Medical Forum / Diseases and Disorders / AIDS / December 2008
ISAC XXII International Congress & Purdue Cytometry University ML & XML Data Standards Committee Concludes New FCS 4.0 Commercial Software Development? License Fees Could go UP!
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Mitch Haynes - 27 Aug 2008 04:35 GMT ISAC XXII International Congress & Purdue Cytometry University ML & XML 1993-2008 Commercial Data Storage,J Paul Robison & Crew
Purdue Cytometry Mailing List: 3rd party sw... party flow cytometry software vendors are:
Verity Software House, Inc. 10 New ... CO 80525 800 822 9902 voice 303 226 2322 fax
TrueFacts Software, Inc. ... www.cyto.purdue.edu/hmarchiv/1993/0046.htm - 5k - Cached - Similar pages - Note this
Purdue Cytometry Mailing List: a tractor and other farm toolsOne final point on software. As some have noted in past messages, the best software has come from independent providers
(Verity, Phoenix, TrueFacts, ...
www.cyto.purdue.edu/hmarchiv/1994/0293.htm - 7k - Cached - Similar pages - Note this
FlowJo is Yet to come!
Will the standard be Open? Will the software be licensed? Will we be forced to redevelop or Software. Have they already Deveolped New software since they have been planing to change the standard to their own for some time now?
My client Phoenix Flow has a product, QC-Tracker, which can be transformed into a generic FCS reader, data storage system, and user programmable data analysis system. Would you or others be interested
Phoenix Flow Systems 11575 Sorrento Valley Road, Suite 208 San Diego, CA 92121 619 453 5095 voice 619 259 5268 fax
In the study of economics and market competition, collusion takes place within an industry when rival companies cooperate for their mutual benefit. Collusion most often takes place within the market form of oligopoly, where the decision of a few firms to collude can significantly impact the market as a whole. Cartels are a special case of explicit collusion. Collusion which is not overt, on the other hand, is known as tacit collusion.
Photonics: Principles and Practices - Google Books Resultby Abdul Al- Azzawi - 2006 - Science - 968 pages
Newport Corporation. Optics and mechanics. Newport 1999/2000 catalog, ... Robinson. Paul, Laboratory Manual to Accompany Conceptual Phvsics. ...
books.google.com/books?isbn=0849382904...
Purdue Cytometry Mailing List: RE: EPICS C dataFrom: J.Paul Robinson (j...@flowcyt.cyto.purdue.edu) ... From: Suzanne Leif > President of Newport Instruments > Via Robert C. Leif, Ph.D. > Vice President ...
www.cyto.purdue.edu/hmarchiv/1998/1995.htm - 7k - Cached - Similar pages - Note this
Dayong JinJingli Yuan’s group),
Newport Instruments
(Dr. Robert Leif’s group) and
Purdue University Cytometry Labs
(Prof. J.Paul Robinson’s group). ... www.ics.mq.edu.au/gen/person/jin.html - 13k - Cached - Similar pages - Note this
Data and Image Analysis Special Interest Group Meeting 2007J. Paul Robinson, SVM Professor of Cytomics, Purdue University and President, ... (*) Robert C. Leif, Newport Instruments. "Cytometry Standards Continuum" ... www.ravkin.net/SBS/D&IA_SIG2007.htm - 14k - Cached - Similar pages - Note this
[PPT] DIA SIG 2007: What are the Issues?File Format: Microsoft Powerpoint - View as HTML ... J. Paul Robinson, President, International Society for Analytical Cytology, ... (*) Robert C. Leif, Newport Instruments "Cytometry Standards Continuum" ... www.ravkin.net/SBS/DIA%20SIG%202007%20Intro.ppt - Similar pages - Note this
The Science Advisory Board - Protocols, Product Reviews, Member ...Flow cytometry started as a simple instrument designed during World War II to ... Dr. J. Paul Robinson, Purdue University School of Veterinary Medicine, ... www.scienceboard.net/community/perspectives.169.html - 30k - Cached - Similar pages - Note this
Wiley Cytometry - ISAC 2000 International Congress POSTER ABSTRACTSStephanie Ann Sincock, Purdue University; J.Paul Robinson, Purdue University ...... Robert Leif, Newport Instruments; John Quagliano, Los Alamos National ... www.wiley.com/legacy/products/subject/life/cytometry/isac2000/posters... - 223k - Cached - Similar pages - Note this
Verity Software House, Inc. 10 New Lewiston Road Topsham, Maine 04086 207 729 6767 voice 207 729 5443 fax
Phoenix Flow Systems 11575 Sorrento Valley Road, Suite 208 San Diego, CA 92121 619 453 5095 voice 619 259 5268 fax
Cytomation, Inc. 400 E. Horsetooth Rd., Suite 100 Fort Collins, CO 80525 800 822 9902 voice 303 226 2322 fax NEW ADDRESS FORT COLLINS DRIVE
Purdue Cytometry Mailing List: Cytomation3- Our new address- Cytomation, Inc. 4850 Innovation Drive Fort Collins, CO 80525 4- Our service, support and telephone availability will not change during ... www.cyto.purdue.edu/hmarchiv/1998/2730.htm - 7k - Cached - Similar pages - Note this More results from www.cyto.purdue.edu »
TrueFacts Software, Inc. 1011 Boren Ave., Suite 193 Seattle, WA 98104 800 252 5248 voice 206 621 9665 fax Robert C. Leif and Suzanne B. Leif Ada-Med/Newport Instruments (USA)
The International Society for Analytical Cytology, ISAC, has developed a flow cytometry standard (FCS) to permit data interchange, ISAC will soon replace FCS 2.0 with FCS 3.0. Unfortunately, the proposed FCS 3.0 is still fraught with problems, which are of sufficient magnitude as to warrant its early replacement.
The most reasonable replacement is as a supplement to the digital imaging and communications in medicine, DICOM 3.0, standard.
The recent digital microscopy extension of DICOM can be extended and modified to include flow cytometry data. DICOM includes: image graphics objects, specifications for describing: studies, reports, the acquisition of the data and the individuals involved, physician, patient, etc. Storing the present FCS data in a database, which has already been accomplished with the QC tracker software, will facilitate the transition of FCS to DICOM.
©2005 COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Purdue Cytometry Mailing List: Re: Re[2]: Leave FCS3.0 alone.
My client Phoenix Flow has a product, QC-Tracker, which can be transformed into a generic FCS reader, data storage system, and user programmable data ... www.cyto.purdue.edu/hmarchiv/1997/1762.htm - 11k - Cached - Similar pages - Note this
From: Robert C. Leif, Ph.D. (rl...@rleif.com) Date: Tue Jul 29 1997 - 20:25:22 EST * Next message: Vincent Falco: "Salary Survey Responses" * Previous message: Maryalice Stetler-Stevenson: "Re: CD20 Gating" * Maybe in reply to: Jim Houston: "Re: Re[2]: Leave FCS3.0 alone." * Next in thread: Dave Coder: "Re: Re[2]: Is FCS3.0 obsolete?" * Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ] ________________________________________ To: cyto-inbox From: Bob Leif
Please see Suzanne Leif's and my posting on the ISAC web site and R. C. Leif and S. B. Leif, "Evolution of Flow Cytometry Standard, FCS3.0, into a DICOM-Compatible Format". Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology, Ed. A. V. Priezzhev , T. Asakura, and R. C. Leif. A. Katzir Series Editor, Progress Biomedical Optics Series , SPIE Proceedings Series,Vol. 2982, pp 354-366 (1997).
Many of your very good suggestions were separately arrived at by us. However, there are two separate subjects: 1) What should be included in a Flow Cytometry File for Data Transfer and 2) The actual format for the Flow Cytometry File for Data Transfer. We suggested switching to the Digital Imaging and Communications in Medicine, DICOM, format after the year 2000.
My client Phoenix Flow has a product, QC-Tracker, which can be transformed into a generic FCS reader, data storage system, and user programmable data analysis system. Would you or others be interested in this type of product?
Purdue Cytometry Mailing List: RE: Creating a database of FCS fiThe product was QCTracker from Phoenix Flow Systems. Experience with the development of that product was one of the reasons for the creation of CytometryML. ... www.cyto.purdue.edu/hmarchiv/2004/0786.htm - 8k - Cached - Similar pages - Note this ANYONE HAS AN EXPERIENCE TO WORK WITH WIN-FCM AND OX9 CONTROL SOFTWARE
Purdue Cytometry Mailing List: Phoenix Flow SystemsDoes anyone has an experience to work with Win-FCM Interface Box and OS9 interface control software from PHOENIX Flow Systems?
We are going to get one for ... www.cyto.purdue.edu/hmarchiv/1997/0271.htm - 5k - Cached - Similar pages - Note this More results from www.cyto.purdue.edu »
Penn SDM Flow Cytometry Facility
Flow Cytometry at Purdue University; The Purdue links are excellent starting ... BrdU-based TUNEL Assay from Phoenix Flow plus a Cell Fixation Procedure ...
Map of 4010 Locust St, Philadelphia, PA 19104
biochem.dental.upenn.edu/~sdmfacs/ - 14k - Cached - Similar pages - Note this
(Cytomation, Coulter, Verity, Phoenix Flow, and MSA, not B-D) NOT B-D
Purdue Cytometry Mailing List: Re: Histogram SubtractionThis method is available in most commercial flow software (Cytomation, Coulter, Verity, Phoenix Flow, and MSA, not B-D). In the years since then, ... www.cyto.purdue.edu/hmarchiv/1998/1740.htm - 8k - Cached - Similar pages - Note this
[PDF] Great Lakes International Imaging and Flow Cytometry AssociationFile Format: PDF/Adobe Acrobat - View as HTML
Cytomation, Inc. DAKO Corporation.
Flow Cytometry Standards Corporation ... Karen Cornell, Purdue University, West Lafayette, IN, “Flow. Cytometric and ... www.gliifca.org/pdf/GLIIFCA-1995.pdf - Similar pages - Note this
THANKS FOR A SUCCESSFUL 2007
Purdue Cytometry Mail list-Cytometry Education Associates, Inc ...NW Regional ... ... Purdue Cytometry Mailing List: RE: Thanks to all for a successful 2007 NW Regional ... Miltenyi, Phoenix Flow Systems, Spherotech, Tree ... groups.google.co.uk/group/misc.health.aids/browse_thread/thread/ a7d391d007a402e7 - 179k - Cached - Similar pages - Note this More results from groups.google.co.uk »
From: Robert C. Leif <rl...@rleif.com>The FCS header files have already been parsed and stored in a database(1). The product was QCTracker from Phoenix Flow Systems.
RE: Creating a database of FCS files
* This message: [ Message body ] [ More options ] * Related messages: [ Next message ] [ Previous message ] [ In reply to ]
From: Robert C. Leif <rl...@rleif.com> Date: Sat Apr 24 2004 - 11:57:17 EST
The FCS header files have already been parsed and stored in a database(1). The product was QCTracker from Phoenix Flow Systems.
Experience with the development of that product was one of the reasons for the creation of CytometryML. Since the data in a CytometryML file is all validated XML, it can be imported without any changes into commercially available databases, spreadsheets and other applications. The list mode data and associated index files are stored as a simple array of records(structs), which can be read by commercially available common programming languages or manipulated by .Net object.
1. R. C. Leif, R. Rios, M. C. Becker, C. K. Becker, J. T. Self, and S. B. Leif, "The Creation of a Laboratory Instrument Quality Monitoring System with AdaSAGE". Advanced Techniques in Analytical Cytology, Optical Diagnosis of Living Cells and Biofluids, Ed. T. Askura, D. L. Farkas, R. C. Leif, A. V. Priezzhev, , and B. J. Tromberg.. A. Katzir Progress in Biomedical Optics Series Editor SPIE Proceedings Series, Vol. 2678, 232-239 (1996).
2. R. C. Leif, S. B. Leif, and S. H. Leif, "CytometryML, An XML Format based on DICOM for Analytical Cytology Data ", Cytometry 54A pp. 56-65 (2003).
3. R.C. Leif, S.H. Leif, S.B. Leif, CytometryML, a markup language for analytical cytology, in Manipulation and Analysis of Biomolecules, Cells and Tissues, D. V. Nicolau, J. Enderlein, and R. C. Leif, Editors, SPIE Proceedings Vol. 4962 pp 288-297 (2003).
-----Original Message----- From: Adrian Smith [mailto:A.Sm...@centenary.usyd.edu.au] Sent: Thursday, April 22, 2004 6:57 PM To: cyto-inbox Subject: Creating a database of FCS files
Hi all,
Some of the users here have raised the desirability of having a database of all the FCS headers from all their data files. They could then, for example, search for all the files/experiments in which they used a particular stain etc.
Is anybody doing this? Would this be something that other people would find useful?
I would love to set something up but I don't have the requisite skills or time at the moment.
As a temporary measure I suggested they export the FCS header info from FlowJo using using a table and then compile them all in another program like excel. This works for a few experiments but it needs to be automated (and easy) if it is going to be generally applicable.
Any comments or suggestions?
