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Medical Forum / Diseases and Disorders / AIDS / February 2008PURDUE CYTOMETREY MAIL LIST DNA ANLYSIS SOFTWARE DENOVOSOFTWARE 2007
RE: DNA analysis software > * This message: [ Message body ] [ More options ] > * Related messages: [ Next message ] [ Previous message ] [ In > reply to ] [ Next in thread ] > From: Novo, David <david.n...@denovosoftware.com> > Date: Fri Feb 16 2007 - 19:05:22 EST > Hello Ibtissam, > Multicycle is a very commonly used DNA analysis program. It can > automatically detect the number of aneuploid populations that you > have > (if any) and runs them through several different models with > different > fitting parameters to help you determine which is the best one for > your > data. It also has a wide range of debris and aggregate compensation > to > allow it to be used with a wide variety of sample preparation > methods. > Multicycle has just been incorporated as a plug in module to FCS > Express, so that you get the benefits of the high end DNA analysis as > well as the ease of use and gating/presentation abilities of FCS > Express. It really makes DNA analysis a snap. > There is some information available athttp://www.denovosoftware.com/site/Multicycleplugin.shtml > -Dave > ----------------------------------- > David Novo > President > De Novo Software > david.n...@denovosoftware.com > RE: DNA analysis software > [quoted text clipped - 26 lines] > > De Novo Software > > david.n...@denovosoftware.com More options Jan 17, 1:52 pm Newsgroups: misc.health.aids From: Mitch Haynes <mitchhay...@gmail.com> Date: Thu, 17 Jan 2008 11:52:56 -0800 (PST) Local: Thurs, Jan 17 2008 1:52 pm Subject: PURDUE CYTOMETRY DNA DELETE AFTER YOU READ PURDUE CYTOMETRY MAIL LIST Reply | Reply to author | Forward | Print | View thread | Show original | Report this message | Find messages by this author Verity House software RE: gate-specific data cropping * This message: [ Message body ] [ More options ] * Related messages: [ Next message ] [ Previous message ] [ Maybe in reply to ] From: VSH Tech Support <T...@vsh.com> Date: Fri Feb 16 2007 - 09:01:56 EST Stevan At the risk of sounding like a commercial, WinList can do exactly what you want. The problem of "sucking up air" or perhaps having a clogged nozzle at the end of a run has gotten all of us at one time or another, and then the data (most of which is probably fine) appears to be unusable. WinList addresses this issue with the FTIM parameter, which is basically a "chronology" parameter defined over 1024 channels. Each event is assigned an FTIM channel based on its order in the listmode file, channel zero having the first n/1024 events, and channel 1023 having the last n/1024 events. Displaying a 2P dot plot of FTIM vs. side scatter, for example, will clearly show the aberrant events. You may then gate on the "good" events, or gate out the "bad" events. Either way, you can salvage an otherwise bad run. You can also save the gated listmode using WinList's Save Data Source option, and it will contain only the "good" events. Another feature of the Save Data Source option is the ability to set the number of events to save in the listmode file, which at least partially addresses the desire to save a larger file into smaller segments that will each have the same number of events within given regions, as you are requesting. Best regards, Don Donald J. Herbert Technical Support Manager Verity Software House -----Original Message----- From: Stevan Lauriault [mailto:ste...@lauriault.com] Sent: Friday, February 09, 2007 6:24 PM To: cyto-inbox Subject: Re: gate-specific data cropping Thanks for your very helpful comments. I guess what I am trying to get at is this: is there any analysis software available that can reduce list mode data in a reverse order-specific matter (in reverse sequence)? Correct me if I'm wrong, but I believe this function would also help in the event that a user lets a sample run dry and sucks up air bubbles, or over collects their intended gating target (for example, in acquisition and storage, 5000 of R1). Thanks, Stevan ---------- Original Message ---------------------------------- From: "Byron Ellis" <bcel...@stanford.edu> Date: Wed, 7 Feb 2007 16:21:28 -0800 >On 2/7/07, Stevan Lauriault <ste...@lauriault.com> wrote: >> Hi and thanks, >> Since we want to directly compare MFIs (in FL2) between any two >> subsets (R1 vs. R3) >that are isolated on a bivariate dot plot (FL1 vs. FL4), doing so in >separate sample tubes would introduce the extra probability of >experimental error between sample tubes. >Directly comparing the subsets from the same sample tube, and even >better, the same data set, would eliminate this extra unknown. >It doesn't necessarily eliminate the unknown, it may just keep you from >finding out about it. If you were to prepare one sample on Monday, see [quoted text clipped - 4 lines] >surprised if that actually happened in your case? Yes. >Would I do replicates anyway? Yes. >> Let us say we have acquired 100,000 total events (stained with 3 >> fluorochromes, [quoted text clipped - 4 lines] >Within those 100,000 total events, there are 500 of R1, 700 of R2 and >1200 of R3. >> If we crop the 100,000 total events to 75,000 total events (from the >> top in >stack-collected data), there will become exactly 500 events in the R2 region. If we crop >the 100,000 total events to 40,000 total events, there will become 500 events in R3. >> Now the three populations; R1, R2, and R3 each have exactly 500 total >> events, and we >can directly compare MFIs among the subsets that have identical sample sizes. We could >also then say that, when comparing means between these subsets under >identical experimental conditions, Rx gives a consistently higher fluorescent signal than Ry. >Well, depending on what you're doing (you've never said how you intend >to compare means), equal sample sizes is not particularly required so [quoted text clipped - 4 lines] >one-sided t-tests essentially, that give you directionality statements >such as mu_{R_1} > mu_{R_2} ). In this case your assumptions are: >1. Each group is independent >2. Normality of group populations (or at least "close enough" to >Normal) 3. The _population_ variances of the dependent variable for >each group are equal (or pretty close). (Actually check this. It would >be unsurprising to encounter large differences in the population >variances among your different groups). >Like I say, it depends a bit on what you intend to do for your >analysis. For example, _two_-way ANOVA actually expects equal sample >sizes. >> Is this a reasonable strategy? Is there any software available that >> can perform this >function? >On an FCS file directly? Probably not (neither the manipulation of the >flow data nor the testing). Once you've got the data out of FCS form >and appropriately labeled with group membership or split into separate >files or something similar, then any reasonable statistics package >(Excel is not included in the list of reasonable statistics packages) >can perform the statistical tests. >Personally, I use R (http://www.r-project.org) for analyzing all the >flow data I have, but it can be intimidating for new users (though [quoted text clipped - 8 lines] >expect to be available in the next Bioconductor release (probably >sometime in April). >Hope that helps, >Byron >> Kind Regards, >> Stevan Lauriault >> ---------- Original Message ---------------------------------- >> From: "Byron Ellis" <bcel...@stanford.edu> >> Date: Tue, 6 Feb 2007 12:17:35 -0800 >> >Speaking as a statistician, I would not count running the same tube >> >3 times or simply cutting the FCS file (the two are equivalent) as [quoted text clipped - 5 lines] >> >biological variability of the cells and a single sample preparation >> >only captures the latter. >> >On 2/5/07, Stevan Lauriault <ste...@lauriault.com> wrote: >> >> Dear All, >> >> Question: >> >> We are isolating leukocyte subsets to measure and compare relative >> >> levels of [quoted text clipped - 5 lines] >> >> statistically analyze the relative mean fluorescence intensities >> >> of a biomarker, among the subsets. >> >> To get statistically comparable sample sizes among these subsets, >> >> we are running the >same >> >> tube three times and changing the storage criteria to 500 events [quoted text clipped - 9 lines] >> >> events, three times to get an identical sample size for all three >> >> gated subsets (R1, >R2, >> >> and R3). >> >> Scientifically, this shouldn't be a problem, since flow cytometry >> >> data is collected randomly within the same sample tube. Not only [quoted text clipped - 6 lines] >> >> scientists might be uneasy with the idea of manipulating raw >> >> list-mode data. >> >> However, Since the data is collected randomly from the same >> >> sample, doing this [quoted text clipped - 3 lines] >only >> >> be cropping (from stack-collected data) the most recent events collected. >> >> Currently, we are acquiring 3 different data files for each tube, >> >> and adjusting the acquisition and storage settings for each >> >> acquisition. Is there a way to perform a gate-specific data >> >> cropping function with any current flow cytometry data analysis software? >> >> Kind Regards, >> >> Stevan Lauriault >> >> ________________________________________________________________ >> >> Sent via the WebMail system at lauriault.com >> >-- >> >Byron Ellis (byron.el...@gmail.com) >> >"Oook" -- The Librarian >> ________________________________________________________________ >> Sent via the WebMail system at lauriault.com >-- >Byron Ellis (byron.el...@gmail.com) >"Oook" -- The Librarian ________________________________________________________________ Sent via the WebMail system at lauriault.com Received on Fri Feb 16 16:38:00 2007 * This message: [ Message body ] * Next message: Mara.Roc...@moredun.ac.uk: "ovine T regs" * Previous message: Atwater, Susan: "RE: Reporting clinical results" * Maybe in reply to: Stevan Lauriault: "gate-specific data cropping" * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 E RE: DNA analysis software * This message: [ Message body ] [ More options ] * Related messages: [ Next message ] [ Previous message ] [ In reply to ] From: VSH Tech Support <T...@vsh.com> Date: Wed Feb 21 2007 - 11:29:45 EST Hello Ibtissam, ModFit LT, for PC or Mac, has advanced modeling capability for research applications in DNA cell cycle analysis. You may use any of the model templates the program offers, or create your own models for non- traditional analysis, including non-mammalian DNA cell cycle studies. ModFit LT can be linked to our WinList program to provide a complete cell cycle analysis on any number of sub-populations with a single click of a button. For an overview, visit <http://www.vsh.com/products> http://www.vsh.com/products . Best regards, Don Donald J. Herbert Technical Support Manager Verity Software House ________________________________ From: Ibtissam Abdul-Jabbar [mailto:iajab...