Purdue University Mailing List Service Terms of Use
Purdue University provides the Mailing List Service for activities
and
associated administrative functions supporting its mission of
learning, discovery, and engagement.  Use of the Mailing List Service
is subject to Purdue University policies, in particular the policies
described in "Use of Electronic Mail" and "IT Resource Acceptable Use
Policy".
Acceptable Use:
The Mailing List Service is to be used for University-related
educational, research and administrative purposes. Any use of the
Mailing List Service that interferes with University activities and
functions or does not respect the image and reputation of Purdue
University is improper.
It is the subscriber's responsibility to determine the purpose of a
Purdue University mailing list before subscribing or sending messages
to the list. Persons subscribed to a Purdue University mailing list
will be viewed as having solicited any material delivered by the
list,
as long as that material is consistent with the purpose of the list.
Access
Purdue University mailing lists may be established by Purdue faculty,
students, staff and other individual and entities granted official
Purdue e-mail privileges at Purdue University.  Access to individual
mailing lists is controlled by the individual list owners.
All Purdue University mailing lists must have at least one owner who
has a valid and active official Purdue University e-mail account of
the form usern...@purdue.edu.  Any list that does not have a valid
and
active Purdue owner may be removed from the system.  Warning will be
given to list owners and list members before such lists are
discontinued.
It is recommended that Purdue University mailing list administrator
and subscriber passwords be different from Purdue Career Account
passwords and any other passwords.
Consequences of Illegal or Improper Actions
Actions that are illegal or that are against Purdue University policy
will be referred to the appropriate officials.
Actions that are in violation of the Terms of Use will result in the
blocking of posts from offending subscribers, removal of offending
subscribers from lists or the removal of lists.
The Mailing List Service administrators may monitor user activities
and access any of the service's files or information in the course of
performing normal system maintenance or while investigating policy
violations or improper use. Anyone using Purdue University mailing
list resources expressly consents to such monitoring and is advised
that if such monitoring reveals possible evidence of criminal
activity, Purdue will provide the evidence to law enforcement
officials.
Additional Improper Actions
In addition to any actions prohibited by federal or state laws and
policies, or by Purdue University policies, the following specific
actions and uses of Purdue University Mailing List Facilities are
improper:
Concealing or misrepresenting names or affiliations in Purdue
University mailing list names.
Using Purdue University mailing lists for organized political
activity
or political solicitation.
Sending any e-mail to a Purdue University mailing list that is not
consistent with the purpose of the list. Messages not relevant to the
purpose of the list will be viewed as unsolicited e-mail.
Continuing to send e-mail to a Purdue University mailing list after
the list owner has requested that the user stop sending to the list
because they are not following the guidelines or topic established
for
the list.
Harvesting e-mail addresses from another mailing list in order to
establish a Purdue University mailing list. It is permissible to post
a message to a closely related list to invite people to subscribe to
another Purdue University mailing list.
Harvesting e-mail addresses without proper authority from an
institution's directory, password file or any other source to
establish a Purdue University mailing list.
Harvesting e-mail addresses from a Purdue University mailing list for
use other than the intended purpose of the mailing list.
Subscribing any e-mail address to a Purdue University mailing list
except with proper authority or the individual's permission.
List Service Administration
Lists that are determined to not be active for extended periods will
be subject to removal.  Warning will be given to list owners and list
members before such lists are discontinued.
The Mailing List Administrators reserve the right to trim archives
for
lists with archives that grow too large.  A message will be sent to
list owners before trimming such archives.
This Terms of Use statement is subject to modification.  While a
message will be sent to list owners if major changes are necessary,
all users of the Mailing List Service should regularly verify that
their use of the service conforms to the policies in the current
Terms
of Use statement.
*************************************************************************************TALK
ABOUT PRESIDENT  Geroge W. Bush and John F Kerry **YOUR  POLITICAL
CHAT  LINE**FLOW JO MARKETING.*******MARIO ROEDERER
mac vs. pc
*       This message: [ Message body ] [ More options ]
*       Related messages: [ Next message ] [ Previous message ] [ In
reply
to ] [ Next in thread ] [ Replies ]
From: Mario Roederer <roede...@drmr.com>
Date: Wed Aug 25 2004 - 15:10:25 EST
I have only one comment to make on this debate:
George W. Bush ("nuculer") uses a PC.
John F. Kerry uses a Mac (<http://www.macminute.com/2004/07/14/
kerry>).
Hmm.
mr
Received on Thu Aug 26 13:18:00 2004
*       This message: [ Message body ]
*       Next message: Bjvrnsson Sven G: "Cell cycle analysis with
DRAQ5"
*       Previous message: WThrond...@genencor.com: "Re: dr. mr. on
Apple"
*       In reply to: Robin Barclay: "Re: mr on Apple web site"
*       Next in thread: Hirschkorn, Dale: "RE: mac vs. pc"
*       Maybe reply: Hirschkorn, Dale: "RE: mac vs. pc"
*       Maybe reply: Konz, Ric
hard: "RE: mac vs. pc"
*       Reply: Joanne Lannigan: "RE: mac vs. pc"
*       Reply: Robert C. Leif: "RE: mac vs. pc"
*       Reply: Kristie Gordon: "Re: mac vs. pc"
*       Maybe reply: Vandevert, Chris: "RE: mac vs. pc"
*       Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
Subject ] [ By Author ] [ By messages with attachments ]
This archive was generated by hypermail 2.1.8 : Tue Aug 31 2004 -
03:12:05 EST

**************************************************************************************COMMERCIAL
MARKETING THROUGH LIST BLATENT      VERITY HOUSE SOFTWARE
Analysis Software
This message: [ Message body ] [ More options ]
Related messages: [ Next message ] [ Previous message ]
From: VSH - Tech Support <t...@vsh.com>
Date: Wed Jun 30 2004 - 12:08:14 EST
David
At the risk of sounding commercial, WinList can do what you want.  I
have
attached a few pictures showing the WinList Region Array feature.
With this
you can tell the program exactly how you want the regions created and
how
many.  If you would like to try it for yourself you can download a
trial
version from our web site www.vsh.com.
Please let me know if you have any questions.
Best regards
Don
Donald J. Herbert
Technical Support Manager
Verity Software House, Inc.
PO Box 247
45A Augusta Road
Topsham, ME, USA  04086
Phone: (207) 729-6767 ext.190
Fax:   (207) 729-5443
email:  t...@vsh.com
web: www.vsh.com
Verity Software House Has a Connected Search Engine attached selling
all software.

WHY DOES VERITY REQUEST ARTICLES TO BE DELETED AFTER READING?

WHY MAIL LIST IS DOWN THESE LINK ARE NOT ACTIVE

**************************************************************************************
Purdue Cytometry Mailing List: Re: DNA analysis software
... J. Herbert > Technical Support Manager > Verity Software
House ...
sender immediately by return e-mail, delete this e-mail and destroy
any copies. Any ...
http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0755.htm - 8.6KB  70%

26 May 07
Find Similar
Highlight
---------------------------------------------------------------------------
¬-----
Purdue Cytometry Mailing List: DNA analysis software
... a conversion at no charge. I invite you to contact Verity
Software
House directly with any specific questions or issues. Best
regards ...
issues. Best regards, Don Donald J. Herbert Technical Support Manager
Verity Software House ...
http://www.cyto.purdue.edu/hmarchiv/Current/0753.htm - 5.6KB  69%

26 May 07
Find Similar
Highlight
---------------------------------------------------------------------------
¬-----
Purdue Cytometry Mailing List: Re: DNA analysis software
... J. Herbert > Technical Support Manager > Verity Software
House ...
sender immediately by return e-mail, delete this e-mail and destroy
any copies. Any ...
http://www.cyto.purdue.edu/hmarchiv/Current/0755.htm - 8.6KB  69%

26 May 07
Find Similar
Highlight
---------------------------------------------------------------------------
¬-----
Purdue Cytometry Mailing List: RE: DNA analysis software
... Purdue Cytometry Mailing List: RE: DNA analysis software ... J.
Herbert Technical Support Manager Verity Software House ...
http://www.cyto.purdue.edu/hmarchiv/Current/0747.htm - 7.7KB  69%

26 May 07
Find Similar
Highlight
---------------------------------------------------------------------------
¬-----
Purdue Cytometry Mailing List: RE: DNA analysis software
... Purdue Cytometry Mailing List: RE: DNA analysis software ... J.
Herbert Technical Support Manager Verity Software House ...
http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0747.htm - 7.7KB  68%

26 May 07
Find Similar
Highlight
---------------------------------------------------------------------------
¬-----
Purdue Cytometry Mailing List: RE: FL2H in PI cell cycle analysis
... From: Verity Software House <ver...@vsh.com> Date: Fri Sep 07
2007
- 17:30:59 EDT ... Verity Software House ...
http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1351.htm - 7.2KB  66%

