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Medical Forum / Diseases and Disorders / AIDS / May 2008

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ISAC Congress Keep Reading Purdue Cytometry Mail List     MARKETING...President J Paul Robinson......WE ARE NOT SCAMMERS

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Mitch Haynes - 21 May 2008 06:55 GMT
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> Related messages: [ Next message ] [ Previous message ]
> From: VSH - Tech Support <t...@vsh.com>
> Date: Wed Jun 30 2004 - 12:08:14 EST
> David
> At the risk of sounding commercial, WinList can do what you want. I
> have
> attached a few pictures showing the WinList Region Array feature.
> With this
> you can tell the program exactly how you want the regions created and
> how
> many. If you would like to try it for yourself you can download a
> trial
> version from our web sitewww.vsh.com.
> Please let me know if you have any questions.
> Best regards
> Don
> Donald J. Herbert
> Technical Support Manager
> Verity Software House, Inc.
> PO Box 247
> 45A Augusta Road
> Topsham, ME, USA 04086
> Phone: (207) 729-6767 ext.190
> Fax: (207) 729-5443
> email: t...@vsh.com
> web:www.vsh.com

   * 1 week ago

Additional Details

1 week ago
anti) Commercial Posting:)
> This message: [ Message body ] [ More options ]
> Related messages: [ Next message ] [ Previous message ]
> From: Ray Hicks <rh...@cam.ac.uk>
> Date: Wed Oct 13 2004 - 08:43:13 EST
> Just a quick nopte to let people know that FCSPress is now shareware
> (no time restrictions or feature reductions for free use, though if
> you feel like like paying for it you can using paypal). It also now
> works with Diva export files and runs ok under classic on OS 10.2 and
> 10.3. I've discontinued FCS Assistant, which has fallen behind
> FCSPress remarkably, and which failed to run as shareware on some
> systems (it insisted you buy it or refused to run - FCSPress doesn't
> even have a nag screen now - pesky things).
> FCSPress is available fromwww.fcspress.com
> Enjoy!
> Ray

1 week ago
From: VSH Tech Support [mailto:T...@vsh.com]
> > Sent: Wednesday, February 21, 2007 10:30 AM
> > To: Cytometry Mailing List
> > Subject: RE: DNA analysis software
> > Hello Ibtissam,
> > ModFit LT, for PC or Mac, has advanced modeling capability for
> > research applications in DNA cell cycle analysis. You may use any of
> > the model templates the program offers, or create your own models for
> > non-traditional analysis, including non-mammalian DNA cell cycle
> > studies. ModFit LT can be linked to our WinList program to provide a
> > complete cell cycle analysis on any number of sub-populations with a
> > single click of a button.
> > For an overview, visithttp://www.vsh.com/products%a0.
> > Best regards,
> > Don
> > Donald J. Herbert
> > Technical Support Manager
> > Verity Software House

1 week ago
FW: FlowJo Seminar for Los Angeles Basin
From: Barsky, Lora (LBar...@chla.usc.edu)
Date: Tue Mar 20 2001 - 14:28:04 EST

Next message: PLo...@adarc.org: "Apoptosis, Annexin V and Ficoll"
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[ attachment ]

--------------------------------------...

I'm not sure if there is a Los Angeles Basin users
group,
but thought I could advertise here to interested parties.
Thanks,

Lora Barsky - Lead Operator
Research Immunology/BMT FACS Core
Childrens Hospital Los Angeles

1 week ago
Re: Software
From: Ray Hicks (rh...@cus.cam.ac.uk)
Date: Fri Jul 04 1997 - 04:15:27 EST

Next message: Woo, Gary: "BrdU staining"
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--------------------------------------...

Hi Vincent,

I'm developing an analysis program, based on FCS Assistant, called
FCSPress. So far it's quite rudimentary and only allows you to plot
histograms. It doesn't do gating or analysis yet. And there is little
support for the edit menu functions (cut, copy, paste, undo). Unlike
most
cytometry programs available on the mac, it allows you to open a data
file
and optionally manipulate its contents, graphs are plotted from the
active
data window into graph windows,

1 week ago
Document count: purdue (139318) cytometry (25468) mail (53709) list
(63448) 2007 (37553) verity (222) software (15216) sales (14402) 07
(21574) by (129519) thread (26375) purdue cytometry mail list 2007
verity software sales 07 by... (27962) about 187244 results found, top
500 sorted by relevance score using date hide summaries group by
location spacer 1-10 next Purdue Cytometry Mailing List: RE: DNA
analysis software ... Purdue Cytometry Mailing List: RE: DNA analysis
software ... J. Herbert Technical Support Manager Verity Software
House ... http://flowcyt.cyto.purdue.edu/hmarchiv/... -

1 week ago
Cytometry Mailing List: Re: DNA analysis software ... vsh.com] > Sent:
Wednesday, February 21, 2007 10:30 AM > To: Cytometry Mailing List >
Subject: RE: DNA ... J. Herbert > Technical Support Manager > Verity
Software House ... http://www.cyto.purdue.edu/hmarchiv/Curr...
- 8.6KB 78% |||||||||||||||||||| 26 May 07 Find Similar Highlight
Purdue Cytometry Mailing List: Announcing User Forum at Verity
Software House, Inc. ... Purdue Cytometry Mailing List: Announcing
User Forum at Verity ... Verity Software House is pleased to announce
the creation of a Verity User's Forum on our ...