Adrian Smith Centenary Institute of Cancer Medicine and Cell Biology Sydney, Australia
Received on Mon Apr 26 14:38:00 2004
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RE: Phoenix software for Coulter analyser From: Dan Smith (D...@ALLP.COM) Date: Thu Jan 30 1997 - 16:48:00 EST • Next message: Pizzo,Eugene: "FACSCalibur 4-color option" • Previous message: Pete Macardle: "(no subject)" • Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ] ________________________________________ Phoenix Flow's multiplus has worked very well in analyzing Profile II data for us. Dan Smith San Diego ---------- {From: owner-cyto-sendout {To: Cytometry Mailing List {Subject: Phoenix sofware for Coulter analyser {Date: Thursday, January 30, 1997 4:28PM { { {We are looking into software for good display and analysis of data. {We use a coulter profile 2 analyser. Does anyone have experience with {Phoenix MultiPlus analysis software, or could recommend any other {software package for consideration? { {I would appreciate any advice. { {Stan.Stan Ress {Clinical Immunology laboratory {Dept of Medicine {H47 Old main BLG, {Groote Schuur Hospital, {Observatory, {Cape Town, South Africa {7925 { {e-mail: sr...@uctgsh1.uct.ac.za {Phone: Intern + 2721-4066201 {FAX : Intern + 2721-4486815 { ________________________________________ • Next message: Pizzo,Eugene: "FACSCalibur 4-color option" • Previous message: Pete Macardle: "(no subject)" • Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ] ________________________________________ This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:33:51 EST
From: Robert C. Leif, Ph.D. (rl...@rleif.com) Date: Tue Jul 29 1997 - 20:25:22 EST * Next message: Vincent Falco: "Salary Survey Responses" * Previous message: Maryalice Stetler-Stevenson: "Re: CD20 Gating" * Maybe in reply to: Jim Houston: "Re: Re[2]: Leave FCS3.0 alone." * Next in thread: Dave Coder: "Re: Re[2]: Is FCS3.0 obsolete?" * Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ] ________________________________________ To: cyto-inbox From: Bob Leif
Please see Suzanne Leif's and my posting on the ISAC web site and R. C. Leif and S. B. Leif, "Evolution of Flow Cytometry Standard, FCS3.0, into a DICOM-Compatible Format". Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology, Ed. A. V. Priezzhev , T. Asakura, and R. C. Leif. A. Katzir Series Editor, Progress Biomedical Optics Series , SPIE Proceedings Series,Vol. 2982, pp 354-366 (1997).
Many of your very good suggestions were separately arrived at by us. However, there are two separate subjects: 1) What should be included in a Flow Cytometry File for Data Transfer and 2) The actual format for the Flow Cytometry File for Data Transfer. We suggested switching to the Digital Imaging and Communications in Medicine, DICOM, format after the year 2000.
My client Phoenix Flow has a product, QC-Tracker, which can be transformed into a generic FCS reader, data storage system, and user programmable data analysis system. Would you or others be interested
Purdue Cytometry Mailing List: Cytomation3- Our new address- Cytomation, Inc. 4850 Innovation Drive Fort Collins, CO 80525 4- Our service, support and telephone availability will not change during ... www.cyto.purdue.edu/hmarchiv/1998/2730.htm - 7k - Cached - Similar pages - Note this More results from www.cyto.purdue.edu »
Cytomation Inc.'s CyCLOPS softare. Data acquired on the Coulter Epics ..... Purdue University Cytometry Laboratory.
Cytomation Inc.'s CyCLOPS softare. Data acquired on the Coulter Epics ..... Purdue University Cytometry Laboratory.
[PDF] MFI: a flow cytometry list mode data analysis program optimized ...File Format: PDF/Adobe Acrobat - View as HTML Cytomation Inc.'s CyCLOPS softare. Data acquired on the Coulter Epics ..... Purdue University Cytometry Laboratory. I am grateful to the National Science ... www.umass.edu/microbio/mfi/mfims.pdf - Similar pages - Note this
Purdue Cytometry WinMDI software Fails on LM-files contact Verity ...Purdue Cytometry WinMDI software Fails on LM-files contact Verity House Software .... interpret CyCLOPS files provided by Cytomation, Inc. (303-226-2200, ...
groups.google.com/group/misc.health.aids/browse_thread/thread/ 7410e3c6def72b16 - 51k -
The Wistar Institute :: Flow Cytometry FacilityBasics of High-Speed Cell Sorting on the Cytomation MoFlo .... Samples were analyzed using a DakoCytomation MoFlo (DakoCytomation, Inc., Fort Collins, CO) ... www.wistar.org/research_facilities/facilities/flowcytometry/links.html - 31k - Cached - Similar pages - Note this
____________________________The Science Advisory Board - Protocols, Product Reviews, Member ...- 57 visits - Jun 6Dr. J. Paul Robinson, Purdue University School of Veterinary Medicine, .... Beckman- Coulter, or Cytomation (Dako) instruments- check the website for the ... www.scienceboard.net/community/perspectives.169.html - 30k - Cached - Similar pages - Note this
Purdue Frederick Co. under the trademark "ALFERON" (Norwalk, ...... When using the preferred flow cytometer and software, the FACScan running the ... www.wipo.int/pctdb/en/wo.jsp?IA=WO1997019189&DISPLAY=DESC - 167k -
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PDF] CURRENT PROTOCOLS IN CYTOMETRYFile Format: PDF/Adobe Acrobat - View as HTML CYTOMETRY. EDITORIALBOARD. J. Paul Robinson, Managing Editor. Purdue University ...... CYTOPC: Processing software for. flow cytometric data. ... www.sb-roscoff.fr/Phyto/Reprints/Marie_et_al_CPC_00.pdf - Similar pages - Note this
[PDF] CURRENT PROTOCOLS IN CYTOMETRYFile Format: PDF/Adobe Acrobat - View as HTML Purdue University. West Lafayette, Indiana. Zbigniew Darzynkiewicz ...... CYTOPC: Processing software for. flow cytometric data. Signal Noise 2:8. ... www.sb-roscoff.fr/Phyto/Reprints/Marie_et_al_CPC_99.pdf - Similar pages - Note this
Great Lakes International Imaging and Flow Cytometry Association File Format: PDF/Adobe Acrobat - View as HTML Karen Cornell, Purdue University, West Lafayette, IN, “Flow. Cytometric and Image Analysis of ... Abe Schwartz, Flow Cytometry Standards Corp., San Juan, ... www.gliifca.org/pdf/GLIIFCA-1995.pdf - Similar pages - Note this
Bangs Laboratories, Inc. :: Microspheres Flow Cytometry Standards Corporation Joins Bangs Laboratories to Provide a Full ... May 2000, Fishers, IN -- Flow Cytometry Standards Corporation has joined ... www.bangslabs.com/company/press.php?whichrelease=5 - 11k - Cached - Similar pages - Note this
Commercial Flow Cytometry Links Last Updated: 2006-09-07 See also: Salk Flow Cytometry Lab Bioscience Links. ... Flow Cytometry Standards Corp, San Juan, PR, [Acquired by Bangs Labs] ... flowcyt.salk.edu/comflow.html - 14k - Cached - Similar pages - Note this
Development of clinical standards for flow cytometry -- Schwartz ... Flow Cytometry Standards Corporation, Research Triangle Park, North Carolina 27709. Flow cytometers are instruments that can determine multiparameter data ... www.annalsnyas.org/cgi/content/abstract/677/1/28 - Similar pages - Note this
Flow cytometry Kina, and J. Paul Robinson, Purdue University Cytometry Laboratories and .... Stelzert and C. Stewart**, Flow Cytometry Standards Corporation*, ... doi.wiley.com/10.1002/cyto.990140907 - Similar pages - Note this
[PPT] A Unique Opportunity in Biological Information Standards C. Forbes ...File Format: Microsoft Powerpoint - View as HTML
Flow Cytometry / FACS; Microarray Experiments; Mass Spectrometry; Microscope Images ... --Robert C. Leif, Suzanne B. Leif, et al., XML_Med, a Division of ... www.w3.org/2004/10/swls/forbes_dewey.ppt - Similar pages - Note this
Increasing the luminescence of lanthanide complexesment Funds, Lidia Vallarino’s Gift Fund,
and Phoenix Flow Systems Internal ...... 66. Leif RC. CytometryML binary data standards. Proc SPIE 2005;5699: .
.. doi.wiley.com/10.1002/cyto.a.20321 - Similar pages - Note this More results from doi.wiley.com »
[PDF] Increasing the Luminescence of Lanthanide(III) Macrocyclic ComplexesFile Format: PDF/Adobe Acrobat - View as HTML S.A. Williams. c. , and S. Yang. a. a. Newport Instruments, San Diego, CA 92115-1022, USA. b. Phoenix Flow Systems, San Diego, CA 92121-1319 ..
. www7.nationalacademies.org/bpa/COHMAG_Input_Leif2.pdf - Similar pages - Note this
by RC Leif - Cited by 3 - Related articles - All 9 versions
Defense Technical Information Center Compilation Part NoticeR. C. Leif, Methods for Preparing Sorted Cells as Monolayer Specimens, In Living Color, Protocols in Flow Cytometry and Cell. Sorting, R. A. Diamond and S. ... www.stormingmedia.us/68/6889/P688910.pdf - Similar pages - Note this by RC Leif - 2004
[PDF] CytometryML, a markup language for analytical cytologyFile Format: PDF/Adobe Acrobat - View as HTML
XML_Med, a Division of Newport Instruments, 5
648 Toyon Road, San Diego, ...... R. C. Leif and S. B. Leif, "Evolution of Flow Cytometry Standard, FCS3.0, ... chemdye.com/cytometryml/pdf/CytometryML_SPIE03.pdf - Similar pages - Note this
[PDF] Nov2005: Abstracts: George L. Wied Memorial SymposiumFile Format: PDF/Adobe Acrobat
Robert Leif, Ph.D., P.M.I.A.C.. Objective: Cytology automation will be enhanced by the ... croscopy and flow cytometry can be created in a manner ...
www.acta-cytol.com/admin/wiedsymposiumabs.pdf - Similar pages - Note this
Reagent system and method for modifying the luminescence of ...(k) A pair of Leif Centrifugal Cytology Buckets (Ref. 33)
(Newport Instruments) that ..... 35) described in the
Phoenix Flow Systems ABSOLUTE-S™ Kit (Ref. ...
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(WO/2005/046735) A REAGENT SYSTEM AND METHOD FOR MODIFYING THE ...(k) A pair of Leif Centrifugal Cytology Buckets (Ref. 33) (Newport Instruments) that ..... Parallel flow cytometry measure- ments with fluorescein labeled ... www.wipo.int/pctdb/en/wo.jsp?IA=US2004037314&DISPLAY=DESC - 841k - Cached - Similar pages - Note this
Wiley Cytometry - ISAC 2000 International Congress POSTER ABSTRACTSStephanie Ann Sincock,
Purdue University; J.Paul Robinson, Purdue University ...... Robert Leif, Newport
Instruments; John Quagliano, Los Alamos National ... www.wiley.com/legacy/products/subject/life/cytometry/isac2000/posters...
ISAC XXII International CongressPhoenix Flow Systems, Newport Instruments R&D, San Diego, ...... J. Paul Robinson. 6. 1. Purdue University, Electrical and Computing Engineering, West ... doi.wiley.com/10.1002/cyto.a.20045.abs - Similar pages - Note this by P Session
ISAC XXII International CongressPhoenix Flow Systems, Newport Instruments
Data and Image Analysis Special Interest Group Meeting 2007
J. Paul Robinson, SVM Professor of Cytomics,
Purdue University and President, ... (*) Robert C. Leif, Newport Instruments.
Cytometry Standards Continuum" ...
www.ravkin.net/SBS/D&IA_SIG2007.htm - 14k - Cached - Similar pages - Note this
AbstractsSilas J. Leavesley1,
Bartlomiej Rajwa2,
J. Paul Robinson3 1Purdue ...... R&D, San Diego, California;
2Phoenix Flow Systems,
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www.cyto.purdue.edu/flowcyt/isac2006/abstracts.htm - 977k - Cached - Similar pages - Note this
[PDF] Call for Papers
File Format: PDF/Adobe Acrobat - View as HTML Sinai Medical Ctr.; Dan V. Nicolau, The Univ. of Liverpool (United Kingdom);
Robert C. Leif,. Newport Instruments. Cochairs: J. Paul Robinson, Purdue Univ.; ...
spie.org/Documents/ConferencesExhibitions/Photonics-West-2009-BiOS- Call.pdf - Similar pages - Note this
Flow cytometry
Kina, and J. Paul Robinson,
Purdue University Cytometry Laboratories
and ....
Stelzert and C. Stewart**,
Flow Cytometry Standards Corporation*, ...
doi.wiley.com/10.1002/cyto.990140907 - Similar pages - Note this
[PDF]
MFI: a flow cytometry list mode data analysis program optimized ... File Format: PDF/Adobe Acrobat - View as HTML
(#3, Flow Cytometry Standards Corp., POB 12621,
Research Triangle Park .....
Purdue University Cytometry Laboratory.\
I am grateful to the National Science ...
www.umass.edu/microbio/mfi/mfims.pdf - Similar pages - Note this
Commercial Flow Cytometry Links
Last Updated: 2006-09-07 See also:
Salk Flow Cytometry Lab Bioscience Links. ..
. Flow Cytometry Standards Corp
, San Juan, PR, [Acquired by Bangs Labs] ...
flowcyt.salk.edu/comflow.html - 14k - Cached - Similar pages - Note this
Purdue Cytometry Mailing List: RE: Report from
ISAC Meeting San Diego
Thanks to Phoenix Flow Systems,
Guava Technologies,
PROzyme and Becton ...
Adam Treister Tree Star, Inc.
ph: 800-366-6045 intl: 1-650-591-2854 fax: ...
Address any questions, comments, or suggestions to
Mario Roederer.
www.cyto.purdue.edu/hmarchiv/2002/1065.htm - 8k - Cached - Similar pages - Note this
Sunday, July 13, 2008 ISAC Congress Biological Councilor 2008 has UR FlowJo Month ISAC CONGRESS Biological Councilor 2008 has UR FlowJo Month
Highlights of the XXII 2004 ISAC Congress
Thanks from the Coalition for Change team!
6 of the 7 members of our team are now
on ISAC Council,
including the new
President Elect (J.Paul Robinson) &
Secretary (Bob Zucker).
Data Standards Committee!