12 Sep 07
Find Similar
Highlight
ANTI Commercial posting **NOTICE HOW THEY ADD A SMILEY
FACE**********FCS PRESS RAY HICKS
**************************************************************************************
anti) Commercial Posting:)
This message: [ Message body ] [ More options ]
Related messages: [ Next message ] [ Previous message ]
From: Ray Hicks <rh...@cam.ac.uk>
Date: Wed Oct 13 2004 - 08:43:13 EST
Just a quick nopte to let people know that FCSPress is now shareware
(no time restrictions or feature reductions for free use, though if
you feel like like paying for it you can using paypal). It also now
works with Diva export files and runs ok under classic on OS 10.2 and
10.3.  I've discontinued FCS Assistant, which has fallen behind
FCSPress remarkably, and which failed to run as shareware on some
systems (it insisted you buy it or refused to run - FCSPress doesn't
even have a nag screen now - pesky things).
FCSPress is available from www.fcspress.com
Enjoy!
Ray
Received on Wed Oct 13 14:38:00 2004
This message: [ Message body ]
Next message: Mike: "Re: (almost) sterile sorting"
Previous message: Hans-Georg.Krey...@merck.de: "Once more: FACSstar
plus dual laser to give away (incl. location of the instrument)"
Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
Subject ]
[ By Author ] [ By messages with attachments ]
This archive was generated by hypermail 2.1.8 : Thu Oct 14 2004 -
03:12:04 EST
Mr. Apple WEB IS Mario OWNER OF FLOW JO **MORE MARKETING
**************************************************************************************
Re: mr on Apple web site
*       This message: [ Message body ] [ More options ]
*       Related messages: [ Next message ] [ Previous message ] [ In
reply
to ] [ Next in thread ] [ Replies ]
From: Robin Barclay <Robin.Barc...@ed.ac.uk>
Date: Wed Aug 25 2004 - 04:17:52 EST
Well .... some of us PC afficianados hate Macs just as much -
personally I
have hated them since they first made it difficult to access their
DOS
and
write programs in any language (especially for accessing/controlling
lab
apparatus) - back in the '70's when there were several different
options -
not just PC's.  I am glad that the "PC" became a standard (there were
too
many diferent systems) and that IBM did not hang on to it the way
Apple kept
the Mac in house (you get more for your money with a PC because many
different people make them).  You don't often get PC people knocking
Macs
the way that the Mac people knock PCs - there seems to be a lot of
Microsoft
paranoia.  In my opinion PCs are much more common and versatile in
labs than
Macs - especially outside the USA - and will eventually become the
standard
for interfacing with lab equipment..... and they can "look cool" if
you shop
around for the right case if that's important to you.
Robin Barclay
Beverly Barton **STEERING COMMITY FOR* SCIENCE ADVISORY BOARD*****
Kanecki Associates Inc Discussion of FCS QUICK FACS 11 DELETED OFF THE
SITE!
MORE FLOW JO
**************************************************************************************
----- Original Message -----
From: "Beverly Barton" <barto...@UMDNJ.EDU>
To: cyto-inbox
Sent: Monday, August 23, 2004 10:09 PM
Subject: Re: mr on Apple web site

Received on Wed Aug 25 13:58:00 2004
*       This message: [ Message body ]
*       Next message: WThrond...@genencor.com: "Re: dr. mr. on Apple"
*       Previous message: facs_copy: "Re: Sorting issues"
*       In reply to: Beverly Barton: "Re: mr on Apple web site"
*       Next in thread: Mario Roederer: "mac vs. pc"
*       Reply: Mario Roederer: "mac vs. pc"
*       Reply: Howard Shapiro: "Re: mr on Apple web site"
*       Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
Subject ] [ By Author ] [ By messages with attachments ]
This archive was generated by hypermail 2.1.8 : Fri Aug 27 2004 -
03:12:05 EST

***THE MENTION OF BD CYTOMETERS AND MORE MARKETING FOR FLOW JO **
MARIO

Randy T. Fischer**********
NIH/NIAMS

Mr. APPLE WEB SITE FLOW JO
**************************************************************************************
RE: mr on Apple web site
*       This message: [ Message body ] [ More options ]
*       Related messages: [ Next message ] [ Previous message ]
[ Maybe in
reply to ]
From: Fischer, Randy (NIH/NIAMS) <fisch...@mail.nih.gov>
Date: Mon Aug 23 2004 - 16:18:55 EST
Paul,
I know that for a long time, anyone who used a BD flow cytometer had
***************
little
choice but to use a MAC-data acquisition required it.  And, one of
the
best
third party software packages was FlowJo which only ran on a MAC.
*******************
Mario,
being a FlowJock, naturally was heavily exposed to this atmosphere.
However, most of our other laboratory instrumentation appears to run
on the
IBM clone type of PC-ELISA reader, luminometer, ELISpot reader, and
AFFYMETRIX machine to name the first ones that come to mind.  Of
course,
because Flow is such a key component and FlowJo works so well, we
have
MACs
(and associated cinema displays) at all of our desks.  So, Mario is
probably
pretty close as I guess we now run about 50% MAC, and for most things
they
do run better than our PCs for similar applications, but mostly the
two
platforms tend to be used for very disparate applications and we need
both.
Wish more science companies would move to MAC, but as Microsoft
probably
owns a minority share in most companies, that will not likely happen
in our
lifetimes.
Haven't read the article yet, but hopefully Mario remembered to cite
you for
all the work on the Flow website/mailing list.
Randy T. Fischer
NIH/NIAMS
Building 10, Room 6D50
9000 Rockville Pike
Bethesda, MD 20892
(301) 594-3537
fisch...@mail.nih.gov

Received on Tue Aug 24 16:18:00 2004
*       This message: [ Message body ]
*       Next message: Hans Jürgen Hoffmann: "SV: Any suggestion for
selection of ICAM-1 (CD54) antibody?"
*       Previous message: J. Paul Robinson: "Re: mr on Apple web
site"
*       Maybe in reply to: Prussin, Calman (NIH/NIAID): "mr on Apple
web
site"
*       Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
Subject ] [ By Author ] [ By messages with attachments ]
This archive was generated by hypermail 2.1.8 : Wed Aug 25 2004 -
03:12:04 EST

PRISIDENT OF ISAC AND HEAD OF PURDUE CYTOMETRY DEPT J PAUL ROBINSON
PROVIDES MANY GRANTS TO TREESTAR. GRANTS INFORMATION WILL BE PROVIDED
AT END…
****READ DO NOT WET YOUR POCKET PROTECTORS***ADDED LINKS TO WEBSITES
FOR FLOW JO

J.Paul Robinson, PhD                PH:(765)4940757

Received on Tue Aug 24 16:18:00 2004
*       This message: [ Message body ]
*       Next message: Hans Jürgen Hoffmann: "SV: Any suggestion for
selection of ICAM-1 (CD54) antibody?"
*       Previous message: J. Paul Robinson: "Re: mr on Apple web
site"
*       Maybe in reply to: Prussin, Calman (NIH/NIAID): "mr on Apple
web
site"
*       Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
Subject ] [ By Author ] [ By messages with attachments ]
This archive was generated by hypermail 2.1.8 : Wed Aug 25 2004 -
03:12:04 EST
***************************************************************************-
******************************************
I know that for a long time, anyone who used a BD flow cytometer had
little
choice but to use a MAC-data acquisition required it.  And, one of
the
best
third party software packages was FlowJo which only ran on a MAC.
Mario,
being a FlowJock, naturally was heavily exposed to this atmosphere
***************************************************************************-
***************************************
For all of the mr groupies out there in cytometry cyberspace. Don't
wet

***************************************************************************-
********************************************

***************************************************************************-
**********************************************
CYTOMETRY SOFTWARE***PURDUE**Links to flow cytometry sites in the USA
and Canada (home of Mario Roederer and Steve Perfetto) home of J.
Paul
Robinson and ...
Perfetto, Steve (NIH/VRC) <perfe...@mail.nih.gov>
***************************************************************************¬*
GRANTS TROUGH *****PURDUE AND THE GOV. NIH FOR FLOW JO By J.Paul
Robinson
PRESIDENT OF ISAC 2007 -2008 AND BOARD MEMBER FOR A LONG TIME********
The Daily Dongle: - 2 visits - Nov 25
Rant about FlowJo's goings-on. ... NIH Funding. Another thread from
Purdue worth posting: .... I saw this question on Purdue mailing list
earlier today. ...
flowjo.typepad.com/the_daily_dongle/2007/06/index.html - 33k - Cached
- Similar pages - Note this
The Daily Dongle: Current Affairs
Rant about FlowJo's goings-on. ... NIH Funding. Another thread from
Purdue worth posting:. We are calling upon you again during this
Independence Day ...
flowjo.typepad.com/the_daily_dongle/current_affairs/index.html - 27k
-
Cached - Similar pages - Note this
[ More results from flowjo.typepad.com ]
This page under construction
http://www.cyto.purdue.edu/hmarchiv/cytomail.htm. Flow cytometry
analysis software: ... http://www.niaid.nih.gov/reposit/tetramer/index.html.
Ebioscience ...
www.immunology.utoronto.ca/research/resources/msb/fc.htm - 27k -
Cached - Similar pages - Note this
The Daily Dongle: - 2 visits - Nov 25Rant about FlowJo's goings-
on. ... -the impact of cuts to grants already funded .... I saw this
question on Purdue mailing list earlier today. Since FlowJo ...
flowjo.typepad.com/the_daily_dongle/2007/06/index.html - 33k - Cached
- Similar pages - Note this
The Daily Dongle: Current Affairs - 12:10amRant about FlowJo's
goings-
on. ... For those of you not on Purdue, Joao Loureiro from Portugal
wrote: ... -the impact of cuts to grants already funded ...
flowjo.typepad.com/the_daily_dongle/current_affairs/index.html - 27k
-
Cached - Similar pages - Note this
Purdue Cytometry Mailing List: By Date - Nov 25Re: Physical
exhaustion
and immune system FlowJo Technical Support - Maciej Simm (Wed Mar 14
2007 - 17:41:46 ..... Travel grants for young investigators, ...
www.cyto.purdue.edu/hmarchiv/Current/thread.htm - 288k - Cached -
Similar pages - Note this
Purdue Cytometry Mailing List: By Date - 2 visits - Nov 25Re: Using
DIVA Exp Files in Flojo FlowJo Technical Support - Maciej Simm ......
Travel grants for young investigators, ImmunoRio2007
gharago...@sums.ac.ir ...
www.cyto.purdue.edu/hmarchiv/current/index.htm - 318k - Cached -
Similar pages - Note this
[ More results from www.cyto.purdue.edu ]
The Whitaker Foundation 1996 Annual Report - Grants ApprovedRESEARCH
GRANTS Auburn University Thomas S. Denney, Jr., .... State
University)
Becky Jo Ficek Rice University (B.S. Purdue University) Anne Marie
Findlay ...
www.bmes.org/WhitakerArchives/96_annual_report/grantsap.html - 33k -
Cached - Similar pages - Note this
Vision now reality in Purdue's Discovery ParkThe park's Birck Center
has awarded $2.4 million in start-up grants for nine of those new
faculty .... V. Jo Davisson, (7865) 494-5238, davis...@purdue.edu ...
www.purdue.edu/UNS/html3month/2004/040919.Rutledge.dispark.html - 28k
- Cached - Similar pages - Note this
Testimony of JO ANNE GOODNIGHTThe development of the miniaturized
flow
probe marks the first time that anyone has ... Funding provided by an
STTR Phase I grant funding resulted in the ...
www.hhs.gov/asl/testify/t010621.html - 26k - Cached - Similar pages -
Note this
This page under constructionFlow cytometry users: useful sites.
Scripps Research Institute Flow Cytometry Core Facility http://facs.scripps.edu/.
Purdue University Cytometry ...
www.immunology.utoronto.ca/research/resources/msb/fc.htm - 27k -
Cached - Similar pages - Note this
FACS Flow Cytometry FacilityUse of stand-alone workstation with
CellQuest and FlowJo software for data analysis ... Flow cytometry
users: useful sites http://facs.scripps.edu/. Purdue ...
www.immunology.utoronto.ca/CellSorter.htm - 23k - Cached - Similar
pages - Note this
ISAC Homepage - ISAC E-News -- June, 2007 - 15 visits - Dec 9
Best wishes. J. Paul Robinson, President ..... Adam Treister
introduced possibilities of integrating these with FlowJo. ...
www.isac-net.org/content/view/593/119/ - 53k - Cached - Similar pages
THE PAGE ABOVE IS NOT ACTIVE DUE TO INVESTIGATION OF PURDUE CYTOMETRY
MAIL LIST (LINK)
**********************************************************************************************************