@cicr.uq.edu.au] Sent: Wednesday, February 14, 2007 11:41 PM To: cyto-inbox Subject: DNA analysis software Dear All, before buying software to analyse DNA on PC, I would like to get your opinion. I already have ModFit for Macintosh. What are you using and what do you recommend for research purposes. Is MultiCycle Av the one of choice? I appreciate your comments. Ibtissam A Jabbar (PhD) Manager of the FACS facilities Diamantina Institute for Cancer, Immunology and Metabolic Medicine (DI) The University of Queensland Level 4 R Wing Princess Alexandra Hospital Ipswich Rd Buranda QLD 4102 Australia Ph: 07 3240 5945 Fax: 07 3240 5946 Mob: 0401154744 Received on Thu Feb 22 15:18:00 2007 * This message: [ Message body ] * Next message: Uriel TK: "Re: Ratio Measurement of Mitochondrial Membrane Potential with FACSDiva" * Previous message: Howard Shapiro: "Re: Ratio Measurement of Mitochondrial Membrane Potential with FACSDiva" * In reply to: Ibtissam Abdul-Jabbar: "DNA analysis software" * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST Re: gate-specific data cropping * This message: [ Message body ] [ More options ] * Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] From: WEHICytometry <facs_c...@wehi.EDU.AU> Date: Mon Feb 12 2007 - 18:25:38 EST Stevan, For what it's worth, we have a utility we call Hackit that can crop cells from the front or back of a FCS file ( see http:// www.wehi.edu.au/cytometry/freesoftware/index.html). We use it for the tasks you suggest in cleaning up violated data. I don't know how useful that would be for your current purpose because it can't do gating; numbers clipped are total cell numbers. I guess if you knew the proportions of your gated cells you could eventually clip out the file segments you need. Frank Battye. On 10/02/2007, at 10:24 AM, Stevan Lauriault wrote: > Thanks for your very helpful comments. I guess what I am trying to > get at is this: is [quoted text clipped - 7 lines] > example, in acquisition and > storage, 5000 of R1). | | << The Cytometry Laboratory \__/ <<<< The Walter & Eliza Hall Institute ------!!<<<<<< 1G Royal Parade, Parkville /!!\ <<<< Victoria 3050, Australia o !! \ << ph: 61_3_9345 2540, fax: 61_3_9347 0852 Received on Tue Feb 13 15:18:00 2007 * This message: [ Message body ] * Next message: Uriel TK: "Re: early apoptotic cells" * Previous message: Darzynkiewicz, Zbigniew: "RE: early apoptotic cells" * In reply to: Stevan Lauriault: "Re: gate-specific data cropping" * Next in thread: VSH Tech Support: "RE: gate-specific data cropping" * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST Re: gate-specific data cropping * This message: [ Message body ] [ More options ] * Related messages: [ Next message ] [ Previous message ] [ Maybe in reply to ] [ Next in thread ] [ Replies ] From: Stevan Lauriault <ste...@lauriault.com> Date: Fri Feb 09 2007 - 18:24:07 EST Thanks for your very helpful comments. I guess what I am trying to get at is this: is there any analysis software available that can reduce list mode data in a reverse order-specific matter (in reverse sequence)? Correct me if I'm wrong, but I believe this function would also help in the event that a user lets a sample run dry and sucks up air bubbles, or over collects their intended gating target (for example, in acquisition and storage, 5000 of R1). Thanks, Stevan ---------- Original Message ---------------------------------- From: "Byron Ellis" <bcel...@stanford.edu> Date: Wed, 7 Feb 2007 16:21:28 -0800 >On 2/7/07, Stevan Lauriault <ste...@lauriault.com> wrote: >> Hi and thanks, >> Since we want to directly compare MFIs (in FL2) between any two subsets (R1 vs. R3) >that are isolated on a bivariate dot plot (FL1 vs. FL4), doing so in separate sample >tubes would introduce the extra probability of experimental error between sample tubes. >Directly comparing the subsets from the same sample tube, and even better, the same data >set, would eliminate this extra unknown. >It doesn't necessarily eliminate the unknown, it may just keep you >from finding out about it. If you were to prepare one sample on [quoted text clipped - 4 lines] >would I be surprised if that actually happened in your case? Yes. >Would I do replicates anyway? Yes. >> Let us say we have acquired 100,000 total events (stained with 3 fluorochromes, >measured in FL1, FL2, and FL4 respectively). We are using a bivariate dot plot of FL1 >vs. FL4 in which we are isolating 3 subsets (R1, R2, and R3) based on their relative >expressions of FL1 vs. FL4. We then want to compare the MFIs, as measured in FL2, among >these subsets. Within those 100,000 total events, there are 500 of R1, 700 of R2 and >1200 of R3. >> If we crop the 100,000 total events to 75,000 total events (from the top in >stack-collected data), there will become exactly 500 events in the R2 region. If we crop >the 100,000 total events to 40,000 total events, there will become 500 events in R3. >> Now the three populations; R1, R2, and R3 each have exactly 500 total events, and we >can directly compare MFIs among the subsets that have identical sample sizes. We could >also then say that, when comparing means between these subsets under identical >experimental conditions, Rx gives a consistently higher fluorescent signal than Ry. >Well, depending on what you're doing (you've never said how you intend >to compare means), equal sample sizes is not particularly required so [quoted text clipped - 4 lines] >one-sided t-tests essentially, that give you directionality statements >such as mu_{R_1} > mu_{R_2} ). In this case your assumptions are: >1. Each group is independent >2. Normality of group populations (or at least "close enough" to Normal) >3. The _population_ variances of the dependent variable for each group >are equal (or pretty close). (Actually check this. It would be >unsurprising to encounter large differences in the population >variances among your different groups). >Like I say, it depends a bit on what you intend to do for your >analysis. For example, _two_-way ANOVA actually expects equal sample >sizes. >> Is this a reasonable strategy? Is there any software available that can perform this >function? >On an FCS file directly? Probably not (neither the manipulation of the >flow data nor the testing). Once you've got the data out of FCS form >and appropriately labeled with group membership or split into separate >files or something similar, then any reasonable statistics package >(Excel is not included in the list of reasonable statistics packages) >can perform the statistical tests. >Personally, I use R (http://www.r-project.org) for analyzing all the >flow data I have, but it can be intimidating for new users (though [quoted text clipped - 8 lines] >expect to be available in the next Bioconductor release (probably >sometime in April). >Hope that helps, >Byron >> Kind Regards, >> Stevan Lauriault >> ---------- Original Message ---------------------------------- >> From: "Byron Ellis" <bcel...@stanford.edu> >> Date: Tue, 6 Feb 2007 12:17:35 -0800 >> >Speaking as a statistician, I would not count running the same tube 3 >> >times or simply cutting the FCS file (the two are equivalent) as three [quoted text clipped - 4 lines] >> >due to experimental error as well as the biological variability of the >> >cells and a single sample preparation only captures the latter. >> >On 2/5/07, Stevan Lauriault <ste...@lauriault.com> wrote: >> >> Dear All, >> >> Question: >> >> We are isolating leukocyte subsets to measure and compare relative levels of >expression >> >> of certain antigens. For example, there are three subsets within a bivariate dot >plot; >> >> gated on R1, R2 and R3 respectively, and we would like to statistically analyze the >> >> relative mean fluorescence intensities of a biomarker, among the subsets. >> >> To get statistically comparable sample sizes among these subsets, we are running the >same >> >> tube three times and changing the storage criteria to 500 events for each subset [quoted text clipped - 5 lines] >total >> >> events, three times to get an identical sample size for all three gated subsets (R1, >R2, >> >> and R3). >> >> Scientifically, this shouldn't be a problem, since flow cytometry data is collected >> >> randomly within the same sample tube. Not only could we run one acquisition to get [quoted text clipped - 3 lines] >some >> >> scientists might be uneasy with the idea of manipulating raw list-mode data. >> >> However, Since the data is collected randomly from the same sample, doing this >should >> >> give exactly the same readings as if we just collected less events, since we would >only >> >> be cropping (from stack-collected data) the most recent events collected. >> >> Currently, we are acquiring 3 different data files for each tube, and adjusting the >> >> acquisition and storage settings for each acquisition. Is there a way to perform a >> >> gate-specific data cropping function with any current flow cytometry data analysis >> >> software? >> >> Kind Regards, >> >> Stevan Lauriault >> >> ________________________________________________________________ >> >> Sent via the WebMail system at lauriault.com >> >-- >> >Byron Ellis (byron.el...@gmail.com) >> >"Oook" -- The Librarian >> ________________________________________________________________ >> Sent via the WebMail system at lauriault.com >-- >Byron Ellis (byron.el...@gmail.com) >"Oook" -- The Librarian ________________________________________________________________ Sent via the WebMail system at lauriault.com Received on Mon Feb 12 12:18:00 2007 * This message: [ Message body ] * Next message: Dan Smith: "Interferon alpha & Interferon Beta intracellular staining" * Previous message: RAJA ALAMELU: "[ seeking K562 cell line ]" * Maybe in reply to: Stevan Lauriault: "gate-specific data cropping" * Next in thread: WEHICytometry: "Re: gate-specific data cropping" * Reply: WEHICytometry: "Re: gate-specific data cropping" * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST RE: gate-specific data cropping * This message: [ Message body ] [ More options ] * Related messages: [ Next message ] [ Previous message ] [ Maybe in reply to ] [ Next in thread ] From: Nebe-Von-Caron, G <g.nebe-von-ca...@unipath.com> Date: Thu Feb 08 2007 - 09:36:37 EST The number of events collected will not be the influential thing as with a minimum of 500 events you get already a damn good estimate of your MFI. Just as an educational exercise you should do is to look at MFI estimate of one population over events. It should indicate that your estimate if the sample mean after 100 "samples" (each cell being a sample on its own) is already pretty good. You might want to look at the distribution of mean fluorescence or more important of single event residuals (distance from mean) over time to see if they show a trend which would indicate an unstable measurement. In the sample you propose you are more likely to see differences based on compensation variation between your 3 populations which again is independent of the number of events looked at if more than 500 have been measured. It would indeed be interesting to see how much variation you get in mfi between independent tubes as you are more likely to increase variation by the sample processing than by the measurement. One control for your experiment would be to test the MFI distribution by swapping fluorochromes to demonstrate that you are independent of compensation and to show that the change in MFI is not dependent of steric hindrance or FRET, depending of the experimental / instrument details. Regards Gerhard > -----Original Message----- > From: Stevan Lauriault [mailto:ste...