1 week ago
Purdue Cytometry Mailing List: DNA analysis software
> ... a conversion at no charge. I invite you to contact Verity
> Software
> House directly with any specific questions or issues. Best
> regards ...
> issues. Best regards, Don Donald J. Herbert Technical Support Manager
> Verity Software House ...http://www.cyto.purdue.edu/hmarchiv/Curr... 5.6KB 69%
> ||||||||||||||||||||
> 26 May 07
> Find Similar
> Highlight

1 week ago
On Aug 20, 2004, at 2:25 PM, J. Paul Robinson wrote:
> > > ummm....Mario says..
> > > "In life sciences -- particularly in research life sciences --
> > > probably 50 to 70% of research laboratories used
> > > Macs"....while I have a passionate dislike for Windows......is
> > > this really true ??? or is the key word there "used"?? (Ok...I
> > > have put on my helmet and armor....waiting...)
> > > paul
> > >> For all of the mr groupies out there in cytometry cyberspace. Don't
> > >> wet your
> > >> pocket protectors over this.
> > >> Honestly though, well deserved praise for Mario & the Tree Star group:
> > >>http://www.apple.com/science/profiles/ro...

1 week ago
Purdue Cytometry Mailing List: Confirm Your Bowdoin Course Regis
You may email us at s <BLOCKED::mailto:Sa...@vsh.com> a...@vsh.com to
confirm your status. Best regards, Verity Software House 45A Augusta
Road Topsham, ...
flowcyt.cyto.purdue.edu/hmarchiv/2006/... - 6k - Cached - Similar
pages - Note this

1 week ago
To subscribe to the image cytometry mailing list, mail a subscription
request .... LABCAT Software Home Page has on- line sales and support
for a line of PC ...
www.urmc.rochester.edu/smd/pathres/Lon... - 208k - Cached - Similar
pages - Note this

1 week ago
Purdue Cytometry Mailing List: ModFit LT 3.1 Service Pack 1 now ...
For a complete description, see CYTOMETRY 53A:2, 103-111 (June,
2003). ... Best regards, Mark Mark E. Munson Sales Manager Verity
Software House, ...
www.cyto.purdue.edu/hmarchiv/2003/1134... - 6k - Cached - Similar
pages - Note this

1 week ago
Why were all these links removed from Verity Soft Ware House ...
(21574) by (129519) thread (26375) purdue cytometry mail list 2007
verity software sales 07 by... (27962) about 187244 results found, top
500 sorted by ...

1 week ago
30 Jan 07
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Purdue Cytometry Mailing List: Re: Thanks for the suggestions -
... http://www.wehi.edu.au/cytometry/WEASELv... > > Winlist 3d
from
Verity House Software (www.vsh.com) ... Yes, I live off FlowJo sales,
and that's a blatantly ...
http://www.cyto.purdue.edu/hmarchiv/2006... - 8.5KB
71%

1 week ago
Cytometry Software
Purdue University Cytometry Laboratories. Catalog of Free Flow
Cytometry Software ... WinMDI Software - Joe Trotter. FCS Assistant -
Ray Hicks. FCS Press - Ray Hicks ...
www.cyto.purdue.edu/flowcyt/software.h... - 8k - Cached

1 week ago
Purdue Cytometry Mailing List: Cytometry software for linux
... Purdue Cytometry Mailing List: Cytometry software for linux ...
Previous message: Verity Software House: "RE: FL2H in PI cell cycle
analysis" ...
http://flowcyt.cyto.purdue.edu/hmarchiv/... - 5.5KB
75%

13 Sep 07

1 week ago
From: Adam Treister (a...@treestar.com)
Date: Mon Apr 22 2002 - 00:15:07 EST
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[ attachment ]
______________________________________...
Only two more weeks until ISAC, that biennial bacchanalia of flower
power
and fun! So I hope you'll excuse a bunch of blatantly commercial
announcements to the list, but endulge me this one time and read on.

At the show we'll be releasing FlowJo Version 4. We've got new
platforms
for overlaying and clustering, we've made it work better across the
Internet, added all sorts of new

1 week ago
For those who haven't found it yet, we've unveiled a spanking new
FlowJo
website. Flowjo.com is chuck full of new content, functionality and
spunk.
Automated price quotes, online ordering, a FAQ that will guide you to
new
depths of understanding, and none of that awful yellow on black text.
The
search engine even works. No ads & cookie-free. Check it out.

Specifically, you should check our pricing. Prices are going up on
May 10.
It has been a number of years since we've changed our prices and with
the
development of the OSX and PC versions, it¹s time for a leap. I
guess
there's no such thing as a free launch. Anyway, this may be a great
time to
buy that FlowJo ten pack you've been thinking about. Licenses
purchased
before May 10 are entitled to a year of free upgrades, including the
4.0
release.

Unleash the flower power!