NON READABLE FCS 3 FILES COULTER FC 500 Flow Jo Discussion on Software
ERRORS and QUOTING FROM PAUL J ROBINSON HE DOES NOT KNOW IF THEY ARE
GOING TO FIX THE PROBLEM OVER THE MAIL LIST>>  FLOW JO DISCUSSES
****NEW BUILD OF SOFTWARE FOR CYTOMETRS
DOES COULTER KNOW? THIS IS COULTERS SOFTWARE USED IN THE FC 500
CYTOMETER?
************************************************************************************

RE: Nonreadable FCS 3 files from Coulter FC 500 cytometer
*       This message: [ Message body ] [ More options ]
*       Related messages: [ Next message ] [ Previous message ] [ In
reply
to ]
From: J. Paul Robinson <j...@flowcyt.cyto.purdue.edu>
Date: Fri Jun 04 2004 - 21:02:48 EST
We notified Coulter of the error in their software a
long time ago. I dont know if htey are going to fix it.
regards
paul robinson
On 3 Jun 2004 at 16:55, maciej simm wrote:

- Hide quoted text -

J.Paul Robinson, PhD             PH:(765)4940757 ***********
Professor of Immunopharmacology
Professor of Biomedical Engineering
Purdue University          FAX:(765)4940517
EMAIL:j...@flowcyt.cyto.purdue.edu
WEB: http://www.cyto.purdue.edu
Have you seen our new HCS webpage? ***************
http://www.cyto.purdue.edu/hcs
Received on Mon Jun 7 14:18:00 2004
*       This message: [ Message body ]
*       Next message: Donnenberg, Albert: "RE: Nonreadable FCS 3 files
from
Coulter FC 500 cytometer"
*       Previous message: Michael Ormerod: "Flow Cytometry Course"
*       In reply to: maciej simm: "RE: Nonreadable FCS 3 files from
Coulter
FC 500 cytometer"
*       Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
Subject ] [ By Author ] [ By messages with attachments ]
This archive was generated by hypermail 2.1.8 : Tue Jun 08 2004 -
03:12:04 EST
                        FLOW JO AND MARKET SHARE DISCUSSION VS BD
MARKET SHARE
*****************************MARKET SHARE DISCUSSION DOES BD KNOW?
***********
************************************************************************************************************************
Of course, what started this thread was the assertion in the piece
about
Mario on Apple's web site that there were 15,000 to 20,000 Mac-based
flow
cytometers out there. The best estimates I had from industry people
in
early 2003 when I was finishing up the 4th Edition of PFC was that
the
total number of systems from all manufacturers in use was under
20,000. If
that's correct, BD would need a 75% market share to account for the
low end
estimate of 15,000 Mac-based machines. I'd be interested to know
where
the
15,000 to 20,000 figure came from.
***************************************************************************¬***********************************************
Re: mr on Apple web site
*       This message: [ Message body ] [ More options ]
*       Related messages: [ Next message ] [ Previous message ] [ In
reply
to ] [ Next in thread ] [ Replies ]
From: Howard Shapiro <h...@shapirolab.com>
Date: Wed Aug 25 2004 - 18:15:38 EST
Robin Barclay wrote:

I can't pass up the opportunity to get into the PC/Mac battle - I
hate
them
both, but there aren't really alternatives (yes, I know, there's
Linux, but
it's probably even harder to hook up a Linux system to hardware than
it is
to hook a Mac up to hardware).
Of course, what started this thread was the assertion in the piece
about
Mario on Apple's web site that there were 15,000 to 20,000 Mac-based
flow
cytometers out there. The best estimates I had from industry people
in
early 2003 when I was finishing up the 4th Edition of PFC was that
the
total number of systems from all manufacturers in use was under
20,000. If
that's correct, BD would need a 75% market share to account for the
low end
estimate of 15,000 Mac-based machines. I'd be interested to know
where
the
15,000 to 20,000 figure came from.
The old (68000 series and possibly early PowerPC) Macs were
difficult,
but
not impossible to connect to hardware; it was easier to work with the
PC's
ISA bus, which, while slow, was perfectly adequate to do most flow
cytometric data acquisition and analysis. There were decent versions
of
Forth, which was one of the first and best languages designed for
controlling hardware from mini- and microcomputers, for both PC (DOS)
and
the Mac (Forth was the first Mac programming language made
commercially
available, at a time when the only other option was buying the very
expensive Lisa from Apple on which to develop Mac software). I found
MacForth easier to program with than the Windows Forths (or other
Windows
languages, e.g., Delphi), but my old Macs used to crash all the time.
So what makes me unhappy with both Windows [and Windows machines] and
the
Mac in their current incarnations? It is now significantly harder for
mere
mortals to write software to get hardware to communicate with either
PCs or
Macs; the gain in complexity associated with the PCI bus, USB/USB2,
FireWire(IEEE 1394), etc. is greater than the gain in speed and
convenience. Also, in making the operating systems more stable (and
Win XP,
despite its security issues, is almost as stable as Mac's Unix-based
OS X),
both Microsoft and Apple elected to eliminate the ability of their
computer
hardware to respond rapidly to interrupts (latencies are now in the
tens of
thousands of instruction cycles), meaning that any really fast
hardware
attachment for either a PC or a Mac now needs to have a DSP in it,
whereas
if the fast interrupt response had been preserved, the hardware
attachments
could have been made much simpler and cheaper. Linux also takes fast
interrupt response off the table, so it doesn't represent a viable
alternative. If you go to Apple's web site and look at what data
acquisition hardware is available for Macs, particularly for Macs
running
OS X, there isn't much, and many of the companies that supported the
Mac in
that area have dropped Mac support for their newer products. That is
undoubtedly one big reason why BD's newer digital pulse processing
cytometers are running on the PC platform.
That doesn't stop anybody from analyzing FCS files on Macs. FlowJo is
well-conceived flow cytometry software; one reason it is as good as
it
is
is that it was written by people who do a lot of flow cytometry, and
cutting edge flow cytometry at that. But there are other people who
do
a
lot of good flow cytometry who have written good software, for PCs as
well
as for Macs.
For the record, I have a G4 PowerBook, which I use mostly for the
iLife
applications, which are slick. It doesn't crash more than once every
couple
of months, but the same is true of my Windows 2000 and XP systems. OS
X can
be as infuriating as Windows when one or another aspect of it goes
counter
to your intuition or to what you have gotten used to. Macs, while
somewhat
more expensive, are much better made than many PCs, and they are
certainly
aesthetically pleasing. If there were a reasonable alternative to
Microsoft's Office applications for the Mac, I might consider
switching.
The 12.1" PowerBook is a nice portable, but it's over a pound heavier
than
the Fujitsu laptop I now use, which has pretty much the same speed,
memory,
and drive capabilities (OK, not DVD-R, but I don't burn a lot of
DVDs).
However, I really wish Apple had stuck with the plan they had a few
years
back of writing a Mac operating system for Intel hardware. I think
that
died when Microsoft bailed Apple out with a few hundred mil. If it
were
possible to run OS X and XP mano a mano on the same hardware, there'd
be a
more rational basis for comparison. But, as may be the case for the
November election, minds, once made up, are not easily changed.
-Howard
Received on Thu Aug 26 16:38:00 2004
*       This message: [ Message body ]
*       Next message: Konz, Richard: "Discrimination of GO/G1"
*       Previous message: Ann Atzberger: "RE: optimum concentration
for
sorting"
*       In reply to: Robin Barclay: "Re: mr on Apple web site"
*       Next in thread: Robert C. Leif: "RE: mr on Apple web site"
*       Reply: Robert C. Leif: "RE: mr on Apple web site"
*       Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
Subject ] [ By Author ] [ By messages with attachments ]
This archive was generated by hypermail 2.1.8 : Sat Aug 28 2004 -
03:12:06 EST
HOW TO DEVELOP SOFTWARE? YOU CAN USE EXISTING COMMERCIAL PROGRAMS?
RESPONSE WITH FLOW JO ***ADAM TRIESTER    TREESTAR/FLOW JO
************************************************************************************************************************
You can use existing commercial programs (Aabel & JMP are nice
Mac 3D applications, and I'm sure there are others), or write it
yourself in OpenGL.  I think OpenGL would be a better choice than C+
+.
OpenGL is a higher level graphics language, and knows how to access
the
specialized accelerators in the graphics cards, which are actually
faster for this stuff than the G5.  That's how the dungeon games do
the
3D shading and rendering.
I'll be happy to donate a considerable amount of code that I've done
in
this effort (most was taken from an old freeware program called
Rotator, which I no longer could find with Google, but I have
somewhere
in my archives), but we decided this was pretty much a dead end.
Rotator was in C, and quite unreadable. For the investment this task
would take, I think it'd be better to start over in OpenGL.  It's
cross-platform too, which is important, as you'll find you want to
port
it to a Cray.
I'd still think you want to use FlowJo to read the DiVa files,
compensate them, transform them, gate them, and then export desired
subpopulations to the 3D viewer.   If it were any other instrument,
you
could probably read the files yourself, with R or our free Java
libraries, but the DiVa files  are a unique format, and almost always
require compensation and transformation to a lin/log scale, so
there's
a lot of work before you even get to viewing them.
***************************************************************************-
***********************************************
Re: 3D Graphics for flow data display
This message: [ Message body ] [ More options ]
Related messages: [ Next message ] [ Previous message ] [ Maybe in
reply to ] [ Next in thread ]
From: Adam Treister <a...@treestar.com>
Date: Sun Jan 04 2004 - 13:48:24 EST
David,
We've tried on a couple of occasions to add a "spatial" 3D module to
FlowJo, and it has never turned out well enough to make it into a
release.  We've found that using time as the third dimension, as in
our
"data movies," or using several 2D graphs, as in our "multi-graph
overlay" to be more practical solutions.
If you want smoothing or density coloring, that requires binning the
data. Even working at low resolution, you're looking at 256 times as
much time and memory to take the plot into an additional dimension.
You might get a tenfold performance increase with the G5 (which I
think
is quite optimistic, because the G5 adds fast floating point
processing, but binning is a integer operation), but even with that
that, adjusting a gate goes from taking perhaps a second to almost a
half minute.  That would make using FlowJo feel like using CellQuest
(just kidding ;)   At the full resolution of DiVa files, you're
looking
at another thousand fold increase over the 2D version, or a billion
times (1000 ^ 3)  as long as we take to do it now.   So, as best I
can
figure it, we can only support 3D at the cost of losing interactivity
with the data (ie, we can make the views, but changing gates or
parameters won't immediately change the 3D visualization).
It would be tough to have contours in 3D as each layer would obscure
the ones inside it.  Contours would have to have varying opacity,
which
not only increases the computational time and complexity, but would
make it hard to differentiate populations.  And the user interface
for
gating in space would be a real challenge.  You could chop thru space
with planes, but that's 1D gating, which doesn't give you more
capability to define populations than you have now.  So we'd have to
invent polyhedral gating.
If all you want to do is look at already-gated populations in 3D,
there
are options that exist.  Expo32 has this feature, if you can figure
out
how to use Beckman Coulter (actually, ACS wrote it) software to view
BD
files.  You can use existing commercial programs (Aabel & JMP are
nice
Mac 3D applications, and I'm sure there are others), or write it
yourself in OpenGL.  I think OpenGL would be a better choice than C+
+.
OpenGL is a higher level graphics language, and knows how to access
the
specialized accelerators in the graphics cards, which are actually
faster for this stuff than the G5.  That's how the dungeon games do
the
3D shading and rendering.
I'll be happy to donate a considerable amount of code that I've done
in
this effort (most was taken from an old freeware program called
Rotator, which I no longer could find with Google, but I have
somewhere
in my archives), but we decided this was pretty much a dead end.
Rotator was in C, and quite unreadable. For the investment this task
would take, I think it'd be better to start over in OpenGL.  It's
cross-platform too, which is important, as you'll find you want to
port
it to a Cray.
I'd still think you want to use FlowJo to read the DiVa files,
compensate them, transform them, gate them, and then export desired
subpopulations to the 3D viewer.   If it were any other instrument,
you
could probably read the files yourself, with R or our free Java
libraries, but the DiVa files  are a unique format, and almost always
require compensation and transformation to a lin/log scale, so
there's
a lot of work before you even get to viewing them.
I've promised you 3D graphs in FlowJo in the past, and I've done my
best to deliver them, but the results have been pretty
disappointing.
And the benefit of them has never been demonstrated.  If you can show
us how 3D views provide more interpretable data than our current
"compromise solutions,"  that would help.   If you want to pick a
data
file, we'll make you get a spinning, stereoscopic, 3D view of it.  If
we find that other scientists are able to make conclusions about the
data better than they can from our existing visualizations, that will
go a long way towards bumping it up on the FlowJo future feature
list.
I hope that helps.
Adam
On Dec 31, 2003, at 8:38 AM, David Dombkowski wrote:
- Hide quoted text -
- Show quoted text -

- Hide quoted text -

Received on Mon Jan 5 13:58:00 2004
This message: [ Message body ]
Next message: facs_c...@wehi.EDU.AU: "Re: 3D Graphics for flow data
display"
Previous message: Joost Schuitemaker: "intracellular staining of ...
2004"
Maybe in reply to: David Novo: "Re: 3D Graphics for flow data
display"
Next in thread: facs_c...@wehi.EDU.AU: "Re: 3D Graphics for flow data
display"
Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
Subject ]
[ By Author ] [ By messages with attachments ]
This archive was generated by hypermail 2.1.8 : Thu Jan 22 2004 -
12:12:46 EST
VERITY SOFTWARE HOUSE INC. MARKITING AND SALES ON LIST
**************************************************************************************
Re: DNA analysis software
   * This message: [ Message body ] [ More options ]
   * Related messages: [ Next message ] [ Previous message ] [ In
reply to ]
From: Xiaoping Wu <xiaopi...@psbc.org>
Date: Thu May 24 2007 - 17:04:13 EDT
We have Modfit 3.0 and our computer is installed with both OS 9 and
OS
X. We did not find problem with printing.
On May 23, 2007, at 2:33 PM, Turbov, Jane wrote:

- Hide quoted text -

Xiaoping Wu, Ph.D.
Flow Cytometry Laboratory
Research Division of the Puget Sound Blood Center
1201 Ninth Avenue
Seattle, Washington 98101
*****************************************************************
This e-mail and any attachments may contain confidential and
privileged information. It has been scanned for viruses.  If you
are not the intended recipient, please notify the sender
immediately by return e-mail, delete this e-mail and destroy any
copies. Any dissemination, use, review, disclosure, or
distribution of this information by a person other than the
intended recipient is unauthorized and may be illegal.
*****************************************************************
Received on Fri May 25 13:38:00 2007
   * This message: [ Message body ]
   * Next message: Darzynkiewicz, Zbigniew: "RE: Chromatin Structure
Aassay"
   * Previous message: Derek Davies: "Re: Fat-binding colours??"
   * In reply to: Turbov, Jane: "RE: DNA analysis software"
   * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
Subject ] [ By Author ] [ By messages with attachments ]
This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 -
03:12:00 EST
VERITY SOFTWARE HOUSE COMPARES SOFTWARE NOT RELEASED WITH BD SOFTWARE
**************************************************************************************
Purdue Cytometry Mailing List: Analysis software for the Mac
... Purdue Cytometry Mailing List: Analysis software for the Mac ...
programs divide into two price categories, the expensive (CellQuest,
MacLAS, FCAP List, and Verity's WinList for the Mac, not yet
released)
and the relatively ...
http://www.cyto.purdue.edu/hmarchiv/1997/2083.htm - 5.8KB  57%

08 Sep 97
Find Similar
Highlight

EVERY SOFTWARE DEVELOPER AND CORPORATE OWNER ON THE MAIL LIST ABOVE
HAS LICENSE AGREEMENTS ON SOFTWARE…ALL SOFTWARE MUST BE LICENSED….
I DO NOT KNOW IF PURDUE GETS ROYALTIES FROM ALL THE PROVIDERS ABOVE
BUT THE MAIL LIST WAS UNDER TIGHT PROTECTION BY PAUL J. ROBINSON ISAC
PRESIDENT HEAD OF PURDUE CYTOMETRY
************************************************************************************

