FCS files"

   * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
Subject ] [ By Author ] [ By messages with attachments ]

This archive was generated by hypermail 2.1.8 : Tue Apr 27 2004 -
03:12:02 EST

RE: Phoenix software for Coulter analyser
From: Dan Smith (D...@ALLP.COM)
Date: Thu Jan 30 1997 - 16:48:00 EST
•     Next message: Pizzo,Eugene: "FACSCalibur 4-color option"
•     Previous message: Pete Macardle: "(no subject)"
•     Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
Phoenix Flow's multiplus has worked very well in analyzing Profile II
data
for us.
Dan Smith
San Diego
----------
{From: owner-cyto-sendout
{To: Cytometry Mailing List
{Subject: Phoenix sofware for Coulter analyser
{Date: Thursday, January 30, 1997 4:28PM
{
{
{We are looking into software for good display and analysis of data.
{We use a coulter profile 2 analyser. Does anyone have experience
with
{Phoenix MultiPlus analysis software, or could recommend any other
{software package for consideration?
{
{I would appreciate any advice.
{
{Stan.Stan Ress
{Clinical Immunology laboratory
{Dept of Medicine
{H47 Old main BLG,
{Groote Schuur Hospital,
{Observatory,
{Cape Town, South Africa
{7925
{
{e-mail:  sr...@uctgsh1.uct.ac.za
{Phone:   Intern + 2721-4066201
{FAX  :   Intern + 2721-4486815
{
________________________________________
•     Next message: Pizzo,Eugene: "FACSCalibur 4-color option"
•     Previous message: Pete Macardle: "(no subject)"
•     Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
17:33:51 EST

From: Robert C. Leif, Ph.D. (rl...@rleif.com)
Date: Tue Jul 29 1997 - 20:25:22 EST
*       Next message: Vincent Falco: "Salary Survey Responses"
*       Previous message: Maryalice Stetler-Stevenson: "Re: CD20
Gating"
*       Maybe in reply to: Jim Houston: "Re: Re[2]: Leave FCS3.0
alone."
*       Next in thread: Dave Coder: "Re: Re[2]: Is FCS3.0 obsolete?"
*       Messages sorted by: [ date ] [ thread ] [ subject ]
[ author ]
[ attachment ]
________________________________________
To: cyto-inbox
From: Bob Leif

Please see Suzanne Leif's and my posting on the ISAC web site and
R. C. Leif and S. B. Leif, "Evolution of Flow Cytometry Standard,
FCS3.0,
into a DICOM-Compatible Format". Optical Diagnostics of Biological
Fluids
and Advanced Techniques in Analytical Cytology, Ed. A. V. Priezzhev ,
T.
Asakura, and R. C. Leif. A. Katzir Series Editor, Progress Biomedical
Optics Series , SPIE Proceedings Series,Vol. 2982, pp 354-366 (1997).

Many of your very good suggestions were separately arrived at by us.
However, there are two separate subjects: 1) What should be included
in a
Flow Cytometry File for Data Transfer and 2) The actual format for
the
Flow
Cytometry File for Data Transfer.  We suggested switching to the
Digital
Imaging and Communications in Medicine, DICOM, format after the year
2000.

My client Phoenix Flow has a product, QC-Tracker, which can be
transformed
into a generic FCS reader, data storage system, and user programmable
data
analysis system.  Would you or others be interested

Purdue Cytometry Mailing List: Cytomation3- Our new address-
Cytomation, Inc. 4850 Innovation Drive Fort Collins, CO 80525 4- Our
service, support and telephone availability will not change
during ...
www.cyto.purdue.edu/hmarchiv/1998/2730.htm - 7k - Cached - Similar
pages - Note this
More results from www.cyto.purdue.edu »

Cytomation Inc.'s CyCLOPS softare. Data acquired on the Coulter
Epics ..... Purdue University Cytometry Laboratory.

Cytomation Inc.'s CyCLOPS softare. Data acquired on the Coulter
Epics ..... Purdue University Cytometry Laboratory.

[PDF] MFI: a flow cytometry list mode data analysis program
optimized ...File Format: PDF/Adobe Acrobat - View as HTML
Cytomation Inc.'s CyCLOPS softare. Data acquired on the Coulter
Epics ..... Purdue University Cytometry Laboratory. I am grateful to
the National Science ...
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Purdue Cytometry WinMDI software Fails on LM-files contact
Verity ...Purdue Cytometry WinMDI software Fails on LM-files contact
Verity House Software .... interpret CyCLOPS files provided by
Cytomation, Inc. (303-226-2200, ...

groups.google.com/group/misc.health.aids/browse_thread/thread/
7410e3c6def72b16 - 51k -

The Wistar Institute :: Flow Cytometry FacilityBasics of High-Speed
Cell Sorting on the Cytomation MoFlo .... Samples were analyzed using
a DakoCytomation MoFlo (DakoCytomation, Inc., Fort Collins, CO) ...
www.wistar.org/research_facilities/facilities/flowcytometry/links.html
- 31k - Cached - Similar pages - Note this

____________________________The Science Advisory Board - Protocols,
Product Reviews, Member ...- 57 visits - Jun 6Dr. J. Paul Robinson,
Purdue University School of Veterinary Medicine, .... Beckman-
Coulter,
or Cytomation (Dako) instruments- check the website for the ...
www.scienceboard.net/community/perspectives.169.html - 30k - Cached -
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Purdue Frederick Co. under the trademark "ALFERON" (Norwalk, ......
When using the preferred flow cytometer and software, the FACScan
running the ...
www.wipo.int/pctdb/en/wo.jsp?IA=WO1997019189&DISPLAY=DESC - 167k -

iCyt - The Cell Sorter and Flow Cytometer Company - ManagementAs the
first manager of the Purdue University Flow Cytometry Facility
(PUCL), ... Fredrick Molnar is the Chief Sales, Marketing and Service
Officer of iCyt. ...
www.i-cyt.com/about/management.htm - 9k - Cached - Similar pages -
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PDF] CURRENT PROTOCOLS IN CYTOMETRYFile Format: PDF/Adobe Acrobat -
View as HTML
CYTOMETRY. EDITORIALBOARD. J. Paul Robinson, Managing Editor. Purdue
University ...... CYTOPC: Processing software for. flow cytometric
data. ...
www.sb-roscoff.fr/Phyto/Reprints/Marie_et_al_CPC_00.pdf - Similar
pages - Note this

[PDF] CURRENT PROTOCOLS IN CYTOMETRYFile Format: PDF/Adobe Acrobat -
View as HTML
Purdue University. West Lafayette, Indiana. Zbigniew
Darzynkiewicz ...... CYTOPC: Processing software for. flow cytometric
data. Signal Noise 2:8. ...
www.sb-roscoff.fr/Phyto/Reprints/Marie_et_al_CPC_99.pdf - Similar
pages - Note this

Great Lakes International Imaging and Flow Cytometry Association
File Format: PDF/Adobe Acrobat - View as HTML
Karen Cornell, Purdue University, West Lafayette, IN, “Flow.
Cytometric and Image Analysis of ... Abe Schwartz, Flow Cytometry
Standards Corp., San Juan, ...
www.gliifca.org/pdf/GLIIFCA-1995.pdf - Similar pages - Note this

Bangs Laboratories, Inc. :: Microspheres
Flow Cytometry Standards Corporation Joins Bangs Laboratories to
Provide a Full ... May 2000, Fishers, IN -- Flow Cytometry Standards
Corporation has joined ...
www.bangslabs.com/company/press.php?whichrelease=5 - 11k - Cached -
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Commercial Flow Cytometry Links
Last Updated: 2006-09-07 See also: Salk Flow Cytometry Lab Bioscience
Links. ... Flow Cytometry Standards Corp, San Juan, PR, [Acquired by
Bangs Labs] ...
flowcyt.salk.edu/comflow.html - 14k - Cached - Similar pages - Note
this

Development of clinical standards for flow cytometry -- Schwartz ...
Flow Cytometry Standards Corporation, Research Triangle Park, North
Carolina 27709. Flow cytometers are instruments that can determine
multiparameter data ...
www.annalsnyas.org/cgi/content/abstract/677/1/28 - Similar pages -
Note this

Flow cytometry
Kina, and J. Paul Robinson, Purdue University Cytometry Laboratories
and .... Stelzert and C. Stewart**, Flow Cytometry Standards
Corporation*, ...
doi.wiley.com/10.1002/cyto.990140907 - Similar pages - Note this

[PPT] A Unique Opportunity in Biological Information Standards C.
Forbes ...File Format: Microsoft Powerpoint - View as HTML

Flow Cytometry / FACS; Microarray Experiments; Mass Spectrometry;
Microscope Images ... --Robert C. Leif, Suzanne B. Leif, et al.,
XML_Med, a Division of ...
www.w3.org/2004/10/swls/forbes_dewey.ppt - Similar pages - Note this

Increasing the luminescence of lanthanide complexesment Funds, Lidia
Vallarino’s Gift Fund,

and Phoenix Flow Systems Internal ...... 66. Leif RC. CytometryML
binary data standards. Proc SPIE 2005;5699: .

..
doi.wiley.com/10.1002/cyto.a.20321 - Similar pages - Note this
More results from doi.wiley.com »

[PDF] Increasing the Luminescence of Lanthanide(III) Macrocyclic
ComplexesFile Format: PDF/Adobe Acrobat - View as HTML
S.A. Williams. c. , and S. Yang. a. a. Newport Instruments, San
Diego,
CA 92115-1022, USA. b. Phoenix Flow Systems, San Diego, CA
92121-1319 ..

.
www7.nationalacademies.org/bpa/COHMAG_Input_Leif2.pdf - Similar pages
- Note this

by RC Leif - Cited by 3 - Related articles - All 9 versions

Defense Technical Information Center Compilation Part NoticeR. C.
Leif, Methods for Preparing Sorted Cells as Monolayer Specimens, In
Living Color, Protocols in Flow Cytometry and Cell. Sorting, R. A.
Diamond and S. ...
www.stormingmedia.us/68/6889/P688910.pdf - Similar pages - Note this
by RC Leif - 2004

[PDF] CytometryML, a markup language for analytical cytologyFile
Format: PDF/Adobe Acrobat - View as HTML

XML_Med, a Division of Newport Instruments, 5

648 Toyon Road, San Diego, ...... R. C. Leif and S. B. Leif,
"Evolution of Flow Cytometry Standard, FCS3.0, ...
chemdye.com/cytometryml/pdf/CytometryML_SPIE03.pdf - Similar pages -
Note this

[PDF] Nov2005: Abstracts: George L. Wied Memorial SymposiumFile
Format: PDF/Adobe Acrobat

Robert Leif, Ph.D., P.M.I.A.C.. Objective: Cytology automation will
be
enhanced by the ... croscopy and flow cytometry can be created in a
manner ...

www.acta-cytol.com/admin/wiedsymposiumabs.pdf - Similar pages - Note
this

Reagent system and method for modifying the luminescence of ...(k) A
pair of Leif Centrifugal Cytology Buckets (Ref. 33)

(Newport Instruments) that ..... 35) described in the

Phoenix Flow Systems ABSOLUTE-S™ Kit (Ref. ...

www.freepatentsonline.com/y2007/0134160.html - Similar pages - Note
this

(WO/2005/046735) A REAGENT SYSTEM AND METHOD FOR MODIFYING THE ...(k)
A pair of Leif Centrifugal Cytology Buckets (Ref. 33) (Newport
Instruments) that ..... Parallel flow cytometry measure- ments with
fluorescein labeled ...
www.wipo.int/pctdb/en/wo.jsp?IA=US2004037314&DISPLAY=DESC - 841k -
Cached - Similar pages - Note this

Wiley Cytometry - ISAC 2000 International Congress POSTER
ABSTRACTSStephanie Ann Sincock,

Purdue University; J.Paul Robinson, Purdue University ...... Robert
Leif, Newport

Instruments; John Quagliano, Los Alamos National ...
www.wiley.com/legacy/products/subject/life/cytometry/isac2000/posters...