Adam
--
Adam Treister
Tree Star,

1 week ago
Fluorescence Compensation
Jennifer Wilshire, PhD jenni...@flowjo.com MetroFlow Meeting,
Princeton, NJ .... Purdue Cytometry Mailing list - http://www.cyto.purdue.edu.
Practical Flow ...
www.metroflow.org/archive/2004Jan06/ab... -
39k - Cached - Similar pages - Note this

1 week ago
Purdue Cytometry Mailing List: Re: Nonreadable FCS 3 files from
From : J. Paul Robinson <j...@flowcyt.cyto.purdue.edu> ... For data
analysis we want to > use a centraly designed templates made in Flow
Jo software. ...
www.cyto.purdue.edu/hmarchiv/2004/0997... - 8k - Cached - Similar
pages - Note this

1 week ago
Useful Links & Resources - Flow Cytometry Site
General. Purdue University Cytometry Labs (J. Paul Robinson) ...
Software. FlowJo. http://flowjo.com/. FACSPress and FACSAssistant.
http://www.fcspress.com/ ...
idi.harvard.edu/content/facilities/cyt...
- 42k - Cached - Similar pages - Note this

Meeting 2007
J Paul Robinson (Purdue University, USA). Multicolour Flow
Cytometry ... Cytek Development, Distrilab, FlowJo, Guava
Technologies, Invitrogen, ...
www.flowcytometryuk.org/fliers/Annual1... - 8k - Cached - Similar
pages - Note this

1 week ago
Flocyte course in Mexico City, march 3 to 7th 2008
This message: [ Message body ] [ More options ]
Related messages: [ Next message ] [ Previous message ]
From: Candela Iglesias Chiesa <candela.igles...@cieni.org.mx>
Date: Thu Jan 03 2008 - 11:39:14 EST

Para mensaje en espaqol, ver mas abajo. For message in Spanish, see
below.

Dear flowers,

The Flow Cytometry Core Facility at the Center for Research in
Infectious
Diseases (CIENI), at the Instituto Nacional de Enfermedades
Respiratorias in
Mexico City is pleased to host the first FloCyte Regional Training
Program
in Mexico! We are offering a full week of Flow Cytometry courses in
March
2008. Dorothy Lewis and Carol Oxford will be our instructors for the
5 days
of courses. You can register for each individually or receive a
special
discount, and register for all five days for $200 off the regular
price!
Please note that the courses will be held in English, but we'll have
translate

1 week ago
The Basic Flow Cytometry course will be held March 3-4, 2008. This
session
is excellent for beginners, and a great course for intermediate-
advanced
users looking for a stronger foundation in flow cytometry basic
theory.
Link to the course curriculum:
<>http://www.flocyte.com/FRTP/basic_curric...
http://www.flocyte.com/FRTP/basic_curric...

The Multiparameter Flow Cytometry and Compensation course will be
held
March
5, 2008. This advanced level course is for flow cytometry users who
are
interested in continuing their education in the field, and who plan
to
do
sophisticated polychromatic flow cytometry experimental analyses. It
is
suggested that participates have a good foundation in basic flow
theory and
operation prior to attending this course, or have attended the basic
course.
Link to course curriculum:
<>http://www.flocyte.com/FRTP/multiparamet...
http://www.flocyte.com/FRTP/multiparamet...

1 week ago
Please invite others you know who would also benefit who may not be
on
the
Purdue List Server.

1 week ago
Visit the website for more information on the FloCyte Associates
Regional
Training Program. <>http://www.flocyte.com/FRTP/FRTPHome.htm...
http://www.flocyte.com/FRTP/FRTPHome.htm
Also, as the hostess of this event, please feel free to contact me
for
more
details.

Hope to see you there!

Marma Candela Iglesias, PhD

Centro de Investigacion en Enfermedades Infecciosas (CIENI)

Instituto Nacional de Enfermedades Respiratorias

Calzada de Tlalpan 4502. Col. Seccion XVI

CP 14080. Deleg. Tlalpan

Mixico DF.

tel y fax +52 (55) 56 66 79 85

cand...@iner.gob.mx

candela.igles...@cieni.org.mx

1 week ago
Pueden encontrar mas informacisn sobre el Programa Regional de
Entrenamiento
de la Asociacisn Flocyte en http://www.flocyte.com/FRTP/FRTPHome.htm

Asimismo, como la anfitriona de este evento, estoy a su disposicisn
si
requieren mas detalles.

!Espero verlos en el curso!

Saludos cordiales,

Marma Candela Iglesias, PhD

Centro de Investigacion en Enfermedades Infecciosas (CIENI)

Instituto Nacional de Enfermedades Respiratorias

Calzada de Tlalpan 4502. Col. Seccion XVI

CP 14080. Deleg. Tlalpan

Mixico DF.

1 week ago
Received on Thu Jan 3 18:38:00 2008
This message: [ Message body ]
Next message: Susan DeMaggio: "Budapest on HDTV - a little off the
flow topic, but relevant"
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PBMCs
results in sticky cells - The replies"
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1 week ago
Only two more weeks until ISAC, that biennial bacchanalia of flower
power and fun! So I hope you'll excuse a bunch of blatantly commercial
announcements to the list, but endulge me this one time and read on.
At the show we'll be releasing FlowJo Version 4. We've got new
platforms for overlaying and clustering, we've made it work better
across the Internet, added all sorts of new conveniences, and spiffed
it up with the new OS X look and feel. What was already the best
analysis software in flow cytometry has gotten a whole lot better. At
the meeting, Tree Star is proud to be sponsoring the CyberCafe, your
link to home and responsibility while you're in carefree San Diego.
Check your email, surf the web, download the slides you'll be
presenting at ten. A cadre of California companies has ontributed to
bring in a premier local roaster to satisfy all your latte urges. We
hope you'll all drop by and see what we mean when we say we're
committed to Java.

