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Cytometrysoftware, License Fees, Lab fees in Canada, and all to be     set by J Paul Robinson President ISAC CONGRESS 2008 through Purdue Cytometry     Mail List a must read the open records...GOOOGLE EXPOSES ALL

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Mitch Haynes - 25 Dec 2007 07:36 GMT
These data are already available on our website at Purdue are numbers
for over 80 core labs in the country
Re: Canadian Fee Survey
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From: J. Paul Robinson <jpr@flowcyt.cyto.purdue.edu>
Date: Sun Mar 18 2007 - 23:12:39 EDT
These data are already available on our website at Purdue
http://www.cyto.purdue.edu/flowcyt/labinfo/rates.htm  there are
numbers for over 80 core labs in the country  regards    paul
robinson      Kristin Chadwick wrote:  > Hello all,  >    > I run a
Flow Cytometry Core Facility at a Canadian academic institute.   > We
are currently evaluating our user fees for the upcoming fiscal year.
>    > If you run an academic flow cytometry core facility in Canada,
could you   > please indicate which of the following services you
provide, along with   > the associated hourly rates:  >    >   > 1)
Analyser  user operated  >   > 2)  Analyser  with Core operator
support  >   > 3)  Sorting  by Core operator  >   > 4)  Sorting, or
use of sorter for analysis (ie UV, multi-colour not   >
possible on Analyser)  user operated  >   > 5)  Training  >   > 6)
Data analysis on offline workstation  >   > 7)  Do you distinguish
between academic/institutional or   > non-academic/corporate users?
>   >    > I will provide a summary of this information once my survey
is   > complete. Thank you in advance for your assistance.  >   >
Sincerely,  >    > Kristin  >   >   > *Kristin Chadwick*  > *Manager*
> London Regional Flow Cytometry Facility  > 4th Floor, Room E4-07  >
Robarts Research Institute  > P.O. Box 5015, 100 Perth Drive  >
London, ON  Canada  N6A 5K8  > Phone: (519) 663-5777 x34042  > Fax:
(519) 663-3789  > kchadwick@robarts.ca <mailto:kchadwick@robarts.ca>
>   >       --   J. Paul Robinson  SVM Professor of Cytomics
Professor of Immunopharmacology & Biomedical Engineering  Director,
Purdue University Cytometry Laboratories  President, International
Society
for Analytical Cytology    Purdue University Cytometry Laboratories
Bindley Bioscience Center  1203 West State Street  Discovery Park,
Purdue University  West Lafayette, IN 47907-2057  Ph (765) 494 0757;
Fax (765) 494 0517  email: jpr@flowcyt.cyto.purdue.edu  www.cyto.purdue.edu
Join ISAC - www.isac-net.org    Change lives today  - www.cytometryforlife.org
Received on Mon Mar 19 11:18:02 2007
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03:12:00 E

.

*****************************************************************************
because I'm basically   too lazy to reply to each individual person
(sorry)) FLOW JO, PAUL J ROBINSON AND NIH GRANTS

The Daily Dongle: - 2 visits - Nov 25
Rant about FlowJo's goings-on. ... NIH Funding. Another thread from
Purdue worth posting: .... I saw this question on Purdue mailing list
earlier today. ...
flowjo.typepad.com/the_daily_dongle/2007/06/index.html - 33k - Cached
- Similar pages - Note this
The Daily Dongle: Current Affairs
Rant about FlowJo's goings-on. ... NIH Funding. Another thread from
Purdue worth posting:. We are calling upon you again during this
Independence Day ...
flowjo.typepad.com/the_daily_dongle/current_affairs/index.html - 27k
-
Cached - Similar pages - Note this
[ More results from flowjo.typepad.com ]
This page under construction
http://www.cyto.purdue.edu/hmarchiv/cytomail.htm. Flow cytometry
analysis software: ... http://www.niaid.nih.gov/reposit/tetramer/index.html.