ISAC XXII International CongressPhoenix Flow Systems, Newport
Instruments R&D, San Diego, ...... J. Paul Robinson. 6. 1. Purdue
University, Electrical and Computing Engineering, West ...
doi.wiley.com/10.1002/cyto.a.20045.abs - Similar pages - Note this
by P Session

ISAC XXII International CongressPhoenix Flow Systems, Newport
Instruments

Data and Image Analysis   Special Interest Group Meeting 2007

     J. Paul Robinson, SVM Professor of Cytomics,

Purdue University and President, ... (*) Robert C. Leif, Newport
Instruments.

Cytometry Standards Continuum" ...

www.ravkin.net/SBS/D&IA_SIG2007.htm - 14k - Cached - Similar pages -
Note this

AbstractsSilas J. Leavesley1,

Bartlomiej Rajwa2,

J. Paul Robinson3 1Purdue ...... R&D, San Diego, California;

2Phoenix Flow Systems,

Newport Instruments R&D, ...

www.cyto.purdue.edu/flowcyt/isac2006/abstracts.htm - 977k - Cached -
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[PDF] Call for Papers

File Format: PDF/Adobe Acrobat - View as HTML
Sinai Medical Ctr.; Dan V. Nicolau, The Univ. of Liverpool (United
Kingdom);

Robert C. Leif,. Newport Instruments.
Cochairs: J. Paul Robinson, Purdue Univ.; ...

spie.org/Documents/ConferencesExhibitions/Photonics-West-2009-BiOS-
Call.pdf - Similar pages - Note this

Flow cytometry

Kina, and J. Paul Robinson,

Purdue University Cytometry Laboratories

and ....

Stelzert and C. Stewart**,

Flow Cytometry Standards Corporation*, ...

doi.wiley.com/10.1002/cyto.990140907 - Similar pages - Note this

[PDF]

MFI: a flow cytometry list mode data analysis program optimized ...
File Format: PDF/Adobe Acrobat - View as HTML

(#3, Flow Cytometry Standards Corp., POB 12621,

Research Triangle Park .....

Purdue University Cytometry Laboratory.\

I am grateful to the National Science ...

www.umass.edu/microbio/mfi/mfims.pdf - Similar pages - Note this

Commercial Flow Cytometry Links

Last Updated: 2006-09-07 See also:

Salk Flow Cytometry Lab Bioscience Links. ..

. Flow Cytometry Standards Corp

, San Juan, PR, [Acquired by Bangs Labs] ...

flowcyt.salk.edu/comflow.html - 14k - Cached - Similar pages - Note
this

Purdue Cytometry Mailing List: RE: Report from

ISAC Meeting San Diego

Thanks to Phoenix Flow Systems,

Guava Technologies,

PROzyme and Becton ...

Adam Treister Tree Star, Inc.

ph: 800-366-6045 intl: 1-650-591-2854 fax: ...

Address any questions, comments, or suggestions to

Mario Roederer.

www.cyto.purdue.edu/hmarchiv/2002/1065.htm - 8k - Cached - Similar
pages - Note this

Sunday, July 13, 2008
ISAC Congress Biological Councilor 2008 has UR FlowJo Month
ISAC CONGRESS Biological Councilor 2008 has UR FlowJo Month

Highlights of the XXII 2004 ISAC Congress

Thanks from the Coalition for Change team!

6 of the 7 members of our team are now

on ISAC Council,

including the new

President Elect (J.Paul Robinson)
&

Secretary (Bob Zucker).

Data Standards Committee!

Apple - Science - Profiles - Dr. Mario Roederer
Dr. Mario Roederer. In a major new revision for Mac OS X, FlowJo 6
brings ... Roederer and Treister developed FlowJo for the Mac first
because one of the ...www.apple.com/science/profiles/roederer/ - 17k -
Cached - Similar pages - Note this

Bushnell, Timothy Mambo Mar 6, 2008 ISAC Election Biological
Councilor.

Disclaimer: from the Purdue Cytometry Mail List ARCHIVE

Yes, I live off FlowJo sales, and that's a blatantly commercial
statement, but it gets technical from here on.]

http://www.cyto.purdue.edu/hmarchiv/2006... - 8.5KB

On Aug 20, 2004, at 2:25 PM,

J. Paul Robinson wrote:

ummm....Mario says.. "In life sciences -- particularly in research
life sciences --> probably 50 to 70% of research laboratories used
Macs"....
while I have a passionate dislike for Windows......
is this really true ??? or is the key word there "used"??

(Ok...I have put on my helmet and armor....waiting...) paul

For all of the mr groupies out there in cytometry cyberspace.
Don't wet your pocket protectors over this.

Honestly though, well deserved praise for Mario & the Tree Star group

http://www.apple.com/science/profiles/ro...

FlowJo MONTH at UR CPBR Flow Lab University of Rochester Office.

October 2007 Options:Show Author Hide Author Show Table of Contents
Hide Table of Contents Join or Leave UR_CYTOMETRYSearch
ArchivesSubject

From Date Size2nd call - Fall Flowjo order Bushnell, Timothy

Tue, 9 Oct 2007 09:30:09 -0400309 linesFall Flowjo order Bushnell,
Timothy

Mon, 1 Oct 2007 09:30:27 -0400242 linesFlocyte regional training
program Bushnell, Timothy

Wed, 31 Oct 2007 14:30:22 -0400221 linesLast call - Fall Flowjo order
Bushnell, Timothy

Thu, 25 Oct 2007 08:15:20 -0400231 linesReminder: 1 pm today: Flow
Cytometry Lecture Series. Bushnell, Timothy

Thu, 11 Oct 2007 09:19:48 -0400214 linesToday is the last day to join
the Flowjo bulk order Bushnell, Timothy

Tue, 23 Oct 2007 09:23:59 -0400341 linesBack to:

Main UR_CYTOMETRY Page

Bushnell, Timothy Date:Fri, 11 Jan 2008 10:31:11 -0500

Colleagues:
Dr. John Quinn, applications scientist from Treestar, will be
presenting web based seminar Friday January 11th at 1 pm on KMRB
3-9624 on Compensation and Transformation All are welcome.
Regards
Tim Timothy Bushnell, Ph.D.Research Assistant Professor, Pediatrics
and Oncology
Co-Director, URMC Flow Cytometry FacilityOffice: 585-273-5535Lab:
585-273-1361Fax: 585-276-0233http://www.urmc.rochester.edu/Aab/
genepe...

Subject:Reminder: Today 1 pm - Flowjo Seminar on Compensation and
Transformation
From:"Bushnell, Timothy" October 2007

Thanks > > Tim > > > > Timothy Bushnell, Ph.D. > > Research Assistant
Professor, ... Justin, We're working hard to finalize FlowJo
Collectors' Edition, ...mycyte.org/index.php?option=com_newsfe... -
133k - Cached - Similar pages -
Tree StarFile Format: PDF/Adobe Acrobat - View as HTMLData Analysis/
Presentation – Tim Bushnell & Ryan Duggan ..... Purdue University
Cytometry Laboratories, West Lafayette, IN ...www.gliifca.org/pdf/
GLIIFCA-2004.pdf -

Purdue Cytometry Mailing List: FW: Second Announcment:WNYFUG 2
From : Bushnell, Timothy ... is sponsored by Treestar, and the best
poster will win a 1 year subscription to
Flowjo! ...www.cyto.purdue.edu/hmarchiv/2006/1019... - 9k - Cached -
Similar pages -

Purdue Cytometry Mailing List:
By DateRE: FCS files to Excel with Diva or FlowJo Ashley Carter.
Thu Mar 13 2008 - 18:25:41 EDT ..... New email list for Imagestream
users Bushnell, Timothy ...www.cyto.purdue.edu/hmarchiv/Current/ -
248k - Cached - Similar pages - Note thisMore results from www.cyto.purdue.edu
»

Flowjo purchase 2007
From:"Bushnell, Timothy"
Reply-To:Cytometry
Date:Tue, 3 Apr 2007 14:55:23 -0400
Colleagues:
As you know, I have organized a yearly purchase for Flowjo, a flow
cytometry data analysis package. If you are not familiar with this
software, you can find information at http://www.flowjo.com/home/overview.html...
A single seat (dongle/license) is $1325. We start to get additional
discounts when we order more than 5 dongles.

I have been approached by several labs interested in purchasing new
copies of the software, so I am starting to organize another bulk
purchase for this spring. If your lab is interested please email me
privately with the number of dongles you wish to purchase and an
account number to charge the purchase.

I will start the paperwork on 16 April, so please get your information
to 1 day ago me promptly.
Regards,
Tim Timothy Bushnell, Ph.D.Research Assistant Professor, Pediatrics
and OncologyDirector, CPBR Flow LabUniversity of Rochester

http://www.urmc.rochester.edu/Aab/genepe...
____UR_Cytometry______Subscribers Corner_____ http://listserv.urmc.rochester.edu

Subject:Fall Flowjo order

From:"Bushnell, Timothy"
Reply-To:Cytometry
Bushnell, Timothy
Date:Mon, 1 Oct 2007 09:30:27-0400

Colleagues:
As in the past, I am organizing a bulk order for copies of the flow
cytometry analysis software package Flowjo, produced by Treestar,
Inc.
You can find details on this software are www.flowjo.com. The base
cost will be $1325 per copy + shipping ($18/#copies ordered).

I can usually arrange additional discounts when we order greater than
5 copies. If you are interested, please send me the number of copies
you are interested in, the PI’s name, and an account number before
October 15th.

Regards
Tim Timothy Bushnell, Ph.D.Research Assistant Professor, Pediatrics
and OncologyDirector, CPBR Flow LabUniversity

ISAC E-News -- June, 2007 - Mambo
Jun 8, 2007 ... You also may contact the President, Dr. Tim
Bushnell, ... Adam Treister introduced possibilities of integrating
these with FlowJo. ...www.isac-net.org/content/view/593/119/ - 53k -
Cached - Similar pages - Note this

ISAC E-NewsFile Format: PDF/Adobe Acrobat - View as HTMLthe President,
Dr. Tim Bushnell, at Timothy_Bushnell@URMC.rochester.edu. ...
introduced possibilities of integrating these with
FlowJo. ...www.zangani.com/files/2007-0608-ISAC_E... - Similar pages -
Note this

Subject:Reminder: Spring Flowjo order
From:"Bushnell, Timothy"
Date:Fri, 14 Mar 2008 12:21:24 -0400
Colleagues:
Is your lab in need of a new flowjo dongle?
I am again coordinating a bulk purchase of flowjo dongles.
The academic price is $1495 a dongle.
In the past, the price breaks start at 3 dongles and go up from
there.

If your lab is interested in purchasing a dongle, please email me
directly the name of the PI, an account number to charge, and the
number of dongles you wish to purchase by March 17th.