1 week ago
The network is
going
wireless this year. Just pop a 802.11 card in your laptop, and while
your
neighbor plays solitaire through the keynote, you can be reading e-
mail.

We're going to open the CyberCafe with the Second Biennial FlowJo
Users
Group Meeting,. Saturday night May 4 at 8PM. The first user group
meeting
was cancelled for lack of interest when Dave Novo brought in a case
and a
half of French wine, so this year we're going to try real hard to
assemble
to the point where we can see the show of hands on something before we
disband in search of alcohol. We'll have a whole bunch of Macs
running
FlowJo v4 under OS X, and you can bring your own data and get into big
arguments about compensation. Be the first to get the newest FlowJo
t-shirt.
All you Windows fans out the**re, come by our booth to see FlowJo
running on a PC! We'll be previewing the long awaited Java version of
FlowJo. We're still not ready to release it,

1 week ago
but we'll be giving a peak as to what
it is going to do.
For those who haven't found it yet, we've unveiled a spanking new
FlowJo website. Flowjo.com is chuck full of new content, functionality
and
spunk.
Automated price quotes, online ordering, a FAQ that will guide you to
new depths of understanding, and none of that awful yellow on black
text.
The search engine even works. No ads & cookie-free. Check it out.
Specifically, you should check our pricing. Prices are going up on
May 10.
It has been a number of years since we've changed our prices and with
the development of the OSX and PC versions, it¹s time for a leap. I
guess there's no such thing as a free launch. Anyway, this may be a
great
time to buy that FlowJo ten pack you've been thinking about. Licenses
purchased before May 10 are entitled to a year of free upgrades,
including the 4.0 release.Unleash the flower power!
Adam Adam Treister
Tree Star, Inc.
ph: 800-366-6045

1 week ago
fax: 1-650-508-9186
a...@treestar.com www.treestar.com
--------------------------------------...
______________________________________...

1 week ago
From: Novo, David <david.n...@denovosoftware.com>
Date: Fri Aug 17 2007 - 18:52:53 EDT

Hello Kate,

The FC 500 data files are indeed a complicated beast that give you a
lot
of information. There are indeed two separate data sets inside the one
FC500 file. This is allowed by the FCS standard.

The first data set is a FCS 2.0 file. This is quite a typical FCS 2.0
file, stored in 10 bit with the data already saved compensated and
converted to log (if necessary). This is quite clever to put it there
because any analysis software that can read FCS 2.0 files should be
able
to read this file, and not even know that there was a second data set
hiding afterwards. I am not sure why the BD CBA software would not be
able to handle this, maybe there is a hidden setting or something that
allows this.

1 week ago
The second data set is a high resolution data set that is in FCS 3.0
format. This contains all the high resolution linear data, that is
stored uncompensated, and is ideal for performing software
compensation.
I guess our web site is either too high, or too low, and fell out of
the
range of your previous search :-) FCS Express can read both the FCS
2.0
and FCS 3.0 portion of the FCS 500 data files. You can even export
them
as individual files if you like. When loading the files you can either
default to load the FCS 2.0 or FCS 3.0 portion, or be prompted which
to
choose.

I also know that WinList can read both portions of the FC500 file. I
am
not familiar with FlowJo's capabilities in this regard. If you contact
them, I am sure they will be glad to answer this point.

-Dave

-----------------------------------

David Novo

President

De Novo Software

david.n...@denovosoftware.com

1 week ago
From: J. Paul Robinson <j...@flowcyt.cyto.purdue.edu>
Date: Mon Jun 04 2007 - 23:40:22 EDT
Dear Colleagues
We are almost finished one of the most exciting projects we have
done.
It is our new DVD "Cytometry - 60 years of Innovation". It's our
first DVD. Actually, it's a two DVD set - one DVD works on your DVD
player
and
the other on your computer. The contents are really fantastic -
original
video of Mack Fulwyler explaining how he invented the cell sorter,
including pages from his original lab notebook on how he came up with
the ideas...Len Herzenberg showing how he developed the technology
into
something that impacted the whole world. Howard Shapiro - one of us
who
has contributed his life to the field, as well as bringing the key
technology comes to you in song! Dozens of the leaders of the field
tell
us how they see cytometry in the past, present and future. Of course,
there are lots of educational features on the disc as well -
lectures,

1 week ago
powerpoint files galore, and a plethora of educational materials to
enhance your role as a student, staff member, teacher or core
manager.

The way we bring these educational discs free to you all is via our
sponsors. Sony has graciously donated the production from the Indiana
plant - you probably didn't know that your downloaded songs, dvds and
cds are probably mostly produced in good ole' Indiana - but they
are!!

1 week ago
This 60 year celebration of cytometry - brings Diamond Sponsors Bangs
Labs and Beckman-Coulter who lead our sponsors. Both companies bring
a
depth of knowledge and skills to cytometry. Beckman-Coulter started
the
field with the invention by Wallace Coulter of the Coulter counter
over
50 years ago. Bangs Labs has been a leader in bead technology for
many
years. Both companies have been two of our strongest supporters over
the
many years of doing these educational projects. Thank you for that
support.