Apple - Science - Profiles - Dr. Mario Roederer Dr. Mario Roederer. In a major new revision for Mac OS X, FlowJo 6 brings ... Roederer and Treister developed FlowJo for the Mac first because one of the ...www.apple.com/science/profiles/roederer/ - 17k - Cached - Similar pages - Note this
Bushnell, Timothy Mambo Mar 6, 2008 ISAC Election Biological Councilor.
Disclaimer: from the Purdue Cytometry Mail List ARCHIVE
Yes, I live off FlowJo sales, and that's a blatantly commercial statement, but it gets technical from here on.]
http://www.cyto.purdue.edu/hmarchiv/2006... - 8.5KB
On Aug 20, 2004, at 2:25 PM,
J. Paul Robinson wrote:
ummm....Mario says.. "In life sciences -- particularly in research life sciences --> probably 50 to 70% of research laboratories used Macs".... while I have a passionate dislike for Windows...... is this really true ??? or is the key word there "used"??
(Ok...I have put on my helmet and armor....waiting...) paul
For all of the mr groupies out there in cytometry cyberspace. Don't wet your pocket protectors over this.
Honestly though, well deserved praise for Mario & the Tree Star group
http://www.apple.com/science/profiles/ro...
FlowJo MONTH at UR CPBR Flow Lab University of Rochester Office.
October 2007 Options:Show Author Hide Author Show Table of Contents Hide Table of Contents Join or Leave UR_CYTOMETRYSearch ArchivesSubject
From Date Size2nd call - Fall Flowjo order Bushnell, Timothy
Tue, 9 Oct 2007 09:30:09 -0400309 linesFall Flowjo order Bushnell, Timothy
Mon, 1 Oct 2007 09:30:27 -0400242 linesFlocyte regional training program Bushnell, Timothy
Wed, 31 Oct 2007 14:30:22 -0400221 linesLast call - Fall Flowjo order Bushnell, Timothy
Thu, 25 Oct 2007 08:15:20 -0400231 linesReminder: 1 pm today: Flow Cytometry Lecture Series. Bushnell, Timothy
Thu, 11 Oct 2007 09:19:48 -0400214 linesToday is the last day to join the Flowjo bulk order Bushnell, Timothy
Tue, 23 Oct 2007 09:23:59 -0400341 linesBack to:
Main UR_CYTOMETRY Page
Bushnell, Timothy Date:Fri, 11 Jan 2008 10:31:11 -0500
Colleagues: Dr. John Quinn, applications scientist from Treestar, will be presenting web based seminar Friday January 11th at 1 pm on KMRB 3-9624 on Compensation and Transformation All are welcome. Regards Tim Timothy Bushnell, Ph.D.Research Assistant Professor, Pediatrics and Oncology Co-Director, URMC Flow Cytometry FacilityOffice: 585-273-5535Lab: 585-273-1361Fax: 585-276-0233http://www.urmc.rochester.edu/Aab/ genepe...
Subject:Reminder: Today 1 pm - Flowjo Seminar on Compensation and Transformation From:"Bushnell, Timothy" October 2007
Thanks > > Tim > > > > Timothy Bushnell, Ph.D. > > Research Assistant Professor, ... Justin, We're working hard to finalize FlowJo Collectors' Edition, ...mycyte.org/index.php?option=com_newsfe... - 133k - Cached - Similar pages - Tree StarFile Format: PDF/Adobe Acrobat - View as HTMLData Analysis/ Presentation – Tim Bushnell & Ryan Duggan ..... Purdue University Cytometry Laboratories, West Lafayette, IN ...www.gliifca.org/pdf/ GLIIFCA-2004.pdf -
Purdue Cytometry Mailing List: FW: Second Announcment:WNYFUG 2 From : Bushnell, Timothy ... is sponsored by Treestar, and the best poster will win a 1 year subscription to Flowjo! ...www.cyto.purdue.edu/hmarchiv/2006/1019... - 9k - Cached - Similar pages -
Purdue Cytometry Mailing List: By DateRE: FCS files to Excel with Diva or FlowJo Ashley Carter. Thu Mar 13 2008 - 18:25:41 EDT ..... New email list for Imagestream users Bushnell, Timothy ...www.cyto.purdue.edu/hmarchiv/Current/ - 248k - Cached - Similar pages - Note thisMore results from www.cyto.purdue.edu »
Flowjo purchase 2007 From:"Bushnell, Timothy" Reply-To:Cytometry Date:Tue, 3 Apr 2007 14:55:23 -0400 Colleagues: As you know, I have organized a yearly purchase for Flowjo, a flow cytometry data analysis package. If you are not familiar with this software, you can find information at http://www.flowjo.com/home/overview.html... A single seat (dongle/license) is $1325. We start to get additional discounts when we order more than 5 dongles.
I have been approached by several labs interested in purchasing new copies of the software, so I am starting to organize another bulk purchase for this spring. If your lab is interested please email me privately with the number of dongles you wish to purchase and an account number to charge the purchase.
I will start the paperwork on 16 April, so please get your information to 1 day ago me promptly. Regards, Tim Timothy Bushnell, Ph.D.Research Assistant Professor, Pediatrics and OncologyDirector, CPBR Flow LabUniversity of Rochester
http://www.urmc.rochester.edu/Aab/genepe... ____UR_Cytometry______Subscribers Corner_____ http://listserv.urmc.rochester.edu
Subject:Fall Flowjo order
From:"Bushnell, Timothy" Reply-To:Cytometry Bushnell, Timothy Date:Mon, 1 Oct 2007 09:30:27-0400
Colleagues: As in the past, I am organizing a bulk order for copies of the flow cytometry analysis software package Flowjo, produced by Treestar, Inc. You can find details on this software are www.flowjo.com. The base cost will be $1325 per copy + shipping ($18/#copies ordered).
I can usually arrange additional discounts when we order greater than 5 copies. If you are interested, please send me the number of copies you are interested in, the PI’s name, and an account number before October 15th.
Regards Tim Timothy Bushnell, Ph.D.Research Assistant Professor, Pediatrics and OncologyDirector, CPBR Flow LabUniversity
ISAC E-News -- June, 2007 - Mambo Jun 8, 2007 ... You also may contact the President, Dr. Tim Bushnell, ... Adam Treister introduced possibilities of integrating these with FlowJo. ...www.isac-net.org/content/view/593/119/ - 53k - Cached - Similar pages - Note this
ISAC E-NewsFile Format: PDF/Adobe Acrobat - View as HTMLthe President, Dr. Tim Bushnell, at Timothy_Bushnell@URMC.rochester.edu. ... introduced possibilities of integrating these with FlowJo. ...www.zangani.com/files/2007-0608-ISAC_E... - Similar pages - Note this
Subject:Reminder: Spring Flowjo order From:"Bushnell, Timothy" Date:Fri, 14 Mar 2008 12:21:24 -0400 Colleagues: Is your lab in need of a new flowjo dongle? I am again coordinating a bulk purchase of flowjo dongles. The academic price is $1495 a dongle. In the past, the price breaks start at 3 dongles and go up from there.
If your lab is interested in purchasing a dongle, please email me directly the name of the PI, an account number to charge, and the number of dongles you wish to purchase by March 17th.
Tim Timothy Bushnell, Ph.D.Research Assistant Professor, Pediatrics and OncologyCo-Director, URMC Flow Cytometry
Purdue Cytometry Mail list-Cytometry Education Associates, Inc-Verity Software House, Inc___a non-profit umbrella organization....http:// www.vsh.com/cytometrycourses/
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Wake up people - times are changing - look at all these new small companies trying to stick their noses in "our" field! Wake up people - times are changing - look at all these new small companies trying to stick their noses in "our" field! Wake up people - times are changing - look at all these new small companies trying to stick their noses in "our" field! Purdue Cytometry Mailing List: Re: 2008 NW Regional Cytometry Meeting, March 13 - 15 in Portland ... Technology, IntelliCyt, Miltenyi Biotec, Partec, StemCell Technologies, Tree Star, Union Biometrica, and Verity Software House. ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1946.htm - 6.9KB 74%
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Purdue Cytometry Mailing List: RE: DNA analysis software ... Purdue Cytometry Mailing List: RE: DNA analysis software ... J. Herbert Technical Support Manager Verity Software House ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0747.htm - 7.7KB 78% |||||||||||||||||||| 26 May 07 Find Similar Highlight Purdue Cytometry Mailing List: Re: DNA analysis software ... vsh.com] > Sent: Wednesday, February 21, 2007 10:30 AM >
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Document count: purdue (139318) cytometry (25468) mail (53709) list (63448) 2007 (37553) verity (222) software (15216) sales (14402) 07 (21574) by (129519) thread (26375) purdue cytometry mail list 2007 verity software sales 07 by... (27962) about 187244 results found, top 500 sorted by relevance score using date hide summaries group by location spacer 1-10 next
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26 May 07
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Mitch Haynes - 27 Aug 2008 04:49 GMT > ISAC XXII International Congress & Purdue Cytometry University ML & > XML [quoted text clipped - 1296 lines] > > - Show quoted text - flowcytometry - 09 Dec 2008 07:31 GMT Re: G3s G4s, MacOS and CellQuest - a cautionary tale • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ Maybe in reply to ] [ Next in thread ] [ Replies ] From: Maciej Simm <simmmmer@yahoo.com> Date: Wed Jan 12 2000 - 23:25:13 EST I had a chat with someone @ BD and it sounds like MacOS X might fix those bugs.. but no names :)
Maciej
===== `---------------------------------------------`
| Maciej S. Simm | 525 E 68th Street | | Research Technician | Room N-805 | | Cornell Medical Center | Tel. 212.746.3428 | `---------------------------------------------` __________________________________________________ Do You Yahoo!? Talk to your friends online with Yahoo! Messenger. http://im.yahoo.com Received on Thu Jan 13 13:00:42 2000 • This message: [ Message body ] • Next message: Howard Shapiro: "Re: Flow Check Particles" • Previous message: Frederic Preffer: "Re: (no subject)" • Maybe in reply to: David Chambers: "G3s G4s, MacOS and CellQuest - a cautionary tale" • Next in thread: Adrian Smith: "Re: G3s G4s, MacOS and CellQuest - a cautionary tale" • Reply: Adrian Smith: "Re: G3s G4s, MacOS and CellQuest - a cautionary tale"
• Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:00 EST Re: G3s G4s, MacOS and CellQuest - a cautionary tale • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] From: Adrian Smith <A.Smith@centenary.usyd.edu.AU> Date: Thu Jan 13 2000 - 20:35:58 EST
>I had a chat with someone @ BD and it sounds like MacOS X might fix >those bugs.. >but no names :) > >Maciej What I don't understand is why BD doesn't put some effort into getting it to work properly with OS9.0 before they even think about OS X. If it works under OS9.0 it will work under OS X (at least that is what has been promised), even if it doesn't take full advantage of the new features of OS X. Besides OS X is still a year away and OS 9 has been shipping for months (and as David originally posted it is the only choice on some machines).
Adrian Received on Fri Jan 14 11:30:48 2000 • This message: [ Message body ] • Next message: Jose A. Stoute: "Problem with Three Color Reagents" • Previous message: Marty Bigos: "Re: G4's will run OS8.6" • In reply to: Maciej Simm: "Re: G3s G4s, MacOS and CellQuest - a cautionary tale" • Next in thread: Keith Bahjat: "G4's will run OS8.6" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:00 EST RE: G3s G4s, MacOS and CellQuest - a cautionary tale • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ Maybe in reply to ] [ Next in thread ] [ Replies ] From: Newsom, Brian S. <BSNEWSOM@txccc.org> Date: Thu Jan 13 2000 - 11:57:04 EST David,
Sorry to hear about your woes. I wanted to pass on the information I have regarding this issue. I have been talking to BD about upgrading some of our FACScans to Macs with Cellquest and have talked to them briefly about G3 vs G4. They know there are problems with OS 9.0 and they are working on them, that is why currently they are only selling G3's. They do have OS 9.0 compatibility in the works and I would expect it would come fairly soon. In our discussions with BD it sounded as though by the time we get our act together to get the necessary machines they may be ready to go with G4's and OS 9.0.
Best of luck, Brian Newsom Director, Flow Cytometry Center for Cell and Gene Therapy Baylor College of Medicine
-----Original Message----- From: David Chambers [mailto:davidc@ccmi.salk.edu] Sent: Tuesday, January 11, 2000 11:26 AM To: cyto-inbox Subject: G3s G4s, MacOS and CellQuest - a cautionary tale
Wotcher Flowers,
I thought I would share my recent experience with everyone.
Recently we had the need to set up a new CellQuest analysis workstation to cope with demand for Mac time. So we ordered a new computer from Apple. It was a G4 machine because that's all they sell now unless you want an iMac or powerbook. Of course, it shipped with MacOS 9.0 installed, and arrived last week. A very nice looking machine, rather reminiscent of a Porsche :-)
B-D now ships CellQuest with a USB dongle, so there were no problems with USB-ADB adapters or the like, and CellQuest installation went fine until we tried to run it. Firstly the BDPAC control panel would not work because the computer told us "BDPAC cannot load because BDPAC cannot be found". Secondly, CellQuest ran perfectly for 5 seconds (approximately) and then the Finder decided to intervene, saying it wanted attention. If we gave it such, it shut down CellQuest saying that the server containing it could no longer be found, then complaining about a Type 3 error. Note that while the finder could not get our attention, CellQuest ran nicely - we could load and save files, analyze, print, etc., but one accidental click outside the CQ window and WHAMMO!