Tim Timothy Bushnell, Ph.D.Research Assistant Professor, Pediatrics
and OncologyCo-Director, URMC Flow Cytometry

Purdue Cytometry Mail list-Cytometry Education Associates, Inc-Verity
Software House, Inc___a non-profit umbrella organization....http://
www.vsh­.com/cytometrycourses/

Los Alamos National Laboratory helps to ensure the safety of the
nuclear ... the National Flow Cytometry Resource (n...@lanl.gov) and
Verity Software House. ...
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NFCR Training, Service, and Dissemination
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Contact Gary Salzman, Los Alamos National Laboratory, Los Alamos NM
USA. ... Alternative transfer software sold by Verity, which uses the
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Verity Software House, Inc. and. ROSWELL PARK CANCER INSTITUTE.
BUFFALO, NEW YORK .... James Jett, Los Alamos National Laboratory,
Los
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LosAlamos,N ew M e ×icpB7545
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Verity Software House has a variety of. _ programs for analysis for
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Los
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who holds ... Los Angeles and the National Institutes of Health were
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Proposed New Data File Standard for Flow Cytometry, Version FCS 3.0
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Los Alamos National Laboratory, Los Alamos, New Mexico. 3. Verity
Software House, Topsham, Maine. 4. National Institutes of Health,
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ver...@vsh.com ... Los Alamos National Laboratory. Mail Stop M888.
Los
Alamos, New Mexico 87545 ...
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Verity Software House, Inc., Topsham, Maine, 04086 (C.B.B.);
Biophysics Group, Division ... National Laboratory,. Los. Alamos, New
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an
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APS Model
File Format: Microsoft Powerpoint - View as HTML
Los Alamos National Laboratory. Research Library. LITA Forum 2003 ...
Software components. Verity search engine; MySQL to handle author
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Software and online help: WinMDI and other software ... Photo
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Raman Spectroscopy Detects Biochemical Changes Due to ...
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fractions of ...
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BIOGRAPHICAL SKETCH Hawley (nee Lam), Teresa S. Director, Flow ...
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2003 - 06 Beta-testing Site for Verity Software House (WinList3D
v5.0/6.0 pre-releases) ... Alamos National Laboratory, Los Alamos, NM
(June 2005) ...
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Deep Web Technologies - Company Information - Management
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Verity Software House. (207) 729-6767 x102. c...@vsh.com. (207)
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Biochemical and Biophysical Research Communications : Inhibition ...
d Los Alamos National Laboratories, Biosciences Division, Los Alamos,
NM 87545, USA .... profile was analyzed using Modifit's Sync Wizard
(Verity Software). ...
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- Note this
InfoWorld: Product Guide: Verity TeleForm 9.0 : Company
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technology. At Los Alamos National Laboratory he developed a number
of
the first web-based ...
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PUCL - Purdue University Cytometry Laboratories - Introduces ...
Purdue Cytometry Mailing List: Annual Research Course in Flow Cy ...
The Los Alamos National Flow Cytometry Resource (NFCR), And Verity
Software House . ...
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Wake up people - times are changing - look at all these new small
companies trying to stick their noses in "our" field! Wake up people
-
times are changing - look at all these new small companies trying to
stick their noses in "our" field! Wake up people - times are changing
- look at all these new small companies trying to stick their noses
in
"our" field! Purdue Cytometry Mailing List: Re: 2008 NW Regional
Cytometry Meeting, March 13 - 15 in Portland ... Technology,
IntelliCyt, Miltenyi Biotec, Partec, StemCell Technologies, Tree
Star,
Union Biometrica, and Verity Software House. ...
http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1946.htm - 6.9KB 74%
|||||||||||||||||||| 09 Dec 07 Find Similar Highlight Purdue
Cytometry
Mailing List: New England Cytometry Users Group Meeting - Thanks! ...
Purdue Cytometry Mailing List: New England Cytometry Users Group
Meeting - Thanks! ... C. Bruce Bagwell, MD, Ph.D. - Verity Software
House ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1593.htm -
7.1KB 74% |||||||||||||||||||| 25 Oct 07 Find Similar Highlight
Purdue
Cytometry Mailing List: New England Cytometry Users Group annual
meeting reminder ... Purdue Cytometry Mailing List: New England
Cytometry Users Group ... Bruce Bagwell, MD, Ph.D. - Verity Software
House "Probability State Models: A ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1546.htm
- 7.4KB 74% |||||||||||||||||||| 17 Oct 07 Find Similar Highlight
Purdue Cytometry Mailing List: New England Cytometry Users Group
annual meeting reminder ... Purdue Cytometry Mailing List: New
England
Cytometry Users Group ... Bruce Bagwell, MD, Ph.D. - Verity Software
House "Probability State Models: A ... http://www.cyto.purdue.edu/hmarchiv/current/1546.htm

- 7.4KB 74% |||||||||||||||||||| 17 Oct 07 Find Similar Highlight
Document count: purdue (139318) cytometry (25468) mail (53709) list
(63448) 2007 (37553) verity (222) software (15216) sales (14402) 07
(21574) by (129519) thread (26375) purdue cytometry mail list 2007
verity software sales 07 by... (27962) about 187244 results found,
top
500 sorted by relevance score using date hide summaries group by
location spacer 1-10 next

Purdue Cytometry Mailing List: RE: DNA
analysis software ... Purdue Cytometry Mailing List: RE: DNA analysis
software ... J. Herbert Technical Support Manager Verity Software
House ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0747.htm -
7.7KB 78% |||||||||||||||||||| 26 May 07 Find Similar Highlight
Purdue
Cytometry Mailing List: Re: DNA analysis software ... vsh.com] >
Sent:
Wednesday, February 21, 2007 10:30 AM >

To: Cytometry Mailing List >
Subject: RE: DNA ... J. Herbert
Technical Support Manager
Verity Software House ... http://www.cyto.purdue.edu/hmarchiv/Current/0755.htm
- 8.6KB 78% |||||||||||||||||||| 26 May 07 Find Similar Highlight

Purdue Cytometry Mailing List: Announcing User Forum at Verity

Software House, Inc. ...

Purdue Cytometry Mailing List: Announcing
User Forum at Verity ... Verity Software House is pleased to announce
the creation of a Verity User's Forum on our ...
http://www.cyto.purdue.edu/hmarchiv/2002/0542.htm - 4.6KB 78%
|||||||||||||||||||| 12 Mar 02 Find Similar Highlight Purdue
Cytometry
Mailing List: RE: FL2H in PI cell cycle analysis ... From: Verity
Software House Date: Fri Sep 07 2007 - 17:30:59 EDT ... From: Verity
Software House Date: Fri Sep 07 2007 - 17:30:59 EDT ...
http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1351.htm - 7.2KB 77%
|||||||||||||||||||| 12 Sep 07 Find Similar Highlight

Purdue Cytometry Mailing List: By Date ... Purdue Cytometry Mailing
List By Thread ...
Jim (NIH/NIAMS) (Wed Dec 19 2007 - 13:41:51 EST) ... 13th Leipziger
Workshop, 2nd Call and Seasonal Greetings Dr. Attila Tarnok (Wed Dec
19 2007 - 07:41:10 EST) ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/thread.htm
- 328.1KB 76% |||||||||||||||||||| 21 Dec 07 Find Similar Highlight

Purdue Cytometry Mailing List: DNA analysis software ... Purdue
Cytometry Mailing List: DNA analysis software ... we offer a
conversion at no charge. I invite you to contact Verity Software
House
directly with any specific questions or issues.
Best regards, Don
Donald ...
http://www.cyto.purdue.edu/hmarchiv/Current/0753.htm -
5.6KB 76% |||||||||||||||||||| 26 May 07 Find Similar Highlight
Purdue
Cytometry Mailing List: By Date ... Purdue Cytometry Mailing List: By
Date ... Wed Dec 19 2007 - 07:41:10 EST ... http://www.cyto.purdue.edu/hmarchiv/Current/
- 362.4KB 75% |||||||||||||||||||| 21 Dec 07 Find Similar Highlight
Purdue Cytometry Mailing List: By Date ... Purdue Cytometry Mailing
List: By Date ... Mon Sep 24 2007 - 22:25:06 EDT) ... Re: mCherry on
a
Calibur ??!! (Thu Aug 30 2007 - 19:36:32 EDT) ... Re: DiI and FITC on
a Vantage (Tue Aug 07 2007 - 20:07:02 EDT) ...
http://flowcyt.cyto.purdue.edu/hmarchiv/Current/author.htm - 300.2KB
75% |||||||||||||||||||| 21 Dec 07 Find Similar Highlight Purdue
Cytometry Mailing List: By Date ... Purdue Cytometry Mailing List: By
Date ... Derek Davies (Wed Mar 07 2007 - 07:56:30 EST) ...
http://flowcyt.cyto.purdue.edu/hmarchiv/Current/subject.htm - 289.4KB
75% |||||||||||||||||||| 21 Dec 07 Find Similar Highlight

Purdue Cytometry Mailing List: Cytometry software for linux ...
Purdue
Cytometry Mailing List: Cytometry software for linux ... Previous
message: Verity Software House: "RE: FL2H in PI cell cycle
analysis" ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1352.htm
- 5.5KB 75% |||||||||||||||||||| 13 Sep 07 Find Similar about 187243
results found, top 500 sorted by relevance score using date hide
summaries group by location prev 21-30 next Purdue Cytometry Mailing
List: Re: FL2H in PI cell cycle analysis ... Purdue Cytometry Mailing
List: Re: FL2H in PI cell cycle analysis ... Next in thread: Verity
Software House: "RE: FL2H in PI cell cycle analysis" ...
http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1336.htm - 6.6KB 74%
|||||||||||||||||||| 11 Sep 07 Find Similar Highlight Purdue
Cytometry
Mailing List: New England Cytometry Users Group annual meeting
preliminary ... ...

Purdue Cytometry Mailing List: New England
Cytometry Users Group annual meeting preliminary announcement ... up
of speakers this year: C. Bruce Bagwell, MD, Ph.D. - Verity Software
House http://www.vsh.com/ Anne E. Carpenter, Ph.D. - MIT ...
http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1153.htm - 7.2KB 74%
|||||||||||||||||||| 08 Aug 07 Find Similar Highlight Purdue
Cytometry
Mailing List: An innovative new two DVD set on Cytometry- Free to
all ... John Wiley & Sons, publisher of Cytometry Part A. These major
players in the field of cytometry are known to all of ... Scientific,
Coherent, Q-Imaging and Verity Software. All of these companies
provide ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0805.htm
-
8.7KB 74% |||||||||||||||||||| 07 Jun 07 Find Similar Highlight
Purdue
Cytometry Mailing List: RE: Thanks to all for a successful 2007 NW
Regional ... ... Purdue Cytometry Mailing List:

RE: Thanks to all for
a successful 2007 NW Regional ... Miltenyi,

Phoenix Flow Systems,
Spherotech, Tree Star, Union Biometrica, and

Verity Software House,

Ryan Brinkman, Perry Halaand, and others of the FICCS ...
http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0471.htm - 6.7KB 74%
|||||||||||||||||||| 28 Mar 07 Find Similar Highlight Purdue
Cytometry
Mailing List: Applications Specialist - iCyt - ... Northwest
Cytometry

Users Group and Cascade Cytometry Users Group are pleased to announce
the 2007 NW Regional Cytometry Meeting, which ... Tree Star, Union
Biometrica, and Verity Software House. ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0333.htm
- 7.0KB 74% |||||||||||||||||||| 30 Jan 07 Find Similar Highlight

Purdue Cytometry Mailing List: By Thread ... Purdue Cytometry Mailing
List: By Thread ... DIVA Software on MAC Intel Computers Pamela Shaw
(Wed Dec 13 2006 - 13:25:52 EST) ... http://www.cyto.purdue.edu/hmarchiv/2006/thread.htm
- 332.1KB 74% |||||||||||||||||||| 30 Jan 07 Find Similar Highlight

Purdue Cytometry Mailing List: RE: Diva 4.0 files ... Purdue
Cytometry
Mailing List: RE: Diva 4.0 files ... Mark E. Munson Sales Manager
Verity Software House, Inc. 45A Augusta Road PO Box 247 Topsham, ME
04086 Phone: 207-729-6767 x191 Fax: 207-729-5443 ...
http://www.cyto.purdue.edu/hmarchiv/2004/1443.htm - 6.2KB 74%

Document count: purdue (139318) cytometry (25468) mail (53709) list
(63448) 2007 (37553) verity (222) software (15216) sales (14402) 07
(21574) by (129519) thread (26375) purdue cytometry mail list 2007
verity software sales 07 by... (27962)
about 187244 results found, top 500 sorted by relevance
score using date   hide summaries   group by location
       spacer   1-10   next

Purdue Cytometry Mailing List: RE: DNA analysis software
... Purdue Cytometry Mailing List: RE: DNA analysis software ... J.
Herbert Technical Support Manager Verity Software House ...
http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0747.htm - 7.7KB
78%

26 May 07

Collusion is an agreement, usually secretive, which occurs between two
or more persons to deceive, mislead, or defraud others of their legal
rights, or to obtain an objective forbidden by law typically involving
fraud or gaining an unfair advantage. It can involve "wage fixing,
kickbacks, or misrepresenting the independence of the relationship
between the colluding parties."[1] All acts affected by collusion are
considered void.[2]

http://www.new.facebook.com/note.php?note_id=23879772873&ref=mf
Mitch Haynes - 27 Aug 2008 04:49 GMT
> ISAC XXII International Congress & Purdue Cytometry University ML &
> XML
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>
> - Show quoted text -
flowcytometry - 09 Dec 2008 07:31 GMT
Re: G3s G4s, MacOS and CellQuest - a cautionary tale
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From: Maciej Simm <simmmmer@yahoo.com>
Date: Wed Jan 12 2000 - 23:25:13 EST
I had a chat with someone @ BD and it sounds like MacOS X might fix those
bugs..
but no names :)

Maciej

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Received on Thu Jan 13 13:00:42 2000
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Re: G3s G4s, MacOS and CellQuest - a cautionary tale
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From: Adrian Smith <A.Smith@centenary.usyd.edu.AU>
Date: Thu Jan 13 2000 - 20:35:58 EST
>I had a chat with someone @ BD and it sounds like MacOS X might fix
>those bugs..
>but no names :)
>
>Maciej

What  I don't understand is why BD doesn't put some effort into
getting it to work properly with OS9.0 before they even think about
OS X.  If it works under OS9.0 it will work under OS X (at least that
is what has been promised), even if it doesn't take full advantage of
the new features of OS X. Besides OS X is still a year away and OS 9
has been shipping for months (and as David originally posted it is
the only choice on some machines).