1 week ago
Major sponsors who generously supported this initiative include
Partec,
Invitrogen, Dako, Guava and John Wiley & Sons, publisher of Cytometry
Part A. These major players in the field of cytometry are known to
all
of you.

We also acknowledge support from Hamamatsu, Southern Biotech,
Compucyte,
Phoenix Flow systems, Spherotech, Accuri Cytometers, EXBIO
Praha,A.S.,
Duke Scientific, Coherent, Q-Imaging and Verity Software. All of
these
companies provide quality cytometry related resources and we are very
grateful for their support of this really exciting educational and
historic project.

1 week ago
Finally, I want to give one last opportunity for any of you to submit
materials that might enhance this project. We have a few days to go
before the project is literally "etched" in plastic! Send your
materials
to Monica Shively, the producer of this project.
(mon...@flowcyt.cyto.purdue.edu ). If you want to see a sneak preview
of
the project, go to http://www.cyto.purdue.edu/flowcyt/cdser...

We will be distributing 10,000 copies of this two DVD boxed set - I
hope
you all get a chance to get a copy. Please ask your sales rep from
one
of the above companies they will all have copies for distribution.

1 week ago
They
are the best source of this historic DVD set. Purdue University
Cytometry Laboratories, who also bring you this discussion board are
pleased to continue bringing innovative projects on cytometry to you.
I
hope you enjoy this one. It's the best we have ever done!

Kind regards

J. Paul Robinson
Director, Purdue University Cytometry Laboratories

1 week ago
--
J. Paul Robinson
SVM Professor of Cytomics
Professor of Immunopharmacology & Biomedical Engineering
Director, Purdue University Cytometry Laboratories
President, International Society for Analytical Cytology

Purdue University Cytometry Laboratories
Bindley Bioscience Center
1203 West State Street
Discovery Park, Purdue University
West Lafayette, IN 47907-2057
Ph (765) 494 0757; Fax (765) 494 0517
email: j...@flowcyt.cyto.purdue.edu
www.cyto.purdue.edu

--
J. Paul Robinson
SVM Professor of Cytomics
Professor of Immunopharmacology & Biomedical Engineering
Director, Purdue University Cytometry Laboratories
President, International Society for Analytical Cytology

Purdue University Cytometry Laboratories
Bindley Bioscience Center
1203 West State Street
Discovery Park, Purdue University
West Lafayette, IN 47907-2057
Ph (765) 494 0757; Fax (765) 494 0517
email: j...@flowcyt.cyto.purdue.edu
www.cyto.purdue.edu

1 week ago
Join ISAC - www.isac-net.org

Change lives today - www.cytometryforlife.org

1 week ago
Purdue Cytometry Mailing List: RE: CFSE graphs (UNCLASSIFIED)
... Verity's MODFIT software has a very nice tool for ... The
information contained in this electronic e-mail transmission and any
attachments are intended only for the ... http://flowcyt.cyto.purdue.edu/hmarchiv/...
- 9.3KB

52% ||||||||||||||||||||

23 Dec 07
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1 week ago
Purdue Cytometry Mailing List: Thanks for the suggestions - was
... Purdue Cytometry Mailing List: Thanks for the suggestions -
was ... cytometry/WEASELv2.html Winlist 3d from Verity House Software
(www.vsh.com) ...
http://flowcyt.cyto.purdue.edu/hmarchiv/... - 6.6KB

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12 May 06
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1 week ago
* Software recommendations for offline analysis in PC-land
o VSH - Tech Support (Mon Oct 25 2004 - 13:53:09 EST)
o Cytometry (Tue Oct 19 2004 - 23:42:40 EST)
o Zand, Martin (Mon Oct 18 2004 - 15:05:30 EST)
RE: Software recommendations for offline analysis in PC-land
* This message: [ Message body ] [ More options ]
* Related messages: [ Next message ] [ Previous message ] [ Maybe in
reply to ]
From: VSH - Tech Support <t...@vsh.com>
Date: Mon Oct 25 2004 - 13:53:09 EST
Hello, Dr. Zand,

As a follow-up to your posting on the cytometry mailing list, I should
explain that only the first copy of WinList is $2000 (the "primary
license"), and after that all "additional user licenses" are only $400
each,
for the PC or for the Mac. We also offer a software "battery" option
that
allows you to use WinList for as little as $250.
Please contact me directly to discuss these options in more detail.

Best regards,
Mark

Mark E. Munson
Sales Manager
Verity Software

1 week ago
Purdue Cytometry Mailing List: Re: HP to Windows NT reader
... Rich: ... I have been very happy with Verity's WHPPC package. It
consists of an interface card on the ... It consists of an interface
card on the PC and software for both the PC and HP. It is very fast
(about 10X ...
http://www.cyto.purdue.edu/hmarchiv/1997... - 4.9KB

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1 week ago
Purdue Cytometry Mailing List: Re: FACS simulator?
... Verity did a good job, but with a different emphasis, on their
software ... Verity did a good job, but with a different emphasis, on
their software here http://www.vsh.com/downloads/Simulator/C... Hope
this helps Geoff ...
http://flowcyt.cyto.purdue.edu/hmarchiv/... - 7.0KB

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1 week ago
Re: SPAM-LOW: Re: Manual vs. Auto compensation
* This message: [ Message body ] [ More options ]
* Related messages: [ Next message ] [ Previous message ] [ In reply
to ] [ Next in thread ]
From: FloCyte Associates, INC <...@FloCyte.com>
Date: Wed Feb 13 2008 - 19:41:27 EST
Hi Mario
I have to commend YOU too! Two of the good explanation were from
FloCyte graduates - who learned from YOUR slides!!
Sue

1 week ago
At 11:54 AM 2/12/2008, you wrote:
Let me add one comment to this... Maciej is right that minor
miscompensation will not affect your results if you are gating on
easily-separable populations. It can strongly affect your results if
you are gating on difficult-to-separate populations ("smears").