We tried the usual things - increasing CQ memory, turning all unnecessary extensions off, disconnecting the network, etc, etc, all to no avail. "No problem", I thought, "Let's install OS 8.6, we know CellQuest is fine with that"
Except.... THE NEW G4's WILL ONLY RUN MacOS 9.0 or LATER. It is totally impossible to install 8.6 on them. We called Apple for help and were told that this is indeed the case and they couldn't/wouldn't help, hard luck, it was the Application's fault. Apple has changed to a new motherboard in recent machines and it is impossible to use older versions of MacOS. So we gave up and sent the G4 back. We found a blue G3 machine at MacWarehouse, it runs 8.6 and runs CellQuest impeccably.
All the above diatribe is just to warn others thinking of getting a new computer that they may have no prospects of being able to run CellQuest on it. It is sad that Apple should no longer manufacture machines capable of running a previous OS, particularly since OS 9.0 appears to have issues with many other applications software as well.
Pity.
-- David
• application/ms-tnef attachment: stored Received on Fri Jan 14 14:23:29 2000 • This message: [ Message body ] • Next message: Mario Roederer: "Re: CD4 loss with activation" • Previous message: BaltodanoM: "RESEARCH ASSOCIATE II" • Maybe in reply to: David Chambers: "G3s G4s, MacOS and CellQuest - a cautionary tale" • Next in thread: Carl-Magnus Hogerkorp: "RE: G3s G4s, MacOS and CellQuest - a cautionary tale" • Reply: Carl-Magnus Hogerkorp: "RE: G3s G4s, MacOS and CellQuest - a cautionary tale" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:00 EST
Re: G4's will run OS8.6 • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] From: Derek Davies <daviesd2@icrf.icnet.uk> Date: Sun Jan 16 2000 - 16:21:08 EST Hi Keith and others,
Just a few thoughts....
I don't know whether anyone else has the same 'problem', but I am still running my Vantage under System 7.1, chiefly because it does what it is supposed to do ie control the sort and I am unwilling to upgrade and possibly introduce all sorts of problems (which is what happened when I upgraded a previous working HP system to a then new Mac system) - if it ain't broke etc.
This has caused the computer people here (God bless 'em) to tear their hair out and repeatedly ask me to upgrade even if only to 8.1.
I too have been wondering how BD in particular will be dealing with the major changes that will occur with the more UNIX-like feel of OS X (OS 9 does
appear to be a sort of a stopgap). The newer G4 processors are much speedier but we could also take the view that we are being forced into buying hugely overspecced computers by market forces and, of course, software
follows suit - look at the bloated nature of most web browsers (with the honourable exception of iCab) and word processing software. However, it will be incumbent on machine manufacturers to keep up. If I may be Satan's pal for a moment, maybe it would be an idea to consider separate program versions for acquisition and analysis. After all, even today's processors are speedy enough to analyse huge numbers of cells per second and we could keep many machines going on "outdated" hardware for years. Then an analysis program could be freed to keep up with OS changes without the need to make sure that it will also acquire data, speak to the cytometer etc etc. Of course a third party program (?like FlowJo) would do just as well and already exist.
At the moment there seems to be confusion as to which OS will run CellQuest, whether it will work for analysis only etc. Still, could be worse, we might be running Windows and we do have Jonathon Ive's top designs!
Derek
On Thu, 13 Jan 2000, Keith Bahjat wrote: <Snip fully deserved Apple eulogy>
> In the meantime, I would drop a line to BD, reminding them that OS X is > coming in August, at which point their software will not be compatible with [quoted text clipped - 3 lines] > DOS (yuk!). Maybe Verity or TreeStar will make some inroads if we demand > state of the art software. ************************************************************************ Derek Davies Voice: (44) 0207 269 3394 FACS Laboratory, FAX: (44) 0207 269 3100 Imperial Cancer Research Fund, e_mail: derek.davies@icrf.icnet.uk London, UK
Web Page: http://www.icnet.uk/axp/facs/davies/index.html
In tenebris lux ************************************************************************* Received on Tue Jan 18 13:47:20 2000 • This message: [ Message body ] • Next message: Witherspoon, Sam: "Research Triangle Cytometry Association 01- Feb-2000" • Previous message: Michael Ormerod: "Reading Cellquest files" • In reply to: Keith Bahjat: "G4's will run OS8.6" • Next in thread: Newsom, Brian S.: "RE: G3s G4s, MacOS and CellQuest - a cautionary tale" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:00 EST
RE: G3s G4s, MacOS and CellQuest - a cautionary tale • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] [ Replies ] From: Carl-Magnus Hogerkorp <carl-magnus.hogerkorp@molmed.lu.se> Date: Wed Feb 02 2000 - 09:46:40 EST Dear All,
I wonder who to curse (BD,Verity or Apple). Having looked through all inputs on this line of subject, I thought let's just use the WinList on the G4 in the meantime. However, despite the use of iMate the computer can't find the dongle and therefore won't cooperate.
OK that CellQuest doesn't work in the OS9 environment, what about Winlist is it just the ADB connects that is the problem or is it the OS9 again?
What is possible to run on the G4? Shall I send it back?
Carl-Magnus Högerkorp Stem Cell Laboratory University Hospital of Lund Sweden
-----Original Message----- From: Newsom, Brian S. [mailto:BSNEWSOM@txccc.org] Sent: den 13 januari 2000 17:57 To: Cytometry Mailing List Subject: RE: G3s G4s, MacOS and CellQuest - a cautionary tale
David,
Sorry to hear about your woes. I wanted to pass on the information I have regarding this issue. I have been talking to BD about upgrading some of our FACScans to Macs with Cellquest and have talked to them briefly about G3 vs G4. They know there are problems with OS 9.0 and they are working on them, that is why currently they are only selling G3's. They do have OS 9.0 compatibility in the works and I would expect it would come fairly soon. In our discussions with BD it sounded as though by the time we get our act together to get the necessary machines they may be ready to go with G4's and OS 9.0.
Best of luck, Brian Newsom Director, Flow Cytometry Center for Cell and Gene Therapy Baylor College of Medicine
-----Original Message----- From: David Chambers [mailto:davidc@ccmi.salk.edu] Sent: Tuesday, January 11, 2000 11:26 AM To: cyto-inbox Subject: G3s G4s, MacOS and CellQuest - a cautionary tale
Wotcher Flowers,
I thought I would share my recent experience with everyone.
Recently we had the need to set up a new CellQuest analysis workstation to cope with demand for Mac time. So we ordered a new computer from Apple. It was a G4 machine because that's all they sell now unless you want an iMac or powerbook. Of course, it shipped with MacOS 9.0 installed, and arrived last week. A very nice looking machine, rather reminiscent of a Porsche :-)
B-D now ships CellQuest with a USB dongle, so there were no problems with USB-ADB adapters or the like, and CellQuest installation went fine until we tried to run it. Firstly the BDPAC control panel would not work because the computer told us "BDPAC cannot load because BDPAC cannot be found". Secondly, CellQuest ran perfectly for 5 seconds (approximately) and then the Finder decided to intervene, saying it wanted attention. If we gave it such, it shut down CellQuest saying that the server containing it could no longer be found, then complaining about a Type 3 error. Note that while the finder could not get our attention, CellQuest ran nicely - we could load and save files, analyze, print, etc., but one accidental click outside the CQ window and WHAMMO!
We tried the usual things - increasing CQ memory, turning all unnecessary extensions off, disconnecting the network, etc, etc, all to no avail. "No problem", I thought, "Let's install OS 8.6, we know CellQuest is fine with that"
Except.... THE NEW G4's WILL ONLY RUN MacOS 9.0 or LATER. It is totally impossible to install 8.6 on them. We called Apple for help and were told that this is indeed the case and they couldn't/wouldn't help, hard luck, it was the Application's fault. Apple has changed to a new motherboard in recent machines and it is impossible to use older versions of MacOS. So we gave up and sent the G4 back. We found a blue G3 machine at MacWarehouse, it runs 8.6 and runs CellQuest impeccably.
All the above diatribe is just to warn others thinking of getting a new computer that they may have no prospects of being able to run CellQuest on it. It is sad that Apple should no longer manufacture machines capable of running a previous OS, particularly since OS 9.0 appears to have issues with many other applications software as well.
Pity.
-- David
• application/ms-tnef attachment: winmail.dat Received on Wed Feb 2 14:12:01 2000 • This message: [ Message body ] • Next message: Charles Robinson: "Nuclear Pore Antibodies" • Previous message: Dr Sasha Sreckovic: "Re: CloneCyt/Single Cells" • In reply to: Newsom, Brian S.: "RE: G3s G4s, MacOS and CellQuest - a cautionary tale" • Next in thread: Adrian Smith: "RE: G3s G4s, MacOS and CellQuest - a cautionary tale" • Reply: Adrian Smith: "RE: G3s G4s, MacOS and CellQuest - a cautionary tale"
• Reply: Mario Roederer: "RE: G3s G4s, MacOS and CellQuest - a cautionary tale" • Reply: Carl-Magnus Hogerkorp: "RE: G3s G4s, MacOS and CellQuest - a cautionary tale" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:03 EST
RE: G3s G4s, MacOS and CellQuest - a cautionary tale • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] From: Mario Roederer <Roederer@drmr.com> Date: Wed Feb 02 2000 - 16:00:41 EST Carl, don't despair--FlowJo runs fine on G4's in OS9-- you can use it to analyze all of your data files, be they BD, Coulter, Cytomation, or any other fine manufacturer's.
Tree Star will soon have a specific version of FlowJo that will take advantage of the G4 "altivec" processor--allowing significant improvements in calculation speeds by doing multiple simultaneous calculations. In alpha-testing, the altivec-specific version performs compensation 2-5 times faster than the standard version!
For more information, see <http://www.treestar.com/flowjo/>
mr
At 3:46 PM +0100 2/2/00, Carl-Magnus Hogerkorp wrote:
>Dear All, > [quoted text clipped - 99 lines] > >-- David Received on Thu Feb 3 13:57:40 2000 • This message: [ Message body ] • Next message: Sarah Lawson: "Advice/help regarding red cell lysis" • Previous message: Dr Sasha Sreckovic: "Re: Purity??" • In reply to: Carl-Magnus Hogerkorp: "RE: G3s G4s, MacOS and CellQuest - a cautionary tale" • Next in thread: Carl-Magnus Hogerkorp: "RE: G3s G4s, MacOS and CellQuest - a cautionary tale" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:03 EST
Re: Paint-a-Gate • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ Maybe in reply to ] From: Adam Treister <adam@treestar.com> Date: Mon Feb 21 2000 - 18:41:41 EST Helen,
I would presume that Paint-a-Gate will not read Summit's files because BD's software expects 8 or10 bits per data value, and Summit writes 12 bits.
FlowJo has a hidden feature in the Export function that would let you write the files in a format Paint-a-Gate could read (I think). This is not visible in the "consumer" version of the program but I'd can issue you a special serial number to do it. As Larry pointed out, Summit can create these graphs, (as can FlowJo) so doing this translation step may not be worth the trouble.
For more on FlowJo's color gating and overlays, see: <http://www.treestar.com/flowjo/v3/html/leoverlays.html>
Adam
> At 02:31 PM 2/16/00 -0600, Helen Horton wrote: >A very quick question: Does anyone know if data acquired by Summit >software (Cytomation) can be analyzed by Paint-a-Gate software (BD)? >Neither company could answer this for me! ----->>> On 21-Feb-2000, Larry Arnold wrote:
> If you have acquired data with Summit why not use Summit's > color gating feature which is equivalent to Paint-a-Gate? --------------
---------------------------------- Adam Treister Tree Star, Inc. ph: 650-508-9349 fax: 650-508-9186 www.treestar.com/flowjo ---------------------------------- Received on Tue Feb 22 12:14:04 2000 • This message: [ Message body ] • Next message: tessa kerre: "thanks and CB phenotying" • Previous message: Cheryl Smith: "dilution of antibody" • Maybe in reply to: Helen Horton: "Paint-a-Gate" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:06 EST
Re: OS9+Cellquest • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] [ Replies ] From: Adrian Smith <A.Smith@centenary.usyd.edu.AU> Date: Wed Mar 08 2000 - 19:40:50 EST
>Cell Quest runs on G4s running OS9. You just need to purchase a USB to ADB >adapter to attach your dongle. I recommend the I-Mate from Griffin >Technologies. Then download the recent version of the driver from Griffin's >website (http://www.griffintechnology.com/imac/imate_driver.html), which >will support the dongle. Well it doesn't work in the lab next door with a USB dongle and it certainly didn't work for David Chambers (see his post to this list on 13.1.00).
Next door haven't tried it with a USB-ADB adaptor which makes me wonder if the USB dongle might be part of the problem with Mac OS 9.0
We are still using it on a PowerMac 7300/180 for very occasional work. Any heavy duty analysis is done in FlowJo.
Adrian
-- ****************************************************** Adrian Smith (PhD Student) T CELL BIOLOGY GROUP Centenary Institute of Cancer Medicine & Cell Biology Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA. ****************************************************** Received on Thu Mar 9 10:25:20 2000 • This message: [ Message body ] • Next message: Joost Schuitemaker: "RE: Replies to list." • Previous message: Joseph Webster: "Re: Replies to list." • In reply to: Paul Weiss: "Re: OS9+Cellquest" • Next in thread: Paul Weiss: "Re: OS9+Cellquest" • Reply: Paul Weiss: "Re: OS9+Cellquest" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:09 EST
FlowJo and CellQuest (was RE: OS9+Cellquest) • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] From: Adrian Smith <A.Smith@centenary.usyd.edu.AU> Date: Wed Mar 08 2000 - 20:33:48 EST
>OK Flow JO is great ANALYSIS software, for most things....but it still will >not Acquire data, I'm not really sure that is the point here. Haven't we been talking about ANALYSIS?
