commercial announcements
From: Marcia Woda (Marcia.W...@banyan.ummed.edu)
Date: Wed Dec 30 1998 - 15:23:58 EST
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________________________________________
Why couldn't commercial parties make a short statement via this list
to
direct interested viewers to their company's Web site to look at new
products, updates, etc.
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Latest FlowJo focuses on presentation graphics
From: Adam Treister (a...@treestar.com)
Date: Tue Dec 29 1998 - 10:34:17 EST
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________________________________________
There's a new version of FlowJo now available on our web site.  Since
its
initial public release eighteen months ago, FlowJo has had the
reputation
that it is very powerful in its batch processing, but didn't have
enough
page layout capabilities to create publication quality reports
without
an
intermediate drawing program. This release adds all those
capabilities.

We've added histogram overlays, colored dot plots, more and more
flexible
statistics, a palette of annotation tools, and a custom print preview
window to position as many graphs on a page as you wish, without
fighting
the printer's page breaks.  Or if you don't want to print to paper,
we'll
produce slide shows, animations or web pages directly from FlowJo's
layout
editor.

In addition, we've added a DNA / Cell Cycle platform to our Kinetics,
Compensation and Calibration tools.  This new platform will fit
multiple
models to the histogram, and let you tailor the models' constraints
interactively.  Because its built into FlowJo, you can easily combine
these
analyses with other phenotyping and batch analyses.

We encourage you to go take a look at:  http://www.treestar.com/flowjo
For the full list of features:
http://www.treestar.com/flowjo/html/version.html

Adam Treister

-----------------------------
Adam Treister
a...@treestar.com
http://www.treestar.com 800-366-6045
-----------------------------
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Possible minor disruption
From: Steve Kelley (SKEL...@flowcyt.cyto.purdue.edu)
Date: Wed Dec 30 1998 - 06:24:59 EST
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Our lab is in the process of making a very significant networking
change.

Sometime over the next week or two (I can't yet predict exactly when)
our main
server, flowcyt.cyto.purdue.edu, will be getting a new internet
address.  I will be
working very closely with our campus networking group, who are
extremely
good, and I don't think there will be any significant disruption,
however, we must
always be prepared.

The most likely effect will be a very short time period (probably no
more than 10
minutes) when the web and ftp site will be unavailable.

Due to the way names and addresses are matched up on the Internet,
it's also
possible that some people will have temporary trouble sending mail to
us and
connecting to our web site once it's been changed and is running
correctly.
This would be due to the address change propagating through the
worldwide
system, and should not in any event take longer than two days.

If all goes as our networking people expect, that phase should take
no
more
than one hour, rather than a couple of days, but I wanted to give
everyone a
little warning of what's going on.

I will send a reminder shortly before we make the change to flowcyt,
and make
sure I give out  an alternate email address in case you have
unexpected
problems.

None of the names of our servers will change, they will still be
       flowcyt.cyto.purdue.edu
       www.cyto.purdue.edu and
       ftp.cyto.purdue.edu

The only change will be their internet address.

Steve

Steve Kelley            kel...@flowcyt.cyto.purdue.edu

Purdue University Cytometry Laboratories    (765) 494-0757  -- voice
B050 Hansen LSRB, Purdue University         (765) 494-0517  -- fax
West Lafayette, Indiana, 47907

-- End --
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Latest FlowJo focuses on presentation graphics
From: Adam Treister (a...@treestar.com)
Date: Tue Dec 29 1998 - 10:34:17 EST
*       Next message: Douglas Dooley: "Sort purity"
*       Previous message: Duncan Greig: "counting yeast cells"
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________________________________________
There's a new version of FlowJo now available on our web site.  Since
its
initial public release eighteen months ago, FlowJo has had the
reputation
that it is very powerful in its batch processing, but didn't have
enough
page layout capabilities to create publication quality reports
without
an
intermediate drawing program. This release adds all those
capabilities.

We've added histogram overlays, colored dot plots, more and more
flexible
statistics, a palette of annotation tools, and a custom print preview
window to position as many graphs on a page as you wish, without
fighting
the printer's page breaks.  Or if you don't want to print to paper,
we'll
produce slide shows, animations or web pages directly from FlowJo's
layout
editor.

In addition, we've added a DNA / Cell Cycle platform to our Kinetics,
Compensation and Calibration tools.  This new platform will fit
multiple
models to the histogram, and let you tailor the models' constraints
interactively.  Because its built into FlowJo, you can easily combine
these
analyses with other phenotyping and batch analyses.