Most importantly, it will INVALIDATE your results if you are relying
on MFI (fluorescence intensity) calculations. For those who are doing
MFI analyses, it is absolutely essential to validate your compensation
settings!

mr

(PS, it is really encouraging that I saw so many excellent
explanations of compensation on the mailing list recently! Really
outstanding.)

1 week ago
--- Maciej Simm <...@treestar.com> wrote:

Colleagues,

It's very difficult to answer the question "should I
do it by hand or
computer" without the whole context of the specific
scenario when one
fails and the other works.

Instead, we can focus on a broader question "is my
compensationcorrect?"

1 week ago
Mathematically, we can estimate validity of our
compensation matrices
by comparing medians - for example, the median of
the positives is
equal to the median of the negatives. Medians are
better than mean/
GM's because they aren't affected by outliers and
better represent
central tendency of your data.

1 week ago
Since biexponential transformation (aka logicle
display, etc..) became
popular in many software packages, there is an
easier way to 'eyeball'
bad compensation - enable this scaling and see if
your single-
negatives (some call them single-positives, I'm a
sheath tank half-
empty kinda guy ;) ) are symmetrically distributed
around the X=0 /
Y=0 lines similar to your double-negatives. If not,
there's either
over comp, or undercomp. Here's an example, with
hypothetical X/Y
parameter distributions:

1 week ago
http://cd4cd8.com/1.png proper comp -1 % - the X+/Y-
population leans
upward, suggesting undercompensation.
http://cd4cd8.com/2.png proper comp +1% - the X+/Y-
population is
sagging toward X axis. Over comp'd.
http://cd4cd8.com/3.png proper comp (correct values)
- the centers of
X-/Y- and X+/Y- are aligned.

hope this helps,

1 week ago
Maciej Simm
TreeStar Inc.

PS. I vote for pistachio ice cream.

I have people in the lab who complain about me
not doing manual
compensations and instead have the Aria calculate
them for me. And
when I have the Aria compensate, they want to
manually compensate
after they record the data because the plots
"don't look right." I
mean, isn't that simply producing "make-belief"
data? Or am I
missing something?

1 week ago
----Original Message-----
From: FloCyte Associates, INC [mai...@flocyte.com]
Sent: Thu 3/20/2008 2:16 PM
To: cyto-inbox
Subject: RE: Bay bioscience opens US office and introduces its JSAN
cell sorting and analysis system there

No this is not acceptable! I'm sorry, I just don't get it! I agree
100% with Roland.

I can't understand your position at all? WHO pays the majority of
the expenses for meetings? Vendors! Without them your cost to attend
ISAC would probably triple or quadruple!! Who allows you to have
very cheap or FREE local users' group meetings? Vendors! Who solves
issues with vendor services?? VENDORS! Why on earth would you
exclude vendors from the discussion???

1 week ago
And actually SOME people welcome messages from vendors... Vendors can
solve a lot of your problems! The Boston Area high speed sorter
list, for example, welcomes vendors responses and they get
results. The vendors HEAR their requests and respond! I've learned
a LOT from vendors! Without messages from Vendors, how do you find
out about new products and services? How often would you go to the
ISAC website to look for a new product you don't even know
exists? Or how do you know there is some training opportunity
happening in your area? OR how do Vendors know what you need and how
do you get your service / technical questions heard by all vendors?

1 week ago
Shutting them out isn't the answer to your mail problems! Roland's
answer was eloquent! And, although we've discussed this often,
nothing has ever evolved that is a better suggestion! If you don't
like messages from vendors, just filter them out, and having
[Company:] in the subject time is a perfect way to do that!

I'm sorry, I just don't get it!! Why would you shoot yourself in
the foot? Vendors - you can put my email address on your mailing
list!! Just put [Company:] in the subject line! I'll filter them
and decide what I want to read or not read later! Just like I filter
this list!
Sue

1 week ago
Received on Mon Mar 24 17:18:00 2008
* This message: [ Message body ]
* Next message: Ray Hicks: "FL-6 issues - a not-so-nonsensical
label!!"
* Previous message: Adrian Smith: "Re: Bay bioscience opens US office
and introduces its JSAN cell sorting and analysis system there"
* In reply to: FloCyte Associates, INC: "RE: Bay bioscience opens US
office and introduces its JSAN cell sorting and analysis system
there"
* Next in thread: bmcb...@uoguelph.ca: "RE: Bay bioscience opens US
office and introduces its JSAN cell sorting and analysis system
there"
* Reply: bmcb...@uoguelph.ca: "RE: Bay bioscience opens US office and
introduces its JSAN cell sorting and analysis system there"
* Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
Subject ] [ By Author ] [ By messages with attachments ]
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03:12:00 EST