Most, if not all, the people who have been having problems have bought new G4 machines for post-acqusition analysis (and other uses). Those who simply upgraded to OS 9.0 could easily downgrade to OS8.6 when it didn't work - it was those people with machines that only run OS9.0 that were stuffed (by Apple and by BD)
If you bought one for acquisition then I agree wholeheartedly that you should consider it an object lesson (esp given *BDs* record (AND their recommendations) as well as Apple's)
> allow back gating of populations Excuse me?
>and is not the easiest >for "Graphical" Analysis... That is entirely subjective (and I'm not even sure what you mean by "graphical" analysis!). As I have said before I find that complete novices pick up FlowJo MUCH more quickly than CellQuest.
>so the mantra of "dumping" Cellquest and moving >to FlowJo is over simplistic at best and is only a solution for those who >never touch the FACS their data comes from in the first place. Not true either. I touch the flow cytometer while it collects all my data AND I always use FlowJo for my analysis. No, FlowJo is not a solution for acquisiton but again I don't think that is what we have been talking about. FlowJo has been an excellent solution for us and we do all our own acqusition (using CellQuest on an older machine with an older OS - as you said, if it ain't broke...).
Our data is of such a nature that ANY increase in machine speed directly translates to increased analysis throughput - FlowJo has allowed us to take advantage of those increases in speed. Analysis takes MUCH longer the acqusition in our experiments and acqusition doesn't require any where near the same computing power.
We were even using FlowJo while the cytometer was still running from an HP - CellQuest nowhere in the loop!
I think acquisition and analysis are two separate tasks and it makes sense to use the best tools for each one.
Most people need two computers, and hence two software licenses, anyway...
I don't think I was being "simplistic" in advocating dropping CellQuest :) ( but I should have made it clearer I was talking about ANALYSIS only)
>And it still >isn't available for the PC platform, so once again, it is not a cure-all. Neither is CellQuest last time I looked! Received on Thu Mar 9 11:25:22 2000 • This message: [ Message body ] • Next message: Keith Bahjat: "OS9 and CellQuest: The final chapter??" • Previous message: Joost Schuitemaker: "RE: Replies to list." • In reply to: CJett: "RE: OS9+Cellquest" • Next in thread: Ray Hicks: "RE: OS9+Cellquest" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:09 EST
G4 and OS8.6 • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] From: <David.McFarland@mcmail.vanderbilt.edu> Date: Wed Mar 08 2000 - 10:38:13 EST OK. Imade the mistake of passing along some (mis)information from our computer "expert". I have received numerous responses from this forum that say it is indeed possible to install an OS pre-OS9 on a G4. I'd say contact Apple to get the real lowdown. Sorry if I lead anyone astray.
David McFarland Howard Hughes Medical Institute Flow Cytometry Facility Vanderbilt University Medical Center Received on Wed Mar 8 18:10:16 2000 • This message: [ Message body ] • Next message: Michael_Cosgrove@BDIS.Com: "CellQuest and OS9" • Previous message: Paul Weiss: "Re: OS9+Cellquest" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:09 EST
CellQuest and OS9 and new G4s - Resolved! • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ Next in thread ] [ Replies ] From: David Chambers <davidc@ccmi.salk.edu> Date: Thu Mar 16 2000 - 14:47:08 EST I thought I would just report to the list that BD has done a really great job on making the above combination work correctly. Kudos to them. We received the new version of CellQuest this morning and it works just perfectly!
-- David Received on Wed Mar 22 11:11:40 2000 • This message: [ Message body ] • Next message: Bessent, Eileen F.: "JOB OPPORTUNITY" • Previous message: Julie Oughton: "Re: viability on fixed cells" • Next in thread: David Coder: "Flow Cytometry position in the Pacific Northwest--kayak to work." • Reply: David Coder: "Flow Cytometry position in the Pacific Northwest-- kayak to work." • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:10 EST
Re: Applescript and Flowjo? • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ Maybe in reply to ] From: Adam Treister <adam@treestar.com> Date: Thu Apr 06 2000 - 19:19:50 EST ----->>> On 6-Apr-2000, Jan Hendrikx wrote:
> Has anybody succeeded in scripting Flowjo using Applescript? > It would be great if that were possible!! -------------- I know I haven't.
As Adrian noted, FlowJo does support some minimal level of AppleScript, but doesn't have the object model implementation which is what you'd need to do anything meaningful. I did this in an earlier application (FlexiTrace) and it turned into a huge amount of work to enable obtuse functionality to a small number of users. My personal experience with AppleScripts is that they're really brittle. If you move something, or want to something different from the script writers intention, the script gets annoyingly confused and its much easier to start over than to fix it. Publish and Subscribe is an easier addition feature we've considered, but there just aren't many people who'd take advantage of this if it were included. (Let me know if you think otherwise.)
If properly constructed, a workspace in FlowJo is the script of all analyses and reports in that experiment. Dropping your new data folder onto the workspace should perform all the analyses that one might automate with AppleScript. We have considered automating it further, but the logical next step is auto-printing. In deference to trees, we chose to stop short of automating the output without making you look at the data.
Someday soon, FlowJo will store its workspace in an XML syntax, which means that we can utililze generic scripting tools, or you can even hack the files yourself. I think this is a better approach than addiing AppleScript.
Adam
---------------------------------- Adam Treister Tree Star, Inc. ph: 650-508-9349 fax: 650-508-9186 www.treestar.com ---------------------------------- Received on Fri Apr 7 12:51:48 2000 • This message: [ Message body ] • Next message: Donald Evans: "Postdoctoral position" • Previous message: Christina McCowan: "Re: elastase in neurtophils" • Maybe in reply to: Jan Hendrikx: "Applescript and Flowjo?" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:14 EST
FlowJo/G4/OS X update [was: Cellquest/G4/OS9 update] • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ In reply to ] From: Adam <adam@treestar.com> Date: Thu Apr 27 2000 - 23:53:34 EST bunny wrote:
> And Adam- as soon as FlowJo does histogram overlays (the only thing I > use CQ for these days)- I'm there! FlowJo does histogram overlays. See <http://www.treestar.com/flowjo/v3/html/leoverlays.html>
I called Bunny, and she was referring to the Kolmogorov-Smirnoff statisitic to perform histogram comparison. FlowJo will create and export the Cumulative Distribution Functions (the integral of the histogram) which is the basis of the K/S statistic, so you can easily perform this test with the help of a spreadsheet. But we still don't have that function built in. I have a prototype built, and you'll likely see this feature included one day soon.
We'll probably get it into the OS X compatible release, due when Apple releases the new operating system in the summer. But the tools we need don't get released until next month, so its hard to predict the schedule. Nevertheless, FlowJo worked with OS9 and the G4 the day they shipped, and we'll endeavor to support OS X on that same timeframe.
> BTW- CellQuest FLIES on a G4! Of course, that's relative to CellQuest on a G3. On the same equipment with the same data, FlowJo outperforms CellQuest hands down.
Adam
----------------------------------- Adam Treister Tree Star, Inc. www.flowjo.com 800-366-6045 ----------------------------------- Received on Fri Apr 28 14:17:53 2000 • This message: [ Message body ] • Next message: Mark A. KuKuruga: "Re: BrdU/PI cell cycle analysis" • Previous message: Steve Woodard: "Viability assay using flow cytometry" • In reply to: bunny: "Cellquest/G4/OS9 update" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:17 EST
Interest in a FlowJo Users Meeting at ISAC? • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] From: Adam Treister <adam@treestar.com> Date: Wed May 10 2000 - 18:44:43 EST Dear ISAC Attendees:
I've received a few requests to hold a FlowJo Users Group meeting at the ISAC conference. If this is something you'd be interested in, please drop me a note. If I get sufficient response then I'll arrange a time and place to meet. Of course, you're also welcome to bring your questions (and bring some data!) to our booth (#105) for one-on-one assistance.
Tree Star is sponsoring an "Internet Lounge" at the conference, providing access to email and the web so that you can keep in touch. We will provide several computers, as well as hookups for your laptops. If you have special software or computing needs, please let me know and we'll try to accommodate you.
I look forward to seeing you all in Montpellier,
Adam
---------------------------------- Adam Treister Tree Star, Inc. ph: 650-508-9349 fax: 650-508-9186 www.treestar.com ---------------------------------- Received on Thu May 11 11:30:51 2000 • This message: [ Message body ] • Next message: Joanne Thomas: "Re: TruCount substitute" • Previous message: Boros András dr.: "quest on cortical culture FCM" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:18 EST
G4 for FACSCalibur • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ Next in thread ] [ Replies ] From: Jeannine Navratil <jsn9@imap.pitt.edu> Date: Tue Sep 19 2000 - 16:14:01 EST Hello all!
We want to upgrade our FACSCalibur computer from a Power PC to a G4. I wanted to play it safe and purchase it through BD, but the powers that be think that their price is ridiculously inflated, when we could buy a G4 ourselves for considerably less money. Has anyone done this, and if so, did you run into problems?
My concerns are 1. Installing the card that connects to the cytometer, 2. Making sure that the operating system is compatible with the cytometer and CellQuest software, 3. What does this do to our service contract coverage, since now the computer is no longer covered?
Any advice would be much appreciated!
Jeannine Navratil University of Pittsburgh Arthritis Institute Received on Wed Sep 20 11:28:27 2000 • This message: [ Message body ] • Next message: Isabell Schmitt: "sterile sorting" • Previous message: Fareed Al-Gurg: "Re: Lysing solution" • Next in thread: Matthias Haury: "Re: G4 for FACSCalibur" • Reply: Matthias Haury: "Re: G4 for FACSCalibur" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:36 EST
G4 for FACSCalibur • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ Next in thread ] [ Replies ] From: Jeannine Navratil <jsn9@imap.pitt.edu> Date: Wed Sep 20 2000 - 17:55:54 EST Hello all again!
Well, I've learned a bit since I posted the message yesterday. I spoke with technical support at BD, and according to them, the electronics card that is in our Power PC (7600/120) is compatible with a G4: it just needs to be moved to the PCI slot in the new computer. As far as compatibility, any G4 that does NOT have a dual processor has been tested and is compatible with CellQuest 3.3. Since the dual processor G4s are the latest hot thing, the old G4s are now being sold for bargain prices at places like MacMall or MacConnection.
As far as the service contract goes, buying and connecting up our own computer does not affect our instrument support, but the new computer is not covered under that contract.
The bottom line is, you can do this yourself for considerably less than what BD would like you to pay. I'll keep everyone posted as to how we make out with this.
Sincerely,
Jeannine Navratil University of Pittsburgh Received on Thu Sep 21 13:58:34 2000 • This message: [ Message body ] • Next message: Mark A. KuKuruga: "Re: sterile sorting" • Previous message: Lucille H Kimura: "Unusual Case" • Next in thread: Ray Lannigan: "Re: G4 for FACSCalibur" • Maybe reply: Ray Lannigan: "Re: G4 for FACSCalibur" • Maybe reply: D. Robert Sutherland: "Re: G4 for FACSCalibur" • Maybe reply: David Chambers: "Re: G4 for FACSCalibur" • Maybe reply: David Chambers: "Re: G4 for FACSCalibur" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:36 EST
Re: G4 for FACSCalibur • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ In reply to ] From: Matthias Haury <mhaury@igc.gulbenkian.pt> Date: Thu Sep 21 2000 - 05:12:47 EST Hey y'all,
I have upgraded our Calibur to a G4, it's easy if you already have a PCI BDIO Board (from 7600 PPC or G3), if you have the Quadra, you will need to purchase another PCI board from BDIS, which I guess is difficult to buy separately...
Also if you do so, you should buy an i-Mate USB-ADB converter to connect the ADB Dongle to the USB Bus (or have a new USB dongle from BDIS), also make sure that you are not using any SCSI Devices for backup, or order a separate SCSI 2906 Card from Adaptec for the G4.
You also need System 9.04, and BD Inits 3.4 and Cellquest 3.3 (all of which I guess you can get from BDIS as free upgrade ???)
Last not least you have to know that BDIS of course will no longer cover any problems related to the computer or BDIO communication, so if you have a service contract you might loose the protection for all I/O related problems (this you have to discuss with your BDIS rep).
We have no problems because I don't have a service contract, and we have enough competence for all Mac OS related problems...
Besides this it works beautifully...
Hope that clarifies things..
Matthias
_____________________________________ Matthias Haury Instituto Gulbenkian de Ciencia Rua Da Quinta Grande N° 6 - Apt.14 P-2781-901 Oeiras Codex Portugal Email: mhaury@igc.gulbenkian.pt Tel: + 351 21 440 79 85 Office Fax: + 351 21 440 79 70 _____________________________________
> From: Jeannine Navratil <jsn9@imap.pitt.edu> > Organization: University of Pittsburgh [quoted text clipped - 20 lines] > University of Pittsburgh > Arthritis Institute Received on Thu Sep 21 15:43:34 2000 • This message: [ Message body ] • Next message: Tom McHugh: "steric hindrance?" • Previous message: Ress, S, Stan, Dr: "Re: Luminex technology" • In reply to: Jeannine Navratil: "G4 for FACSCalibur" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:36 EST FlowJo Training in Boston Sept 26 • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] From: Adam Treister <adam@treestar.com> Date: Wed Sep 20 2000 - 14:02:17 EST It is gratifying to see a growing demand we're getting for training in how to use FlowJo. There are more and more people hearing about the program, realizing that it can help them in their analysis, and wanting to learn its novel approach to experiment based analysis.
I will be giving two training sessions in using FlowJo software in the Longwood area of Boston on Tuesday, Sept 26. There is a morning session from 11:30 am - 1:00pm at Dana Farber Cancer Institute, in Dana 5 Conference Room (Rm D521) and an afternoon session from 2:00 to 3:30 in the Level 6 Conference Room at the Harvard Institute of Medicine Building.
Both sessions are open to the general flow community.