Adrian
Received on Fri Jan 14 11:30:48 2000
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RE: G3s G4s, MacOS and CellQuest - a cautionary tale
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From: Newsom, Brian S. <BSNEWSOM@txccc.org>
Date: Thu Jan 13 2000 - 11:57:04 EST
David,

Sorry to hear about your woes. I wanted to pass on the information I have
regarding this issue. I have been talking to BD about upgrading some of our
FACScans to Macs with Cellquest and have talked to them briefly about G3 vs
G4. They know there are problems with OS 9.0 and they are working on them,
that is why currently they are only selling G3's. They do have OS 9.0
compatibility in the works and I would expect it would come fairly soon. In
our discussions with BD it sounded as though by the time we get our act
together to get the necessary machines they may be ready to go with G4's and
OS 9.0.

Best of luck,
Brian Newsom
Director, Flow Cytometry
Center for Cell and Gene Therapy
Baylor College of Medicine

-----Original Message-----
From: David Chambers [mailto:davidc@ccmi.salk.edu]
Sent: Tuesday, January 11, 2000 11:26 AM
To: cyto-inbox
Subject: G3s G4s, MacOS and CellQuest - a cautionary tale

Wotcher Flowers,

I thought I would share my recent experience with everyone.

Recently we had the need to set up a new CellQuest analysis workstation to
cope
with demand for Mac time.  So we ordered a new computer from Apple.  It was
a
G4 machine because that's all they sell now unless you want an iMac or
powerbook.  Of course, it shipped with MacOS 9.0 installed, and arrived last
week.  A very nice looking machine, rather reminiscent of a Porsche :-)

B-D now ships CellQuest with a USB dongle, so there were no problems with
USB-ADB adapters or the like, and CellQuest installation went fine until we
tried to run it.  Firstly the BDPAC control panel would not work because
the computer told us "BDPAC cannot  load because BDPAC cannot be found".
Secondly, CellQuest ran perfectly for 5 seconds (approximately) and then the
Finder decided to intervene, saying it wanted attention.  If we gave it
such,
it shut down CellQuest saying that the server containing it could no longer
be
found, then complaining about a Type 3 error.  Note that while the finder
could
not get our attention, CellQuest ran nicely - we could load and save files,
analyze, print, etc., but one accidental click outside the CQ window and
WHAMMO!

We tried the usual things - increasing CQ memory, turning all unnecessary
extensions off, disconnecting the network, etc, etc, all to no avail.  "No
problem", I thought, "Let's install OS 8.6, we know CellQuest is fine with
that"

Except.... THE NEW G4's WILL ONLY RUN MacOS 9.0 or LATER.  It is
totally impossible to install 8.6 on them.  We called Apple for help and
were
told that this is indeed the case and they couldn't/wouldn't help, hard
luck,
it was the Application's fault.  Apple has changed to a new motherboard in
recent machines and it is impossible to use older versions of MacOS.  So we
gave up and sent the G4 back.  We found a blue G3 machine at MacWarehouse,
it
runs 8.6 and runs CellQuest impeccably.

All the above diatribe is just to warn others thinking of getting a new
computer that they may have no prospects of being able to run CellQuest on
it.
It is sad that Apple should no longer manufacture machines capable of
running
a previous OS, particularly since OS 9.0 appears to have issues with many
other
applications software as well.

Pity.

-- David

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Re: G4's will run OS8.6
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From: Derek Davies <daviesd2@icrf.icnet.uk>
Date: Sun Jan 16 2000 - 16:21:08 EST
Hi Keith and others,

Just a few thoughts....

I don't know whether anyone else has the same 'problem', but I am still
running my Vantage under System 7.1, chiefly because it does what it is
supposed to do ie control the sort and I am unwilling to upgrade and possibly
introduce all sorts of problems (which is what happened when I upgraded a
previous working HP system to a then new Mac system) -  if it ain't broke etc.

This has caused the computer people here (God bless 'em) to tear their hair
out
and repeatedly ask me to upgrade even if only to 8.1.

I too have been wondering how BD in particular will be dealing with the
major changes that will occur with the more UNIX-like feel of OS X (OS 9 does

appear to be a sort of a stopgap). The newer G4 processors are much
speedier but we could also take the view that we are being forced into
buying hugely overspecced computers by market forces and, of course, software

follows suit - look at the bloated nature of most web browsers (with
the honourable exception of iCab) and word processing software. However,
it will be incumbent on machine manufacturers to keep up.  If I may be
Satan's pal for a moment, maybe it would be an idea to consider separate
program versions for acquisition and analysis. After all, even today's
processors are speedy enough to analyse huge numbers of cells per second
and we could keep many machines going on "outdated" hardware for years.
Then an analysis program could be freed to keep up with OS changes
without the need to make sure that it will also acquire data, speak to
the cytometer etc etc. Of course a third party program (?like FlowJo)
would do just as well and already exist.

At the moment there seems to be confusion as to which OS will run
CellQuest, whether it will work for analysis only etc. Still, could be
worse, we might be running Windows and we do have Jonathon Ive's top
designs!

Derek

On Thu, 13 Jan 2000, Keith Bahjat wrote:
<Snip fully deserved Apple eulogy>
> In the meantime, I would drop a line to BD, reminding them that OS X is
> coming in August, at which point their software will not be compatible with
[quoted text clipped - 3 lines]
> DOS (yuk!). Maybe Verity or TreeStar will make some inroads if we demand
> state of the art software.

************************************************************************
Derek Davies                       Voice: (44) 0207 269 3394
FACS Laboratory,                   FAX: (44) 0207 269 3100
Imperial Cancer Research Fund,     e_mail: derek.davies@icrf.icnet.uk
London, UK

Web Page: http://www.icnet.uk/axp/facs/davies/index.html

In tenebris lux                            
*************************************************************************
Received on Tue Jan 18 13:47:20 2000
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RE: G3s G4s, MacOS and CellQuest - a cautionary tale
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From: Carl-Magnus Hogerkorp <carl-magnus.hogerkorp@molmed.lu.se>
Date: Wed Feb 02 2000 - 09:46:40 EST
Dear All,

I wonder who to curse (BD,Verity or Apple). Having looked through all inputs
on this line of subject, I thought let's just use the WinList on the G4 in
the meantime. However, despite the use of iMate the computer can't find the
dongle and therefore won't cooperate.

OK that CellQuest doesn't work in the OS9 environment, what about Winlist is
it just the ADB connects that is the problem or is it the OS9 again?

What is possible to run on the G4?
Shall I send it back?

Carl-Magnus Högerkorp
Stem Cell Laboratory
University Hospital of Lund
Sweden

-----Original Message-----
From:    Newsom, Brian S. [mailto:BSNEWSOM@txccc.org]
Sent:    den 13 januari 2000 17:57
To:    Cytometry Mailing List
Subject:    RE: G3s G4s, MacOS and CellQuest - a cautionary tale

David,

Sorry to hear about your woes. I wanted to pass on the information I have
regarding this issue. I have been talking to BD about upgrading some of our
FACScans to Macs with Cellquest and have talked to them briefly about G3 vs
G4. They know there are problems with OS 9.0 and they are working on them,
that is why currently they are only selling G3's. They do have OS 9.0
compatibility in the works and I would expect it would come fairly soon. In
our discussions with BD it sounded as though by the time we get our act
together to get the necessary machines they may be ready to go with G4's and
OS 9.0.

Best of luck,
Brian Newsom
Director, Flow Cytometry
Center for Cell and Gene Therapy
Baylor College of Medicine

-----Original Message-----
From: David Chambers [mailto:davidc@ccmi.salk.edu]
Sent: Tuesday, January 11, 2000 11:26 AM
To: cyto-inbox
Subject: G3s G4s, MacOS and CellQuest - a cautionary tale

Wotcher Flowers,

I thought I would share my recent experience with everyone.

Recently we had the need to set up a new CellQuest analysis workstation to
cope
with demand for Mac time.  So we ordered a new computer from Apple.  It was
a
G4 machine because that's all they sell now unless you want an iMac or
powerbook.  Of course, it shipped with MacOS 9.0 installed, and arrived last
week.  A very nice looking machine, rather reminiscent of a Porsche :-)

B-D now ships CellQuest with a USB dongle, so there were no problems with
USB-ADB adapters or the like, and CellQuest installation went fine until we
tried to run it.  Firstly the BDPAC control panel would not work because
the computer told us "BDPAC cannot  load because BDPAC cannot be found".
Secondly, CellQuest ran perfectly for 5 seconds (approximately) and then the
Finder decided to intervene, saying it wanted attention.  If we gave it
such,
it shut down CellQuest saying that the server containing it could no longer
be
found, then complaining about a Type 3 error.  Note that while the finder
could
not get our attention, CellQuest ran nicely - we could load and save files,
analyze, print, etc., but one accidental click outside the CQ window and
WHAMMO!

We tried the usual things - increasing CQ memory, turning all unnecessary
extensions off, disconnecting the network, etc, etc, all to no avail.  "No
problem", I thought, "Let's install OS 8.6, we know CellQuest is fine with
that"

Except.... THE NEW G4's WILL ONLY RUN MacOS 9.0 or LATER.  It is
totally impossible to install 8.6 on them.  We called Apple for help and
were
told that this is indeed the case and they couldn't/wouldn't help, hard
luck,
it was the Application's fault.  Apple has changed to a new motherboard in
recent machines and it is impossible to use older versions of MacOS.  So we
gave up and sent the G4 back.  We found a blue G3 machine at MacWarehouse,
it
runs 8.6 and runs CellQuest impeccably.

All the above diatribe is just to warn others thinking of getting a new
computer that they may have no prospects of being able to run CellQuest on
it.
It is sad that Apple should no longer manufacture machines capable of
running
a previous OS, particularly since OS 9.0 appears to have issues with many
other
applications software as well.

Pity.

-- David

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RE: G3s G4s, MacOS and CellQuest - a cautionary tale
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From: Mario Roederer <Roederer@drmr.com>
Date: Wed Feb 02 2000 - 16:00:41 EST
Carl, don't despair--FlowJo runs fine on G4's in OS9-- you can use it
to analyze all of your data files, be they BD, Coulter, Cytomation,
or any other fine manufacturer's.

Tree Star will soon have a specific version of FlowJo that will take
advantage of the G4 "altivec" processor--allowing significant
improvements in calculation speeds by doing multiple simultaneous
calculations.  In alpha-testing, the altivec-specific version
performs compensation 2-5 times faster than the standard version!