Ebioscience ...
www.immunology.utoronto.ca/research/resources/msb/fc.htm - 27k -
Cached - Similar pages - Note this
The Daily Dongle: - 2 visits - Nov 25Rant about FlowJo's goings-
on. ... -the impact of cuts to grants already funded .... I saw this
question on Purdue mailing list earlier today. Since FlowJo ...
flowjo.typepad.com/the_daily_dongle/2007/06/index.html - 33k - Cached
- Similar pages - Note this
The Daily Dongle: Current Affairs - 12:10amRant about FlowJo's
goings-
on. ... For those of you not on Purdue, Joao Loureiro from Portugal
wrote: ... -the impact of cuts to grants already funded ...
flowjo.typepad.com/the_daily_dongle/current_affairs/index.html - 27k
-
Cached - Similar pages - Note this
Purdue Cytometry Mailing List: By Date - Nov 25Re: Physical
exhaustion
and immune system FlowJo Technical Support - Maciej Simm (Wed Mar 14
2007 - 17:41:46 ..... Travel grants for young investigators, ...
www.cyto.purdue.edu/hmarchiv/Current/thread.htm - 288k - Cached -
Similar pages - Note this
Purdue Cytometry Mailing List: By Date - 2 visits - Nov 25Re: Using
DIVA Exp Files in Flojo FlowJo Technical Support - Maciej Simm ......
Travel grants for young investigators, ImmunoRio2007
gharago...@sums.ac.ir ...
www.cyto.purdue.edu/hmarchiv/current/index.htm - 318k - Cached -
Similar pages - Note this
[ More results from www.cyto.purdue.edu ]
The Whitaker Foundation 1996 Annual Report - Grants ApprovedRESEARCH
GRANTS Auburn University Thomas S. Denney, Jr., .... State
University)
Becky Jo Ficek Rice University (B.S. Purdue University) Anne Marie
Findlay ...
www.bmes.org/WhitakerArchives/96_annual_report/grantsap.html - 33k -
Cached - Similar pages - Note this
Vision now reality in Purdue's Discovery ParkThe park's Birck Center
has awarded $2.4 million in start-up grants for nine of those new
faculty .... V. Jo Davisson, (7865) 494-5238, davis...@purdue.edu ...
www.purdue.edu/UNS/html3month/2004/040919.Rutledge.dispark.html - 28k
- Cached - Similar pages - Note this
Testimony of JO ANNE GOODNIGHTThe development of the miniaturized
flow
probe marks the first time that anyone has ... Funding provided by an
STTR Phase I grant funding resulted in the ...
www.hhs.gov/asl/testify/t010621.html - 26k - Cached - Similar pages -
Note this
This page under constructionFlow cytometry users: useful sites.
Scripps Research Institute Flow Cytometry Core Facility http://facs.scripps.edu/.
Purdue University Cytometry ...
www.immunology.utoronto.ca/research/resources/msb/fc.htm - 27k -
Cached - Similar pages - Note this
FACS Flow Cytometry FacilityUse of stand-alone workstation with
CellQuest and FlowJo software for data analysis ... Flow cytometry
users: useful sites http://facs.scripps.edu/. Purdue ...
www.immunology.utoronto.ca/CellSorter.htm - 23k - Cached - Similar

Note this EVEN HAVE YAHOOOOOOOOOO! ON THE LIST!
NEVER STOOOOOOOOD A CHANCE WITH OUT GOOOOOOOOOOOOOOOOOOOOOOOOOOGLE!
Links to other organizations and resources
Danish organizations
http://www.flowcytometri.dk/links.htm
   * Aarhus Universitet (University of Aarhus).
   * Biokemisk Forening (Danish Society for Biochemistry and
Molecular Biology).
         o BioZoom.
   * BioLogue.
   * Biotech Research and Innovation Centre (BRIC).
   * Danish Research School in Molecular Cancer Research (MCRS).
   * Dansk Hæmatologisk Selskab.
   * Dansk Institut for Ekstern Kvalitetssikring (Danish Institute
for External Quality Assurance for Laboratories in Health Care,
DEKS).
   * Dansk Medicinsk Selskab (Danish Medical Society, DMS).
   * Dansk Selskab for Allergologi (Danish Society for Allergology).
   * Dansk Selskab for Cancerforskning (Danish Association for
Cancer
Research, DSCF).
   * Dansk Selskab for Cyto- og Histokemi.
   * Dansk Selskab for Klinisk Immunologi.
   * Dansk Selskab for Klinisk Mikrobiologi (Danish Society for
Clinical Microbiology, DSKM).