------------------------------------------------------------ Adam Treister Tree Star, Inc. ph: 1-650-508-9349 fax: 1-650-508-9186 adam@treestar.com <http://www.treestar.com> ------------------------------------------------------------ Received on Thu Sep 21 11:43:34 2000 • This message: [ Message body ] • Next message: James, Cathy: "Position Available" • Previous message: gerhard nebe-von-caron: "RE: labelling of Chlamydia bacteria" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:36 EST
Re: G4 for FACSCalibur • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ Maybe in reply to ] [ Next in thread ] From: Ray Lannigan <lannigan@tritechinc.com> Date: Thu Sep 21 2000 - 08:54:56 EST Jeannine, The cytometer interface card that is inside your Power PC can be put into a G4. The installation is simple enough to be done by yourself or your in house computer people. If neither of those options are feasible, give me a call I can help you do it. It is my understanding that when upgrading to OS9 or greater from OS8.6 or lower, there is an incompatibility issue with the BD inits.There was a e-mail posted by a BD rep.on March 8, 2000 that addresses the incompatibility issue. You can refer to this e-mail through the archives, or I can forward you a copy if you prefer. As far as your service contract; Tritech can provide you with a service contract for your FacsCaliber with the new G4 for a price that is considerably lower than BD's. If you would like more information please contact me at one of the numbers listed below. Regards, Raymond Lannigan Tritech Inc. 2014 Renard Ct. Suite I Annapolis, MD 21401 1-410-266-1522 1-800-886-7004 www.tritechinc.com -----Original Message----- From: Jeannine Navratil <jsn9@imap.pitt.edu> To: cyto-inbox Date: Wednesday, September 20, 2000 5:53 PM Subject: G4 for FACSCalibur
>Hello all! > [quoted text clipped - 14 lines] >University of Pittsburgh >Arthritis Institute Received on Thu Sep 21 14:28:38 2000 • This message: [ Message body ] • Next message: Andrea Illingworth: "microscopic image transmission" • Previous message: Mark A. KuKuruga: "Re: sterile sorting" • Maybe in reply to: Jeannine Navratil: "G4 for FACSCalibur" • Next in thread: D. Robert Sutherland: "Re: G4 for FACSCalibur" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:36 EST Re: G4 for FACSCalibur • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ Maybe in reply to ] [ Next in thread ] [ Replies ] From: D. Robert Sutherland <rob.sutherland@utoronto.ca> Date: Fri Sep 22 2000 - 12:55:50 EST There are several cheaper ways to upgrade non-G3, Power PC-based CPUs on your FACStation, without buying a new G4 or dual processor G4.
As pointed out by other correspondents, if you buy a G4, you will probably have to deal with a variety of 'connectivity issues' such as the need for USB connectable printers/adaptors, dongles and any other SCSI-based devices currently in use such as Zip drives etc. Although these issues can be accommodated (at a cost) as Matthias et al have suggested, dealing with such problems has caused major headaches for a number of users tempted to upgrade to G4s. Furthermore, to my knowledge at least, BD have not upgraded Cellquest to take advantage of the Altivec processing capability of the G4 chip. Therefore, Cellquest will not run significantly faster on a G4, than on a G3 rated at the same processor clock speed. Similarly, Cellquest will not run any faster on a dual processor G4 running OS 9.x. While the upcoming Mac OSX will allow many more applications to take advantage of the dual G4s, a major re-work of Cellquest will most likely be required for OSX compatibility. Are BD even planning such an upgrade, and if so, when?
Instead, for about US$200.00, you can purchase a G3 upgrade card (running at about 400Mz with a 1meg backside L2 cache) for your 7300/7500/7600 Power PC Mac and make a huge gain in performance. Such cards are incredibly easy to install by swapping out the old PPC604e card found in all PCI-based Macs. Even faster upgrades are available and you can even get G4 upgrade cards for these Macs if you really want one. However, unless you are planning to use your FACStation computer to run Altivec enhanced applications such as the recent version of Adobe Photoshop et al, I see little reason to go through all the connectivity hassles (and cost) for little if any gain.
This may not be everyone's idea of fun, but I recently purchased as separate items, a 500Mz ZIF (the 'processor') and a 'PCI Carrier card'. (You can also get them as a single card with the ZIF already 'soldered' into the card). I removed the 266 ZIF from the 'Beige' G3 in my office and replaced it with the 500Mz ZIF. I then installed the 266Mz ZIF into the carrier card and installed it into a 7300 running our dear old FACScan. The 200Mz PPC 604e card that I removed from the 7300 was then installed in a Calibur running off a 7600 containing an even older 120Mz PPC 604. Thus, we managed to upgrade 3 Macs for the price of one, we have not had to deal with connectivity issues with any of them, and all we have left over is one old PPC 604 card to use as a door stop.
My 2c worth.
Rob Sutherland University Health Network University of Toronto Received on Mon Sep 25 13:58:57 2000 • This message: [ Message body ] • Next message: Khalid Al-Hussein: "Postion Open" • Previous message: Houston, Jim : "RE: steric hindrance?" • Maybe in reply to: Jeannine Navratil: "G4 for FACSCalibur" • Next in thread: Keith Bahjat: "Re: G4 for FACSCalibur" • Reply: Keith Bahjat: "Re: G4 for FACSCalibur" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:37 EST
FlowJo v3.2 adds new Histogram Comparisons • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] From: Adam Treister <adam@treestar.com> Date: Sun Jul 23 2000 - 01:15:45 EST Dear Flow-ers,
We have released a new version of FlowJo, available for immediate download. Along with sundry bug fixes and refinements, this version contains a powerful new algorithm for comparing histograms.
Version 3.2 of this popular off-line analysis program provides a unique integrated platform for comparing univariate distributions. FlowJo provides the standard K-S statistic, with its inherent limitations, but we also provide a new metric to compare histograms. This "Chi-Squared T Value" provides an indication of the statistical significance with which two histograms are different, and its value scales with the degree of difference. This means that, for the first time, histograms can be ranked by a value that conveys how different they are!
We would like to invite everyone to explore the power of this new algorithm. For more information, see <http://www.flowjo.com/v3/html/comparison.html>.
We are maintaining our policy that allows you to download and try FlowJo for 60 days at no charge. See <http://www.flowjo.com> for more information and download instructions.
Adam
---------------------------------- Tree Star, Inc. New Phone Number: 1-650-591-2854 Or Toll Free (in US): 1-800-366-6045 fax: 1-650-508-9186 www.flowjo.com ---------------------------------- Received on Mon Jul 24 11:41:31 2000 • This message: [ Message body ] • Next message: Derek Davies: "Re: Phi Phi Lux dye" • Previous message: Tom Mc Closkey: "problem with Coherent 305 laser- diagnosis?" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:27 EST
Re: Applescript and Flowjo? • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] From: Adrian Smith <A.Smith@centenary.usyd.edu.AU> Date: Thu Apr 06 2000 - 18:04:46 EST
> Dear Flowers, > [quoted text clipped - 5 lines] >Jan >-- There appears to be a small AppleScript dictionary for FlowJo 3.1 but it looks to be just the standard commands, ie nothing specific to FlowJo. Not knowing anything about programming in Apple Script I have no idea if this is of any use but you might be able to do something with them...
What sort of things did you want to do with AppleScript? Personally I can't really thing anything that I do that AppleScript would make easier/quicker, but that may be just because I don't know much about AppleScript.
Depending on what you want to do you might be able to do it with one of the macro programs like OneClick, Quickeys or Key Quencer. A while ago I made a simple OneClick palette to make some FlowJo features more accessible. It saved me a bit of time for a while but I eventually gave up using it because OneClick seemed to decrease FlowJo's stability.
Adrian Received on Fri Apr 7 12:06:47 2000 • This message: [ Message body ] • Next message: R.Wadley: "cell line" • Previous message: David.McFarland@mcmail.vanderbilt.edu: "Cytometry journal contact info" • In reply to: Jan Hendrikx: "Applescript and Flowjo?" • Next in thread: Adam Treister: "Re: Applescript and Flowjo?" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:14 EST
Re: Apple grumble... • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] From: Adrian Smith <A.Smith@centenary.usyd.edu.AU> Date: Mon Aug 07 2000 - 15:32:51 EST At 4:41 PM -0700 4/8/2000, David Chambers wrote:
> > >> Okay, is there any way that I can pursuade Cell Quest version 3.1 to [quoted text clipped - 7 lines] > >-- David I'm pretty sure we have it running for analysis only on our G4/400 with OS9.0.4. We have a Griffin iMate for the dongle (USB to ADB convertor) and I think we just copied it from our old G3 (along with the EVE INIT for the dongle).
I'll confirm when I get into the lab...(I don't actually use it myself)
What exactly have you tried David?
Adrian Smith Received on Tue Aug 8 12:43:32 2000 • This message: [ Message body ] • Next message: Howard Shapiro: "Re: PI and sample tubing" • Previous message: Cary Isaacs: "MoFlo for sale" • In reply to: David Chambers: "Re: Apple grumble..." • Next in thread: Adrian Smith: "Re: Apple grumble..." • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:29 EST
Re: Apple grumble... • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ Maybe in reply to ] From: Adrian Smith <A.Smith@centenary.usyd.edu.AU> Date: Tue Aug 08 2000 - 20:11:22 EST At 12:48 PM -0700 8/8/2000, David Chambers wrote:
> > >> What exactly have you tried David? [quoted text clipped - 8 lines] > >--D Just to confirm that we have no problems doing analysis with CellQuest 3.1f on a G4/400 (AGP graphics) with MacOS 9.0.4. No problems with the Finder at all...
We use a iMate for Griffen to plug the ADB dongle into the the USB port.
We just copied the CellQuest application folder and the EVE INIT (version 1.10) over from our old G3 and every has worked perfectly.
The only problem is that once CellQuest has been run, FlowJo will not recognise its ADB dongle until we restart.
I suspect the 9.0.4 might be an important factor here (the update was released the day we received the machines so we have never tried things with an earlier version of OS9).
Adrian Smith Received on Wed Aug 9 12:43:38 2000 • This message: [ Message body ] • Next message: Michael Egan: "Re: formalin/paraformaldehyde" • Previous message: Joseph Webster: "Microns (and other small confusions)" • Maybe in reply to: zip@biotransplant.com: "Apple grumble..." • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:29 EST
Re: G4 for FACSCalibur • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] [ Replies ] From: Keith Bahjat <kbahjat@ufl.edu> Date: Mon Sep 25 2000 - 18:24:38 EST
> Furthermore, to my knowledge > at least, BD have not upgraded Cellquest to take advantage of the [quoted text clipped - 6 lines] > University Health Network > University of Toronto Actually, BD never upgraded CellQuest to take advantage of the PowerPC (i.e. modified to be a native PowerPC app), much less AltiVec!! I wouldn't hold my breath for the AltiVec enhanced cellquest any time soon...
BUT, if you use other applications on your cytometer's computer, the G4 may not be a bad idea. Apps like Photoshop utilize AltiVec and run incredibly quick on the G4. Nice to have for the future...
The iMate (USB to ADB adapter) is cheap and easy to install, and in my experience gives 0 trouble. I don't know how much of a "hassle" it is to connect an extra adapter to the end of the Cellquest dongle?? Certainly less of a hassle than installing and configuring a G3 upgrade card..
Keith Bahjat kbahjat@ufl.edu Received on Tue Sep 26 13:59:03 2000 • This message: [ Message body ] • Next message: Martin Kelly: "Re: bronchial lavages" • Previous message: Fischer, Randy (NIAMS): "RE: In search of a Multichannel Pippete for 24 well plates." • In reply to: D. Robert Sutherland: "Re: G4 for FACSCalibur" • Next in thread: Mario Roederer: "Re: G4 for FACSCalibur" • Reply: Mario Roederer: "Re: G4 for FACSCalibur" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:37 EST Re: G4 for FACSCalibur • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] [ Replies ] From: Mario Roederer <Roederer@drmr.com> Date: Tue Sep 26 2000 - 15:16:30 EST I tested FlowJo for a number of different tasks (e.g.,generating complex graphical layouts; generating large tabular outputs; even analyzing 13-parameter, million-event data files) on G4's vs. G3. The G4 was significantly faster (taking into account the clock speed), even when the program was not G4-optimized. I also tested a G4-optimized version of FlowJo that takes advantage of the AltiVec instructions (no, not yet commercially available!), and it's another large step up.
As more and more applications come out to be AltiVec-aware (and they are!), everyone will realize the enormous speed benefit from this technology.
Note that the multi-processing nature of Altivec should not be confused with the multi-processing capability of dual-processor machines (like the new G4 cube). There's a completely different set of optimizations that programs need to fully take advantage of multi-processor machines (and I'm sure that will be forthcoming in the next year or two as well).