@lauriault.com] > Sent: 07 February 2007 15:16 > To: Cytometry Mailing List > Subject: Re: gate-specific data cropping > Hi and thanks, > Since we want to directly compare MFIs (in FL2) between any > two subsets (R1 vs. R3) that are isolated on a bivariate dot [quoted text clipped - 3 lines] > Directly comparing the subsets from the same sample tube, and > even better, the same data set, would eliminate this extra unknown. > Let us say we have acquired 100,000 total events (stained > with 3 fluorochromes, measured in FL1, FL2, and FL4 [quoted text clipped - 4 lines] > Within those 100,000 total events, there are 500 of R1, 700 > of R2 and 1200 of R3. > If we crop the 100,000 total events to 75,000 total events > (from the top in stack-collected data), there will become > exactly 500 events in the R2 region. If we crop the 100,000 > total events to 40,000 total events, there will become 500 > events in R3. > Now the three populations; R1, R2, and R3 each have exactly > 500 total events, and we can directly compare MFIs among the > subsets that have identical sample sizes. We could also then > say that, when comparing means between these subsets under > identical experimental conditions, Rx gives a consistently > higher fluorescent signal than Ry. > Is this a reasonable strategy? Is there any software > available that can perform this > function? > Kind Regards, > Stevan Lauriault > ---------- Original Message ---------------------------------- > From: "Byron Ellis" <bcel...@stanford.edu> > Date: Tue, 6 Feb 2007 12:17:35 -0800 > >Speaking as a statistician, I would not count running the > same tube 3 [quoted text clipped - 8 lines] > >to experimental error as well as the biological variability of the > >cells and a single sample preparation only captures the latter. > >On 2/5/07, Stevan Lauriault <ste...@lauriault.com> wrote: > >> Dear All, > >> Question: > >> We are isolating leukocyte subsets to measure and compare relative > >> levels of [quoted text clipped - 7 lines] > intensities of a > >> biomarker, among the subsets. > >> To get statistically comparable sample sizes among these > subsets, we [quoted text clipped - 17 lines] > R2, > >> and R3). > >> Scientifically, this shouldn't be a problem, since flow cytometry > >> data is collected randomly within the same sample tube. Not only [quoted text clipped - 5 lines] > >> can see why some scientists might be uneasy with the idea of > >> manipulating raw list-mode data. > >> However, Since the data is collected randomly from the > same sample, [quoted text clipped - 3 lines] > >> be cropping (from stack-collected data) the most recent events > >> collected. > >> Currently, we are acquiring 3 different data files for > each tube, and [quoted text clipped - 3 lines] > function with > >> any current flow cytometry data analysis software? > >> Kind Regards, > >> Stevan Lauriault > >> ________________________________________________________________ > >> Sent via the WebMail system at lauriault.com > >-- > >Byron Ellis (byron.el...@gmail.com) > >"Oook" -- The Librarian > ________________________________________________________________ > Sent via the WebMail system at lauriault.com Received on Thu Feb 8 14:38:00 2007 * This message: [ Message body ] * Next message: Byron Ellis: "Re: gate-specific data cropping" * Previous message: Joanne Lannigan: "Live/Dead Stain Kit Correction" * Maybe in reply to: Stevan Lauriault: "gate-specific data cropping" * Next in thread: Stevan Lauriault: "Re: gate-specific data cropping" * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST Re: gate-specific data cropping * This message: [ Message body ] [ More options ] * Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] From: Byron Ellis <bcel...@stanford.edu> Date: Wed Feb 07 2007 - 19:21:28 EST On 2/7/07, Stevan Lauriault <ste...@lauriault.com> wrote: > Hi and thanks, > Since we want to directly compare MFIs (in FL2) between any two subsets (R1 vs. R3) that are isolated on a bivariate dot plot (FL1 vs. FL4), doing so in separate sample tubes would introduce the extra probability of experimental error between sample tubes. Directly comparing the subsets from the same sample tube, and even better, the same data set, would eliminate this extra unknown. It doesn't necessarily eliminate the unknown, it may just keep you from finding out about it. If you were to prepare one sample on Monday, see significant differences, celebrate, etc and then on Wednesday prepare another sample where you didn't you'd probably want to know that (and figure out why). Simply taking the FACS tube off the cytometer and putting it back on won't give you that information. Now, would I be surprised if that actually happened in your case? Yes. Would I do replicates anyway? Yes. > Let us say we have acquired 100,000 total events (stained with 3 fluorochromes, measured in FL1, FL2, and FL4 respectively). We are using a bivariate dot plot of FL1 vs. FL4 in which we are isolating 3 subsets (R1, R2, and R3) based on their relative expressions of FL1 vs. FL4. We then want to compare the MFIs, as measured in FL2, among these subsets. Within those 100,000 total events, there are 500 of R1, 700 of R2 and 1200 of R3. > If we crop the 100,000 total events to 75,000 total events (from the top in stack-collected data), there will become exactly 500 events in the R2 region. If we crop the 100,000 total events to 40,000 total events, there will become 500 events in R3. > Now the three populations; R1, R2, and R3 each have exactly 500 total events, and we can directly compare MFIs among the subsets that have identical sample sizes. We could also then say that, when comparing means between these subsets under identical experimental conditions, Rx gives a consistently higher fluorescent signal than Ry. Well, depending on what you're doing (you've never said how you intend to compare means), equal sample sizes is not particularly required so there's no particular reason to cut the data to obtain equal sample sizes. For example, one-way ANOVA (or one-way ANOVA with repeated measure in the event that you chose to do _real_ replicates), which simply says "they're different" (there are other tests, variants of one-sided t-tests essentially, that give you directionality statements such as mu_{R_1} > mu_{R_2} ). In this case your assumptions are: 1. Each group is independent 2. Normality of group populations (or at least "close enough" to Normal) 3. The _population_ variances of the dependent variable for each group are equal (or pretty close). (Actually check this. It would be unsurprising to encounter large differences in the population variances among your different groups). Like I say, it depends a bit on what you intend to do for your analysis. For example, _two_-way ANOVA actually expects equal sample sizes. > Is this a reasonable strategy? Is there any software available that can perform this function? On an FCS file directly? Probably not (neither the manipulation of the flow data nor the testing). Once you've got the data out of FCS form and appropriately labeled with group membership or split into separate files or something similar, then any reasonable statistics package (Excel is not included in the list of reasonable statistics packages) can perform the statistical tests. Personally, I use R (http://www.r-project.org) for analyzing all the flow data I have, but it can be intimidating for new users (though extremely powerful once you learn to use it). There are two packages in R for manipulating flow data at the moment (prada and rflowcyt), available through the Bioconductor Project (http://www.bioconductor.org) that can actually read in and manipulate FCS data directly or slightly manipulated data from say FlowJo (so you can do your gating there for example). I'm also part of a group made up of the people who did rflowcyt and prada that are making a combined package of the best bits of both (and some other new bits) that we expect to be available in the next Bioconductor release (probably sometime in April). Hope that helps, Byron > Kind Regards, > Stevan Lauriault > ---------- Original Message ---------------------------------- > From: "Byron Ellis" <bcel...@stanford.edu> > Date: Tue, 6 Feb 2007 12:17:35 -0800 > >Speaking as a statistician, I would not count running the same tube 3 > >times or simply cutting the FCS file (the two are equivalent) as three [quoted text clipped - 4 lines] > >due to experimental error as well as the biological variability of the > >cells and a single sample preparation only captures the latter. > >On 2/5/07, Stevan Lauriault <ste...@lauriault.com> wrote: > >> Dear All, > >> Question: > >> We are isolating leukocyte subsets to measure and compare relative levels of expression > >> of certain antigens. For example, there are three subsets within a bivariate dot plot; > >> gated on R1, R2 and R3 respectively, and we would like to statistically analyze the > >> relative mean fluorescence intensities of a biomarker, among the subsets. > >> To get statistically comparable sample sizes among these subsets, we are running the same > >> tube three times and changing the storage criteria to 500 events for each subset > >> (example, the first run is 500 events of R1, the second 500 of R2, and the third is 500 > >> of R3). Instead of repeating the same tube three times, and wasting valuable sample, it > >> seems like a good idea to "crop" a preexisting data file of, for example, 100,000 total > >> events, three times to get an identical sample size for all three gated subsets (R1, R2, > >> and R3). > >> Scientifically, this shouldn't be a problem, since flow cytometry data is collected > >> randomly within the same sample tube. Not only could we run one acquisition to get > >> different monocyte subset populations, but also to statistically compare constant numbers > >> of monocytes, PMNs, lymphocytes, lymphocyte subsets, etc. However, I can see why some > >> scientists might be uneasy with the idea of manipulating raw list-mode data. > >> However, Since the data is collected randomly from the same sample, doing this should > >> give exactly the same readings as if we just collected less events, since we would only > >> be cropping (from stack-collected data) the most recent events collected. > >> Currently, we are acquiring 3 different data files for each tube, and adjusting the > >> acquisition and storage settings for each acquisition. Is there a way to perform a > >> gate-specific data cropping function with any current flow cytometry data analysis > >> software? > >> Kind Regards, > >> Stevan Lauriault > >> ________________________________________________________________ > >> Sent via the WebMail system at lauriault.com > >-- > >Byron Ellis (byron.el...