KANECKI ASSOCIATES INC. INTRODUCED A NEW CYTOMETRY SOFTWARE WITH NO
LICENSE FEE AT A LOW COST TO HELP STUDENTS AND PROGRESS… THE SOFTWARE
IS THE ONLY SOFTWARE THAT DOES NOT USE COMPENSTAION AND CAN PROCESS 10
MILLION EVENT FILES BY 40 PARAMETERS.
*************NO LICENSE FEES AND ONLY $150.00 FOR STUDNETS!
*************

HERE IS HOW THE DISCOVERY OF THE MAIL LIST CAME ABOUT…

FLOW CYTOMETRY SOFTWARE DEVELOPER KANECKI ASSOCIATES INC. WAS CALLED
A
SCAM BY PERDUE J PAUL ROBINSON...THE STORY AND COVER UP THAT IS
WITHIN
THE SYSTEM..WILL THE TRUTH COME OUT?
KANECKI ASSOCIATES INC.INTRODUCES NEW FCS CYTOPRO QUICK FACS 11 TO
PERDUE UNIVERSITY ONLY TO BE INSULTED WITHOUT REASON... UNCOVER THE
SCANDLE THAT IS WITHIN THE SYSTEM AND SEE IF THERE IS A CHANCE FOR
REFORM? WILL IT EVER CHANGE? IT MUST COME TO A END
Nov 22, 2007 16:38
Flow Cytometry Software Developer Kanecki Associates Inc. Gets Called
A SCAM by PERDUE's J PAUL ROBINSON
Posted by cytometryuser
Kanecki Associates Inc. Was SHOCKED when J.Paul Robinson from PERDUE
called them SCAMMERS after being Questioned from the University about
the Response about his prior comment of them sending JUNK MAIL to his
Mail List...THE MAIL LIST seemed to be a very sensitive spot for Mr.
Robinson....
After a email was sent by a undisclosed source with a link providing
a
link to the MAIL LIST everything was uncovered FAST....The link was a
open door to a list of MAIL FROM a group of HIGH POWER
INDIVIDUALS...MOST...SOFTWARE PROGRAMMERS THAT DEVELOPED SOFTWARE
WHILE GETTING EDUCATED THROUGH PURDUE...
KANECKI ASSOCIATES INC. WOULD LIKE TO STATE THEY ARE NOT SCAMMERS NOR
DID THEY EVER ATTEMPT TO GET ON THE PERDUE MAIL LIST...They have
never
sent mail through the list but just tried to introduce the NEW
software that is distributed through C/net, Best Buy, Circuit City,
Simtel, and may other Major Down load sites.
To view the software JUST       GOOGLE:  FCS CYTOPRO QUICK FACS 11.
KANECKI ASSOCIATES INC. RELEASED THE NEW FCS CYTOPRO JAVA 6.0 FOR
DOWNLOAD TOO.
**********************WITH NO LICENSE
FEES******************************** AND REASONABLE PRICES THEY
DELIVER A SUPERIOR PRODUCT THAT PROCESS 10 MILLION EVENT FILES...FCS
3.0
THEY DO NOT USE COMPENSATION LIKE OTHER SOFTWARE PROVIDERS SO THERE
IS
NO OVER COMPENSATION ISSUES........Further More there is NO MENUE BAR
which makes for USER FRIENDLY SOFTWARE...VERY EASY TO USE....JUST
PICK
AND CLICK.................................
SO TO PROVIDE THE TRUTH ALL POST THAT WILL BE SHOWN CAME DIRECTLY
FROM
SAVED EMAIL SENT TO PURDUE...IT WAS NOT SENT TO THE LIST BUT TO THE
HEAD OF  PURDUE..
NOT MR.ROBINSON HE RECEIVED A CC...THEN RESPONDED BU CALLING IT JUNK
MAIL!
ORIGINAL MESSAGE SENT      :    TO ROBERT MURPHY  WITH LINKS FOR
DOWNLOAD AND ALL INFORMATION PERTAINING TO FCS CYTORPO QUICK FACS 11
SOME HOW IT WAS FOWARDED TO: J PAUL ROBINSON...MAYBE FOR REVIEW...?
Fw: NEW FLOWCYTOMETRY SOFTWARE FOR ALL BD and Beckman/Coulter
Flow Cytometers CAN PROCESS 24,000 SAMPLES PER HOUR
Standard
Header|Full Message
View
mitchell haynes <buybro...@yahoo.com>
ViewFriday,
September 28, 2007 1:36:36 AM
To:j...@flowcyt.cyto.purdue.edu
Cc:da...@kanecki2.com
Cytopro
quick facs savings analysis.doc (82KB); Setup-Quick
Facs 11 Demo.exe (7854KB); FCS
CYTOPRO BENIFITS.doc (25KB)
Find:
Dear Robert Murphy
NEW
FLOWCYTOMETRY SOFTWARE FOR ALL BD and Beckman/Coulter Flow Cytometers
CAN
PROCESS 24,000-42,000 SAMPLES PER HOUR.
FLOW CYTOMETRY    FCS CYTO PRO QUICK FACS
http://www.kanecki.com/flow.html
Increased quality and productivity. With 10,000,000 event files, you
can process 24,000 samples/hour, and maintain quality up to Sigma 5
or
better. Compare this to having your research technologist performing
only 100 samples/hour analysis.
Increased laboratory utilization by 3X because you can perform the
analysis off-lab and free laboratory time for reading samples. This
was achieved when I developed the program, and we had a program
project grant from 1992 to 1998 of $8M.
Works with FCS 3.0 in all data modes as floating point, integer*4,
and
ASCII.
Works with BD and Beckman/Coulter Flow Cytometers and Cell Sorters
Backwards compatible with FCS 2.0 files and Flow Cytometers and Cell
Sorters.
Can read FCS 3.0 files up to 10M events with 20 parameters.
Easy to Use, three step process. Load initial file, set gate, specify
file list to process. That's it.
Collaboration tools to allow you to cut and paste image results to
results.
Statistical analysis results imprinted on histogram plots directly as
mean, mode, and median with the ability to present results in log
mode
or linear mode, depending on the detector used.
Plain vanilla coding for easy update and maintenance to allow for the
greatest user and software quality.
One time purchase fee, no yearly renewal fees as with others.
Proven tract record in FACS, Fluorescent Activated Cell Sorter
Laboratory. The laboratory was rated the best laboratory in the
Midwest USA in 1990.
This application is designed for large-scale fluorescent activated
cell sorter
analysis. The program can read up to FCS 3.0 files and has been
tested
to run on Becton Dickinson and CoulterOrtho based flow cytometers and
cell sorters. The main advantage of this program is that you can have
the computer perform the analysis for you after you have selected the
region to analyze. The result is that up to 24,000 samples per hour
can be analyzed on a 1.4 GHz speed computer.
This program is designed for researcher and technologist use. It uses
rectangular gating, and is intuitive to use. To use this program, the
FCS must have the extension, *.bin as "54203023.bin" as an example.
The *.bin extension is what the computer uses to locate the files on
the computer.
THANK YOU FOR YOU TIME
MITCHELL HAYNES
VP
SALES
KANECKI
ASSOCIATES
832-347-1669
THIS WAS J PAUL ROBINSON RESPONSE TO THE INFORMATION SENT
Re: Fw: NEW FLOWCYTOMETRY SOFTWARE FOR ALL BD and Beckman/Coulter
Flow
Cytometers CAN PROCESS 24,000 SAMPLES PER HOUR...Standard Header|Full
Message View J. Paul Robinson J. Paul Robinson ... ViewFriday,
September 28, 2007 9:37:32 AM To:mitchell haynes
Cc:da...@kanecki2.com; skel...@flowcyt.cyto.purdue.edu
Steve
what is this email - it came to me with Bob Murphy's name associated
with it. It seems to be an advertisement, this junk mail, and it
seems
to have been modified by you ...
So I guess I am confused. was this sent to the list, or do you have
an
details about it - i am concerned about these junk messages going out
to
our members, - if they are using our lists, I will deal with them
appropriately, but I am not happy about this - any info you can give
me
appreciated
thanks
paul
WHILE BEING SHOCKED BY THIS MAIL...MITCHELL HAYNES VP.KANECKI
ASSOCIATES INC....WROTE BACK

- Hide quoted text -

<!--[if !supportLineBreakNewLine]-->
AFTER THAT MESSAGE J PAUL ROBINSON RESPONDED WITH OUT REASON!