Bottom line--G4's are faster, but G3's are a lot cheaper (right now). But as more and more applications are Altivec-aware, then there won't be a comparison.
mr
(PS--Intel-based machines, even the gigaherz processors, don't come close to the G4 capabilities.) Received on Wed Sep 27 12:44:13 2000 • This message: [ Message body ] • Next message: Nebe, Thomas C.: "AW: interesting clinical case" • Previous message: Anja Porwit: "Re: Intracellular Staining" • In reply to: Keith Bahjat: "Re: G4 for FACSCalibur" • Next in thread: J. Paul Robinson: "Re: G4 for FACSCalibur - and a dig at Mario!!" • Reply: J. Paul Robinson: "Re: G4 for FACSCalibur - and a dig at Mario!!" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:37 EST
Re: G4 for FACSCalibur - and a dig at Mario!! • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] [ Replies ] From: J. Paul Robinson <jpr@flowcyt.cyto.purdue.edu> Date: Wed Sep 27 2000 - 20:23:51 EST So Mario, when are you going to talk about software that runs on computers that the other 90% plus that people have.......in terms of world computer sales, Apple is right up there at .....4%!! Paul
Date sent: Tue, 26 Sep 2000 16:16:30 -0400 To: cyto-inbox From: Mario Roederer <Roederer@drmr.com> Subject: Re: G4 for FACSCalibur
> I tested FlowJo for a number of different tasks (e.g.,generating > complex graphical layouts; generating large tabular outputs; even [quoted text clipped - 24 lines] > (PS--Intel-based machines, even the gigaherz processors, don't come > close to the G4 capabilities.) J.Paul Robinson, Ph.D., Professor of Immunopharmacology Professor of Biomedical Engineering Director, Purdue University Cytometry Laboratories Hansen Hall, Roon B050 Purdue University, West Lafayette, IN 47907-1515 (Ph) (765) 494-0757 Fax (765) 494-0517 jpr@flowcyt.cyto.purdue.edu
http://www.cyto.purdue.edu
http://www.bioscope.org Received on Thu Sep 28 13:55:27 2000 • This message: [ Message body ] • Next message: Thanos P Kakoulidis: "job opportunity" • Previous message: Dorte Christiansen: "Expression of Tissue Factor on monocytes" • In reply to: Mario Roederer: "Re: G4 for FACSCalibur" • Next in thread: Karim Vermaelen: "Re: G4 for FACSCalibur" • Reply: Karim Vermaelen: "Re: G4 for FACSCalibur" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:37 EST
RE: G4 for FACSCalibur - and a plug for Mario!! • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ Maybe in reply to ] [ Next in thread ] [ Replies ] From: Fischer, Randy (NIAMS) <fischer1@mail.nih.gov> Date: Thu Sep 28 2000 - 16:59:18 EST Paul,
Like you and many others in this world, I have one of the other 90% of the computers at home. I do not like it, but the other major occupant of the household (my wife) required it for her job. However, like 90% of the Folw Cytometry users out there, I have a BD instrument that uses a MAC to acquire and analyze my data. So, I subscribe to the "do work at work" theory and use the MAC, which by the way I do prefer. Mario's (and Adam's) FlowJo software is a very nice alternative, and any software that is an alternative to paying big bucks to certain Flow companies should be most heartily encouraged.
So, let Mario plug away and let the users of the minority instruments tout their advantages if they wish as well. That is, if there are any???
Randy Fischer NIAMS/NIH
> ---------- > From: J. Paul Robinson [quoted text clipped - 56 lines] > > http://www.bioscope.org Received on Fri Sep 29 14:39:45 2000 • This message: [ Message body ] • Next message: Karim Vermaelen: "Re: G4 for FACSCalibur" • Previous message: Eric Van Buren: "Re: Lasers wet or dry" • Maybe in reply to: Reed, Doug S Dr USAMRIID: "G4 for FACSCalibur - and a plug for Mario!!" • Next in thread: Adrian Smith: "RE: G4 for FACSCalibur - and a plug for Mario!!" • Reply: Adrian Smith: "RE: G4 for FACSCalibur - and a plug for Mario!!" • Reply: Adrian Smith: "RE: G4 for FACSCalibur - and a plug for Mario!!" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:37 EST
RE: G4 for FACSCalibur - and a plug for Mario!! • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] [ Replies ] From: Adrian Smith <A.Smith@centenary.usyd.edu.AU> Date: Fri Sep 29 2000 - 19:06:31 EST And even if you are using the "other" platform for acqusition, you can always buy a Mac just to run FlowJo. Some of us think it is that good...
Adrian
>Paul, > [quoted text clipped - 28 lines] > > > Received on Tue Oct 3 13:49:44 2000 • This message: [ Message body ] • Next message: Len Brown: "RE: G4 for FACSCalibur - and a plug for Mario!!" • Previous message: Julie Moore: "Re: placental digestion" • In reply to: Fischer, Randy (NIAMS): "RE: G4 for FACSCalibur - and a plug for Mario!!" • Next in thread: James Weaver 301-594-5879 FAX 301-594-3037: "Re[2]: G4 for FACSCalibur" • Reply: James Weaver 301-594-5879 FAX 301-594-3037: "Re[2]: G4 for FACSCalibur" • Reply: J.Paul Robinson: "RE: G4 for FACSCalibur - and a plug for Mario!! and one more comment.." • Reply: James Weaver 301-594-5879 FAX 301-594-3037: "Re[2]: G4 for FACSCalibur" • Reply: J.Paul Robinson: "RE: G4 for FACSCalibur - and a plug for Mario!! and one more comment.." • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:38 EST RE: G4 for FACSCalibur - and a plug for Mario!! • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ Maybe in reply to ] [ Next in thread ] From: Len Brown <lbrown@mmri.mater.org.au> Date: Sun Oct 01 2000 - 21:03:33 EST Dear Randy
I have no preference when it comes to computers, except like the >90% of computer literates, I was "brought up" on IBMs, DOS and Windows. The Macs ARE extremely powerful (and rather expensive) but I do find it easier to operate a Windows based PC because I am more familiar with them. I now work with BD cytometers but initially worked on Coulters with their "so-called" redundant operating System II software, which I thought was great for the jobs I was doing.
To my point, like many Institutions, we have an in-house computer network which runs on Windows NT. We have invested heavily in PCs and it makes sense to use the network to analyse flow data. The alternative is to purchase additional Macintosh computers, not a cheap option. I transfer data from the MACS to our NT server where all members of the Institute can access their data using Windows software. We currently use FCS Express and users are generally very happy with it.
FlowJo is a great package and if you need the facilities it offers such as software compensation and ratios etc. it is worth having a copy on your Macs. Also, if you need the enhanced processing power of the new G4s then that's the way to go. Otherwise, I don't see there is a great argument either way.
Use the tools that suit the job!
Len Brown Mater Medical Research Institute Brisbane, Australia
-----Original Message----- From: Fischer, Randy (NIAMS) [mailto:fischer1@mail.nih.gov] Sent: Friday, September 29, 2000 7:59 AM To: cyto-inbox Subject: RE: G4 for FACSCalibur - and a plug for Mario!!
Paul,
Like you and many others in this world, I have one of the other 90% of the computers at home. I do not like it, but the other major occupant of the household (my wife) required it for her job. However, like 90% of the Folw Cytometry users out there, I have a BD instrument that uses a MAC to acquire and analyze my data. So, I subscribe to the "do work at work" theory and use the MAC, which by the way I do prefer. Mario's (and Adam's) FlowJo software is a very nice alternative, and any software that is an alternative to paying big bucks to certain Flow companies should be most heartily encouraged.
So, let Mario plug away and let the users of the minority instruments tout their advantages if they wish as well. That is, if there are any???
Randy Fischer NIAMS/NIH
> ---------- > From: J. Paul Robinson [quoted text clipped - 56 lines] > > http://www.bioscope.org Received on Tue Oct 3 14:22:17 2000 • This message: [ Message body ] • Next message: Jaroslav Dolezel: "Re: isolation of plant nuclei" • Previous message: Adrian Smith: "RE: G4 for FACSCalibur - and a plug for Mario!!" • Maybe in reply to: Reed, Doug S Dr USAMRIID: "G4 for FACSCalibur - and a plug for Mario!!" • Next in thread: Len Brown: "RE: G4 for FACSCalibur - and a plug for Mario!! " • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:38 EST
Re: G4 for FACSCalibur • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] From: Karim Vermaelen <Karim.Vermaelen@rug.ac.be> Date: Mon Oct 02 2000 - 07:41:27 EST "J. Paul Robinson" wrote:
> So Mario, when are you going to talk about software that runs on computers that > the other 90% plus that people have.......in terms of world computer > sales, Apple is right up there at .....4%!! > Paul So if FlowJo was a PC-only application I would have to buy a Wintel machine on top of my Mac, but that wouldn't matter because we're a minority anyway? The 4% your talking about are indeed an estimate of worldwide Apple hardware sales, but we're talking education/academic sector here, where the Mac market share rises to 20% (and much higher in the flow cytometry area as we all know) . I' ve worked on both platforms and believe me I wouldn't even think about doing my FACS work (or any other daily lab tasks) on an NT machine. This may not be very obvious for someone with an engineering background, but I just like the fact that I can easily do maintenance and troubleshooting on our Mac network without any expert IT support. So all things taken into consideration I think the choice of developing software such as FlowJo on Macs is a very pragmatic one.
But the real shocking news now is this report by Keith Bahjat stating that Cellquest is not only non-optimized for the G4's vector processing, but was never optimized for the PowerPC architecture as well! What a shame and a waste of power: a 68k-application on a G4!! I hope someone from BD is reading this and can comment on this shortcoming in development.
Karim Vermaelen Pulmonary Immunobiology Ghent University Hospital Ghent, Belgium
> Date sent: Tue, 26 Sep 2000 16:16:30 -0400 > To: cyto-inbox [quoted text clipped - 42 lines] > > http://www.bioscope.org Received on Tue Oct 3 09:52:54 2000 • This message: [ Message body ] • Next message: sandeep ramalingam: "Quantitation of antigens" • Previous message: Fischer, Randy (NIAMS): "RE: G4 for FACSCalibur - and a plug for Mario!!" • In reply to: J. Paul Robinson: "Re: G4 for FACSCalibur - and a dig at Mario! !" • Next in thread: David Chambers: "Re: G4 for FACSCalibur" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:37 EST Re: G4 for FACSCalibur • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ Maybe in reply to ] [ Next in thread ] From: David Chambers <davidc@ccmi.salk.edu> Date: Tue Oct 03 2000 - 11:54:05 EST On Mon, 02 Oct 2000, Karim Vermaelen wrote:
> But the real shocking news now is this report by Keith Bahjat stating that > Cellquest is not only non-optimized for the G4's vector processing, but was never > optimized for the PowerPC architecture as well! I don't speak for B-D, but on the CellQuest box is a large red starry sticker saying "Accelerated for Power Macintosh". Presumably this is not there by accident? Naahhhh... :-)
-- David Received on Tue Oct 3 18:49:46 2000 • This message: [ Message body ] • Next message: James Weaver 301-594-5879 FAX 301-594-3037: "Re[2]: G4 for FACSCalibur" • Previous message: Maciej Simm: "choice of BRIGHT antibodies/fluorochromes for monocytic IL-10 study?" • Maybe in reply to: Jeannine Navratil: "G4 for FACSCalibur" • Next in thread: David Chambers: "Re: G4 for FACSCalibur" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:38 EST
Re[2]: G4 for FACSCalibur • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] [ Replies ] From: James Weaver 301-594-5879 FAX 301-594-3037 <WEAVER@CDER.FDA.GOV> Date: Tue Oct 03 2000 - 14:49:02 EST However since BD is currently developing and testing software on an NT platform for their sorters, this entire discussion will become even more pointless than it is now within the next year or two.
-Jim Weaver Received on Tue Oct 3 19:19:45 2000 • This message: [ Message body ] • Next message: joan Kalnitsky: "permeabilization of feline cells" • Previous message: David Chambers: "Re: G4 for FACSCalibur" • In reply to: Adrian Smith: "RE: G4 for FACSCalibur - and a plug for Mario!! " • Next in thread: Marilyn Dietrich: "Re: BD and windows based platform" • Reply: Marilyn Dietrich: "Re: BD and windows based platform" • Reply: Marilyn Dietrich: "Re: BD and windows based platform" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:38 EST
RE: G4 for FACSCalibur - and a plug for Mario!! and one more comment.. • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] From: J.Paul Robinson <jpr@flowcyt.cyto.purdue.edu> Date: Wed Oct 04 2000 - 12:27:16 EST The issue of platforms actually does become important and more so than if you just like one more than the other. I don't want to bash this issue, but last week the New York Times reported Apple stock fell 58% and things are not so rosy it seems, and today in the Wall Street Journal Apple looks like its going to take another hit..with lost sales and production goals... worlwide sales dropped to 3.2% .
The issue really is important - Coulter once invested heavily in the Terak system becuase it had fantastic graphics (and it did..) it was very expensive and many people invested .. (like us) but I suspect there is not a single one operating today.
B-D appears to be walking a fine line - they went from HP I think to MACs and from what I could gather at the ISAC meeting in May they appear to be pushing a hybrid PC data collection system and a MAC analysis system. It is unclear to me if they are going to PC or not. I suspect that you don't just jump from one platform to the other --- anyway, this is an issue of sorts since in looking to purchase a new system for our lab, I need to appreciate the complicating factors of how to deal with a lab that has 40 odd PCs and a MAC......and the real question for me is not how good the MAC is, but how long it will be around....is the cube another NEXT...???
I like FloJo by the way - think its great software !!!
Paul Robinson On 30 Sep 2000, at 11:06, Adrian Smith wrote:
And even if you are using the "other" platform for acqusition, you can always buy a Mac just to run FlowJo. Some of us think it is that good...
Adrian
>Paul, > [quoted text clipped - 28 lines] > > > J.Paul Robinson, PhD PH:(765)4940757 Professor of Immunopharmacology Professor of Biomedical Engineering Purdue University FAX:(765)4940517 EMAIL:jpr@flowcyt.cyto.purdue.edu WEB: http://www.cyto.purdue.edu Received on Fri Oct 6 13:36:23 2000
• This message: [ Message body ] • Next message: Nathalie Tessier: "[high speed sorting of ES cells" • Previous message: Mark A. KuKuruga: "Re: unstable FACScan readings" • In reply to: Adrian Smith: "RE: G4 for FACSCalibur - and a plug for Mario!! " • Next in thread: Adrian Smith: "RE: G4 for FACSCalibur - and a plug for Mario!!" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:44 EST
Re: BD and windows based platform • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] From: Marilyn Dietrich <mdietrich@mail.vetmed.lsu.edu> Date: Wed Oct 04 2000 - 13:31:39 EST I hope that BD is developing and testing software on a windows 2000 platform since it is replacing NT. If everything works fine with NT it more than likely will work with windows 2000, however, just in case there are problems I still hope they've move on to 2000.