   * Dansk Selskab for Medicinsk Genetik.
   * Dansk Selskab for Patologi (DSP).
   * Dansk Selskab for Patologisk Anatomi og Cytologi (Danish
Society
for Pathological Anatomy and Clinical Cytology, DSPAC).
   * Danske bioanalytikere (dbio).
   * Immunologisk Selskab (Danish Society of Immunology).
   * Kræftens Bekæmpelse (Danish Cancer Society).
   * Københavns Universitet (University of Copenhagen).
         o Akademisk Kalender (Sundhedsvidenskabelige Fakultet).
   * SyddanskUniversitet (University of Southern Denmark).
International and other national or regional organizations
   * Association Francaise de Cytométrie (AFC).
   * Cytorelay Node, Max Planck Institute of Biochemistry,
Martinsried.
         o Concepts and history....
   * Diagnostic Molecular Imaging (DiMI).
   * EQUALIS (External quality assurance in laboratory medicine in
Sweden).
   * European Association for Cancer Research.
   * European Molecular Biology Organization (EMBO).
   * European Molecular Imaging Laboratories (EMIL).
   * European School of Haematology (ESH).
   * European Society for Clinical Cell Analysis (ESCCA) - formerly
European Working Group for Clinical Cell analysis (EWGCCA).
   * European Working Group for Clinical Cell analysis (EWGCCA) -
has
changed into European Society for Clinical Cell Analysis (ESCCA).
         o Journal of Biological Regulators (proceedings of some
EWGCCA meetings).
   * Flow Cytometry on the Web (Salk)
   * FlowcytometryUK
   * Flow Informatics and Computational Cytometry Society FICCS).
   * Human Cytome Project.
   * German Society for Cytometry (Deutsche Gesellshaft für
Zytometrie, DGfZ).
   * International Society for Analytical Cytology (ISAC).
         o ISAC publications:
               + Cytometry, Part A and B.
               + Current Protocols in Cytometry.
               + ISAC E-Newsletter.
   * International Society for Cellular Oncology (ISCO).
         o Cellular Oncology.
   * Marie Curie - Genome Architecture in Relation to Disease (MC-
GARD).
   * National Flow Cytometry Resource (LANL), Los Alamos National
Laboratory.
   * Nordic Immunohistochemical Quality Control (NordiQC).
   * Norsk Flowcytometri Forening (NFCF).
   * Purdue University Cytometry Laboratories (PUCL).
         o E-mail Archive.
   * Royal Microscopical Society (RMS) - Cytometry Section.
   * Svensk Förening för Flödescytometri (SFF).
   * Stichting Immunotypering Hematologische Oncologie in Nederland
(SIHON).
   * TSRI Flow Cytometry Core Facility (Scripps).
   * UKNEQAS (United Kingdom National External Quality Assessment
Service).
Internet search engines & Libraries
   * AltaVista.
   * Answers.com.
   * a9.
   * Biocrawler.
   * BioMed Central.
   * BioSite.
   * BiotechSweden.
   * CD Molecules (PROW list).
   * Cytokine Family Database.
   * Dagens Medicin.
   * EuroSciCon (European Scientific Conferences).
   * Free Medical Journals.
   * Google.
         o Google Scholar.
   * Highwire.
   * HTML.dk - Guide til webudvikling.
   * Kvasir.
   * Københavns Universitetsbibliotek (KUB Nord, tidligere DNLB,
Fakultetsbibliotek for Natur- og Sundhedsvidenskaberne).
   * Max Planck Society - Electronic Journals Library.
   * MedBioWorld.
   * Merriam-Webster OnLine (dictionary - thesausus).
   * Nature Publishing Group (science updates).
   * PubMed (NCBI Entrez, Medline).
   * Scirus.
   * Web of Science.
   * WebCrawler.
   * Wikipedia.
   * WWW Virtual Library.
   * Yahoo.
Biosafety
   * Arbejdstilsynet (Danish Working Environment Authority).
   * CDC Office of Health and Safety: Biosafety.
   * ISAC website, see Resources and Standards.
   * OSHA (U.S. Dept. of Labor, Occupational Safety & Health
Admin.).
Hardware and software
   * Cytometry products
         o Corporate Members of DSFCM
         o Commercial Web Sites - Cytometry suppliers at PUCL
website.