For more information, see <http://www.treestar.com/flowjo/>

mr

At 3:46 PM +0100 2/2/00, Carl-Magnus Hogerkorp wrote:
>Dear All,
>
[quoted text clipped - 99 lines]
>
>-- David
Received on Thu Feb 3 13:57:40 2000
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Re: Paint-a-Gate
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From: Adam Treister <adam@treestar.com>
Date: Mon Feb 21 2000 - 18:41:41 EST
Helen,

I would presume that Paint-a-Gate will not read Summit's files
because BD's software expects 8 or10 bits per data value, and
Summit writes 12 bits.

FlowJo has a hidden feature in the Export function that would let
you write the files in a format Paint-a-Gate could read (I think).
This is not visible in the "consumer" version of the program but I'd
can issue you a special serial number to do it.  As Larry pointed
out, Summit can create these graphs, (as can FlowJo) so doing this
translation step may not be worth the trouble.

For more on FlowJo's color gating and overlays, see:
<http://www.treestar.com/flowjo/v3/html/leoverlays.html>

Adam

>  At 02:31 PM 2/16/00 -0600, Helen Horton wrote:
>A very quick question: Does anyone know if data acquired by Summit
>software (Cytomation) can be analyzed by Paint-a-Gate software (BD)?
>Neither company could answer this for me!

----->>> On 21-Feb-2000, Larry Arnold wrote:
>   If you have acquired data with Summit why not use Summit's
>   color gating feature which is equivalent to Paint-a-Gate?
--------------

----------------------------------
Adam Treister
Tree Star, Inc.
ph:  650-508-9349
fax:  650-508-9186
www.treestar.com/flowjo
----------------------------------
Received on Tue Feb 22 12:14:04 2000
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Re: OS9+Cellquest
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From: Adrian Smith <A.Smith@centenary.usyd.edu.AU>
Date: Wed Mar 08 2000 - 19:40:50 EST
>Cell Quest runs on G4s running OS9.  You just need to purchase a USB to ADB
>adapter to attach your dongle.  I recommend the I-Mate from Griffin
>Technologies.  Then download the recent version of the driver from Griffin's
>website (http://www.griffintechnology.com/imac/imate_driver.html), which
>will support the dongle.

Well it doesn't work in the lab next door with a USB dongle and it
certainly didn't work for David Chambers (see his post to this list
on 13.1.00).

Next door haven't tried it with a USB-ADB adaptor which makes me
wonder if the USB dongle might be part of the problem with Mac OS 9.0

We are still using it on a PowerMac 7300/180 for very occasional
work. Any heavy duty analysis is done in FlowJo.

Adrian

--
******************************************************
Adrian Smith (PhD Student)        T CELL BIOLOGY GROUP
Centenary Institute of Cancer Medicine & Cell Biology
Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA.
******************************************************
Received on Thu Mar 9 10:25:20 2000
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FlowJo and CellQuest (was RE: OS9+Cellquest)
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From: Adrian Smith <A.Smith@centenary.usyd.edu.AU>
Date: Wed Mar 08 2000 - 20:33:48 EST
>OK Flow JO is great ANALYSIS software, for most things....but it still will
>not Acquire data,

I'm not really sure that is the point here. Haven't we been talking
about ANALYSIS?

Most, if not all, the people who have been having problems have
bought new G4 machines for post-acqusition analysis (and other uses).
Those who simply upgraded to OS 9.0 could easily downgrade to OS8.6
when it didn't work - it was those people with machines that only run
OS9.0 that were stuffed (by Apple and by BD)

If you bought one for acquisition then I agree wholeheartedly that
you should consider it an object lesson (esp given *BDs* record (AND
their recommendations) as well as Apple's)

>   allow back gating of populations

Excuse me?

>and is not the easiest
>for "Graphical" Analysis...

That is entirely subjective (and I'm not even sure what you mean by
"graphical" analysis!). As I have said before I find that complete
novices pick up FlowJo MUCH more quickly than CellQuest.

>so the mantra of "dumping" Cellquest and moving
>to FlowJo is over simplistic at best and is only a solution for those who
>never touch the FACS their data comes from in the first place.

Not true either. I touch the flow cytometer while it collects all my
data AND I always use FlowJo for my analysis. No, FlowJo is not a
solution for acquisiton but again I don't think that is what we have
been talking about.  FlowJo has been an excellent solution for us and
we do all our own acqusition (using CellQuest on an older machine
with an older OS - as you said, if it ain't broke...).

Our data is of such a nature that ANY increase in machine speed
directly translates to increased analysis throughput - FlowJo has
allowed us to take advantage of those increases in speed. Analysis
takes MUCH longer the acqusition in our experiments  and acqusition
doesn't require any where near the same computing power.

We were even using FlowJo while the cytometer was still running from
an HP - CellQuest nowhere in the loop!

I think acquisition and analysis are two separate tasks and it makes
sense to use the best tools for each one.

Most people need two computers, and hence two software licenses, anyway...

I don't think I was being "simplistic" in advocating dropping CellQuest :)
( but I should have made it clearer I was talking about ANALYSIS only)

>And it still
>isn't available for the PC platform, so once again, it is not a cure-all.

Neither is CellQuest last time I looked!
Received on Thu Mar 9 11:25:22 2000
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G4 and OS8.6
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From: <David.McFarland@mcmail.vanderbilt.edu>
Date: Wed Mar 08 2000 - 10:38:13 EST
OK. Imade the mistake of passing along some (mis)information from our
computer
"expert".  I have received numerous responses from this forum that say it is
indeed possible to install an OS pre-OS9 on a G4.  I'd say contact Apple to
get
the real lowdown.  Sorry if I lead anyone astray.

  David McFarland
  Howard Hughes Medical Institute
  Flow Cytometry Facility
  Vanderbilt University Medical Center
Received on Wed Mar 8 18:10:16 2000
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CellQuest and OS9 and new G4s - Resolved!
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From: David Chambers <davidc@ccmi.salk.edu>
Date: Thu Mar 16 2000 - 14:47:08 EST
I thought I would just report to the list that BD has done a really great job
on making the above combination work correctly.  Kudos to them.  We received
the new version of CellQuest this morning and it works just perfectly!

-- David
Received on Wed Mar 22 11:11:40 2000
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Re: Applescript and Flowjo?
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]
From: Adam Treister <adam@treestar.com>
Date: Thu Apr 06 2000 - 19:19:50 EST
----->>> On  6-Apr-2000, Jan Hendrikx wrote:
>   Has anybody succeeded in scripting Flowjo using Applescript?
>   It would be great if that were possible!!
--------------
I know I haven't.

As Adrian noted, FlowJo does support some minimal level of AppleScript, but
doesn't have the object model implementation which is what you'd need to do
anything meaningful.  I did this in an earlier application (FlexiTrace) and
it turned into a huge amount of work to enable obtuse functionality to a
small number of users.  My personal experience with AppleScripts is that
they're really brittle.  If you move something, or want to something
different from the script writers intention, the script gets annoyingly
confused and its much easier to start over than to fix it.   Publish and
Subscribe is an easier addition feature we've considered, but there just
aren't many people who'd take advantage of this if it were included.  (Let
me know if you think otherwise.)

If properly constructed, a workspace in FlowJo is the script of all
analyses and reports in that experiment.  Dropping your new data folder
onto the workspace should perform all the analyses that one might automate
with AppleScript.  We have considered automating it further, but the
logical next step is auto-printing.  In deference to trees, we chose to
stop short of automating the output without making you look at the data.

Someday soon, FlowJo will store its workspace in an XML syntax, which means
that we can utililze generic scripting tools, or you can even hack the
files yourself.  I think this is a better approach than addiing
AppleScript.

Adam

----------------------------------
Adam Treister
Tree Star, Inc.
ph:  650-508-9349
fax:  650-508-9186
www.treestar.com
----------------------------------
Received on Fri Apr 7 12:51:48 2000
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FlowJo/G4/OS X update [was: Cellquest/G4/OS9 update]
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From: Adam <adam@treestar.com>
Date: Thu Apr 27 2000 - 23:53:34 EST
bunny wrote:

> And Adam- as soon as FlowJo does histogram overlays (the only thing I
> use CQ for these days)- I'm there!

FlowJo does histogram overlays.  See
<http://www.treestar.com/flowjo/v3/html/leoverlays.html>

I called Bunny, and she was referring to the Kolmogorov-Smirnoff
statisitic to perform histogram comparison.  FlowJo will create and
export the Cumulative Distribution Functions (the integral of the
histogram) which is the basis of the K/S statistic, so you can easily
perform this test with the help of a spreadsheet.  But we still don't
have that function built in.  I have a prototype built, and you'll
likely see this feature included one day soon.

We'll probably get it into the OS X compatible release, due when Apple
releases the new operating system in the summer. But the tools we need
don't get released until next month, so its hard to predict the
schedule.  Nevertheless, FlowJo worked with OS9 and the G4 the day they
shipped, and we'll endeavor to support OS X on that same timeframe.

> BTW- CellQuest FLIES on a G4!

Of course, that's relative to CellQuest on a G3.  On the same equipment
with the same data, FlowJo outperforms CellQuest hands down.

Adam

-----------------------------------
Adam Treister
Tree Star, Inc.
www.flowjo.com
800-366-6045
-----------------------------------
Received on Fri Apr 28 14:17:53 2000
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Interest in a FlowJo Users Meeting at ISAC?
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From: Adam Treister <adam@treestar.com>
Date: Wed May 10 2000 - 18:44:43 EST
Dear ISAC Attendees:

I've received a few requests to hold a FlowJo Users Group meeting at the
ISAC conference.  If this is something you'd be interested in, please drop
me a note.  If I get sufficient response then I'll arrange a time and place
to meet.  Of course, you're also welcome to bring your questions (and bring
some data!) to our booth (#105) for one-on-one assistance.

Tree Star is sponsoring an "Internet Lounge" at the conference, providing
access to email and the web so that you can keep in touch.  We will provide
several computers, as well as hookups for your laptops.  If you have
special software or computing needs, please let me know and we'll try to
accommodate you.

I look forward to seeing you all in Montpellier,

Adam

----------------------------------
Adam Treister
Tree Star, Inc.
ph:  650-508-9349
fax:  650-508-9186
www.treestar.com
----------------------------------
Received on Thu May 11 11:30:51 2000
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G4 for FACSCalibur
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From: Jeannine Navratil <jsn9@imap.pitt.edu>
Date: Tue Sep 19 2000 - 16:14:01 EST
Hello all!

We want to upgrade our FACSCalibur computer from a Power PC to a G4.  I
wanted to play it safe and purchase it through BD, but the powers that
be think that their price is ridiculously inflated, when we could buy a
G4 ourselves for considerably less money.  Has anyone done this, and if
so, did you run into problems?

My concerns are 1.  Installing the card that connects to the cytometer,
2.  Making sure that the operating system is compatible with the
cytometer and CellQuest software,  3.  What does this do to our service
contract coverage, since now the computer is no longer covered?

Any advice would be much appreciated!

Jeannine Navratil
University of Pittsburgh
Arthritis Institute
Received on Wed Sep 20 11:28:27 2000
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G4 for FACSCalibur
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From: Jeannine Navratil <jsn9@imap.pitt.edu>
Date: Wed Sep 20 2000 - 17:55:54 EST
Hello all again!

Well, I've learned a bit since I posted the message yesterday.  I spoke
with technical support at BD, and according to them, the electronics
card that is in our Power PC (7600/120) is compatible with a G4:  it
just needs to be moved to the PCI slot in the new computer.  As far as
compatibility, any G4 that does NOT have a dual processor has been
tested and is compatible with CellQuest 3.3.  Since the dual processor
G4s are the latest hot thing, the old G4s are now being sold for bargain
prices at places like MacMall or MacConnection.

As far as the service contract goes, buying and connecting up our own
computer does not affect our instrument support, but the new computer is
not covered under that contract.