1 week ago
From: <...@Coulter.ComDate: Fri Mar 21 2008 - 19:47:53 EDT
Dear Friends and Colleagues,
Without belaboring the point, while I find this an interesting
suggestion,
it would imply that I should place [Company] in the subject line
because I
work for a company. All rules have their excess, and I would submit
that J
Paul (actually Steve) and company have done a great job maintaining
the
integrity of this list. If an occasional message is allowed thru that
has
potential commercial implications, I would rather have that than
complete
(or no) censorship. If individuals wish to express their displeasure
at a
specific posting (irrespective of the source) this allows discussion,
and
if necessary, re-calibration of the gating algorithm. And regarding
vendors monitoring the list versus actively soliciting messages, I
suspect
that most monitor this list to gather useful information, much of
potential use to their customers. But this is quite different from
"open
access" for solicitation.

1 week ago
While I'm here, some additional comments:
1.) Cost of the ISAC meeting. I find the registration of $650 (US) a
bit
steep for a meeting. While I was on ISAC Council, a resolution was
passed
by Council that the Congress be shortened (in an attempt to reduce
the
registration fee and overall cost) and be held annually. The
combination
of a $650 registration fee plus hotel costs (esp in Europe- for US
scientists) makes it difficult for many labs to bring key technicians
and
post-docs; it even makes it difficult for many senior scientists to
justify. To put this in a bit of perspective, the American Asc for
Cancer
Research charges a $425 (US) registration fee to their members for
their 4
day. An important difference is that the AACR has SIGNIFICANT
industrial
support. We need to do as much as possible to make our meeting's
overall
cost "reasonable". ISAC must do everything it can to keep the overall
meeting costs (registration, airfare, hotels and meals) to a minimum
(this
ain't ASCO).

1 week ago
2.) Vendor support. This is essential for ISAC or any other
professional
society. At one point, I was chair of the committee that raised
donations
for the ISAC Congress, and approached every company that I could think
of
that sold products to our membership quite shamelessly. Every society
does
this because it helps lower the cost of the meeting (or should) for
the
individual attendees. And by the way, the money the vendors use comes
from
the customers. Donations are thus a means to widen the available
attendees
who will buy their products. The approach must be balanced.

1 week ago
3) Meeting location. While I appreciate this is a problematic issue,
refer
back to item "1. At one point, ISAC restricted the Congress to
"smaller"
venues to keep us together. Much science gets discussed (in places
that
tend to serve alcohol) at ISAC, and that's one reason I attend
(discussion, not libations). Where will we gather in Budapest (after
poster sessions)? Unlikely to be the InterContinental Hotel - I
probably
could not afford one beer there.
4) What is the focus of ISAC? I'm running for Clinical Councilor
(sorry, I
too had to get in my pitch), so my potential constituency for the
most
part has a limited focus. But the society needs to have a balanced
focus
on flow and image cytometry. And I don't think that DNA, RNA or
protein
arrays should be our soup!. Our unit of measurement is the cell, and
once
you bust it up, someone with better technical expertise should be
telling
scientists and clinicians what best to do. That said, we should
communicate and work with "non-cellular

1 week ago
ientists where our
interests
and technologies intersect.
I need to go home for the weekend. A Happy Easter to all.
Sincerely,

Vince

p.s. I'm running for Clinical Councilor. If you live in Chicago vote
early
and often

T. Vincent Shankey, Ph.D.
Advanced Technology Center
Beckman Coulter, Inc.
vinc...@coulter.com
(305)-380-2430

2 days ago
Purdue Cytometry Mailing List: Re: Nonreadable FCS 3 files from
From : J. Paul Robinson <j...@flowcyt.cyto.purdue.edu> ... For data
analysis we want to > use a centraly designed templates made in Flow
Jo software. ...
www.cyto.purdue.edu/hmarchiv/2004/0997... - 8k - Cached - Similar
pages - Note this

1 day ago

Useful Links & Resources - Flow Cytometry Site
General. Purdue University Cytometry Labs (J. Paul Robinson) ...
Software. FlowJo. http://flowjo.com/. FACSPress and FACSAssistant.
http://www.fcspress.com/ ...
idi.harvard.edu/content/facilities/cyt...
- 42k - Cached - Similar pages - Note this

2 days ago
FCSPress Training Sessions?
From: Ray Hicks (r...@fcspress.com)
Date: Thu Oct 11 2001 - 19:42:36 EST
In reply to: Jennifer Wilshire: "FlowJo Training Sessions (Midwest)"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
--------------------------------------...
Most of you will find FCSPress so easy to use that you won't need
training
sessions. You can download a copy to try it out for yourselves at
http://www.fcspress.com/html/FCSPArea.ht...
where you'll also find documentation and details of a special offer.
If you find you DO need some assistance using FCSPress, I'm planning
on
staying in Cambridge for quite some time, and I'd be happy to answer
any of
your queries via e-mail at supp...@fcspress.com
Cheers,
Ray
--
e-mail mailto:R...@FCSPress.com
Web http://www.FCSPress.com
Tel +44 797 453 8647
+44 12

2 days ago
Purdue Cytometry Mailing List: RE: Diva 4.0 files ... Mark E.
Munson Sales Manager Verity Software House, Inc. 45A Augusta Road PO
Box 247 Topsham, ME 04086 Phone: 207-729-6767 x191 Fax:
207-729-5443 ...http://www.cyto.purdue.edu/hmarchiv/2004... 6.2KB
74%

