31. Identify which organizations you are a current member of. Use one
line per organization.
32. Are you aware of ISAC? (International Society for Analytical
Cytology)
Yes
No
33. Are you a member of ISAC
Yes
No
34. If ISAC were to establish a strong interest in the area of HCS,
would you find it more attractive to join and work within this
organization?
Yes
No
***********************************ANOTHER FOR MEMEBERS ONLY RUN BY J
PAUL ROBINSON PRESIDENT ISAC 2008

31. Identify which organizations you are a current member of. Use one
line per organization.
32. Are you aware of ISAC? (International Society for Analytical
Cytology)
Yes
No
33. Are you a member of ISAC
Yes
No
34. If ISAC were to establish a strong interest in the area of HCS,
would you find it more attractive to join and work within this
organization?
Yes
No

http://www.cyto.purdue.edu/hcs/

High Content Analysis/Screening

1. What is the goal of this Survey?

This is a long and detailed survey - but we need your help. Please do
it!!! This survey is the result of a meeting of about 250 people who
attended a recent meeting in San Francisco (January 2004) that
focused
on the issues surrounding High Content Screening (HCS) or Analysis.

Several important issues arose from the meeting including the
direction and complexity of systems, and the overall impact of
informatics on the field. Many members felt that if more information
were gathered from participants, some aspects of HCS could be made
more credible and more valuable to users.

This survey is independent of corporate influence in either the
instrument, reagent or pharmaceutical companies.

This is quite a long survey and will require several minutes of your
time. However, without quality data, it will be difficult for us to
evaluate issues and create resources for the HCS community.
1. First, we would like to thank you for working your way through
this
survey. If you assist us by answering the questions with care and
much
thought, we are sure that you will benefit as well as the entire
community. At the end of the survey, you will be offered a chance to
join a discussion forum.

Please tell us your affiliation: (tick only one)
Pharmaceutical company
Assay service company

Reagent manufacturing company
Instrument Manufacturer
Academia
Private Research Institute
Other (please specify)
2. How many employees are there in your organization?
1-10
11-50
51-100
101-500
501-1000
1001-5000
5001-10,000
greater than 10,000
3. Is your HCS infrastructure considered a core facility (available
for use by other groups at your organization)?
Yes
no
Other (please specify)
4. Is your group a part of an "autonomous" research group?
Yes
No
Other (please specify)
5. What is your personal scientific area of interest? (Click all that
are applicable)
Neurobiology
Cancer research
Endocrinology
Immunology
Toxicology
General Biochemistry
Molecular Biology
Microbiology
Biomedical Engineering
Biotechnology
Informatics
Computer sciences
Imaging
Automation and robotics
Other (please add here)
6. What type of biological phenomenon are you interested in? Check
all
that apply.
Signalling Pathways
GPCRs
Differentiation phenotypes
Translocations
Other (please specify)

High Content Analysis/Screening

2. Instrumentation

Can you give us some idea of what instruments are the key tools for
your HCS work?
7. Do you feel that your current methods for doing quantitative
microscopy and analysis:
Exceed your research requirements
Match your research requirements
Are inadequate for your research needs
8. Respond to the statement:
"My capabilities match my research requrements for imaging but not
for
analysis"
Agree
Disagree
9. Please list the major instruments you are using, provide the name
and brand of the instruments. Use one line per instrument.
10. Give us an idea of what data management package you are using for
image management. For multiple packages, please list one per line.

High Content Analysis/Screening

3. Assay Systems

We would like to know something about the assays you run.
11. What are the general assays that are most important for your
needs. Please use one line per assay.
12. At what stage of discovery or development are the assays used?
and
what specifically are they used for:
To screen compounds, natural products or gene knock-outs?
Primary compound screening?
Secondary compound screening?
Tertiary screening?
Cell-based ADMET
Flow cytometry
Diagnostics/specialty testing
Other (please specify)
13. What cells are being used in your operations?