We encourage you to go take a look at:  http://www.treestar.com/flowjo
For the full list of features:
http://www.treestar.com/flowjo/html/version.html

Adam Treister

-----------------------------
Adam Treister
a...@treestar.com
http://www.treestar.com 800-366-6045
-----------------------------
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Re: CellQuest
From: Joe Trotter (trot...@scripps.edu)
Date: Thu Jul 02 1998 - 17:00:40 EST
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Dear Yousaf,

       WinMDI reads CellQuest data files because they are in FCS2.0
format. The header provides the byte offsets for the TEXT portion of
the
file and the DATA portion of the file, so it is rather straight
forward to read a FCS datafile in a platform independent manner.
For more info see the FCS documentation at

       http://nucleus.immunol.washington.edu/ISAC.html

If you're asking about getting Mac files to a PC running Windows,
that is a different issue. Networks make it easy, just be sure
the files are transferred in a BINARY mode. Without a network, most
folks find using a PC formatted floppy or other device and utilizing
the
Mac's ability to read a foreign disk via PC Exchange, for example,  a
good solution. WinMDI doesn't care once the files are available to
the PC on a readable device (floppy, ZIP, CDROM, etc...).

                       Regards,

                               Joe

On Sun, 29 Jun 2098, Ed Yousaf wrote:

Joe Trotter
Director, Flow Cytometry
Mailstop Imm-20
The Scripps Research Institute
10666 North Torrey Pines Rd.
La Jolla, California 92037
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004 - 17:35:29 EST
Year 2000 Compliance: ALL FLOW-ERS AFFECTED!
From: Mario Roederer (Roede...@Beadle.Stanford.edu)
Date: Tue May 26 1998 - 12:48:49 EST
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ERS
AFFECTED!"
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AFFECTED!"
*       Reply: Ray Hicks: "Re: Year 2000 Compliance: ALL FLOW-ERS
AFFECTED!"
*       Maybe reply: Michael Cosgrove: "Re[2]: Year 2000 Compliance:
ALL
FLOW-ERS AFFECTED!"
*       Reply: Hazel Davey: "Re: Year 2000 Compliance: ALL FLOW-ERS
AFFECTED!"
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ALL
FLOW-ERS AFFECTED!"
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________________________________________
There is a small hitch that even BDIS has apparently forgotten (given
the copy
of their missive posted to the mailing list)...  The FCS2.0 standard
is NOT year
2000 compliant.  Therefore, all software that produces FCS2.0 data,
and this
currently means every manufacturer out there, has the year 2000 bug.

However, the current FCS3.0 standard, which is only a little
different
from
FCS2.0, does not have this bug.

This means that EVERYONE will have to upgrade acquisition software in
the next
18 months.

I sincerely hope that BDIS, Coulter, and the other manufacturers take
it upon
themselves to show good grace to our community by providing this
MINOR
software
upgrade free of charge.

I'm sure we'd all love to hear from them on this issue!  I challenge
each of
them to be the first to announce this free upgrade, and score major
brownie
points.  (Flower points?)

mr
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AFFECTED!"
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AFFECTED!"
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AFFECTED!"
*       Maybe reply: Michael Cosgrove: "Re[2]: Year 2000 Compliance:
ALL
FLOW-ERS AFFECTED!"
*       Reply: Hazel Davey: "Re: Year 2000 Compliance: ALL FLOW-ERS
AFFECTED!"
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ALL
FLOW-ERS AFFECTED!"
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Mean or geometric mean
From: Joe Trotter (trot...@scripps.edu)
Date: Wed Mar 25 1998 - 20:35:38 EST
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________________________________________
Wolfgang and Marcus,

   It seems reasonable to jump in here a bit.... My comments were in
answer to
how WinMDI calculates numbers, and not about the appropriateness of
one
statistic
verses another.

   Most everyone agrees that for most log compressed data it is not
a
good idea to use the
arithmetic mean. Some prefer the median and others the geometric
mean,
both of which have can have their uses (as pointed out by Ray Hicks
on
March 19).
For example, I would prefer to use the median during acquisition when
checking
how well two log channels may be compensated, many users over-
compensate
when
using only dotplot displays during setup and the median is a very
useful
statistic in verifying
that the compensated "background" is the same as the isotype control.
In
this case, the
piling up that can occur on the low end is best dealt with by the
median. On the other
hand, the best "average" fluoresecence of a log distribution within a
region is probably
going to be the geometric mean.

  WinMDI provides median, geometric mean, and arithmetic mean output
for histogram
displays, but has no median output for bivariate displays. It is one
of
those things on
the list of TODO items.... soon.

       Regards,

       Joe
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