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Medical Forum / Diseases and Disorders / AIDS / December 2006

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HIV sequencing protocol

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abinkum@yahoo.com - 02 Jun 2004 04:22 GMT
I still haven't got around to reading up on the method used to achieve
the claimed sequencing of HIV. I wonder if anyone here can outline the
protocol that was used and/or the relevant paper(s).

- James
Nick Bennett - 09 Jun 2004 11:44 GMT
It takes about 5 minutes to find this, from scratch.  The reference genome
for HIV-1 is HXB-2, Genbank accession k03455.  You find this using a
nucleotide search from PubMed with the terms:

HIV1 reference genome (note that HIV-1 brings up a shed load of fruit fly
DNA!)

Then scroll down to find the reference which gives the entire genome
(1-9635, most of it anyway) and click on the link.

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=8
7299196&dopt=Citation


AIDS Res Hum Retroviruses. 1987 Spring;3(1):57-69.

Complete nucleotide sequences of functional clones of the AIDS virus.

Ratner L, Fisher A, Jagodzinski LL, Mitsuya H, Liou RS, Gallo RC,
Wong-Staal F.

Is your goal.  Sadly it's from 1987 and doesn't have an online reference.

If you like I can try to dig it up, since I doubt it's readily available
in a public library.

Note that an in vitro model was developed before they had sequenced the
virus...standard practise, especially in pre-sequencing days!!!

Bennett

> I still haven't got around to reading up on the method used to achieve
> the claimed sequencing of HIV. I wonder if anyone here can outline the
> protocol that was used and/or the relevant paper(s).
>
> - James
Tony Lance - 12 Dec 2006 17:08 GMT
Big Bertha Thing coil
Cosmic Ray Series
Possible Real World System Constructs
http://web.onetel.com/~tonylance/coil.html
Access page JPG 9K Image
Astrophysics net ring access site
Newsgroup Reviews including alt.politics

Two drawings of a Gyroscopic Top complete with coil.

Extract from Introductory Chapter;-
The fact is that the toe is magnetic, and this being the case
it is easy to see that the rolling of the toe on the side
of the metal produces the motion. But Figs. XV. (a) and (b)
illustrate a top, whose spindle behaves in exactly the same
manner as the toe of the spider top, and yet is in no way magnetic.
The action is purely mechanical, as we shall explain in later pages.
It is an attractive top to watch, especially as it rushes round
the corner when it comes to the end of the coil.

From the book
An Elementary Treatment of the Theory of
Spinning Tops and Gyroscopic Motion.
By Harold Crabtree M.A.
Formerly Scholar of Pembroke College, Cambridge
Assistant Master at Charterhouse
Longmans, Green and Co. 1923
First Edition 1909
Second Edition 1914
New Impression 1923
(C) Copyright Tony Lance 1998
Distribute complete and free of charge to comply.

Big Bertha Thing fiction

Anything but a fact, changes the face of twentieth century science.
9. Gyroscopic Top motion along coil masks a weaker field effect.

The Japanese antigravity experiment only worked one way.
With the object spinning one way OK, but the other way failed.
Anything but a fact...
10. One way gravity reduced and other way gravity increased,
   so is bi-polar field efftect.
11. No antigravity, which is not bi-polar, so contradicts
   bi-polar field effect.

Wave Particle Duality Paradox two slit interference patterns
can be disrupted by;-
1. Passive sensor near 2nd slit.
2. Turning room light on.
Anything but a fact...
12. LCD digital watch, without backlight, near 2nd slit.
13. Thicker wall between slits.

Redshift is both speedometer and tape measure.
According to redshift speedo, the universe has a go faster
stripe painted on the outside.
Anything but a fact...
14. No Big Bang, which needs go faster stripe on the inside
or antigravity.
15. No redshift speedo due to Steady State, which needs
neither to have go faster stripe.

Wave Particle Duality needs 1 slit and 2 slit experiments
to give different results from same effect. Make the walls
inside and outside the slits the same size and centre of
gravity.
Anything but a fact...
16. Centre of gravity is at slit in one, but not the other.
17. Equal forces act on the slit in one, but not the other.
18. Wave Particle Duality as a field effect gives different
results from same effect.

Tony Lance
tonylance@myinternetuk.com

Big Bertha Thing handcart

1. Gardening section of Daily Telegraph, on Saturday
   17th November 2001, shows full front page spread
   picture of pin-wheel rickshaw.
2. For another picture see;-
   http://web.onetel.com/~tonylance/pinwheel.html
3. Both show load perfectly distributed.
4. Both are idealized pictures of a rickshaw that does
   not exist.
5. A 19th century cheque-book journalist requested a
   picture of an unnusual rickshaw, which was promised
   for tomorrow.
6. A portrait painter, a landscape artist and a
   cartoonist submitted quotes, which the cartoonist won.
7. A wheelborrow uses straight arm technology.
8. In common with a handcart, the so called pin-wheel
   rickshaw, uses bent arm technology.
9. It is physically impossible and so unviable.
10. This is a scientific cartoon, some work, some don't.
 
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