Primary cell lines
Cell lines
Live or fixed cells
Other (please specify)
14. Current throughput:
What is the assay format you use mostly? (select the highest use)

6 well
12 well
24 well
96-well
384-well
1536-well
chambered slide/coverslip dishes
Other (please specify)
15. Current throughput:
What is the approximate number of plates run per week? (Enter any
number)

6 well
12 well
24 well
96-well
384-well
1536-well
chambered slide/coverslip dishes
16. If performing compound screening:
How many compounds are evaluated in a run?
17. Do you buy Assay Kits?
yes
No
18. If you do buy assay kits, please list the 5 most important giving
a value of 1 (highest) to 5 lowest volume and write the manufacturer
beside the kit.
e.g 1 - TNF - Tidor Labs
1
2
3
4
5
19. As a general rule, do you buy most kits from the vendor of your
primary instruments?
yes
no
20. Do you alter assay protocols? (so we are asking a double question
here - if you do we want to know how you track the changes which is
the next question)
Yes
No
21. How do you track changes made in protocols if you do make
changes?
High Content Analysis/Screening

4. About your Instruments

Please give us some feedback on what brands of instruments you have
22. Indicate if you have instruments from any of the following:
Agilent
Amersham-Biosciences
Applied Biosystems
Arcturus
Atto Biosciences
Axon Instruments
BD Biosciences (Becton-Dickinson)
Blueshift Biotechnologies
BioImage
Cellomics
Cyntellect
Cytoprint
Evotec technologies
Vitra Bioscience
Q3dm
Other (please list one per line)

4. About your Instruments

Please give us some feedback on what brands of instruments you have
Please enter a comment.
22. Indicate if you have instruments from any of the following:
Agilent
Amersham-Biosciences
Applied Biosystems
Arcturus
Atto Biosciences
Axon Instruments
BD Biosciences (Becton-Dickinson)
Blueshift Biotechnologies
BioImage
Cellomics
Cyntellect
Cytoprint
Evotec technologies
Vitra Bioscience
Q3dm
Other (please list one per line)

4. About your Instruments

Please give us some feedback on what brands of instruments you have
Please enter a comment.
22. Indicate if you have instruments from any of the following:
Agilent
Amersham-Biosciences
Applied Biosystems
Arcturus
Atto Biosciences
Axon Instruments
BD Biosciences (Becton-Dickinson)
Blueshift Biotechnologies
BioImage
Cellomics
Cyntellect
Cytoprint
Evotec technologies
Vitra Bioscience
Q3dm
Other (please list one per line)

4. About your Instruments

Please give us some feedback on what brands of instruments you have
Please enter a comment.
22. Indicate if you have instruments from any of the following:
Agilent
Amersham-Biosciences
Applied Biosystems
Arcturus
Atto Biosciences
Axon Instruments
BD Biosciences (Becton-Dickinson)
Blueshift Biotechnologies
BioImage
Cellomics
Cyntellect
Cytoprint
Evotec technologies
Vitra Bioscience
Q3dm
Other (please list one per line)
High Content Analysis/Screening

4. About your Instruments

Please give us some feedback on what brands of instruments you have
Please enter a comment.
22. Indicate if you have instruments from any of the following:
Agilent
Amersham-Biosciences
Applied Biosystems
Arcturus
Atto Biosciences
Axon Instruments
BD Biosciences (Becton-Dickinson)
Blueshift Biotechnologies
BioImage
Cellomics
Cyntellect
Cytoprint
Evotec technologies
Vitra Bioscience
Q3dm
Other (please list one per line)

High Content Analysis/Screening

5. Informatics Issues

Informatics issues are complex. Please give us some ideas about how
we
can address this issue.
23. What do you see as the primary informatics issues concerning
imaging as a systems biology and drug discovery tool?
Data/Image storage
Data/Image retrieval/reuse
Automated Analysis
Visualization of data/images
Distribution of data/images between workgroups
Image format standards
Metadata standards
Other (please specify)
24. What problems do you see in your present informatics approaches?
25. Data generation rates:
How much HCS data do you currently have in storage (in GB)?
26. Over what time period was it generated?
       Years   Months
Length of time
27. How much data do you currently generate in a month?
1-100 MB
101-1000 MB
1-10 GB
11-100 GB

28. Regarding imaging data
How are the assays currently quantified? (e.g. automatically,
manually, 3rd party software?)
Number of features extracted per image?
High Content Analysis/Screening