- Hide quoted text -

----- Original Message ----
From: "j...@flowcyt.cyto.purdue.edu"
<j...@flowcyt.cyto.purdue.edu>
To: mitchell haynes <buybro...@yahoo.com>
Cc: j...@flowcyt.cyto.purdue.edu
Sent: Friday, September 28, 2007 2:26:51 PM
Subject: Re: Fw: NOT A JUNCK MAIL STEVE WAS JUST FOWARDING
INFORMATION
TO
PROPER CHANNELS
Sorry, I think it is junk mail
regards
paul robinson
After this message Kanecki Associates Inc. JOINED many Forums in
which
the MESSAGES WITH THE NAME OF THE SOFTWARE AS RELAYED.....JUST ABOUT
ALL FORUMS DELETED INFORMATION POSTED ABOUT THE
SOFTWARE...FURTHERMOORE...BEING REMOVED AND BANNED FROM FORUMS....
VERY CONFUSED ABOUT ALL THE TALK OF OTHER SOFTWARE THE LAUNCHED
QUESTIONS ON YAHOO..?
WHY DO FORUMS ALLOW THE CONVERSATION OF ONE SOFTWARE AND NOT
ANOTHER..
WHY DO FLOW CYTOMETRY COMPANIES CHARGE LICENSE FEES?
THEN SOMEONE SENT A LINK TO THE PURDUE MAIL LIST BUT IT WAS NOT TO
THE
FRONT BUT BACK ROUTE..WHEN  VIEWED ALL THE REASONS WERE EASY TO
SEE...........
J PAUL ROBINSON HAD A LOT OF POWER AND INFLUENCE IN THE FLOW
COMMUNITY...
FURTHER MOORE EVERY PROGRAMMER THAT HAS SOFTWARE ON THE MARKET WENT
TROUGH THE MAIL LIST SINCE 1992 AND ALL HAD THEIR OWN SOFTWARE FOR
SALE ON THE PERDUE SITE.....THE POST IN THE MAIL LIST ARE UNFILTERED
AND WERE******* NOT WHAT THE REST OF THE LIST HAD ACCESS TO...ONCE
POST STARTED GOING UP THE SITE WAS BLOCKED... SINCE THEN BOTH SITES
HAVE BEEN UP AND DOWN FOR THE MAIL LIST!
WITH THE MARKET BEING VERY SMALL IN FLOW SOFTWARE IT IS EASY TO
CONTROL....
KANECKI ASSOCIATES POSTED A BLOG  WITH THE LINK TO THE BACK ROUTE TO
THE MAIL LIST AND GOT IMMEDIATE RESPONCE...
WHEN MITCHELL HAYNES DISCOVERED THE LINK HE STARTED POSTING THE
DISCOVERY UP ON MIS.HEALTH AIDS THROUGH :::GOOGLE::: AS FOR*****
GOOGLE*****IS THE ONLY SITE NOT FILTERING THE MAIL....
FINALLY A THE MESSAGES WERE SEEN AND SINCE THEY CAME FROM THE MAIL
LIST POSTINGS  THEY WERE NOT PRETTY....
ALL FILES WERE DOWNLOADED
WHAT CAN KANECKI ASSOCIATES INC FCS CYTOPRO JAVA  6.0 DO? NO LICENSE
FEE
0NLY $150.00 FOR STUDENTS 250.00 USD EVERYONE ELSE
*********************************************************************************************************************
POSTED BY MITCHELL HAYNES
IT IS NOT A SCAM OR JUNK MAIL PLEASE READ!
Written by David Kanecki, MBA, ACS, Bio. Sci.
November 25, 2007
Fcs cytopro quick Facs 6.0 java  14
       The FCS Cytopro Quick Facs 6.0 Java allows the performance of
flow
cytometry analysis on computers or PC. This new technology allows for
utilization on Mac, Linux,
Solaris, and Windows with the same functionality and processing
speed.
It is designed to minimize the learning curve by doing away with a
confusing menu bar.
This new easy navigation process allows for any level of student,
technologist or researcher to perform flow cytometry analysis with
ease. The utilization time can be used   inside or outside of the
lab.
Easy operation allows for greater efficiency and quality. This
translates into decrease in time and cost.
About  27% overall savings in flow cytometry experiments. So,Just
pick, click and go!
This program can read three of the four most common modes of the FCS
3.O  files, and is
Backward compatible with FCS 2.O and 1.O files
Equipped with advanced processing speeds allows rapid results, no
need to wait for your computer to think. When performing a batch mode
analysis, you can analyze about 24 to 42,000 samples per hour with
FCS
2.0 files, and about 4,000 samples per hour with 1 million event by 9
parameters for FCS 3.O files. Also 10 million event by 25 parameter
FCS 3.0 file or a 10 million event  FCS 2.0 and 1.0 files.
This FCS Cytopro Quick Facs Java 6.O is for large-scale and rare-
event analysis.
There is no Dongle, License fees, or restrictions for using the
software. The user can process the analysis where ever and whenever
necessary.
There are various features this program has to offer consisting of
analyzing dot plots, dual histograms , and descriptive statistics.
Easy to use just copy and paste the items into your report or paper.
This shows the result of a du
al, histogram analysis along with dual, descriptive statistical
analysis. The first histogram is in red, and the second is in blue.
The first, descriptive
analysis gives the information for the red histogram, and the second
descriptive analysis,
below the first, gives the information for the blue histogram. Also,
in the result box, see the two files listed along with their
checksums. The checksum is a measure that allows you to monitor the
correctness of a file. If a file is the same as the last time, its'
checksum will be the same. If the file is different, same size, but
different data, then its' checksum will be different:
Conclusion
Without a dongle, license fees and excessive time wasted educating
to
on software usage,  Kanecki Associates Inc will save the world
millions.
There will be no need for large seminars and wasted time on how to
utilize software and more time to study important issues. Students
can
learn without fear of being able to afford the software. For only one
hundred and fifty dollars USD. for students. Yes you can own it.
There
is no license fees.
If you are not a student the cost is only two hundred and fifty
dollars USD .So why lease your software from the lab at thirty
dollars
per hour?  Within five hours of leasing software from you lab at 30
dollars per hour you could be at home relaxing and working on you PC.
If you are not a student the cost is only two hundred and fifty
dollars.  Never a license fee or dongle. You can take it or leave it,
where ever  you like!
       The FCS Cytopro Java 6.0 program allows you to analyze large
sample
sets
quickly, easily, and with high-quality and precision.  In addition,
it
can analyze large data
set files of up to 10 million events and 25 parameters for use in
rare-
event analysis or
kinetic  based experiments. This program is designed so that it is
easy to use, and this ease
of use and accuracy translates into high productivity and cost
savings, about 27% overall
in flow cytometry cost.
New technology breaking down the walls developed by Dr. David
Kanecki,
No it is not a Scam or Junk mail! It is  high tech software at a
reasonable price.
Free trial down load so there is nothing to loose
http://www.kanecki.com
http://www.flowcytometrysoftware.com
direct download link for free trial
http://www.flowcytometrysoftware.com/order.html
FCS Cytopro Java 6.0 Script (c) 2007 By D. Kanecki, All Rights
Reserved
Mi...@Kanecki.com
WHAT PEOPLE SAY ON THE MAIL LIST ABOUT THE PRICE AND LICENSE FEES FOR
SOFTWARE!
FACS-software
   * This message: [ Message body ] [ More options ]
   * Related messages: [ Next message ] [ Previous message ] [ Next
in thread ] [ Replies ]
From: Yuecel, Raif <Raif.Yue...@pmintl.com>
Date: Wed Nov 02 2005 - 06:32:42 EST
Dear FACS-experts,
I need the opinion of the specialist:
I need FACS-software for PC and had decided for FlowJO. I know the
mac-version of FlowJO. Indeed, this is very expensive.
Now, I was asked to get a comparative offer. Therefore, I thought
about
WinList. This is much cheaper. But I have no experience with WinList.
My question to the experts:
1.      What are the advantages and disadvantages of both software?
Question of the business & finance department, which are also
entitled:
1.      Why is FlowJo so expensive, if you need several licence?
2.      Why FlowJo and not WinList, because WinList much cheaper?
Thanks very much for the help !
Best regards
Raif
-----------------------------
Dr. Raif Yuecel
PHILIP MORRIS Research Laboratories GmbH
Fuggerstrasse 3
D-51149 Koeln
Germany
Phone    +49 2203 303 486
Fax         +49 2203 303 362
=====================
This e-mail message is for the sole use of the intended recipient(s)
and may
contain confidential information. Any unauthorized review, use,
disclosure
or distribution is prohibited. If you are not the intended recipient,
please
contact the sender by reply e-mail and destroy all copies of the
original
message.
Diese e-mail ist ausschließlich für den/die Adressaten bestimmt und
kann
vertrauliche Informationen enthalten. Jede nicht genehmigte Nutzung,
Veröffentlichung oder Weitergabe ist untersagt. Sollten Sie nicht der
für
diese e-mail bestimmte Adressat sein, kontaktieren Sie bitte den
Absender
und vernichten alle Kopien der Original-Nachricht.
Received on Wed Nov 2 17:38:00 2005
   * This message: [ Message body ]
   * Next message: Robert C. Leif: "RE: FCS files and software
compatability"
   * Previous message: Stingley, Robin L: "FACSAria service"
   * Next in thread: David Novo: "Re: FACS-software"
   * Reply: David Novo: "Re: FACS-software"
   * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
Subject ] [ By Author ] [ By messages with attachments ]
This archive was generated by hypermail 2.1.8 : Sat Jan 14 2006 -
22:03:58 EST
***************************************************************************-
***********************************************
Prdue University Cytometry Laboratories - Flow cytometry
confocal ...... please go to the rippoff website above and make your
complaint and register. ... J. Paul Robinson, Ph.D., Director PUCL,
1203 W. State St., Purdue ...
www.cyto.purdue.edu/flowcyt/rebates.htm?institutionId=694&meetingId=218
******************************************************************************************************************************
FlowJo hiring, come see us at ISAC
This message: [ Message body ] [ More options ]
Related messages: [ Next message ] [ Previous message ]
From: Adam Treister <a...@treestar.com>
Date: Sun May 14 2006 - 20:04:57 EDT
Come join the FlowJo team!
***************************************************************************¬
********************************************
If you're not coming to ISAC, you must not be cool, and we don't
want
to hire you.
***************************************************************************¬
*************************************************
If you're not coming to ISAC, you must not be cool, and we don't
want
to hire you.
***************************************************************************¬
************************************************
If you're not coming to ISAC, you must not be cool, and we don't
want
to hire you.
***************************************************************************¬
**********************************************
If you're not coming to ISAC, you must not be cool, and we don't
want
to hire you.
***************************************************************************¬
************************************************
We are looking to expand FlowJo's market presence, train a
generation
of scientists in the subtle beauty of high color flow, and solve
some
small subset of the world's problems.  If you're coming to ISAC
XXIII
in Quebec, and are interested in making the cytometry world a better
place, please bring by your CV and talk to us in Booth 301.  If you
don't need a job, at least come by to get a tie-dyed t-shirt.
If you're not coming to ISAC, you must not be cool, and we don't
want
to hire you. Unless, of course, you are an exceptional flow user,
with good grasp of the theory behind the machine, know at least ten
ways to hold down modifier keys to perform silly FlowJo tricks, and
want to share your expertise with a growing user community.  Then,
please send your CV to j...@treestar.com, and we'll evaluate your
feeble excuse for missing this important conference.  We'll send you
a t-shirt if we have any left.
Adam
-------------------------------------
Adam Treister
President
Tree Star, Inc.
340 A Street
Ashland OR 97520
www.flowjo.com
-------------------------------------
Received on Mon May 15 14:18:00 2006
This message: [ Message body ]
Next message: PLo...@adarc.org: "MetroFlow Meeting"
Previous message: Chuanwu Xi: "RE: Emailing: MicroFlow_List"
Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
Subject ]
[ By Author ] [ By messages with attachments ]
This archive was generated by hypermail 2.1.8 : Tue May 16 2006 -
04:12:02 EDT