@gmail.com) > >"Oook" -- The Librarian > ________________________________________________________________ > Sent via the WebMail system at lauriault.com -- Byron Ellis (byron.el...@gmail.com) "Oook" -- The Librarian Received on Thu Feb 8 14:58:00 2007 * This message: [ Message body ] * Next message: Kathy Heel: "Sorting RGCs" * Previous message: Nebe-Von-Caron, G: "RE: gate-specific data cropping" * In reply to: Stevan Lauriault: "Re: gate-specific data cropping" * Next in thread: Nebe-Von-Caron, G: "RE: gate-specific data cropping" * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST Re: gate-specific data cropping * This message: [ Message body ] [ More options ] * Related messages: [ Next message ] [ Previous message ] [ Maybe in reply to ] [ Next in thread ] [ Replies ] From: Stevan Lauriault <ste...@lauriault.com> Date: Wed Feb 07 2007 - 10:16:03 EST Hi and thanks, Since we want to directly compare MFIs (in FL2) between any two subsets (R1 vs. R3) that are isolated on a bivariate dot plot (FL1 vs. FL4), doing so in separate sample tubes would introduce the extra probability of experimental error between sample tubes. Directly comparing the subsets from the same sample tube, and even better, the same data set, would eliminate this extra unknown. Let us say we have acquired 100,000 total events (stained with 3 fluorochromes, measured in FL1, FL2, and FL4 respectively). We are using a bivariate dot plot of FL1 vs. FL4 in which we are isolating 3 subsets (R1, R2, and R3) based on their relative expressions of FL1 vs. FL4. We then want to compare the MFIs, as measured in FL2, among these subsets. Within those 100,000 total events, there are 500 of R1, 700 of R2 and 1200 of R3. If we crop the 100,000 total events to 75,000 total events (from the top in stack-collected data), there will become exactly 500 events in the R2 region. If we crop the 100,000 total events to 40,000 total events, there will become 500 events in R3. Now the three populations; R1, R2, and R3 each have exactly 500 total events, and we can directly compare MFIs among the subsets that have identical sample sizes. We could also then say that, when comparing means between these subsets under identical experimental conditions, Rx gives a consistently higher fluorescent signal than Ry. Is this a reasonable strategy? Is there any software available that can perform this function? Kind Regards, Stevan Lauriault ---------- Original Message ---------------------------------- From: "Byron Ellis" <bcel...@stanford.edu> Date: Tue, 6 Feb 2007 12:17:35 -0800 >Speaking as a statistician, I would not count running the same tube 3 >times or simply cutting the FCS file (the two are equivalent) as three [quoted text clipped - 4 lines] >due to experimental error as well as the biological variability of the >cells and a single sample preparation only captures the latter. >On 2/5/07, Stevan Lauriault <ste...@lauriault.com> wrote: >> Dear All, >> Question: >> We are isolating leukocyte subsets to measure and compare relative levels of expression >> of certain antigens. For example, there are three subsets within a bivariate dot plot; >> gated on R1, R2 and R3 respectively, and we would like to statistically analyze the >> relative mean fluorescence intensities of a biomarker, among the subsets. >> To get statistically comparable sample sizes among these subsets, we are running the same >> tube three times and changing the storage criteria to 500 events for each subset >> (example, the first run is 500 events of R1, the second 500 of R2, and the third is 500 >> of R3). Instead of repeating the same tube three times, and wasting valuable sample, it >> seems like a good idea to "crop" a preexisting data file of, for example, 100,000 total >> events, three times to get an identical sample size for all three gated subsets (R1, R2, >> and R3). >> Scientifically, this shouldn't be a problem, since flow cytometry data is collected >> randomly within the same sample tube. Not only could we run one acquisition to get >> different monocyte subset populations, but also to statistically compare constant numbers >> of monocytes, PMNs, lymphocytes, lymphocyte subsets, etc. However, I can see why some >> scientists might be uneasy with the idea of manipulating raw list-mode data. >> However, Since the data is collected randomly from the same sample, doing this should >> give exactly the same readings as if we just collected less events, since we would only >> be cropping (from stack-collected data) the most recent events collected. >> Currently, we are acquiring 3 different data files for each tube, and adjusting the >> acquisition and storage settings for each acquisition. Is there a way to perform a >> gate-specific data cropping function with any current flow cytometry data analysis >> software? >> Kind Regards, >> Stevan Lauriault >> ________________________________________________________________ >> Sent via the WebMail system at lauriault.com >-- >Byron Ellis (byron.el...@gmail.com) >"Oook" -- The Librarian ________________________________________________________________ Sent via the WebMail system at lauriault.com Received on Wed Feb 7 20:38:00 2007 * This message: [ Message body ] * Next message: Wilson Mandala: "Re: detection of Tregs in PHA/ PMA stimulated cultures of CD T cells" * Previous message: Matthew Hanson: "Unique opportunity for the right person at the UW-Madison!" * Maybe in reply to: Stevan Lauriault: "gate-specific data cropping" * Next in thread: Byron Ellis: "Re: gate-specific data cropping" * Reply: Byron Ellis: "Re: gate-specific data cropping" * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST Re: gate-specific data cropping * This message: [ Message body ] [ More options ] * Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] From: Byron Ellis <bcel...@stanford.edu> Date: Tue Feb 06 2007 - 15:17:35 EST Speaking as a statistician, I would not count running the same tube 3 times or simply cutting the FCS file (the two are equivalent) as three replicates so I wouldn't do either to achieve what you want (though I can imagine situations where you would resample to calculate statistics). To get three replicates you would have to prepare three separate samples---you're interested in the variability of the mean due to experimental error as well as the biological variability of the cells and a single sample preparation only captures the latter. On 2/5/07, Stevan Lauriault <ste...@lauriault.com> wrote: > Dear All, > Question: > We are isolating leukocyte subsets to measure and compare relative levels of expression > of certain antigens. For example, there are three subsets within a bivariate dot plot; > gated on R1, R2 and R3 respectively, and we would like to statistically analyze the > relative mean fluorescence intensities of a biomarker, among the subsets. > To get statistically comparable sample sizes among these subsets, we are running the same > tube three times and changing the storage criteria to 500 events for each subset > (example, the first run is 500 events of R1, the second 500 of R2, and the third is 500 > of R3). Instead of repeating the same tube three times, and wasting valuable sample, it > seems like a good idea to "crop" a preexisting data file of, for example, 100,000 total > events, three times to get an identical sample size for all three gated subsets (R1, R2, > and R3). > Scientifically, this shouldn't be a problem, since flow cytometry data is collected > randomly within the same sample tube. Not only could we run one acquisition to get > different monocyte subset populations, but also to statistically compare constant numbers > of monocytes, PMNs, lymphocytes, lymphocyte subsets, etc. However, I can see why some > scientists might be uneasy with the idea of manipulating raw list-mode data. > However, Since the data is collected randomly from the same sample, doing this should > give exactly the same readings as if we just collected less events, since we would only > be cropping (from stack-collected data) the most recent events collected. > Currently, we are acquiring 3 different data files for each tube, and adjusting the > acquisition and storage settings for each acquisition. Is there a way to perform a > gate-specific data cropping function with any current flow cytometry data analysis > software? > Kind Regards, > Stevan Lauriault > ________________________________________________________________ > Sent via the WebMail system at lauriault.com -- Byron Ellis (byron.el...@gmail.com) "Oook" -- The Librarian Received on Wed Feb 7 18:58:00 2007 * This message: [ Message body ] * Next message: RAJA ALAMELU: "Storage of fixed cells before acquisition" * Previous message: Eric Van Buren: "Re: gate-specific data cropping" * In reply to: Stevan Lauriault: "gate-specific data cropping" * Next in thread: Stevan Lauriault: "Re: gate-specific data cropping" * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST Re: gate-specific data cropping * This message: [ Message body ] [ More options ] * Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] From: Eric Van Buren <eric.vanbu...@wayne.edu> Date: Wed Feb 07 2007 - 10:57:01 EST Stevan, It's not exactly what you're looking for, but the easiest "off the shelf" solution may be to acquire time as a parameter. Or, if your software allows, use the simulated time parameter. Gate on time empirically to produce the the desired number of events in each region. As you say, the sampling is random, so it shouldn't matter which "time slice" you choose. However, to keep a uniform protocol you may want to always start your time gate with time equals zero, i.e. starting at the beginning of the list mode file. This should minimize any time-related artifacts, such as sample settling, sample/sheath dye equilibrium, exposure to room light, temperature, etc. --Eric >Dear All, >Question: >We are isolating leukocyte subsets to measure and compare relative levels of expression >of certain antigens. For example, there are three subsets within a bivariate dot plot; >gated on R1, R2 and R3 respectively, and we would like to statistically analyze the >relative mean fluorescence intensities of a biomarker, among the subsets. >To get statistically comparable sample sizes among these subsets, we are running the same >tube three times and changing the storage criteria to 500 events for each subset >(example, the first run is 500 events of R1, the second 500 of R2, and the third is 500 >of R3). Instead of repeating the same tube three times, and wasting valuable sample, it >seems like a good idea to "crop" a preexisting data file of, for example, 100,000 total >events, three times to get an identical sample size for all three gated subsets (R1, R2, >and R3). >Scientifically, this shouldn't be a problem, since flow cytometry data is collected >randomly within the same sample tube. Not only could we run one acquisition to get >different monocyte subset populations, but also to statistically compare constant numbers >of monocytes, PMNs, lymphocytes, lymphocyte subsets, etc. However, I can see why some >scientists might be uneasy with the idea of manipulating raw list-mode data. >However, Since the data is collected randomly from the same sample, doing this should >give exactly the same readings as if we just collected less events, since we would only >be cropping (from stack-collected data) the most recent events collected. >Currently, we are acquiring 3 different data files for each tube, and adjusting the >acquisition and storage settings for each acquisition. Is there a way to perform a >gate-specific data cropping function with any current flow cytometry data analysis >software? >Kind Regards, >Stevan Lauriault >________________________________________________________________ >Sent via the WebMail system at lauriault.com Eric Van Buren <eric.vanbu...