6. Organizaton support

There are a number of organizations that work in somewhat related
areas. It would be useful to know if you are aware of some of these
organizations.
31. Identify which organizations you are a current member of. Use one
line per organization.
32. Are you aware of ISAC? (International Society for Analytical
Cytology)
Yes
No
33. Are you a member of ISAC
Yes
No
34. If ISAC were to establish a strong interest in the area of HCS,
would you find it more attractive to join and work within this
organization?
Yes
No
35. Do you think that it would be useful if there were an independent
facility that could test and evaluate instruments and software used
in
HCS, and provide high quality reviews?
Yes
No
Other (please specify)
36. Would your organization pay for such a service if it were
available?
Yes
No
High Content Analysis/Screening

7. Future HCS Web Page

We would like to know your opinions on existing support networks.
37. Do you think that existing Internet resources on HCS are adequate
for your current and future needs?
Yes
No
38. Please provide the address of web pages on HCS that you think are
useful. Use one line per address.
1
2
3
4
5
39. Indicate from the following the areas you would like to see on
the
new HCS website that is at www.cyto.purdue.edu/HCS
       Vital   Useful  Neutral OK      Low value
Email discussion group
Standards issues
Quality control
Protocol database
Software reviews
Hardware reviews
Reference database
Educational materials
Jobs/postition available
Jobs/postions needed
New product announcements
Advertising materials
Meeting/conferences directory
Short course announcements
Vendor directory

High Content Analysis/Screening

8. Now tell us about your concerns

We believe that there are a number of really complex issues that need
to be addressed. Please provide some insight into your own concerns.
40. Instead of us suggesting issues we would like you to suggest
them.
We suggest one line per issue.
High Content Analysis/Screening

9. Tell us about yourself

We would like to know who you are. This survey is being prepared by
an
academic institution (Purdue University) , therefore you can be
assured that the personal data will not be provided to 3rd parties.
We
will maintain the integrity and confidentiality of your answers. We
will invite you to join the discussion group that is being created.
41. Please supply your contact information. This will not be
distributed anywhere and will be kept confidential. If you don't
provide any contact details, we will not be able to keep in contact
with you as we develop an interest group.
Name
Company
Address1
Address2
City
Zip
State
Email

High Content Analysis/Screening

10. Thanks - that's it!

Thank you for participating in this survey. Our goal is to provide an
independent, but scholarly review of the area if HCS. We want to
assist people in this field and we need your help. Please let us know
how we can help. If you have comments please email them to me at the
address below.

As soon as you hit the "next" button below, you will be taken
directly
to the HCS page where you can join a discussion group in HCS. Please
send this page address to colleagues so that they can also take the
survey and subscribe to the group.

Addresses of subscribers will not be released to any 3rd party and
the
archive will only be available to subscribers.

Dr. J. Paul Robinson,
Purdue University
j...@flowcyt.cyto.purdue.edu
How many features are used to support "hit" identification?
How long do you keep the images after storage?
Do you store the images with associated annotation?
Do you derive additional data from stored images by performing
further
image analysis?
Do you use other algorithms?
Is access to satisfactory algorithms for image analysis a problem or
bottleneck?
Do you link the annotated image data to other data types such as the
results from other assays, chemical structures and/or toxicity data?
How do you currently extract information to support decision making?
How is knowledge generated from the data to support decision making?
29. Do you anticipate changing any of your processes in the near
future?
One or more platform components?
New instrument or new model?
Would this be the same vendor, different vendor?
Migrate HCS from secondary to primary screening?
30. Which of the topics discussed at CHI represents an area you feel
you should address to best improve your current HCS operations:
Assay development
Image acquisition
Image analysis
Image storage
Image retrieval
Data integration and mining to support
compound selection
Other

http://www.cyto.purdue.edu/hcs/

New Features coming
# E-mail Discussion Group
# Lecture on Cytomics and HCS (online version)
# Lecture on Cytomics and HCS (PowerPoint version - 14 Megabytes)
# Standards issues
# Quality control Issues
# Protocol database
#
Software*******************************************************************­
*****************************
reviews******************************************************WE GOT
OUR REVIEW!
# Hardware reviews
# Reference database
# Educational materials
# Jobs/postition available
# Jobs/postions needed
# New product announcements
# Advertising materials
# Meeting/conferences directory
# Short course announcements
# Related Societies & Organizations
# Journals and Publications of interest
# Vendor directory