*******************************************************************************************************************************
2007 End of year message from Purdue
•    This message: [ Message body ] [ More options ]
•    Related messages: [ Next message ] [ Previous message ] [ Next in
thread ] [ Replies ]
From: J. Paul Robinson <jpr@flowcyt.cyto.purdue.edu>
Date: Fri Dec 28 2007 - 13:43:46 EST
Beware, the end is nigh!
No, not an apocalyptic prediction - but 2007 is definitely coming to
an
end. Not before time I would say - it s been a busy year. But I have
some strong words to end the year and I am going to say them!! Of
course
you don t have to read them!

Cytometry is now 40 years old and it s been sort of decaying a bit.
What
do I mean? I am amazed at how conservative and frankly boring the
field
has become. Why? It s time to move to the 21st century folks. I'm
getting older and frankly, its time to kick some butt as my younger
colleagues often say. We talk so much like it is the same old
cytometry
it has always been. Wake up people - times are changing - look at all
these new small companies trying to stick their noses in "our" field!
True we need to do the core work and do it well, but lets not forget
that fundamental tools of cell analysis are changing and if we don't
keep ourselves up-to-date and educated on what's happening....before
we
know it, a new field will emerge and we will be like the old electron
microscopists who are still wondering what happened ......

I know most of us work in the field and like what we do, but I think
its
time to open up a little and try to do some serious integration of
our
field. It s not happening very effectively on the most part I would
say.
Cytometry is about integration of the tools of the field into the
vast
reaches of biological problems that we can contribute to solving.
Cytometry is about advancement of the field, that means always
looking
ahead. ISAC will soon be the International Society for Advancement of
Cytometry   a 21st century Society not a 20th century Society.

Cytometry is not flow cytometry!! Let s not kid ourselves about this
folks. Cytometry is about measuring cells - however you do it - and
flow
cytometry is just one component of many. I understand that it may be
the
only tool some of you use - I don t want to take away from that or
de-emphasize its value or importance. But, we so often hear people
talk
about our field only in context of just flow cytometry. Recently,
when
we polled the ISAC community on changing our name from "analytical
Cytology" to Advancement of Cytometry" we received comments like "hey
I
don t do flow cytometry, so why are you reducing the breadth of the
field?" Ouch - they think "cytometry" means "flow cytometry". We have
a
long way to go before we convince the community that we cover all
aspects of cytometry. And let s also remember the growing membership
in
India and China   (that s half the worlds population right there)   it
s
high time we paid much more attention to these countries as a field.
Awtar Krishan can t be the only person to drive cytometry training
and
education for 1.2 billion Indians can he? Well he has been up to now.
Who is taking on the mantle of training and education of cytometry in
China?

So here's the scoop. That's one of the reasons why the Purdue Web
portal
is going to change. We tried to make the change this past year, but
there were too many other things happening here to achieve it. But
come
middle of 2008, I am resolved that you will see a huge difference in
the
Purdue site. It s been the default cytometry communication portal now
for many, many years. We have focused on good clean fun with cytometry
-
quality, timely, simple - no spam. Many people like that actually.
The
portal is almost overwhelming for us   22,000 daily page requests
with
over 2 Gigs daily download. In 2007 alone, downloads of 208,000
powerpoint files, 233,000 PDF files, 8800 movies, 38,000 word
document
files. The education pages and the Cytometry Discussion Archive are
the
most hit for sure. Over 125,000 distinct files from our portal were
accessed in 2007.

But all good things must come to an end. Come July 2008, the usual
Purdue web portal may well be no more. It will be replaced with
something entirely new. Hopefully most will find it more useful and
relevant - some will not like it. Maybe we will be able to make
everyone
happy....ha!..C'est la vie. Some of you will be beta testers and
advisers I hope.

So my best wishes to all in the cytometry field for 2008. Regarding
the
past year on the discussion list, its been lively, with an average of
7
messages per day with 754 different individuals submitting at least
one
message. 139 messages had at least 6 responses.  There were 1205
unique
subject lines. Subscribers came from 64 top level domains. The usual
bunch of suspects answered lots of messages and Marty Bigos seems to
have too much time as he answered the most (thanks Marty!!).
Tragically,
the second most prolific responder was Randy Fisher who passed away
on
December 5.  Randy's responses were always short, to the point and
accurate. It hurts to lose one of our own, particularly when it's one
of
our most active members. But that s the point isn't it. For many
years
to come, we have the value of Randy's hundreds of suggestions over
the
years archived for the many new people who enter our field. Many of
you
probably never actually met Randy - but I bet most of you feel you
knew
him. One of the mysteries of the web I suppose. Our condolences to
Randy's family - perhaps they didn't know how many people knew Randy
"electronically" - but we all did. You know we are a small field when
it
comes to the big world of science so when we lose one person, the
entire
field morns.

To end 2007, let me make a big plug for a program we began at the
2006
ISAC congress. Gary Durack from iCyt and myself started a small
not-for-profit charity called "Cytometry for Life" in response to
Stephen Lewis' compelling plea for some low cost CD4 devices. Our
field
has done a lot of talking about this, but only a few people have
really
tried to do anything practical. Well, folks we have all been doing
cytometry for a very long time - it's time to do something.
"Cytometry
for life" (http://www.cytometryforlife.org) is working hard. We have
made tremendous progress in just one year. It would be great if you
all
decided to jump on board and play a small part. You can give money,
advice, moral support, talk to your politicians, community health-
care,
charities, whatever. But get involved as be recognized as the
cytometry
community to solve this problem of bringing low cost, portable
devices
to the 65% or more of African's who don t live in the cities and
towns
where current CD4 technologies are available. Our goal is to work in
areas not being served by current technologies. We have heard these
calls before, but folks we have to deal with this problem - it's your
problem if you call yourself a "cytometry" person. Email me if you
can
help - consider donating to the program, let's make it work. By the
end
of 2008, I want to be telling you that the program is getting to
people
who need this desperately. Help us achieve that for 2008.

I hope many of you got hold of a copy of our new double DVD set
Cytometry   60 years of Innovation    if not ask your local rep from
virtually any company in our field. It might give you a good sense of
how strong the foundation in our field really is. I will see many of
you
at the 2008 congress in Budapest. I know some of you think its going
to
be expensive so I took several hours myself and created a webpage for
the cheap ones out there so you have no excuses not to go...
(http://www.cyto.purdue.edu/flowcyt/cheapflights1.htm).

It's been a privilege to serve for the past 19 months as President of
ISAC. I will gladly pass that hat to Bob Murphy in May. ISAC is alive
and well  - membership is growing daily. I would not be surprised to
see
us top 2000 by the end of the Congress in May. I know that about 60%
of
the members of this list are NOT ISAC members. Perhaps you should
consider joining the Society that keeps many of you in business?
http://www.isac-net.org/

My best wishes for you all in 2008 from Purdue
Paul

I have
some strong words to end the year and I am going to say them!! Of
course
you don t have to read them!

I have
some strong words to end the year and I am going to say them!! Of
course
you don t have to read them!

We talk so much like it is the same old cytometry
it has always been. Wake up people - times are changing - look at all
these new small companies trying to stick their noses in "our" field!

We talk so much like it is the same old cytometry
it has always been. Wake up people - times are changing - look at all
these new small companies trying to stick their noses in "our" field!

--
J. Paul Robinson
SVM Professor of Cytomics
Professor of Immunopharmacology & Biomedical Engineering
Director, Purdue University Cytometry Laboratories
President, International Society for Analytical Cytology

Purdue University Cytometry Laboratories
Bindley Bioscience Center
1203 West State Street
Discovery Park, Purdue University
West Lafayette, IN 47907-2057
Ph (765) 494 0757; Fax (765) 494 0517
email: jpr@flowcyt.cyto.purdue.edu
www.cyto.purdue.edu

Join ISAC - www.isac-net.org

Change lives today  - www.cytometryforlife.org
Received on Fri Dec 28 15:18:00 2007
•    This message: [ Message body ]
•    Next message: rob.sutherland@utoronto.ca: "Re: [ TdT Antibody
Control? ]"
•    Previous message: slsacia@gundluth.org: "[ TdT Antibody Control? ]"
•    Next in thread: Darzynkiewicz, Zbigniew: "RE: 2007 End of year
message from Purdue"
•    Reply: Darzynkiewicz, Zbigniew: "RE: 2007 End of year message from
Purdue"
•    Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
Subject ] [ By Author ] [ By messages with attachments ]
This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 -
03:12:00 EST
*************************************************************************************************************************
Re: mr on Apple web site
•    This message: [ Message body ] [ More options ]
•    Related messages: [ Next message ] [ Previous message ] [ In reply
to ] [ Next in thread ] [ Replies ]
From: Howard Shapiro <hms@shapirolab.com>
Date: Wed Aug 25 2004 - 18:15:38 EST
Robin Barclay wrote:

I can't pass up the opportunity to get into the PC/Mac battle - I hate
them
both, but there aren't really alternatives (yes, I know, there's
Linux, but
it's probably even harder to hook up a Linux system to hardware than
it is
to hook a Mac up to hardware).