-Marilyn Dietrich
At 03:49 PM 10/3/00 -0400, you wrote:
>However since BD is currently developing and testing software on an NT >platform for their sorters, this entire discussion will become even more >pointless than it is now within the next year or two. > >-Jim Weaver Received on Fri Oct 6 11:50:02 2000 • This message: [ Message body ] • Next message: MARDER_PHILIP@Lilly.Com: "GLIIFCA Golf Scramble" • Previous message: Anja Porwit: "Quantitation ofCD4" • In reply to: James Weaver 301-594-5879 FAX 301-594-3037: "Re[2]: G4 for FACSCalibur" • Next in thread: Marilyn Dietrich: "Re: BD and windows based platform" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:38 EST
BD and Coulter • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] From: Jeffrey M Scott <jeffreys@bcm.tmc.edu> Date: Wed Oct 04 2000 - 16:31:19 EST Randy Fischer wrote "However like 90% of the Flow Cytometry users out there, I have a BD instument" ?!? I was under the impression that the distibution of brands was close to 50/50. Perhaps it varies by geographical region
JMScott Core Facility Baylor College of Medicine Received on Fri Oct 6 10:50:07 2000 • This message: [ Message body ] • Next message: FACS_COPY@wehi.edu.au: "Re: unstable FACScan readings" • Previous message: Carolyn Jefferiss: "FacStar Plus offer 5/10/00" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:44 EST
re: RE: G4 for FACSCalibur - and a plug for Mario!! and one more comment.. • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ Next in thread ] [ Replies ] From: D. Robert Sutherland <rob.sutherland@utoronto.ca> Date: Tue Oct 10 2000 - 01:31:31 EST My, My, I certainly stirred up a hornets nest with my last post, the simple objective of which was to point out how technically undemanding and cheap it is to upgrade 'legacy' Macs such that even those in the 7300/7500/7600 family can be made to go as fast as 'you know what off the proverbial shovel'. And all without any help from or taxes paid (in the form of 'all-new G4 workstations') to BD!!
Since then, we have heard disturbing news from Keith who reckons that BD have not even upgraded Cellquest to PowerPC native code, let alone to be Altivec enhanced (for G4s, or upgraded G4s). Keith also politely (I think) pointed out that is very simple to install a ADB:USB dongle (which it now is). However, I recall when the drivers first came out for this adaptor, a number of users had problems. Of course they have been updated (yet another new one last week) to the point where even a PC user could probably do this without calling the IT department. The point however is that other connectibility issues outlined in my earlier epistle (such as installing SCSI cards, USB Zip drivers etc. serial/AppleTalk printers, floppy drives, Superdrives, etc.) are nowhere near as simple, cheap or idiot-proof as installing a G3 or G4 upgrade card into a fully functional workstation, that in many labs is networked to other cytometers, computers, printers etc (i.e., legacy peripherals).
Next, Mario opined that the G4 was 'significantly faster' even taking into account the clock speed of the processor'. The only explanation for this observation is that other parts of the G4 are responsible for these speed increases. G4s come with more (and faster) RAM, more (and faster) Video Ram, the Bus speed is faster, the Video subsystems are faster (blah blah). However, for non-Altivec enhanced apps, G4s are NOT any faster than G3s at the same clock speed, as orginally stated. There have been hundreds of controlled tests performed (check out xlr8yourmac.com) comparing the same computer with a G3 or a G4 (at the same clock speed) on Altivec enhanced versus non-enhanced apps: The results are always the same, the G4 blows the doors off G3s (and PCs) on Altivec enhanced apps such as Photoshop. On other apps there is NO difference between G4s and G3s!! I hope Mario is correct when he says that more apps are to be made 'Altivec aware', starting with the software that about 90% of BD users require, i.e., Cellquest!! Incidentally Mario, the G4 Cube does not and probably never will contain (for cooling reasons) dual G4 processors.
Thereafter, Paul himself weighed in with estimates of Apple's market share at 4%, (a later post reduced this even further). Regardless of whether these numbers came from the 'Microsoft Book of Facts' (apparently a very thin volume indeed) or other reputable sources, the point is irrelevant, even if Apple did sell to all areas of the World (which it does not). Whether Apple has 12% of the US market, or 40% of the K12 education market in the US, or 45% of our Research Institution, any other market share, is also irrelevant. The one relevant statistic for me is that of the 4 sorters and 6 bench-top cytometers in our 'Research Flow lab', 80% of them are running Macs and Cellquest. I suspect similar situations can be found elsewhere. I do not think we need to get into 'Mac vs PC' or 'BD versus Coulter' stuff. DIVERSITY IS IMPORTANT!!
While BD have taken a bit of flak over their failure to provide timely upgrades to the software that most BD/Mac combinations run on, it is my understanding that the software at the heart of the Coulter operating system is still (for almost all users) DOS based. It was not until the recent (I think) availability of 'EXPO' software, that you could even run Windoze on an XL/PC combo. (I suspect the ability to do the latter might not be seen by some as a step forward). Even so, we regularly take listmode files from my collaborators (who have a Coulter/PC set-up) and publish them in color via FACSConvert and Cellquest on my Mac. At the end of the day, the 'Figure' is simply superior.
Regardless, without both manufacturers competing for sales, things would be far far worse for us consumers. Its the same with Mac versus PC. Whether you use either or both platforms, only knuckleheads would disagree that DIVERSITY IS IMPORTANT!! After all, without Apple and others, who would Micro$oft get its ideas from? Like Karim, who stated "I've worked on both platforms and believe me I wouldn't even think about doing my FACS work (or any other daily lab tasks) on an NT machine", I hope BD continue to support the Mac OS (and improve it!).
Finally, in his latest post, Paul asks 'how long will the Mac be around?' I do not think that the 'pin-striped reptiles of Wall Street' should be influencing your short and medium term purchasing decisions. Did you also notice that Intel was down over 50% from its yearly high, Dell too, Micro$oft too, etc., etc. Has anyone on Wall Street 'written off' these companies? Of course not. What happened that made Apple's previous valuation of $17.39 billion suddenly drop to $8.4 billion? As detailed elsewhere (http://macweek.zdnet.com/2000/10/01/1005everywallst.html) Apple only made about $1.9 billion this year, instead of just over $2 billion It only grew about 20 percent this year, instead of a little more. "Oooh, scary stuff -- the company is doomed, doomed I say! Sell now while you still can. This crosses the line from stupidity into insanity". Lets support diversity (and keep G4 Cubes out of Mario's flow lab, - I hate it when coffee gets spilled into the CD-Rom reader!!).
My very last 2c worth.
Rob Sutherland University Health Network University of Toronto Received on Tue Oct 10 11:29:07 2000 • This message: [ Message body ] • Next message: Houston, Jim : "Hoescht 33342 and UV Excitation with 325nm HeCd Laser" • Previous message: Bob Faust: "Position Open" • Next in thread: D. Robert Sutherland: "re: RE: G4 for FACSCalibur - and a plug for Mario!! and one more comment.." • Maybe reply: D. Robert Sutherland: "re: RE: G4 for FACSCalibur - and a plug for Mario!! and one more comment.." • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:38 EST
G4 for FACSCalibur - and a plug for Mario!! • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] From: Reed, Doug S Dr USAMRIID <Doug.Reed@DET.AMEDD.ARMY.MIL> Date: Tue Oct 10 2000 - 14:28:11 EST Having read the great PC vs Mac debate going on here, I thought I'd throw in my two cents worth on the whole matter. The whole issue of who's computer is best comes down (really) to personal preferences.
Personally I have never understood the appeal of a Mac, and I've owned computers since the heyday of the Trash System 80s. But to each his or her own. It would certainly be nice if BD were to offer its customers the option of Windows, Mac, BeOS or Linux, but that seems unlikely in the extreme. I seriously doubt BD would invest the development time required, because the expense would not justify it. And don't hold out for a Java-based CellQuest - the list of Java applications that "run anywhere" are very few indeed. Java may someday be capable of such feats, but for now it is far to sluggish to handle real-time acquisition on a cytometer.
The biggest advantage to me for using a Mac instead of a PC on the Calibur is that no one (here anyways) will be using it for anything other than FACS.
:^) Douglas S. Reed, Ph.D. Department of Aerobiology and Product Evaluation Division of Toxinology & Aerobiology U.S. Army Medical Research Institute of Infectious Diseases 1425 Porter St. Ft. Detrick Frederick, MD 21702 (301) 619-6728 voice (301) 619-2541 fax doug.reed@det.amedd.army.mil Received on Wed Oct 11 13:03:32 2000 • This message: [ Message body ] • Next message: Julie Auger: "chromosomes" • Previous message: Tom Mc Closkey: "best multicolor instrument?" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:39 EST Re: Mac Notebooks for Cellquest • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] [ Replies ] From: Adrian Smith <A.Smith@centenary.usyd.edu.AU> Date: Tue Nov 14 2000 - 19:07:27 EST
>We are interested in buying a MAC for off-line analysis. Since we have a >shortage of desk/bench space, there is interest in perhaps getting a [quoted text clipped - 8 lines] > >Doug If you are going to buy another Macintosh specifically for analysis then you will need to buy another license for CellQuest. In that case before you buy make sure you consider FlowJo as an alternative analysis package to CellQuest. We have found it much better and there have never been any compatibility problems - I'm running it right now on a Powerbook. I have not tried CellQuest on a Powerbook. (All our acquisition is done on a FacStarPlus with a Mac running CellQuest, but I haven't used CellQuest for analysis since FlowJo was released in 1997).
In terms of saving desk/bench space, you might also consider the G4 Cube - it really is a tiny computer and if you coupled it with an LCD monitor it would take up not much more space than a laptop (I have no idea how that would compare price wise though - in Australia it would end up being about the same I think). The Cube will run FlowJo slightly faster but there is not a big difference between a G4/400 (Cube) or the G3/500 in the top of the line Powerbook (rumour has it that the Powerbook line will be significantly revised in January if you can wait that long...).
The big disadvantage of going small (whether laptop or lcd monitor) is the limited screen space you can use. Both CellQuest and FlowJo benefit from more screen space, ie larger monitors. However, the best way I have found to increase screen space is to add a second monitor. The Powerbook has the advantage here as it supports a second monitor straight out of the box - at the moment I'm running the powerbook with a 15inch monitor sitting beside it and the Mac desktop expands over both of them. (It is more difficult to add a second monitor to the Cube as it involves finding a single video card that will support dual monitors; if you bought the normal G4 Tower you would simply add a second PCI video card) Of course once you start adding second monitors they start taking up more space.
The other thing to make sure is that you get sufficient RAM. Some of the base Mac models ship with only 64MB. This may be enough depending on the analysis you do but I think 128MB or more is much better (our lab G4s which are used for analysis have 192MB).
Don't hesitate to ask any more questions...
Adrian Smith Received on Wed Nov 15 11:32:16 2000 • This message: [ Message body ] • Next message: Haywood Pyle: "Lipemic Samples" • Previous message: FACS_COPY@wehi.edu.au: "Re: Autoloaders for Elite/Altra" • In reply to: Douglas S. Smoot: "Mac Notebooks for Cellquest" • Next in thread: Weiwen Shen: "about CD59 detection" • Reply: Weiwen Shen: "about CD59 detection" • Reply: Karim Vermaelen: "Re: Mac Notebooks for Cellquest" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:43 EST
Re: Mac Notebooks for Cellquest • This message: [ Message body ] [ More options ] • Related messages: [ Next message ] [ Previous message ] [ In reply to ] From: Karim Vermaelen <Karim.Vermaelen@rug.ac.be> Date: Thu Nov 16 2000 - 08:49:27 EST Hi, Like Adrian, I've had good experience doing intensive off-line analysis of flow data on a Powerbook G3 laptop running FlowJo (check out www.treestar.com for prices and licensing options; you can get a free limited-time demo license for a test-drive). The FlowJo license we bought currently runs Flowjo 2.7.8 on a 266 Mhz "beige" desktop G3, under MacOS 9.0.4 and multiple-users ON (OSX is still in beta testing phase and neither Cellquest nor FlowJo are native OSX applications today). I often have to acquire >100000 events/5 parameters per sample for my experiments but the analysis really flies on this G3, and it's even snappier on my 300 Mhz G3 laptop. I even (BD's gonna hate that) performed event acquisition straight from the FACStation (an old upgraded Quadra) to the Powerbook over Ethernet. With the new G4 tower FACStations and Powerbooks (besides more processing power) you have the added option of wireless networking for transfer/backup of data after acquisition. Check out www.apple.com/airport for a clear explanation. As for the PC issue, our dept. is primarily a PC network with a file server running Novell Netware. Netware can talk to Appletalk networks through a user-transparent interface on the Mac side (NT provides this too, albeit slower as far as I've heard). We used to back up gigs of FCM data from the Mac network to a PC file server without any problem. Feel free to mail for any additional questions Karim
> >We are interested in buying a MAC for off-line analysis. Since we have a > >shortage of desk/bench space, there is interest in perhaps getting a [quoted text clipped - 8 lines] > > > >Doug Received on Thu Nov 16 13:32:15 2000 • This message: [ Message body ] • Next message: Richard K. Meister: "Dead cells" • Previous message: Steve G. Hilliard: "magnetic beads and valves?" • In reply to: Adrian Smith: "Re: Mac Notebooks for Cellquest" • Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:45 EST
>ISAC XXII International Congress & Purdue Cytometry University ML & >XML [quoted text clipped - 1347 lines] > >http://www.new.facebook.com/note.php?note_id=23879772873&ref=mf
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