The bottom line is, you can do this yourself for considerably less than
what BD would like you to pay.  I'll keep everyone posted as to how we
make out with this.

                                                       Sincerely,

                                                       Jeannine
Navratil
                                                       University of
Pittsburgh
Received on Thu Sep 21 13:58:34 2000
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Re: G4 for FACSCalibur
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From: Matthias Haury <mhaury@igc.gulbenkian.pt>
Date: Thu Sep 21 2000 - 05:12:47 EST
Hey y'all,

I have upgraded our Calibur to a G4, it's easy if you already have a PCI
BDIO Board (from 7600 PPC or G3), if you have the Quadra, you will need to
purchase another PCI board from BDIS, which I guess is difficult to buy
separately...

Also if you do so, you should buy an i-Mate USB-ADB converter to connect the
ADB Dongle to the USB Bus (or have a new USB dongle from BDIS), also make
sure that you are not using any SCSI Devices for backup, or order a separate
SCSI 2906 Card from Adaptec for the G4.

You also need System 9.04, and BD Inits 3.4 and Cellquest 3.3 (all of which
I guess you can get from BDIS as free upgrade ???)

Last not least you have to know that BDIS of course will no longer cover any
problems related to the computer or BDIO communication, so if you have a
service contract you might loose the protection for all I/O related problems
(this you have to discuss with your BDIS rep).

We have no problems because I don't have a service contract, and we have
enough competence for all Mac OS related problems...

Besides this it works beautifully...

Hope that clarifies things..

Matthias

_____________________________________
Matthias Haury
Instituto Gulbenkian de Ciencia
Rua Da Quinta Grande N° 6 - Apt.14
P-2781-901 Oeiras Codex
Portugal
Email: mhaury@igc.gulbenkian.pt
Tel: + 351 21 440 79 85 Office
Fax: + 351 21 440 79 70
_____________________________________

> From: Jeannine Navratil <jsn9@imap.pitt.edu>
> Organization: University of Pittsburgh
[quoted text clipped - 20 lines]
> University of Pittsburgh
> Arthritis Institute

Received on Thu Sep 21 15:43:34 2000
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FlowJo Training in Boston Sept 26
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From: Adam Treister <adam@treestar.com>
Date: Wed Sep 20 2000 - 14:02:17 EST
It is gratifying to see a growing demand we're getting for training in how
to use FlowJo.   There are more and more people hearing about the program,
realizing that it can help them in their analysis, and wanting to learn its
novel approach to experiment based analysis.

I will be giving two training sessions in using FlowJo software in the
Longwood area of Boston on Tuesday, Sept 26.  There is a morning session
from 11:30 am - 1:00pm at Dana Farber Cancer Institute, in Dana 5
Conference Room (Rm D521) and an afternoon session from 2:00 to 3:30 in the
Level 6 Conference Room at the Harvard Institute of Medicine Building.

Both sessions are open to the general flow community.

------------------------------------------------------------
Adam Treister      Tree Star, Inc.
ph: 1-650-508-9349  fax: 1-650-508-9186
adam@treestar.com   <http://www.treestar.com>
------------------------------------------------------------
Received on Thu Sep 21 11:43:34 2000
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Re: G4 for FACSCalibur
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From: Ray Lannigan <lannigan@tritechinc.com>
Date: Thu Sep 21 2000 - 08:54:56 EST
Jeannine,
   The cytometer interface card that is inside your Power PC can be put
into a G4. The installation is simple enough to be done by yourself or your
in house computer people. If neither of those options are feasible, give me
a call I can help you do it. It is my understanding that when upgrading to
OS9 or greater from OS8.6 or lower, there is an incompatibility issue with
the BD inits.There was a e-mail posted by a BD rep.on March 8, 2000 that
addresses the incompatibility issue. You can refer to this e-mail through
the archives, or I can forward you a copy if you prefer. As far as your
service contract; Tritech can provide you with a service contract for your
FacsCaliber with the new G4 for a price that is considerably lower than
BD's. If you would like more information please contact me at one of the
numbers listed below.
Regards,
Raymond Lannigan
Tritech Inc.
2014 Renard Ct. Suite I
Annapolis, MD 21401
1-410-266-1522
1-800-886-7004
www.tritechinc.com
-----Original Message-----
From: Jeannine Navratil <jsn9@imap.pitt.edu>
To: cyto-inbox
Date: Wednesday, September 20, 2000 5:53 PM
Subject: G4 for FACSCalibur

>Hello all!
>
[quoted text clipped - 14 lines]
>University of Pittsburgh
>Arthritis Institute

Received on Thu Sep 21 14:28:38 2000
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Re: G4 for FACSCalibur
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From: D. Robert Sutherland <rob.sutherland@utoronto.ca>
Date: Fri Sep 22 2000 - 12:55:50 EST
There are several cheaper ways to upgrade non-G3, Power PC-based CPUs on
your FACStation, without buying a new G4 or dual processor G4.

As pointed out by other correspondents, if you buy a G4, you will
probably have to deal with a variety of 'connectivity issues' such as
the need for USB connectable printers/adaptors, dongles and any other
SCSI-based devices currently in use such as Zip drives etc.  Although
these issues can be accommodated (at a cost) as Matthias et al have
suggested, dealing with such problems has caused major headaches for a
number of users tempted to upgrade to G4s.  Furthermore, to my knowledge
at least, BD have not upgraded Cellquest to take advantage of the
Altivec processing capability of the G4 chip.  Therefore, Cellquest will
not run significantly faster on a G4, than on a G3 rated at the same
processor clock speed.  Similarly, Cellquest will not run any faster on
a dual processor G4 running OS 9.x.  While the upcoming Mac OSX will
allow many more applications to take advantage of the dual G4s, a major
re-work of Cellquest will most likely be required for OSX
compatibility.  Are BD even planning such an upgrade, and if so, when?

Instead, for about US$200.00, you can purchase a G3 upgrade card
(running at about 400Mz with a 1meg backside L2 cache) for your
7300/7500/7600 Power PC Mac and make a huge gain in performance.  Such
cards are incredibly easy to install by swapping out the old PPC604e
card found in all PCI-based Macs.  Even faster upgrades are available
and you can even get G4 upgrade cards for these Macs if you really want
one.  However, unless you are planning to use your FACStation computer
to run Altivec enhanced applications such as the recent version of Adobe
Photoshop et al, I see little reason to go through all the connectivity
hassles (and cost) for little if any gain.

This may not be everyone's idea of fun, but I recently purchased as
separate items, a 500Mz ZIF (the 'processor') and a 'PCI Carrier card'.
(You can also get them as a single card with the ZIF already 'soldered'
into the card).  I removed the 266 ZIF from the 'Beige' G3 in my office
and replaced it with the 500Mz ZIF.  I then installed the 266Mz ZIF into
the carrier card and installed it into a 7300 running our dear old
FACScan.  The 200Mz PPC 604e card that I removed from the 7300 was then
installed in a Calibur running off a 7600 containing an even older 120Mz
PPC 604.  Thus, we managed to upgrade 3 Macs for the price of one, we
have not had to deal with connectivity issues with any of them,  and all
we have left over is one old PPC 604 card to use as a door stop.

My 2c worth.

Rob Sutherland
University Health Network
University of Toronto
Received on Mon Sep 25 13:58:57 2000
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FlowJo v3.2 adds new Histogram Comparisons
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From: Adam Treister <adam@treestar.com>
Date: Sun Jul 23 2000 - 01:15:45 EST
Dear Flow-ers,

We have released a new version of FlowJo, available for immediate download.
Along with sundry bug fixes and refinements, this version contains a
powerful new algorithm for comparing histograms.

Version 3.2 of this popular off-line analysis program provides a unique
integrated platform for comparing univariate distributions.  FlowJo
provides the standard K-S statistic, with its inherent limitations, but we
also provide a new metric to compare histograms.  This "Chi-Squared T
Value"  provides an indication of the statistical significance with which
two histograms are different, and its value scales with the degree of
difference.  This means that, for the first time, histograms can be ranked
by a value that conveys how different they are!

We would like to invite everyone to explore the power of this new
algorithm.  For more information, see
<http://www.flowjo.com/v3/html/comparison.html>.

We are maintaining our policy that allows you to download and try FlowJo
for 60 days at no charge.  See <http://www.flowjo.com> for more information
and download instructions.

Adam

----------------------------------
Tree Star, Inc.
New Phone Number:  1-650-591-2854
Or Toll Free (in US): 1-800-366-6045
fax:  1-650-508-9186
www.flowjo.com
----------------------------------
Received on Mon Jul 24 11:41:31 2000
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Re: Applescript and Flowjo?
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From: Adrian Smith <A.Smith@centenary.usyd.edu.AU>
Date: Thu Apr 06 2000 - 18:04:46 EST
>    Dear Flowers,
>
[quoted text clipped - 5 lines]
>Jan
>--

There appears to be a small AppleScript dictionary for FlowJo 3.1 but
it looks to be just the standard commands, ie nothing specific to
FlowJo. Not knowing anything about programming in Apple Script I have
no idea if this is of any use but you might be able to do something
with them...

What sort of things did you want to do with AppleScript? Personally I
can't really thing anything that I do that AppleScript would make
easier/quicker, but that may be just because I don't know much about
AppleScript.

Depending on what you want to do you might be able to do it with one
of the macro programs like OneClick, Quickeys or Key Quencer. A while
ago I made a simple OneClick palette to make some FlowJo features
more accessible. It saved me a bit of time for a while but I
eventually gave up using it because OneClick seemed to decrease
FlowJo's stability.

Adrian
Received on Fri Apr 7 12:06:47 2000
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Re: Apple grumble...
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From: Adrian Smith <A.Smith@centenary.usyd.edu.AU>
Date: Mon Aug 07 2000 - 15:32:51 EST
At 4:41 PM -0700 4/8/2000, David Chambers wrote:
>  >
>>       Okay, is there any way that I can pursuade Cell Quest version 3.1 to
[quoted text clipped - 7 lines]
>
>-- David

I'm pretty sure we have it running for analysis only on our G4/400
with OS9.0.4. We have a Griffin iMate for the dongle (USB to ADB
convertor) and I think we just copied it from our old G3 (along with
the EVE INIT for the dongle).

I'll confirm when I get into the lab...(I don't actually use it myself)

What exactly have you tried David?

Adrian Smith
Received on Tue Aug 8 12:43:32 2000
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Re: Apple grumble...
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From: Adrian Smith <A.Smith@centenary.usyd.edu.AU>
Date: Tue Aug 08 2000 - 20:11:22 EST
At 12:48 PM -0700 8/8/2000, David Chambers wrote:
>  >
>>  What exactly have you tried David?
[quoted text clipped - 8 lines]
>
>--D

Just to confirm that we have no problems doing analysis with
CellQuest 3.1f on a G4/400 (AGP graphics) with MacOS 9.0.4. No
problems with the Finder at all...

We use a iMate for Griffen to plug the ADB dongle into the the USB port.

We just copied the CellQuest application folder and the EVE INIT
(version 1.10) over from our old G3 and every has worked perfectly.

The only problem is that once CellQuest has been run, FlowJo will not
recognise its ADB dongle until we restart.

I suspect the 9.0.4 might be an important factor here (the update was
released the day we received the machines so we have never tried
things with an earlier version of OS9).

Adrian Smith
Received on Wed Aug 9 12:43:38 2000
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Re: G4 for FACSCalibur
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From: Keith Bahjat <kbahjat@ufl.edu>
Date: Mon Sep 25 2000 - 18:24:38 EST
> Furthermore, to my knowledge
> at least, BD have not upgraded Cellquest to take advantage of the
[quoted text clipped - 6 lines]
> University Health Network
> University of Toronto

Actually, BD never upgraded CellQuest to take advantage of the PowerPC (i.e.
modified to be a native PowerPC app), much less AltiVec!! I wouldn't hold my
breath for the AltiVec enhanced cellquest any time soon...

BUT, if you use other applications on your cytometer's computer, the G4 may
not be a bad idea. Apps like Photoshop utilize AltiVec and run incredibly
quick on the G4. Nice to have for the future...