2 days ago
FlowJo Training Sessions (Midwest)

From: Jennifer Wilshire (GoFlowJo@yahoo.com)
Date: Thu Oct 11 2001 - 06:20:11 EST

* Next message: Richard Grenfell: "Multiple plate loader?"
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* Next in thread: Ray Hicks: "FCSPress Training Sessions?"
* Reply: Ray Hicks: "FCSPress Training Sessions?"
* Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]

I will be in Wisconsin, Minnesota, and Iowa offering FlowJo seminars
and training sessions next week. All of the hosting sites have
graciously opened the sessions to members of the Cytometry list. Times
and locations are listed below. Thanks, Jennifer Wilshire FLOWJO
SEMINARS ______________________________________ Monday, October 15th
University Hospitals Clinical Sciences Center 600 Highland Ave,
Madison WI Room K4/418 10:00 AM ______________________________________
Tuesday,

2 days ago
October 16th University of Minnesota Cancer Center 425 E. River Road
Room 450 9:00 AM ­ Introduction to FlowJo 10:30 AM ­ Advanced Topics
_______________________________________ Tuesday, October 16th
University of Minnesota, St. Paul Building AS/VM Room 295M 2:00 PM
_______________________________________ Friday, October 19th
University of Iowa South East 310 General Hospital Bean Conference
Room 10:00 AM _______________________________________ If you are
interested in hosting a FlowJo seminar, please let me know. Upcoming
trips are in the planning stages for: -NJ, PA, DC -Florida. -Boston
(home) Jennifer Wilshire, Ph.D. Application Scientist
GoFlowJo@Yahoo.com Tree Star, Inc. www.flowjo.com

2 days ago
Recent FlowJo announcement
From: Steve Kelley (SKELLEY@flowcyt.cyto.purdue.edu)
Date: Wed Dec 30 1998 - 06:13:04 EST

* Next message: Steve Kelley: "Possible minor disruption"
* Previous message: Mark A. Corio: "Chemdex no help..."
* Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]

I know that many people on the mailing list are adamantly opposed to
anything that even looks a little commercial, and to

try to forestall any possible complaints about the FlowJo
announcement, I'll explain what I'd like to see.

In my opinion, announcements about new products, and product updates
are completely appropriate as long as they aren't abused

. I've never wanted to specifically encourage that, because I really
don't want to be put into a position of having to decide whether a
message is an "

announcement"

or an "

advertisement".

2 days ago
The companies involved in our cytometry community have always been
extremely good 'citizens' as far as I can tell.

We have representatives of many companies on the list, and they have
always had the power to make my life miserable, and the list no more
than

junk mail.

Instead, they have helped build this mailing list into one of the most
useful around, through their contributions and responses. oI'm not
going to try to set specific rules about what people can say about
their wn products, and when they can say it. I'll just ask that
everyone continue to show restraint; that the commercial
representatives ask themselves before they submit an announcement
whether they'd mind seeing every other company in the business sending
the same message they are about to, and that the

non- commercial (and anti-commercial) people accept discreet
announcements as simple information,

2 days ago
and continue the sometimes brisk discussions about problems and
benefits of particular products, alongside the purely scientific (and
occasionally purely entertaining) conversation. Steve Steve Kelley

kelley@flowcyt.cyto.purdue.edu

Purdue University

Cytometry Laboratories

(765) 494-0757

-- voice B050 Hansen LSRB,

Purdue University

(765) 494-0517

-- fax

West Lafayette, Indiana, 47907

-- End --

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* Previous message: Mark A. Corio: "Chemdex no help..."
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2 days ago
Purdue Cytometry Mailing List: RE: Software for merging FSC 3 fi
... Purdue Cytometry Mailing List: RE: Software for merging FSC ...
J.
Herbert Technical Support Manager Verity Software House, Inc. PO Box
247 ...http://www.cyto.purdue.edu/hmarchiv/2005... 6.6KB
73%

2 days ago
Purdue Cytometry Mailing List: Announcing User Forum at
Verity ...
Verity Software House is pleased to announce the creation of a Verity
User's Forum on our ...
http://www.cyto.purdue.edu/hmarchiv/2002... - 4.6KB
78%

2 days ago
Purdue Cytometry Mailing List: Re: Log-like transforms
... Purdue Cytometry Mailing List: Re: Log-like transforms ...
purposes. > > > > Bruce > > > > C. Bruce Bagwell MD, Ph.D. > >
President > > Verity Software House > > 45A Augusta Road > > PO Box
247 > > Topsham, ME 04086 ...
http://www.cyto.purdue.edu/hmarchiv/2006... - 10.0KB

2 days ago
Purdue Cytometry Mailing List: RE: utility software to convert
fcs1.0 to fcs2.0 ... Since you are using the PC, I suggest you
contact
Verity Software (207-729-6767) . I quickly tried this function this
AM ...
http://www.cyto.purdue.edu/hmarchiv/1998... - 7.8KB
73%
Mitch Haynes - 23 May 2008 22:56 GMT
> This message: [ Message body ] [ More options ]
>
[quoted text clipped - 1268 lines]
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> - Show quoted text -

Flow Image and CYTOMETRY SOFTWARE

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