   * Fluorochromes
         o Handbooks
               + Shapiro: Practical flow cytometry, 4th ed.
               + Haugland: Handbook of Fluorescent Probes and
Research Products, 10th ed.
               + Robinson et al.: Current Protocols in Cytometry.
         o Spectrum viewers
               + BD's Fluorescence Spectrum Viewer.
               + Invitrogen's Fluorescence Spectra Viewer.
         o Fluorescence conjugation
   * Guidelines
         o Resources and Standards at ISAC website.
         o Literature.
   * Software
         o Resources at ISAC website.
         o Catalog of Free Flow Cytometry Software at PUCL website.
         o WinMDI, free off-line software for analysis of flow
cytometric data.
Revised, 20 November 2007 /JKL
Quick Links:
Flow Cytometry, cytometry,Confocal Microscopy, multiphoton
microscopy,
Image Analysis, ...
We present cytometry and confocal microscopy education and research
material, maintain the cytometry email archive,and links to cytometry
web sites and suppliers worldwide. Our goal is to share high-quality
information ❈
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Purdue Cytometry Mailing List: Free WinList Update Available
... Purdue Cytometry Mailing List: Free WinList Update Available ...
Mark E. Munson Sales Manager Verity Software House, Inc. 45A
Augusta ...
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02 Apr 03
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Purdue Cytometry Mailing List: Extracting data from FACS measurements
... Purdue Cytometry Mailing List: Extracting data from FACS
measurements ... Mark E. Munson Sales Manager Verity Software House,
Inc. 45A Augusta ...
http://www.cyto.purdue.edu/hmarchiv/2003/0491.htm - 6.1KB
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18 Mar 03
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Purdue Cytometry Mailing List: Course Announcement - 25th ANNUAL
COURSE IN FLOW CYTOMETRY
... Purdue Cytometry Mailing List: Course Announcement - 25th ANNUAL
COURSE IN FLOW CYTOMETRY ... Mark E Munson Verity Software House,
Inc.
PO Box 247 ...
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23 Oct 01
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Purdue Cytometry Mailing List: New Purdue DVD - Cytometry Volume 10
... Purdue Cytometry Mailing List: New Purdue DVD - Cytometry
Volume ... Optical, Macs Miltenyi Biotec, SouthernBiotech, Verity
Software Accuri, Chroma, Coherent, Cyopeia, Duke ...
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17 Nov 07
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Purdue Cytometry Mailing List: New England Cytometry Meeting
Registration
... Purdue Cytometry Mailing List: New England Cytometry Meeting
Registration ... speakers this year: ... C. Bruce Bagwell, MD, Ph.D.
-
Verity Software House "Probability State Models: A new paradigm for
cytometry analysis ...
http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1382.htm - 9.1KB
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18 Sep 07
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Purdue Cytometry Mailing List: By Thread
... Purdue Cytometry Mailing List: By Thread ... PML protein Jan
Nelson (Wed Dec 16 1998 - 16:56:58 EST) ... Permeabilization and
Anti-
Coagulants Vincent Falco (Wed Dec 16 1998 - 09:07:16 EST) ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Thread
... Purdue Cytometry Mailing List: By Thread ... Sales position in
Germany Brian Hall (Thu Dec 18 1997 - 12:23:18 EST) ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Thread
... Purdue Cytometry Mailing List: By Thread ... Summarize "Free
software" Dorte Christiansen (Fri Dec 12 2003 - 07:45:44 EST) ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Thread
... Purdue Cytometry Mailing List: By Thread ... staining
DARZYNKIEWICZ ZBIGNIEW (Wed Dec 20 2000 - 18:42:40 EST) ... CD45
expression on ADP-activated murine platelets Gregor Rothe (Thu Dec 21
2000 - 07:29:44 EST) ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Subject
... Purdue Cytometry Mailing List: By Subject ... 2444 messages
sorted
by: [ author ] [ date ] [ thread ] [ attachment ] Other mail
archives ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Thread
... Purdue Cytometry Mailing List: By Thread ... is good enough?
Uriel
TK (Tue Dec 14 2004 - 16:04:58 EST) ... RE: How close compensation is
good enough? Gerstein, Rachel (Thu Dec 16 2004 - 07:07:48 EST) ...