The iMate (USB to ADB adapter) is cheap and easy to install, and in my
experience gives 0 trouble. I don't know how much of a "hassle" it is to
connect an extra adapter to the end of the Cellquest dongle?? Certainly less
of a hassle than installing and configuring a G3 upgrade card..

Keith Bahjat
kbahjat@ufl.edu
Received on Tue Sep 26 13:59:03 2000
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Re: G4 for FACSCalibur
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From: Mario Roederer <Roederer@drmr.com>
Date: Tue Sep 26 2000 - 15:16:30 EST
I tested FlowJo for a number of different tasks (e.g.,generating
complex graphical layouts; generating large tabular outputs; even
analyzing 13-parameter, million-event data files) on G4's vs. G3.
The G4 was significantly faster (taking into account the clock
speed), even when the program was not G4-optimized.  I also tested a
G4-optimized version of FlowJo that takes advantage of the AltiVec
instructions (no, not yet commercially available!), and it's another
large step up.

As more and more applications come out to be AltiVec-aware (and they
are!), everyone will realize the enormous speed benefit from this
technology.

Note that the multi-processing nature of Altivec should not be
confused with the multi-processing capability of dual-processor
machines (like the new G4 cube).  There's a completely different set
of optimizations that programs need to fully take advantage of
multi-processor machines (and I'm sure that will be forthcoming in
the next year or two as well).

Bottom line--G4's are faster, but G3's are a lot cheaper (right now).
But as more and more applications are Altivec-aware, then there won't
be a comparison.

mr

(PS--Intel-based machines, even the gigaherz processors, don't come
close to the G4 capabilities.)
Received on Wed Sep 27 12:44:13 2000
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Re: G4 for FACSCalibur - and a dig at Mario!!
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From: J. Paul Robinson <jpr@flowcyt.cyto.purdue.edu>
Date: Wed Sep 27 2000 - 20:23:51 EST
So Mario, when are you going to talk about software that runs on computers
that
the other 90% plus that people have.......in terms of world computer
sales, Apple is right up there at .....4%!!
Paul

Date sent:        Tue, 26 Sep 2000 16:16:30 -0400
To: cyto-inbox
From:            Mario Roederer <Roederer@drmr.com>
Subject:        Re: G4 for FACSCalibur

> I tested FlowJo for a number of different tasks (e.g.,generating
> complex graphical layouts; generating large tabular outputs; even
[quoted text clipped - 24 lines]
> (PS--Intel-based machines, even the gigaherz processors, don't come
> close to the G4 capabilities.)

J.Paul Robinson, Ph.D.,
Professor of Immunopharmacology
Professor of Biomedical Engineering
Director, Purdue University Cytometry Laboratories
Hansen Hall, Roon B050
Purdue University, West Lafayette, IN 47907-1515
(Ph) (765) 494-0757  Fax (765) 494-0517
jpr@flowcyt.cyto.purdue.edu

http://www.cyto.purdue.edu

http://www.bioscope.org
Received on Thu Sep 28 13:55:27 2000
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RE: G4 for FACSCalibur - and a plug for Mario!!
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From: Fischer, Randy (NIAMS) <fischer1@mail.nih.gov>
Date: Thu Sep 28 2000 - 16:59:18 EST
Paul,

Like you and many others in this world, I have one of the other 90% of the
computers at home.  I do not like it, but the other major occupant of the
household (my wife) required it for her job.  However, like 90% of the Folw
Cytometry users out there, I have a BD instrument that uses a MAC to acquire
and analyze my data.  So, I subscribe to the "do work at work" theory and
use the MAC, which by the way I do prefer.  Mario's (and Adam's) FlowJo
software is a very nice alternative, and any software that is an alternative
to paying big bucks to certain Flow companies should be most heartily
encouraged.

So, let Mario plug away and let the users of the minority instruments tout
their advantages if they wish as well.  That is, if there are any???

Randy Fischer
NIAMS/NIH

> ----------
> From:        J. Paul Robinson
[quoted text clipped - 56 lines]
>
> http://www.bioscope.org

Received on Fri Sep 29 14:39:45 2000
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RE: G4 for FACSCalibur - and a plug for Mario!!
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From: Adrian Smith <A.Smith@centenary.usyd.edu.AU>
Date: Fri Sep 29 2000 - 19:06:31 EST
And even if you are using the "other" platform for acqusition, you
can always buy a Mac just to run FlowJo. Some of us think it is that
good...

Adrian

>Paul,
>
[quoted text clipped - 28 lines]
>
>  >
Received on Tue Oct 3 13:49:44 2000
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RE: G4 for FACSCalibur - and a plug for Mario!!
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From: Len Brown <lbrown@mmri.mater.org.au>
Date: Sun Oct 01 2000 - 21:03:33 EST
Dear Randy

I have no preference when it comes to computers, except like the >90% of
computer literates, I was "brought up" on IBMs, DOS and Windows. The Macs
ARE extremely powerful (and rather expensive) but I do find it easier to
operate a Windows based PC because I am more familiar with them. I now work
with BD cytometers but initially worked on Coulters with their "so-called"
redundant operating System II software, which I thought was great for the
jobs I was doing.

To my point, like many Institutions, we have an in-house computer network
which runs on Windows NT. We have invested heavily in PCs and it makes sense
to use the network to analyse flow data. The alternative is to purchase
additional Macintosh computers, not a cheap option. I transfer data from the
MACS to our NT server where all members of the Institute can access their
data using Windows software. We currently use FCS Express and users are
generally very happy with it.

FlowJo is a great package and if you need the facilities it offers such as
software compensation and ratios etc. it is worth having a copy on your
Macs. Also, if you need the enhanced processing power of the new G4s then
that's the way to go. Otherwise, I don't see there is a great argument
either way.

Use the tools that suit the job!

Len Brown
Mater Medical Research Institute
Brisbane, Australia

-----Original Message-----
From: Fischer, Randy (NIAMS) [mailto:fischer1@mail.nih.gov]
Sent: Friday, September 29, 2000 7:59 AM
To: cyto-inbox
Subject: RE: G4 for FACSCalibur - and a plug for Mario!!

Paul,

Like you and many others in this world, I have one of the other 90% of the
computers at home.  I do not like it, but the other major occupant of the
household (my wife) required it for her job.  However, like 90% of the Folw
Cytometry users out there, I have a BD instrument that uses a MAC to acquire
and analyze my data.  So, I subscribe to the "do work at work" theory and
use the MAC, which by the way I do prefer.  Mario's (and Adam's) FlowJo
software is a very nice alternative, and any software that is an alternative
to paying big bucks to certain Flow companies should be most heartily
encouraged.

So, let Mario plug away and let the users of the minority instruments tout
their advantages if they wish as well.  That is, if there are any???

Randy Fischer
NIAMS/NIH

> ----------
> From:        J. Paul Robinson
[quoted text clipped - 56 lines]
>
> http://www.bioscope.org

Received on Tue Oct 3 14:22:17 2000
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Re: G4 for FACSCalibur
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From: Karim Vermaelen <Karim.Vermaelen@rug.ac.be>
Date: Mon Oct 02 2000 - 07:41:27 EST
"J. Paul Robinson" wrote:

> So Mario, when are you going to talk about software that runs on computers that
> the other 90% plus that people have.......in terms of world computer
> sales, Apple is right up there at .....4%!!
> Paul

So if FlowJo was a PC-only application I would have to buy a Wintel machine
on top
of my Mac, but that wouldn't matter because we're a minority anyway?
The 4% your talking about are indeed an estimate of worldwide Apple hardware
sales, but we're talking education/academic sector here, where the Mac market
share rises to 20% (and much higher in the flow cytometry area as we all know)
. I'
ve worked on both platforms and believe me I wouldn't even think about doing
my
FACS work (or any other daily lab tasks) on an NT machine. This may not be
very
obvious for someone with an engineering background, but I just like the fact
that
I can easily do maintenance and troubleshooting on our Mac network without
any
expert IT support.
So all things taken into consideration I think the choice of developing
software
such as FlowJo on Macs is a very pragmatic one.

But the real shocking news now is this report by Keith Bahjat stating that
Cellquest is not only non-optimized for the G4's vector processing, but was
never
optimized for the PowerPC architecture as well! What a shame and a waste of
power:
a 68k-application on a G4!! I hope someone from BD is reading this and can
comment
on this shortcoming in development.

Karim Vermaelen
Pulmonary Immunobiology
Ghent University Hospital
Ghent, Belgium

> Date sent:              Tue, 26 Sep 2000 16:16:30 -0400
> To: cyto-inbox
[quoted text clipped - 42 lines]
>
> http://www.bioscope.org
Received on Tue Oct 3 09:52:54 2000
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Re: G4 for FACSCalibur
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From: David Chambers <davidc@ccmi.salk.edu>
Date: Tue Oct 03 2000 - 11:54:05 EST
On Mon, 02 Oct 2000, Karim Vermaelen wrote:

> But the real shocking news now is this report by Keith Bahjat stating that
> Cellquest is not only non-optimized for the G4's vector processing, but was never
> optimized for the PowerPC architecture as well!

I don't speak for B-D, but on the CellQuest box is a large red starry sticker
saying "Accelerated for Power Macintosh".   Presumably this is not there by
accident? Naahhhh... :-)

-- David
Received on Tue Oct 3 18:49:46 2000
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Re[2]: G4 for FACSCalibur
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From: James Weaver 301-594-5879 FAX 301-594-3037 <WEAVER@CDER.FDA.GOV>
Date: Tue Oct 03 2000 - 14:49:02 EST
However since BD is currently developing and testing software on an NT
platform for their sorters, this entire discussion will become even more
pointless than it is now within the next year or two.

-Jim Weaver
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RE: G4 for FACSCalibur - and a plug for Mario!! and one more comment..
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From: J.Paul Robinson <jpr@flowcyt.cyto.purdue.edu>
Date: Wed Oct 04 2000 - 12:27:16 EST
The issue of platforms actually does become important and more
so than if you just like one more than the other. I don't want to
bash this issue, but last week the New York Times reported Apple
stock fell 58% and things are not so rosy it seems, and today in
the Wall Street Journal Apple looks like its going to take another
hit..with lost sales and production goals... worlwide sales dropped
to 3.2% .

The issue really is important - Coulter once invested heavily in the
Terak system becuase it had fantastic graphics (and it did..) it was
very expensive and many people invested .. (like us) but I suspect
there is not a single one operating today.

B-D appears to be walking a fine line - they went from HP I think  to
MACs and from what I could gather at the ISAC meeting in May
they appear to be pushing a hybrid PC data collection system and
a MAC analysis system. It is unclear to me if they are going to PC
or not. I suspect that you don't just jump from one platform to the
other --- anyway, this is an issue of sorts since in looking to
purchase a new system for our lab, I need to appreciate the
complicating factors of how to deal with a lab that has 40 odd PCs
and a MAC......and the real question for me is not how good the
MAC is, but how long it will be around....is the cube another
NEXT...???

I like FloJo by the way - think its great software !!!

Paul Robinson
On 30 Sep 2000, at 11:06, Adrian Smith wrote:

And even if you are using the "other" platform for acqusition, you
can always buy a Mac just to run FlowJo. Some of us think it is that
good...

Adrian

>Paul,
>
[quoted text clipped - 28 lines]
>
>  >
J.Paul Robinson, PhD        PH:(765)4940757
Professor of Immunopharmacology
Professor of Biomedical Engineering
Purdue University      FAX:(765)4940517
EMAIL:jpr@flowcyt.cyto.purdue.edu
WEB: http://www.cyto.purdue.edu
Received on Fri Oct 6 13:36:23 2000

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Re: BD and windows based platform
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From: Marilyn Dietrich <mdietrich@mail.vetmed.lsu.edu>
Date: Wed Oct 04 2000 - 13:31:39 EST
I hope that BD is developing and testing software on a windows 2000
platform since it is replacing NT.  If everything works fine with NT it
more than likely will work with windows 2000, however, just in case there
are problems I still hope they've move on to 2000.