@wayne.edu> Manager, Flow Cytometry Core Facility Karmanos Cancer Institute Detroit, Michigan, USA Received on Wed Feb 7 18:38:01 2007 * This message: [ Message body ] * Next message: Byron Ellis: "Re: gate-specific data cropping" * Previous message: RAJA ALAMELU: "FACSort Macintosh conked off!" * In reply to: Stevan Lauriault: "gate-specific data cropping" * Next in thread: Byron Ellis: "Re: gate-specific data cropping" * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST gate-specific data cropping * This message: [ Message body ] [ More options ] * Related messages: [ Next message ] [ Previous message ] [ Next in thread ] [ Replies ] From: Stevan Lauriault <ste...@lauriault.com> Date: Mon Feb 05 2007 - 13:56:18 EST Dear All, Question: We are isolating leukocyte subsets to measure and compare relative levels of expression of certain antigens. For example, there are three subsets within a bivariate dot plot; gated on R1, R2 and R3 respectively, and we would like to statistically analyze the relative mean fluorescence intensities of a biomarker, among the subsets. To get statistically comparable sample sizes among these subsets, we are running the same tube three times and changing the storage criteria to 500 events for each subset (example, the first run is 500 events of R1, the second 500 of R2, and the third is 500 of R3). Instead of repeating the same tube three times, and wasting valuable sample, it seems like a good idea to "crop" a preexisting data file of, for example, 100,000 total events, three times to get an identical sample size for all three gated subsets (R1, R2, and R3). Scientifically, this shouldn't be a problem, since flow cytometry data is collected randomly within the same sample tube. Not only could we run one acquisition to get different monocyte subset populations, but also to statistically compare constant numbers of monocytes, PMNs, lymphocytes, lymphocyte subsets, etc. However, I can see why some scientists might be uneasy with the idea of manipulating raw list-mode data. However, Since the data is collected randomly from the same sample, doing this should give exactly the same readings as if we just collected less events, since we would only be cropping (from stack-collected data) the most recent events collected. Currently, we are acquiring 3 different data files for each tube, and adjusting the acquisition and storage settings for each acquisition. Is there a way to perform a gate-specific data cropping function with any current flow cytometry data analysis software? Kind Regards, Stevan Lauriault ________________________________________________________________ Sent via the WebMail system at lauriault.com Received on Tue Feb 6 13:18:00 2007 * This message: [ Message body ] * Next message: Beverly Barton: "Re: Equipment Survey" * Previous message: Lora Barsky: "Re: Cell Quest pro and non CellQuest data" * Next in thread: Eric Van Buren: "Re: gate-specific data cropping" * Reply: Eric Van Buren: "Re: gate-specific data cropping" * Reply: Byron Ellis: "Re: gate-specific data cropping" * Maybe reply: Stevan Lauriault: "Re: gate-specific data cropping" * Maybe reply: Nebe-Von-Caron, G: "RE: gate-specific data cropping" * Maybe reply: Stevan Lauriault: "Re: gate-specific data cropping" * Maybe reply: VSH Tech Support: "RE: gate-specific data cropping" * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST RE: DNA analysis software * This message: [ Message body ] [ More options ] * Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] From: Novo, David <david.n...@denovosoftware.com> Date: Fri Feb 16 2007 - 19:05:22 EST Hello Ibtissam, Multicycle is a very commonly used DNA analysis program. It can automatically detect the number of aneuploid populations that you have (if any) and runs them through several different models with different fitting parameters to help you determine which is the best one for your data. It also has a wide range of debris and aggregate compensation to allow it to be used with a wide variety of sample preparation methods. Multicycle has just been incorporated as a plug in module to FCS Express, so that you get the benefits of the high end DNA analysis as well as the ease of use and gating/presentation abilities of FCS Express. It really makes DNA analysis a snap. There is some information available at http://www.denovosoftware.com/site/Multicycleplugin.shtml -Dave ----------------------------------- David Novo President De Novo Software david.n...@denovosoftware.com ________________________________ From: Ibtissam Abdul-Jabbar [mailto:iajab...@cicr.uq.edu.au] Sent: Wednesday, February 14, 2007 8:41 PM To: cyto-inbox Subject: DNA analysis software Dear All, before buying software to analyse DNA on PC, I would like to get your opinion. I already have ModFit for Macintosh. What are you using and what do you recommend for research purposes. Is MultiCycle Av the one of choice? I appreciate your comments. Ibtissam A Jabbar (PhD) Manager of the FACS facilities Diamantina Institute for Cancer, Immunology and Metabolic Medicine (DI) The University of Queensland Level 4 R Wing Princess Alexandra Hospital Ipswich Rd Buranda QLD 4102 Australia Ph: 07 3240 5945 Fax: 07 3240 5946 Mob: 0401154744 Received on Mon Feb 19 13:38:00 2007 * This message: [ Message body ] * Next message: mmo...@kent.edu: "presenting flow data" * Previous message: Derek Davies: "Re: tecnicad el ciclo celular" * In reply to: Ibtissam Abdul-Jabbar: "DNA analysis software" * Next in thread: VSH Tech Support: "RE: DNA analysis software" * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST DNA analysis software * This message: [ Message body ] [ More options ] * Related messages: [ Next message ] [ Previous message ] [ Next in thread ] [ Replies ] From: Ibtissam Abdul-Jabbar <iajab...@cicr.uq.edu.au> Date: Wed Feb 14 2007 - 23:41:15 EST Dear All, before buying software to analyse DNA on PC, I would like to get your opinion. I already have ModFit for Macintosh. What are you using and what do you recommend for research purposes. Is MultiCycle Av the one of choice? I appreciate your comments. Ibtissam A Jabbar (PhD) Manager of the FACS facilities Diamantina Institute for Cancer, Immunology and Metabolic Medicine (DI) The University of Queensland Level 4 R Wing Princess Alexandra Hospital Ipswich Rd Buranda QLD 4102 Australia Ph: 07 3240 5945 Fax: 07 3240 5946 Mob: 0401154744 Received on Thu Feb 15 12:38:00 2007 * This message: [ Message body ] * Next message: moo...@mail.MED.UPENN.EDU: "Fwd: Re: early apoptotic cells" * Previous message: FACSLAB: "Northern Flow Group (UK)" * Next in thread: Novo, David: "RE: DNA analysis software" * Reply: Novo, David: "RE: DNA analysis software" * Reply: VSH Tech Support: "RE: DNA analysis software" * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST FACSCalibur - developing software for Macintosh * This message: [ Message body ] [ More options ] * Related messages: [ Next message ] [ Previous message ] From: Simon Crase <Simon.Cr...@invetech.com.au> Date: Tue Feb 13 2007 - 16:32:34 EST I've developed software to analyze data from a FACSCalibur, and our client has asked whether we can port it to run on the FACSCalibur itself. I understand the FACSCalibur includes a Macintosh. I'm interested in knowing whether anyone has done this before, especially with Java, and what pitfalls they encountered. Simon A. Crase Invetech Pty Ltd Private Bag 44 495 Blackburn Road Mount Waverley Vic 3149 Phone: 61 3 9211 7933 Mobile: 0424 782 725 Fax: 61 3 9211 7702 (facsimile) e-mail: simon.cr...@invetech.com.au ___________________________________________________________ IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this e.mail are those of the individual sender, and not necessarily those of Vision Systems Limited]. Received on Wed Feb 14 16:18:00 2007 * This message: [ Message body ] * Next message: William King: "Re: early apoptotic cells" * Previous message: gharago...@sums.ac.ir: "correlation between cell cycle and cell proliferation, a question" * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST Flow analysis software * This message: [ Message body ] [ More options ] * Related messages: [ Next message ] [ Previous message ] From: Xiangle Sun <sunxian...@yahoo.com> Date: Wed Sep 12 2007 - 17:12:44 EDT Hi, Flowers, We just got BD LSRII flow cytometer with FACSDiva software. I know Flowjo is a popular software and some people use FlowJo instead of FACSDiva to do data analysis even it comes with instrument. My questions are: 1. What are the advantages of FlowJo and FACSDiva. Then is it worth of purchasing FlowJo to do analysis? 2. How about the feature of FlowJo and FACSDiva on DNA cycle analysis? 3. Are there any other better analysis softwares? either for multicolor phenotyping, DNA cycle or both? Any comments that based on your experience will be greatly appreciated! Xiangle Sun ____________________________________________________________________________________ Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. http://mobile.yahoo.com/go?refer=1GNXIC Received on Thu Sep 13 11:18:00 2007 * This message: [ Message body ] * Next message: Jose Benito: "malaria" * Previous message: Ray Hester: "entire membrane fluor vs capped - equally bright by FACS?" * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST RE: CFSE proliferation software * This message: [ Message body ] [ More options ] * Related messages: [ Next message ] [ Previous message ] [ Maybe in reply to ] From: user facs_copy <facs_c...@wehi.EDU.AU> Date: Wed Sep 19 2007 - 02:03:28 EDT Michael, Depends what you mean by "analyze". If you mean just to extract cell numbers at each division state, we use our own Weasel program for that (see http://www.wehi.edu.au/cytometry/WEASELv2.html , scroll to the bottom of the page). We also have a proliferation modelling program, CytonCalculator, that can take that proliferation data and fit a model calculation to it (see http://www.wehi.edu.au/WEHI_Groups/indexworkgroups.php?id=115 for the entry point that describes the process). CytonCalculator may be downloaded free. You'll find the download link on that page. Frank Battye. > From: Kalos, Michael > Sent: Thursday, September 13, 2007 5:39 PM > No doubt this has been brought up before: I am looking for software > recommendations to analyze data for measuring proliferation using CFSE. > Our data is acquired on an FC500 and our computers are PC-based. | | < The Cytometry Laboratory \__/ <<<<< The Walter & Eliza Hall Institute ------!!