Of course, what started this thread was the assertion in the piece
about
Mario on Apple's web site that there were 15,000 to 20,000 Mac-based
flow
cytometers out there. The best estimates I had from industry people
in
early 2003 when I was finishing up the 4th Edition of PFC was that
the
total number of systems from all manufacturers in use was under
20,000. If
that's correct, BD would need a 75% market share to account for the
low end
estimate of 15,000 Mac-based machines. I'd be interested to know where
the
15,000 to 20,000 figure came from.

The old (68000 series and possibly early PowerPC) Macs were difficult,
but
not impossible to connect to hardware; it was easier to work with the
PC's
ISA bus, which, while slow, was perfectly adequate to do most flow
cytometric data acquisition and analysis. There were decent versions
of
Forth, which was one of the first and best languages designed for
controlling hardware from mini- and microcomputers, for both PC (DOS)
and
the Mac (Forth was the first Mac programming language made
commercially
available, at a time when the only other option was buying the very
expensive Lisa from Apple on which to develop Mac software). I found
MacForth easier to program with than the Windows Forths (or other
Windows
languages, e.g., Delphi), but my old Macs used to crash all the time.

So what makes me unhappy with both Windows [and Windows machines] and
the
Mac in their current incarnations? It is now significantly harder for
mere
mortals to write software to get hardware to communicate with either
PCs or
Macs; the gain in complexity associated with the PCI bus, USB/USB2,
FireWire(IEEE 1394), etc. is greater than the gain in speed and
convenience. Also, in making the operating systems more stable (and
Win XP,
despite its security issues, is almost as stable as Mac's Unix-based
OS X),
both Microsoft and Apple elected to eliminate the ability of their
computer
hardware to respond rapidly to interrupts (latencies are now in the
tens of
thousands of instruction cycles), meaning that any really fast
hardware
attachment for either a PC or a Mac now needs to have a DSP in it,
whereas
if the fast interrupt response had been preserved, the hardware
attachments
could have been made much simpler and cheaper. Linux also takes fast
interrupt response off the table, so it doesn't represent a viable
alternative. If you go to Apple's web site and look at what data
acquisition hardware is available for Macs, particularly for Macs
running
OS X, there isn't much, and many of the companies that supported the
Mac in
that area have dropped Mac support for their newer products. That is
undoubtedly one big reason why BD's newer digital pulse processing
cytometers are running on the PC platform.

That doesn't stop anybody from analyzing FCS files on Macs. FlowJo is
well-conceived flow cytometry software; one reason it is as good as it
is
is that it was written by people who do a lot of flow cytometry, and
cutting edge flow cytometry at that. But there are other people who do
a
lot of good flow cytometry who have written good software, for PCs as
well
as for Macs.

For the record, I have a G4 PowerBook, which I use mostly for the
iLife
applications, which are slick. It doesn't crash more than once every
couple
of months, but the same is true of my Windows 2000 and XP systems. OS
X can
be as infuriating as Windows when one or another aspect of it goes
counter
to your intuition or to what you have gotten used to. Macs, while
somewhat
more expensive, are much better made than many PCs, and they are
certainly
aesthetically pleasing. If there were a reasonable alternative to
Microsoft's Office applications for the Mac, I might consider
switching.
The 12.1" PowerBook is a nice portable, but it's over a pound heavier
than
the Fujitsu laptop I now use, which has pretty much the same speed,
memory,
and drive capabilities (OK, not DVD-R, but I don't burn a lot of
DVDs).

However, I really wish Apple had stuck with the plan they had a few
years
back of writing a Mac operating system for Intel hardware. I think
that
died when Microsoft bailed Apple out with a few hundred mil. If it
were
possible to run OS X and XP mano a mano on the same hardware, there'd
be a
more rational basis for comparison. But, as may be the case for the
November election, minds, once made up, are not easily changed.

-Howard
Received on Thu Aug 26 16:38:00 2004
•    This message: [ Message body ]
•    Next message: Konz, Richard: "Discrimination of GO/G1"
•    Previous message: Ann Atzberger: "RE: optimum concentration for
sorting"
•    In reply to: Robin Barclay: "Re: mr on Apple web site"
•    Next in thread: Robert C. Leif: "RE: mr on Apple web site"
•    Reply: Robert C. Leif: "RE: mr on Apple web site"
•    Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
Subject ] [ By Author ] [ By messages with attachments ]
This archive was generated by hypermail 2.1.8 : Sat Aug 28 2004 -
03:12:06 EST
********************************************************************************************************************************
Re: 3D Graphics for flow data display
This message: [ Message body ] [ More options ]
Related messages: [ Next message ] [ Previous message ] [ Maybe in
reply to ] [ Next in thread ]
From: Adam Treister <a...@treestar.com>
Date: Sun Jan 04 2004 - 13:48:24 EST
David,
We've tried on a couple of occasions to add a "spatial" 3D module to
FlowJo, and it has never turned out well enough to make it into a
release.  We've found that using time as the third dimension, as in
our
"data movies," or using several 2D graphs, as in our "multi-graph
overlay" to be more practical solutions.
If you want smoothing or density coloring, that requires binning the
data. Even working at low resolution, you're looking at 256 times as
much time and memory to take the plot into an additional dimension.
You might get a tenfold performance increase with the G5 (which I
think
is quite optimistic, because the G5 adds fast floating point
processing, but binning is a integer operation), but even with that
that, adjusting a gate goes from taking perhaps a second to almost a
half minute.  That would make using FlowJo feel like using CellQuest
(just kidding ;)   At the full resolution of DiVa files, you're
looking
at another thousand fold increase over the 2D version, or a billion
times (1000 ^ 3)  as long as we take to do it now.   So, as best I
can
figure it, we can only support 3D at the cost of losing interactivity
with the data (ie, we can make the views, but changing gates or
parameters won't immediately change the 3D visualization).
It would be tough to have contours in 3D as each layer would obscure
the ones inside it.  Contours would have to have varying opacity,
which
not only increases the computational time and complexity, but would
make it hard to differentiate populations.  And the user interface
for
gating in space would be a real challenge.  You could chop thru space
with planes, but that's 1D gating, which doesn't give you more
capability to define populations than you have now.  So we'd have to
invent polyhedral gating.
If all you want to do is look at already-gated populations in 3D,
there
are options that exist.  Expo32 has this feature, if you can figure
out
how to use Beckman Coulter (actually, ACS wrote it) software to view
BD
files.  You can use existing commercial programs (Aabel & JMP are
nice
Mac 3D applications, and I'm sure there are others), or write it
yourself in OpenGL.  I think OpenGL would be a better choice than C+
+.
OpenGL is a higher level graphics language, and knows how to access
the
specialized accelerators in the graphics cards, which are actually
faster for this stuff than the G5.  That's how the dungeon games do
the
3D shading and rendering.
I'll be happy to donate a considerable amount of code that I've done
in
this effort (most was taken from an old freeware program called
Rotator, which I no longer could find with Google, but I have
somewhere
in my archives), but we decided this was pretty much a dead end.
Rotator was in C, and quite unreadable. For the investment this task
would take, I think it'd be better to start over in OpenGL.  It's
cross-platform too, which is important, as you'll find you want to
port
it to a Cray.
I'd still think you want to use FlowJo to read the DiVa files,
compensate them, transform them, gate them, and then export desired
subpopulations to the 3D viewer.   If it were any other instrument,
you
could probably read the files yourself, with R or our free Java
libraries, but the DiVa files  are a unique format, and almost always
require compensation and transformation to a lin/log scale, so
there's
a lot of work before you even get to viewing them.
I've promised you 3D graphs in FlowJo in the past, and I've done my
best to deliver them, but the results have been pretty
disappointing.
And the benefit of them has never been demonstrated.  If you can show
us how 3D views provide more interpretable data than our current
"compromise solutions,"  that would help.   If you want to pick a
data
file, we'll make you get a spinning, stereoscopic, 3D view of it.  If
we find that other scientists are able to make conclusions about the
data better than they can from our existing visualizations, that will
go a long way towards bumping it up on the FlowJo future feature
list.
I hope that helps.
Adam
On Dec 31, 2003, at 8:38 AM, David Dombkowski wrote:

- Show quoted text -

Received on Mon Jan 5 13:58:00 2004
This message: [ Message body ]
Next message: facs_c...@wehi.EDU.AU: "Re: 3D Graphics for flow data
display"
Previous message: Joost Schuitemaker: "intracellular staining of ...
2004"
Maybe in reply to: David Novo: "Re: 3D Graphics for flow data
display"
Next in thread: facs_c...@wehi.EDU.AU: "Re: 3D Graphics for flow data
display"
Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
Subject ]
[ By Author ] [ By messages with attachments ]
This archive was generated by hypermail 2.1.8 : Thu Jan 22 2004 -
12:12:46 EST