-Marilyn Dietrich

At 03:49 PM 10/3/00 -0400, you wrote:

>However since BD is currently developing and testing software on an NT
>platform for their sorters, this entire discussion will become even more
>pointless than it is now within the next year or two.
>
>-Jim Weaver

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BD and Coulter
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From: Jeffrey M Scott <jeffreys@bcm.tmc.edu>
Date: Wed Oct 04 2000 - 16:31:19 EST
      Randy Fischer wrote "However like 90% of the
Flow Cytometry users out there, I have a BD instument" ?!?
      I was under the impression that the distibution
      of brands was close to 50/50. Perhaps it varies
by geographical region

                                      JMScott
                                      Core Facility
                                      Baylor College of Medicine
Received on Fri Oct 6 10:50:07 2000
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re: RE: G4 for FACSCalibur - and a plug for Mario!! and one more comment..
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From: D. Robert Sutherland <rob.sutherland@utoronto.ca>
Date: Tue Oct 10 2000 - 01:31:31 EST
My, My,
I certainly stirred up a hornets nest with my last post, the simple
objective of which was to point out how technically undemanding and
cheap it is to upgrade 'legacy' Macs such that even those in the
7300/7500/7600 family can be made to go as fast as 'you know what off
the proverbial shovel'.  And all without any help from or taxes paid (in
the form of 'all-new G4 workstations') to BD!!

Since then, we have heard disturbing news from Keith who reckons that BD
have not even upgraded Cellquest to PowerPC native code, let alone to be
Altivec enhanced (for G4s, or upgraded G4s).  Keith also politely (I
think) pointed out that is very simple to install a ADB:USB dongle
(which it now is).  However, I recall when the drivers first came out
for this adaptor, a number of users had problems.  Of course they have
been updated (yet another new one last week) to the point where even a
PC user could probably do this without calling the IT department.
The point however is that other connectibility issues outlined in my
earlier epistle (such as installing SCSI cards, USB Zip drivers etc.
serial/AppleTalk printers, floppy drives, Superdrives, etc.) are nowhere
near as simple, cheap or idiot-proof as installing a G3 or G4 upgrade
card into a fully functional workstation, that in many labs is networked
to other cytometers, computers, printers etc (i.e., legacy peripherals).

Next, Mario opined that the G4 was 'significantly faster' even taking
into account the clock speed of the processor'.  The only explanation
for this observation is that other parts of the G4 are responsible for
these speed increases.  G4s come with more (and faster) RAM, more (and
faster) Video Ram, the Bus speed is faster, the Video subsystems are
faster (blah blah).
However, for non-Altivec enhanced apps, G4s are NOT any faster than G3s
at the same clock speed, as orginally stated.  There have been hundreds
of controlled tests performed (check out xlr8yourmac.com) comparing the
same computer with a G3 or a G4 (at the same clock speed) on Altivec
enhanced versus non-enhanced apps: The results are always the same, the
G4 blows the doors off G3s (and PCs) on Altivec enhanced apps such as
Photoshop.  On other apps there is NO difference between G4s and G3s!!
I hope Mario is correct when he says that more apps are to be made
'Altivec aware', starting with the software that about 90% of BD users
require, i.e., Cellquest!!  Incidentally Mario, the G4 Cube does not and
probably never will contain (for cooling reasons) dual G4 processors.

Thereafter, Paul himself weighed in with estimates of Apple's market
share at 4%, (a later post reduced this even further).  Regardless of
whether these numbers came from the 'Microsoft Book of Facts'
(apparently a very thin volume indeed) or other reputable sources, the
point is irrelevant, even if Apple did sell to all areas of the World
(which it does not).  Whether Apple has 12% of the US market, or 40% of
the K12 education market in the US, or 45% of our Research Institution,
any other market share, is also irrelevant.  The one relevant statistic
for me is that of the 4 sorters and 6 bench-top cytometers in our
'Research Flow lab', 80% of them are running Macs and Cellquest.  I
suspect similar situations can be found elsewhere.  I do not think we
need to get into 'Mac vs PC' or 'BD versus Coulter' stuff.  DIVERSITY IS
IMPORTANT!!

While BD have taken a bit of flak over their failure to provide timely
upgrades to the software that most BD/Mac combinations run on, it is my
understanding that the software at the heart of the Coulter operating
system is still (for almost all users) DOS based.  It was not until the
recent (I think) availability of 'EXPO' software, that you could even
run Windoze on an XL/PC combo.  (I suspect the ability to do the latter
might not be seen by some as a step forward).  Even so, we regularly
take listmode files from my collaborators (who have a Coulter/PC set-up)
and publish them in color via FACSConvert and Cellquest on my Mac.  At
the end of the day, the 'Figure' is simply superior.

Regardless, without both manufacturers competing for sales, things would
be far far worse for us consumers.  Its the same with Mac versus PC.
Whether you use either or both platforms, only knuckleheads would
disagree that DIVERSITY IS IMPORTANT!!  After all, without Apple and
others, who would Micro$oft get its ideas from?  Like Karim, who stated
"I've worked on both platforms and believe me I wouldn't even think
about doing my FACS work (or any other daily lab tasks) on an NT
machine", I hope BD continue to support the Mac OS (and improve it!).

Finally, in his latest post, Paul asks 'how long will the Mac be
around?'  I do not think that the 'pin-striped reptiles of Wall Street'
should be influencing your short and medium term purchasing decisions.
Did you also notice that Intel was down over 50% from its yearly high,
Dell too, Micro$oft too, etc., etc.  Has anyone on Wall Street 'written
off' these companies?  Of course not.  What happened that made Apple's
previous valuation of $17.39 billion suddenly drop to $8.4 billion? As
detailed elsewhere
(http://macweek.zdnet.com/2000/10/01/1005everywallst.html) Apple only
made about $1.9 billion this year, instead of just over $2 billion It
only grew about 20 percent this year, instead of a little more. "Oooh,
scary stuff -- the company is doomed, doomed I say!  Sell now while you
still can.  This crosses the line from stupidity into insanity".
Lets support diversity (and keep G4 Cubes out of Mario's flow lab, - I
hate it when coffee gets spilled into the CD-Rom reader!!).

My very last 2c worth.

Rob Sutherland
University Health Network
University of Toronto
Received on Tue Oct 10 11:29:07 2000
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G4 for FACSCalibur - and a plug for Mario!!
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From: Reed, Doug S Dr USAMRIID <Doug.Reed@DET.AMEDD.ARMY.MIL>
Date: Tue Oct 10 2000 - 14:28:11 EST
Having read the great PC vs Mac debate going on here, I thought I'd throw in
my two cents worth on the whole matter. The whole issue of who's computer is
best comes down (really) to personal preferences.

Personally I have never understood the appeal of a Mac, and I've owned
computers since the heyday of the Trash System 80s. But to each his or her
own. It would certainly be nice if BD were to offer its customers the option
of Windows, Mac, BeOS or Linux, but that seems unlikely in the extreme. I
seriously doubt BD would invest the development time required, because the
expense would not justify it. And don't hold out for a Java-based CellQuest
- the list of Java applications that "run anywhere" are very few indeed.
Java may someday be capable of such feats, but for now it is far to sluggish
to handle real-time acquisition on a cytometer.

The biggest advantage to me for using a Mac instead of a PC on the Calibur
is that no one (here anyways) will be using it for anything other than FACS.
:^)

Douglas S. Reed, Ph.D.
Department of Aerobiology and Product Evaluation
Division of Toxinology & Aerobiology
U.S. Army Medical Research Institute of Infectious Diseases
1425 Porter St. Ft. Detrick
Frederick, MD 21702
(301) 619-6728 voice
(301) 619-2541 fax
doug.reed@det.amedd.army.mil
Received on Wed Oct 11 13:03:32 2000
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Re: Mac Notebooks for Cellquest
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From: Adrian Smith <A.Smith@centenary.usyd.edu.AU>
Date: Tue Nov 14 2000 - 19:07:27 EST
>We are interested in buying a MAC for off-line analysis.  Since we have a
>shortage of desk/bench space, there is interest in perhaps getting a
[quoted text clipped - 8 lines]
>
>Doug

If you are going to buy another Macintosh specifically for analysis
then you will need to buy another license for CellQuest. In that case
before you buy make sure you consider FlowJo as an alternative
analysis package to CellQuest. We have found it much better and there
have never been any compatibility problems - I'm running it right now
on a Powerbook. I have not tried CellQuest on a Powerbook. (All our
acquisition is done on a FacStarPlus with a Mac running CellQuest,
but I haven't used CellQuest for analysis since FlowJo was released
in 1997).

In terms of saving desk/bench space, you might also consider the G4
Cube - it really is a tiny computer and if you coupled it with an LCD
monitor it would take up not much more space than a laptop (I have no
idea how that would compare price wise though - in Australia it would
end up being about the same I think). The Cube will run FlowJo
slightly faster but there is not a big difference between a G4/400
(Cube) or the G3/500 in the top of the line Powerbook (rumour has it
that the Powerbook line will be significantly revised in January if
you can wait that long...).

The big disadvantage of going small (whether laptop or lcd monitor)
is the limited screen space you can use. Both CellQuest and FlowJo
benefit from more screen space, ie larger monitors. However, the best
way I have found to increase screen space is to add a second monitor.
The Powerbook has the advantage here as it supports a second monitor
straight out of the box - at the moment I'm running the powerbook
with a 15inch monitor sitting beside it and the Mac desktop expands
over both of them. (It is more difficult to add a second monitor to
the Cube as it involves finding a single video card that will support
dual monitors; if you bought the normal G4 Tower you would simply add
a second PCI video card) Of course once you start adding second
monitors they start taking up more space.

The other thing to make sure is that you get sufficient RAM. Some of
the base Mac models ship with only 64MB. This may be enough depending
on the analysis you do but I think 128MB or more is much better (our
lab G4s which are used for analysis have 192MB).

Don't hesitate to ask any more questions...

Adrian Smith
Received on Wed Nov 15 11:32:16 2000
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Re: Mac Notebooks for Cellquest
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From: Karim Vermaelen <Karim.Vermaelen@rug.ac.be>
Date: Thu Nov 16 2000 - 08:49:27 EST
Hi,
Like Adrian, I've had good experience doing intensive off-line analysis of
flow
data on a Powerbook G3 laptop running FlowJo (check out www.treestar.com for
prices and licensing options; you can get a free limited-time demo license
for a
test-drive). The FlowJo license we bought currently  runs Flowjo 2.7.8 on a
266
Mhz "beige" desktop G3, under MacOS 9.0.4 and multiple-users ON (OSX is still
in
beta testing phase and neither Cellquest nor FlowJo are native OSX
applications
today).
I often have to acquire >100000 events/5 parameters per sample for my
experiments but the analysis really flies on this G3, and it's even snappier
on
my 300 Mhz G3 laptop.  I even (BD's gonna hate that) performed event
acquisition
straight  from the FACStation (an old upgraded Quadra) to the Powerbook over
Ethernet. With the new G4 tower FACStations and Powerbooks (besides more
processing power) you have the added option of wireless networking for
transfer/backup of data after acquisition. Check out www.apple.com/airport
for a
clear explanation.
As for the PC issue, our dept. is primarily a PC network with a file server
running Novell Netware. Netware can talk to Appletalk networks through a
user-transparent interface on the Mac side (NT provides this too, albeit
slower
as far as I've heard). We used to back up gigs of FCM data from the Mac
network
to a PC file server without any problem.
Feel free to mail for any additional questions
Karim

> >We are interested in buying a MAC for off-line analysis.  Since we have a
> >shortage of desk/bench space, there is interest in perhaps getting a
[quoted text clipped - 8 lines]
> >
> >Doug

Received on Thu Nov 16 13:32:15 2000
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>ISAC XXII International Congress & Purdue Cytometry University ML &
>XML
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>
>http://www.new.facebook.com/note.php?note_id=23879772873&ref=mf
 
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