<<<<<<<< Post Office, Royal Melbourne Hospital /!!\ <<<<< Victoria 3050, Australia o !! \ < ph: 61_3_9345 2541, fax: 61_3_9347 0852 Received on Wed Sep 19 11:38:00 2007 * This message: [ Message body ] * Next message: pearly.yong....@nhc.com.sg: "Histogram overlay and exporting fcs 2.0 files question" * Previous message: Maria Laura: "RE: single cell sorting" * Maybe in reply to: Kalos, Michael: "RE: CFSE proliferation software" * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST RE: CFSE proliferation software * This message: [ Message body ] [ More options ] * Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] From: <moo...@mail.MED.UPENN.EDU> Date: Mon Sep 17 2007 - 19:20:03 EDT I also agree. We do a lot of proliferation studies and have found that Proliferation Wizard on Modfit is very good. Jonni Quoting James F George <jgeo...@uab.edu>: > I have found the Modfit software from Verity to be quite useful for > this, and it integrates with Winlist rather nicely, if you have it. Don > at Verity has been very helpful over the years in helping us implement > the software for routine analyses. > I highly recommend this company. > I have no financial interest in Verity. I am just a long-time user. > James F. George, PhD | Professor > Division of Cardiothoracic Surgery | University of Alabama at > Birmingham > 701 South 19th St. | Birmingham, AL 35294 > Phone: (205) 934-4261 | Fax: (205) 975-0085 > Email: jgeo...@uab.edu <mailto:jgeo...@uab.edu> > UAB HEALTH SYSTEM > Medicine that touches the world On Jan 31, 6:39 pm, buybro...@yahoo.com wrote: > > RE: DNA analysis software > [quoted text clipped - 258 lines] > > - Show quoted text - DNA SOFTWARE MESSAGES WERE TO BE DELETED THE SMALL COMPANY MAY JUST BE KANECKI ASSOCIATES INC. THAT DISCOVERED THE PURDUE CYTOMETRY MAIL LIST AFTER BEING CALLED SCAMMERS BY J PAUL ROBINSON FOR INTRODUCING NEW SOFTWARE SENT TO ROBERT MURPHY! DID ROBERT MURPHY GET THE EMAIL OR DID J PAUL ROBINSON INTERCEPT HIS EMAIL? > PURDUE CYTOMETRY AND J PAUL ROBINSON HAS CONTROLED THE MARKET OF > SOFTWARE! > HOW PURDUE TAKES ADVANTAGE OF THE CYTOMETRY MAIL LIST AND YES THE TALK > ABOUT HOW TO FILTER MAIL...KEEP THE SMALL COMPANIES OUT! > READ ABOUT HOW PURDUE KEEPS ALL SOFTWARE SALES AND VENDORS WITHIN YES > THEY TALK ABOUT FILTERING THE MAIL LIST AND VENDORS! The Purdue Cytometry Mail List Evidence is being DELETED.. DO NOT LET THEM DESTROY THE EVIDENCE From: J. Paul Robinson <j...@flowcyt.cyto.purdue.edu> Date: Fri Dec 28 2007 - 13:43:46 EST Beware, the end is nigh! No, not an apocalyptic prediction - but 2007 is definitely coming to an end. Not before time I would say - it s been a busy year. But I have some strong words to end the year and I am going to say them!! Of course you don t have to read them! Cytometry is now 40 years old and it s been sort of decaying a bit. Wake up people - times are changing - look at all these new small companies trying to stick their noses in "our" field! Wake up people - times are changing - look at all these new small companies trying to stick their noses in "our" field! Wake up people - times are changing - look at all these new small companies trying to stick their noses in "our" field! Wake up people - times are changing - look at all these new small companies trying to stick their noses in "our" field! Wake up people - times are changing - look at all these new small companies trying to stick their noses in "our" field! Wake up people - times are changing - look at all these new small companies trying to stick their noses in "our" field! *************************************************************************** ********************************************** THE SMALL COMPANY MAY JUST BE KANECKI ASSOCIATES INC. THAT DISCOVERED THE PURDUE CYTOMETRY MAIL LIST AFTER BEING CALLED SCAMMERS BY J PAUL ROBINSON FOR INTRODUCING NEW SOFTWARE SENT TO ROBERT MURPHY! DID ROBERT MURPHY GET THE EMAIL OR DID J PAUL ROBINSON INTERCEPT HIS EMAIL? WHY ARE THE LINKS DISCOVERED BEING REMOVED AND ARTICLES TOO? GOOGLE EXPOSES THE TRUTH BY POSTING THE UNFILTERED RECORDS...READ ARTICLES HOW PURDUE DISCUSSES HOW TO **FILTER** THE MAIL LIST AND KEEP VENDORS OUT! *************************************************************************** ************************************************** ... Purdue Cytometry Mailing List: Re: 2008 NW Regional Cytometry Meeting, March 13 - 15 in Portland ... Technology, IntelliCyt, Miltenyi Biotec, Partec, StemCell Technologies, Tree Star, Union Biometrica, and Verity Software House. ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1946.htm - 6.9KB 74% 09 Dec 07 Find Similar Highlight Purdue Cytometry Mailing List: New England Cytometry Users Group Meeting - Thanks! ... Purdue Cytometry Mailing List: New England Cytometry Users Group Meeting - Thanks! ... C. Bruce Bagwell, MD, Ph.D. - Verity Software House ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1593.htm - 7.1KB 74% 25 Oct 07 Find Similar Highlight Purdue Cytometry Mailing List: New England Cytometry Users Group annual meeting reminder ... Purdue Cytometry Mailing List: New England Cytometry Users Group ... Bruce Bagwell, MD, Ph.D. - Verity Software House "Probability State Models: A ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1546.htm - 7.4KB 74% 17 Oct 07 Find Similar Highlight Purdue Cytometry Mailing List: New England Cytometry Users Group annual meeting reminder ... Purdue Cytometry Mailing List: New England Cytometry Users Group ... Bruce Bagwell, MD, Ph.D. - Verity Software House "Probability State Models: A ... http://www.cyto.purdue.edu/hmarchiv/current/1546.htm - 7.4KB 74% 17 Oct 07 Find Similar Highlight Document count: purdue (139318) cytometry (25468) mail (53709) list (63448) 2007 (37553) verity (222) software (15216) sales (14402) 07 (21574) by (129519) thread (26375) purdue cytometry mail list 2007 verity software sales 07 by... (27962) about 187244 results found, top 500 sorted by relevance score using date hide summaries group by location spacer 1-10 next Purdue Cytometry Mailing List: RE: DNA analysis software ... Purdue Cytometry Mailing List: RE: DNA analysis software ... J. Herbert Technical Support Manager Verity Software House ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0747.htm - 7.7KB 78% 26 May 07 Find Similar Highlight Purdue Cytometry Mailing List: Re: DNA analysis software ... vsh.com] > Sent: Wednesday, February 21, 2007 10:30 AM > To: Cytometry Mailing List > Subject: RE: DNA ... J. Herbert > Technical Support Manager > Verity Software House ... http://www.cyto.purdue.edu/hmarchiv/Current/0755.htm - 8.6KB 78% 26 May 07 Find Similar Highlight Purdue Cytometry Mailing List: Announcing User Forum at Verity Software House, Inc. ... Purdue Cytometry Mailing List: Announcing User Forum at Verity ... Verity Software House is pleased to announce the creation of a Verity User's Forum on our ... http://www.cyto.purdue.edu/hmarchiv/2002/0542.htm - 4.6KB 78% 12 Mar 02 Find Similar Highlight Purdue Cytometry Mailing List: RE: FL2H in PI cell cycle analysis ... From: Verity Software House <ver...@vsh.com> Date: Fri Sep 07 2007 - 17:30:59 EDT ... From: Verity Software House <ver...@vsh.com> Date: Fri Sep 07 2007 - 17:30:59 EDT ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1351.htm - 7.2KB 77% 12 Sep 07 Find Similar Highlight Purdue Cytometry Mailing List: By Date ... Purdue Cytometry Mailing List By Thread ... Jim (NIH/NIAMS) (Wed Dec 19 2007 - 13:41:51 EST) ... 13th Leipziger Workshop, 2nd Call and Seasonal Greetings Dr. Attila Tarnok (Wed Dec 19 2007 - 07:41:10 EST) ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/thread.htm - 328.1KB 76% 21 Dec 07 Find Similar Highlight Purdue Cytometry Mailing List: DNA analysis software ... Purdue Cytometry Mailing List: DNA analysis software ... we offer a conversion at no charge. I invite you to contact Verity Software House directly with any specific questions or issues. Best regards, Don Donald ... http://www.cyto.purdue.edu/hmarchiv/Current/0753.htm - 5.6KB 76% 26 May 07 Find Similar Highlight Purdue Cytometry Mailing List: By Date ... Purdue Cytometry Mailing List: By Date ... Wed Dec 19 2007 - 07:41:10 EST ... http://www.cyto.purdue.edu/hmarchiv/Current/ - 362.4KB 75% 21 Dec 07 Find Similar Highlight Purdue Cytometry Mailing List: By Date ... Purdue Cytometry Mailing List: By Date ... Mon Sep 24 2007 - 22:25:06 EDT) ... Re: mCherry on a Calibur ??!! (Thu Aug 30 2007 - 19:36:32 EDT) ... Re: DiI and FITC on a Vantage (Tue Aug 07 2007 - 20:07:02 EDT) ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/author.htm - 300.2KB 75% 21 Dec 07 Find Similar Highlight Purdue Cytometry Mailing List: By Date ... Purdue Cytometry Mailing List: By Date ... Derek Davies (Wed Mar 07 2007 - 07:56:30 EST) ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/subject.htm - 289.4KB 75% 21 Dec 07 Find Similar Highlight Purdue Cytometry Mailing List: Cytometry software for linux ... Purdue Cytometry Mailing List: Cytometry software for linux ... Previous message: Verity Software House: "RE: FL2H in PI cell cycle analysis" ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1352.htm - 5.5KB 75% 13 Sep 07 Find Similar about 187243 results found, top 500 sorted by relevance score using date hide summaries group by location prev 21-30 next Purdue Cytometry Mailing List: Re: FL2H in PI cell cycle analysis ... Purdue Cytometry Mailing List: Re: FL2H in PI cell cycle analysis ... Next in thread: Verity Software House: "RE: FL2H in PI cell cycle analysis" ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1336.htm - 6.6KB 74% 11 Sep 07 Find Similar Highlight Purdue Cytometry Mailing List: New England Cytometry Users Group annual meeting preliminary ... ... Purdue Cytometry Mailing List: New England Cytometry Users Group annual meeting preliminary announcement ... up of speakers this year: C. Bruce Bagwell, MD, Ph.D. - Verity Software House http://www.vsh.com/ Anne E. Carpenter, Ph.D. - MIT ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1153.htm - 7.2KB 74% 08 Aug 07 Find Similar Highlight Purdue Cytometry Mailing List: An innovative new two DVD set on Cytometry- Free to all ... John Wiley & Sons, publisher of Cytometry Part A. These major players in the field of cytometry are known to all of ... Scientific, Coherent, Q-Imaging and Verity Software. All of these companies provide ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0805.htm - 8.7KB 74% 07 Jun 07 Find Similar Highlight Purdue Cytometry Mailing List: RE: Thanks to all for a successful 2007 NW Regional ... ... Purdue Cytometry Mailing List: RE: Thanks to all for a successful 2007 NW Regional ... Miltenyi, Phoenix Flow Systems, Spherotech, Tree Star, Union Biometrica, and Verity Software House, Ryan Brinkman, Perry Halaand, and others of the FICCS ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0471.htm - 6.7KB 74% 28 Mar 07 Find Similar Highlight Purdue Cytometry Mailing List: Applications Specialist - iCyt - ... Northwest Cytometry Users Group and Cascade Cytometry Users Group are pleased to announce the 2007 NW Regional Cytometry Meeting, which ... Tree Star, Union Biometrica, and Verity Software House. ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0333.htm - 7.0KB 74% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: By Thread ... Purdue Cytometry Mailing List: By Thread ... DIVA Software on MAC Intel Computers Pamela Shaw (Wed Dec 13 2006 - 13:25:52 EST) ... http://www.cyto.purdue.edu/hmarchiv/2006/thread.htm - 332.1KB 74% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: RE: Diva 4.0 files ... Purdue Cytometry Mailing List: RE: Diva 4.0 files ... Mark E. Munson Sales Manager Verity Software House, Inc. 45A Augusta Road PO Box 247 Topsham, ME 04086 Phone: 207-729-6767 x191 Fax: 207-729-5443 ... http://www.cyto.purdue.edu/hmarchiv/2004/1443.htm - 6.2KB 74% 20 Aug 04 Find Similar Highlight Purdue Cytometry Mailing List: Free WinList Update Available ... Purdue Cytometry Mailing List: Free WinList Update Available ... Mark E. Munson Sales Manager Verity Software House, Inc. 45A Augusta ... http://www.cyto.purdue.edu/hmarchiv/2003/0582.htm - 4.4KB 74% 02 Apr 03 Find Similar Highlight Purdue Cytometry Mailing List: Extracting data from FACS measurements ... Purdue Cytometry Mailing List: Extracting data from FACS measurements ... Mark E. Munson Sales Manager Verity Software House, Inc. 45A Augusta ... http://www.cyto.purdue.edu/hmarchiv/2003/0491.htm - 6.1KB 74% 18 Mar 03 Find Similar Highlight Purdue Cytometry Mailing List: Course Announcement - 25th ANNUAL COURSE IN FLOW CYTOMETRY ... Purdue Cytometry Mailing List: Course Announcement - 25th ANNUAL COURSE IN FLOW CYTOMETRY ... Mark E Munson Verity Software House, Inc. PO Box 247 ... http://www.cyto.purdue.edu/hmarchiv/2001/2327.htm - 6.4KB 74% 23 Oct 01 Find Similar Highlight about 187243 results found, top 500 sorted by relevance score using date hide summaries group by location prev 31-40 next Purdue Cytometry Mailing List: New Purdue DVD - Cytometry Volume 10 ... Purdue Cytometry Mailing List: New Purdue DVD - Cytometry Volume ... Optical, Macs Miltenyi Biotec, SouthernBiotech, Verity Software Accuri, Chroma, Coherent, Cyopeia, Duke ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1751.htm - 6.9KB 73% 17 Nov 07 Find Similar Highlight Purdue Cytometry Mailing List: New England Cytometry Meeting Registration ... Purdue Cytometry Mailing List: New England Cytometry Meeting Registration ... speakers this year: ... C. Bruce Bagwell, MD, Ph.D. - Verity Software House "Probability State Models: A new paradigm for cytometry analysis ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1382.htm - 9.1KB 73% 18 Sep 07 Find Similar Highlight Purdue Cytometry Mailing List: By Thread ... Purdue Cytometry Mailing List: By Thread ... PML protein Jan Nelson (Wed Dec 16 1998 - 16:56:58 EST) ... Permeabilization and Anti- Coagulants Vincent Falco (Wed Dec 16 1998 - 09:07:16 EST) ... http://www.cyto.purdue.edu/hmarchiv/1998/thread.htm - 474.2KB 73% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: By Thread ... Purdue Cytometry Mailing List: By Thread ... Sales position in Germany Brian Hall (Thu Dec 18 1997 - 12:23:18 EST) ... http://www.cyto.purdue.edu/hmarchiv/1997/thread.htm - 480.6KB 73% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: By Thread ... Purdue Cytometry Mailing List: By Thread ... Summarize "Free software" Dorte Christiansen (Fri Dec 12 2003 - 07:45:44 EST) ... http://www.cyto.purdue.edu/hmarchiv/2003/thread.htm - 369.2KB 73% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: By Thread ... Purdue Cytometry Mailing List: By Thread ... staining DARZYNKIEWICZ ZBIGNIEW (Wed Dec 20 2000 - 18:42:40 EST) ... CD45 expression on ADP-activated murine platelets Gregor Rothe (Thu Dec 21 2000 - 07:29:44 EST) ... http://www.cyto.purdue.edu/hmarchiv/2000/thread.htm - 547.3KB 73% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: By Subject ... Purdue Cytometry Mailing List: By Subject ... 2444 messages sorted by: [ author ] [ date ] [ thread ] [ attachment ] Other mail archives ... http://www.cyto.purdue.edu/hmarchiv/2002/subject.htm - 351.6KB 73% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: By Thread ... Purdue Cytometry Mailing List: By Thread ... is good enough? Uriel TK (Tue Dec 14 2004 - 16:04:58 EST) ... RE: How close compensation is good enough? Gerstein, Rachel (Thu Dec 16 2004 - 07:07:48 EST) ... http://www.cyto.purdue.edu/hmarchiv/2004/thread.htm - 363.4KB 73% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: Applications Specialist - iCyt - ... Northwest Cytometry Users Group and Cascade Cytometry Users Group are pleased to announce the 2007 NW Regional Cytometry Meeting, which ... Tree Star, Union Biometrica, and Verity Software House. ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0234.htm - 6.7KB 73% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: By Date ... Purdue Cytometry Mailing List: By Date ... Re: Cell-Dyn 4000 Howard Shapiro (Wed Dec 18 2002 - 21:51:24 EST) ... pluronic acid g...@biocfarm.unibo.it (Thu Dec 19 2002 - 07:07:54 EST) ... http://www.cyto.purdue.edu/hmarchiv/2002/ - 419.6KB 73% 30 Jan 07 Find Similar Highlight Try this query on the entire Web. score using date hide summaries group by location prev 31-40 next about 187243 results found, top 500 sorted by relevance score using date hide summaries group by location prev 41-50 next Purdue Cytometry Mailing List: By Thread ... Purdue Cytometry Mailing List: By Thread ... free FACS analysis software Wolfraim, Lawrence (NCI) (Wed Dec 19 2001 - 10:28:23 EST) ... http://www.cyto.purdue.edu/hmarchiv/2001/thread.htm - 478.0KB 73% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: By Thread ... Purdue Cytometry Mailing List: By Thread ... WinMDI software - long filename problem WAS Doubts About WinMDI software Alan Bishop (Thu Dec 01 2005 - 19:07:54 EST) ... http://www.cyto.purdue.edu/hmarchiv/2005/thread.htm - 353.3KB 73% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: By Author ... Purdue Cytometry Mailing List: By Author ... 2 flow cytometry job openings at Biogen (Thu May 23 2002 - 07:09:37 EST) ... http://www.cyto.purdue.edu/hmarchiv/2002/author.htm - 360.8KB 73% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: Re: DIVA Software on MAC Intel Co ... Purdue Cytometry Mailing List: Re: DIVA Software on MAC Intel Co ... screen display issue, that made it difficult to read the text. Verity provided us with a patch for this in remarkably little time ... http://www.cyto.purdue.edu/hmarchiv/2006/1979.htm - 6.9KB 73% 15 Dec 06 Find Similar Highlight Purdue Cytometry Mailing List: Re: Log-like transforms ... Purdue Cytometry Mailing List: Re: Log-like transforms ... purposes. > > > > Bruce > > > > C. Bruce Bagwell MD, Ph.D. > > President > > Verity Software House > > 45A Augusta Road > > PO Box 247 > > Topsham, ME 04086 ... http://www.cyto.purdue.edu/hmarchiv/2006/0540.htm - 10.0KB 73% 22 Mar 06 Find Similar Highlight Purdue Cytometry Mailing List: RE: Software for merging FSC 3 fi ... Purdue Cytometry Mailing List: RE: Software for merging FSC ... J. Herbert Technical Support Manager Verity Software House, Inc. PO Box 247 ... http://www.cyto.purdue.edu/hmarchiv/2005/0217.htm - 6.6KB 73% 03 Feb 05 Find Similar Highlight Purdue Cytometry Mailing List: RE: Software recommendations for ... Purdue Cytometry Mailing List: RE: Software recommendations for ... Mark E. Munson Sales Manager Verity Software House, Inc. 45A Augusta ... http://www.cyto.purdue.edu/hmarchiv/2004/1894.htm - 5.9KB 73% 25 Oct 04 Find Similar Highlight Purdue Cytometry Mailing List: Analysis Software ... Purdue Cytometry Mailing List: Analysis Software ... Donald J. Herbert Technical Support Manager Verity Software House, Inc. PO Box 247 45A Augusta Road Topsham, ME, USA 04086 ... http://www.cyto.purdue.edu/hmarchiv/2004/1196.htm - 7.1KB 73% 30 Jun 04 Find Similar Highlight Purdue Cytometry Mailing List: Re: Software to overlay dot plots for analysis ... Purdue Cytometry Mailing List: Re: Software to overlay dot plots for analysis ... Quest for OSX - a new release from BD 4. WinList - Verity Software House 5. FCS Express - from www.denovosoftware.com ... http://www.cyto.purdue.edu/hmarchiv/2003/0979.htm - 5.6KB 73% 28 May 03 Find Similar Highlight Purdue Cytometry Mailing List: RE: Kolmogorov-Smirnov (Is there a better solution?) ... Purdue Cytometry Mailing List: RE: Kolmogorov-Smirnov (Is there ... Bruce Bagwell, MD, Ph.D. Verity Software House, Inc. Published: Clinical Immunology ... http://www.cyto.purdue.edu/hmarchiv/2003/0720.htm - 5.3KB 73% 18 Apr 03 Find Similar Highlight Try this query on the entire Web. score using date hide summaries group by location prev 41-50 next about 187243 results found, top 500 sorted by relevance score using date hide summaries group by location prev 51-60 next Purdue Cytometry Mailing List: Re: non-radioactive LPA and responses to query on LPAs ... PKH Proliferation assays by flow cytometry Organization: Verity Software House ... dartmouth.edu (Alice L. Givan) ModFit software from Verity has a "Proliferation Wizard" that ... http://www.cyto.purdue.edu/hmarchiv/2002/1701.htm - 17.3KB 73% 20 Aug 02 Find Similar Highlight Purdue Cytometry Mailing List: RE: query on LPAs: replacing tritiated thymidine with flow ... Purdue Cytometry Mailing List: RE: query on LPAs: replacing ... J. Herbert Technical Support Manager Verity Software House, Inc. PO Box 247 ... http://www.cyto.purdue.edu/hmarchiv/2002/1649.htm - 9.5KB 73% 13 Aug 02 Find Similar Highlight Purdue Cytometry Mailing List: Re: Last Message on Purdue Cytometry CD- ROM Vol 6 ... Cytometry Mailing List <cytome...