Re: What is a dump channel?From: Randy T. Fischer
[fischer1@mail.nih.gov]
Sent: Friday, August 12, 2005 9:26 AM
To: Cytometry Mailing List
Subject: Re: What is a dump channel?
Hi Guy,

At least for our lab, we use a �dump� channel for exactly what it
sounds like- a �trash dump�.  We are interested primarily in human B
cells, and in some of our sick patients these can be quite rare in
comparison to the other types of peripheral blood cells.  So, to be
able to more easily analyze these rare populations, we label T-cells,
NK-cells, monocytes, dendritic cells and dead cells with a single
fluorochrome/color and anything positive in this channel is �dumped�
out of the populations to be analyzed.

Randy T. Fischer, NIH/NIAMS
B Cell Biology Group
9000 Rockville Pike
Bldg 10, Room 6D50
Bethesda, MD 20892
(301) 594-3537 (voice)
(301) 402-2209 (fax)

--------------------------------------------------------------------------------
From: Guy Hermans <Guy.Hermans@ablynx.com>
Date: Thu, 11 Aug 2005 03:17:07 -0400
To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
Subject: What is a dump channel?

Hi all,

After doing quite some reading on the list and in publications, I got
the impression various people use the term "dump channel" in reference
to different concepts/uses. Could some of the gurus out there clarify
this one for me and the rest of us out there?

thanks,

Guy

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