@flowcyt.cyto.purdue.edu> ... Associates, Inc; Spherotech, Tree Star, Verity Software, Spherotech, Tree Star, Verity Software. ... http://www.cyto.purdue.edu/hmarchiv/2002/0735.htm - 7.5KB 73% 03 Apr 02 Find Similar Highlight Purdue Cytometry Mailing List: UV excitable dyes for surface phenotyping - ELF-97? ... Purdue Cytometry Mailing List: UV excitable dyes for surface phenotyping - ELF-97? ... RE: Bacteria sorting?" ... Previous message: VSH - Tech Support: "Announcing User Forum at Verity Software House, Inc." ... http://www.cyto.purdue.edu/hmarchiv/2002/0543.htm - 5.8KB 73% 12 Mar 02 Find Similar Highlight Purdue Cytometry Mailing List: 25th ANNUAL COURSE IN FLOW CYTOMETRY: BOWDOIN COLLEGE, ... ... Purdue Cytometry Mailing List: 25th ANNUAL COURSE IN FLOW CYTOMETRY: BOWDOIN COLLEGE, BRUNSWICK MAINE - JUNE 2002 ... Mark E Munson Verity Software House, Inc. PO Box 247 Topsham, ME 04086 Tel: (207) 729-6767 x105 ... http://www.cyto.purdue.edu/hmarchiv/2002/0144.htm - 6.2KB 73% 22 Jan 02 Find Similar Highlight Purdue Cytometry Mailing List: Re: G4's will run OS8.6 (ours sur ... Purdue Cytometry Mailing List: Re: G4's will run OS8.6 (ours sur ... evidenced by the hundreds of labs running their XL software under > DOS (yuk!). Maybe Verity or TreeStar will make some inroads if we demand ... http://www.cyto.purdue.edu/hmarchiv/2000/0156.htm - 8.0KB 73% 13 Jan 00 Find Similar Highlight Purdue Cytometry Mailing List: G4's will run OS8.6 ... Purdue Cytometry Mailing List: G4's will run OS8.6 ... edge software, evidenced by the hundreds of labs running their XL software under DOS (yuk!). Maybe Verity or TreeStar will make some inroads if we demand state of ... http://www.cyto.purdue.edu/hmarchiv/2000/0140.htm - 7.4KB 73% 13 Jan 00 Find Similar Highlight Purdue Cytometry Mailing List: RE: flow cytometry course in June ... Purdue Cytometry Mailing List: RE: flow cytometry course in June ... Brunswick Maine. Course organizer is C. Bruce Bagwell. I think that Verity Software may be sponsoring it, as their information is on some of ... http://www.cyto.purdue.edu/hmarchiv/1998/0905.htm - 5.1KB 73% 10 Apr 98 Find Similar Highlight Purdue Cytometry Mailing List: RE: utility software to convert fcs1.0 to fcs2.0 ... Purdue Cytometry Mailing List: RE: utility software to convert ... PC, I suggest you contact Verity Software (207-729-6767) . I quickly ... http://www.cyto.purdue.edu/hmarchiv/1998/0707.htm - 5.9KB 73% 27 Mar 98 Find Similar Highlight Purdue Cytometry Mailing List: RE: utility software to convert fcs1.0 to fcs2.0 ... Purdue Cytometry Mailing List: RE: utility software to convert fcs1.0 to fcs2.0 ... Since you are using the PC, I suggest you contact Verity Software (207-729-6767) . I quickly tried this function this AM ... http://www.cyto.purdue.edu/hmarchiv/1998/0727.htm - 7.8KB 73% 27 Mar 98 Find Similar Highlight Try this query on the entire Web. score using date hide summaries group by location prev 51-60 next about 187243 results found, top 500 sorted by relevance score using date hide summaries group by location prev 61-70 next Purdue Cytometry Mailing List: FW: FCS file transfer between BD Diva (PC) and CellQuest ( ... ... Purdue Cytometry Mailing List: FW: FCS file transfer between ... morphosys.com> Date: Thu Nov 08 2007 - 07:14:16 EST ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1708.htm - 9.1KB 72% 10 Nov 07 Find Similar Highlight Purdue Cytometry Mailing List: RE: 2008 NW Regional Cytometry Meeting announcement ... Purdue Cytometry Mailing List: RE: 2008 NW Regional Cytometry Meeting announcement ... Bioscience, Miltenyi Biotec, MDA Analytical Technologies, Partec, Tree Star, Union Biometrica, and Verity Software House. ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1644.htm - 6.5KB 72% 03 Nov 07 Find Similar Highlight Purdue Cytometry Mailing List: New England Cytometry Meeting Registration Reminder ... Purdue Cytometry Mailing List: New England Cytometry Meeting Registration Reminder ... speakers this year: ... C. Bruce Bagwell, MD, Ph.D. - Verity Software House "Probability State Models: A new paradigm for cytometry analysis ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1458.htm - 8.6KB 72% 04 Oct 07 Find Similar Highlight Purdue Cytometry Mailing List: New England Cytometry Annual Meeting Registration and Hotel ... ... Purdue Cytometry Mailing List: New England Cytometry Annual Meeting Registration and Hotel Information ... year: ... C. Bruce Bagwell, MD, Ph.D. - Verity Software House "Probability State Models: A new paradigm for cytometry ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1411.htm - 8.8KB 72% 23 Sep 07 Find Similar Highlight Purdue Cytometry Mailing List: SUMMARY of Responses: Biexponential Plots and CSFE ... Purdue Cytometry Mailing List: SUMMARY of Responses: Biexponential Plots and CSFE ... FCS3.0 digital data was presented by Mark Munson of Verity Software House (makers of Modfit). Thanks Mark, it worked great ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1403.htm - 8.1KB 72% 22 Sep 07 Find Similar Highlight Purdue Cytometry Mailing List: Invitation for Vendor Sponsorship for the New England ... ... to announce the New England Cytometry Users Group Fall meeting; Current Concepts in Flow and Image Cytometry which will be held October ... Bruce Bagwell, MD, Ph.D. - Verity Software House http://www.vsh.com ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1175.htm - 8.6KB 72% 11 Aug 07 Find Similar Highlight Purdue Cytometry Mailing List: RE: CFSE graphs (UNCLASSIFIED) ... Verity's MODFIT software has a very nice tool for proliferation ... Stelekati [mailto:e...@fz-borstel.de] >>>Sent: Monday, March 26, 2007 6:58 AM >>>To: Cytometry Mailing List >>>Subject: CFSE graphs >>> >>>Dear all, >>>I want to ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0490.htm - 9.0KB 72% 30 Mar 07 Find Similar Highlight Purdue Cytometry Mailing List: Applications Specialist - iCyt - ... Purdue Cytometry Mailing List: Applications Specialist - iCyt ... Sponsored by: The National Flow Cytometry Resource (NFCR) and Verity Software House ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0214.htm - 8.4KB 72% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: By Date ... Purdue Cytometry Mailing List: By Date ... RE: DIVA SOFTWARE ON INTEL CORE 2 DUO PROCESSOR Guy Hermans ... http://www.cyto.purdue.edu/hmarchiv/2006/ - 367.5KB 72% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: By Author ... Re: Free Flow Cytometry Analysis software? (Fri Apr 14 2006 - 02:56:18 EDT) ... CORRECTION- Chesapeake Cytometry Consortium (Thu Sep 07 2006 - 12:20:05 EDT) ... http://www.cyto.purdue.edu/hmarchiv/2006/author.htm - 299.3KB 72% 30 Jan 07 Find Similar Highlight Try this query on the entire Web. score using date hide summaries group by location prev 61-70 next Purdue Cytometry Mailing List: RE: Partec CyFlow ML? (was Re: Analyzers) ... Purdue Cytometry Mailing List: RE: Partec CyFlow ML? (was Re: Analyzers) ... Received on Fri Nov 30 18:07:07 2007 ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1871.htm - 8.3KB 71% 01 Dec 07 Find Similar Highlight Purdue Cytometry Mailing List: RE: Cytometry software for linux ... Purdue Cytometry Mailing List: RE: Cytometry software for linux ... ugr.es] > Sent: Monday, September 10, 2007 12:28 PM > To: Cytometry Mailing List > Subject: Cytometry software ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1361.htm - 7.1KB 71% 15 Sep 07 Find Similar Highlight Purdue Cytometry Mailing List: Re: Summary and replies on PCH101 ... Purdue Cytometry Mailing List: Re: Summary and replies on PCH101 ... From: clare Rogers <rog...@med.umich.edu> Date: Fri Sep 07 2007 - 17:04:21 EDT ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1350.htm - 13.3KB 71% 11 Sep 07 Find Similar Highlight Purdue Cytometry Mailing List: For Sale BD FACSVantage with DiVa option ... Purdue Cytometry Mailing List: For Sale BD FACSVantage with DiVa option ... ml tubes -- PC and Mac computers with all original BD software (DiVa [PC] and CELLQuest [Mac.]) NOTE: I will only guarantee ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1306.htm - 7.6KB 71% 01 Sep 07 Find Similar Highlight Purdue Cytometry Mailing List: BD bead array software and FC500s ... Purdue Cytometry Mailing List: BD bead array software and FC500s ... From: Stephen Taylor - Porton Down <Stephen.Tay...@hpa.org.uk> Date: Thu Aug 16 2007 - 07:01:09 EDT ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1204.htm - 6.0KB 71% 18 Aug 07 Find Similar Highlight Purdue Cytometry Mailing List: RE: Upgrading Computers and BD Software ... Purdue Cytometry Mailing List: RE: Upgrading Computers and BD Software ... utoronto.ca> > To: Cytometry Mailing List <cytome...@flowcyt.cyto.purdue.edu> > Date: 8/13/2007 ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1203.htm - 8.1KB 71% 17 Aug 07 Find Similar about 187242 results found, top 500 sorted by relevance score using date hide summaries group by location prev 91-100 next Purdue Cytometry Mailing List: FACSort for sale. ... Purdue Cytometry Mailing List: FACSort for sale. ... Blue laser and sorting option (bought in Oct. 1997) with Cellquest software installed in Power Macintosh computer. The laser current is around 5.70Amps ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0616.htm - 5.6KB 71% 02 May 07 Find Similar Highlight Purdue Cytometry Mailing List: By Date ... Purdue Cytometry Mailing List: By Date ... Summarize "Free software" Dorte Christiansen (Fri Dec 12 2003 - 07:45:44 EST) ... http://www.cyto.purdue.edu/hmarchiv/2003/ - 385.3KB 71% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: By Author ... Purdue Cytometry Mailing List: By Author ... Flow facility, again (Fri Dec 04 1998 - 03:50:07 EST) ... Flow facility (Mon Nov 23 1998 - 07:07:29 EST) ... http://www.cyto.purdue.edu/hmarchiv/1998/author.htm - 416.0KB 71% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: By Date ... Purdue Cytometry Mailing List: By Date ... PML protein Jan Nelson (Wed Dec 16 1998 - 16:56:58 EST) ... Permeabilization and Anti- Coagulants Vincent Falco (Wed Dec 16 1998 - 09:07:16 EST) ... http://www.cyto.purdue.edu/hmarchiv/1998/index.htm - 491.8KB 71% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: By Subject ... Purdue Cytometry Mailing List: By Subject ... owner-cytometry (Wed Jun 07 2006 - 10:19:50 EDT) ... http://www.cyto.purdue.edu/hmarchiv/2006/subject.htm - 293.3KB 71% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: By Subject ... Purdue Cytometry Mailing List: By Subject ... Rosie Clarke (Fri Feb 07 2003 - 07:14:11 EST) ... http://www.cyto.purdue.edu/hmarchiv/2003/subject.htm - 324.3KB 71% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: By Author ... Re: Réf. : Free software? (Fri Dec 19 2003 - 22:39:46 EST) ... UV vs Krypton UV (Thu Feb 20 2003 - 19:09:39 EST) ... RE: malignant plasma cell detection by flow cytometry (Fri Feb 07 2003 - 19:48:43 EST) ... http://www.cyto.purdue.edu/hmarchiv/2003/author.htm - 331.6KB 71% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: By Date ... Purdue Cytometry Mailing List: By Date ... Thu Dec 16 2004 - 07:07:48 EST ... http://www.cyto.purdue.edu/hmarchiv/2004/ - 402.2KB 71% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: By Author ... Purdue Cytometry Mailing List: By Author ... Re: OS9+Cellquest (Tue Mar 07 2000 - 08:07:34 EST) ... http://www.cyto.purdue.edu/hmarchiv/2000/author.htm - 484.2KB 71% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: By Date ... Purdue Cytometry Mailing List: By Date ... Thu Dec 21 2000 - 07:29:44 EST ... http://www.cyto.purdue.edu/hmarchiv/2000/index.htm - 601.9KB 71% 30 Jan 07 Find Similar Highlight Try this query on the entire Web. score using date hide summaries group by location prev 91-100 next about 187242 results found, top 500 sorted by relevance score using date hide summaries group by location prev 101-110 next Purdue Cytometry Mailing List: By Subject ... Purdue Cytometry Mailing List: By Subject ... JSC-SD) (Wed Sep 06 2000 - 08:37:10 EST) ... Walker, Don (SEA) (Thu Aug 31 2000 - 14:09:16 EST) ... Palkowetz, Kimberly H. (Tue Aug 29 2000 - 07:49:22 EST) ... http://www.cyto.purdue.edu/hmarchiv/2000/subject.htm - 483.5KB 71% 30 Jan 07 Find Similar Highlight Purdue Cytometry Mailing List: By Date ... Purdue Cytometry Mailing List: By Date ... Sales position in Germany Brian Hall (Thu Dec 18 1997 - 12:23:18 EST) ... http://www.cyto.purdue.edu/hmarchiv/1997/index.htm - 498.6KB 71% 30 Jan 07 Find Similar Highlight Purdue |
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