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30 Jan 07
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Purdue Cytometry Mailing List: Applications Specialist - iCyt -
... Northwest Cytometry Users Group and Cascade Cytometry Users Group
are pleased to announce the 2007 NW Regional Cytometry Meeting,
which ... Tree Star, Union Biometrica, and Verity Software House. ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Date
... Purdue Cytometry Mailing List: By Date ... Re: Cell-Dyn 4000
Howard Shapiro (Wed Dec 18 2002 - 21:51:24 EST) ... pluronic acid
g...@biocfarm.unibo.it (Thu Dec 19 2002 - 07:07:54 EST) ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Thread
... Purdue Cytometry Mailing List: By Thread ... free FACS analysis
software Wolfraim, Lawrence (NCI) (Wed Dec 19 2001 - 10:28:23
EST) ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Thread
... Purdue Cytometry Mailing List: By Thread ... WinMDI software -
long filename problem WAS Doubts About WinMDI software Alan Bishop
(Thu Dec 01 2005 - 19:07:54 EST) ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Author
... Purdue Cytometry Mailing List: By Author ... 2 flow cytometry job
openings at Biogen (Thu May 23 2002 - 07:09:37 EST) ...
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30 Jan 07
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Purdue Cytometry Mailing List: Re: DIVA Software on MAC Intel Co
... Purdue Cytometry Mailing List: Re: DIVA Software on MAC Intel
Co ... screen display issue, that made it difficult to read the text.
Verity provided us with a patch for this in remarkably little
time ...
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15 Dec 06
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Purdue Cytometry Mailing List: Re: Log-like transforms
... Purdue Cytometry Mailing List: Re: Log-like transforms ...
purposes. > > > > Bruce > > > > C. Bruce Bagwell MD, Ph.D. > >
President > > Verity Software House > > 45A Augusta Road > > PO Box
247 > > Topsham, ME 04086 ...
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22 Mar 06
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Purdue Cytometry Mailing List: RE: Software for merging FSC 3 fi
... Purdue Cytometry Mailing List: RE: Software for merging FSC ...
J.
Herbert Technical Support Manager Verity Software House, Inc. PO Box
247 ...
http://www.cyto.purdue.edu/hmarchiv/2005/0217.htm - 6.6KB
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03 Feb 05
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Purdue Cytometry Mailing List: RE: Software recommendations for
... Purdue Cytometry Mailing List: RE: Software recommendations
for ... Mark E. Munson Sales Manager Verity Software House, Inc. 45A
Augusta ...
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25 Oct 04
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Purdue Cytometry Mailing List: Analysis Software
... Purdue Cytometry Mailing List: Analysis Software ... Donald J.
Herbert Technical Support Manager Verity Software House, Inc. PO Box
247 45A Augusta Road Topsham, ME, USA 04086 ...
http://www.cyto.purdue.edu/hmarchiv/2004/1196.htm - 7.1KB
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30 Jun 04
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Purdue Cytometry Mailing List: Re: Software to overlay dot plots for
analysis
... Purdue Cytometry Mailing List: Re: Software to overlay dot plots
for analysis ... Quest for OSX - a new release from BD 4. WinList -
Verity Software House 5. FCS Express - from www.denovosoftware.com
...
http://www.cyto.purdue.edu/hmarchiv/2003/0979.htm - 5.6KB
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28 May 03
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Purdue Cytometry Mailing List: RE: Kolmogorov-Smirnov (Is there a
better solution?)
... Purdue Cytometry Mailing List: RE: Kolmogorov-Smirnov (Is
there ... Bruce Bagwell, MD, Ph.D. Verity Software House, Inc.
Published: Clinical Immunology ...
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18 Apr 03
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Purdue Cytometry Mailing List: Re: non-radioactive LPA and responses
to query on LPAs
... PKH Proliferation assays by flow cytometry Organization: Verity
Software House ... dartmouth.edu (Alice L. Givan) ModFit software
from
Verity has a "Proliferation Wizard" that ...
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20 Aug 02
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Purdue Cytometry Mailing List: RE: query on LPAs: replacing tritiated
thymidine with flow
... Purdue Cytometry Mailing List: RE: query on LPAs: replacing ...
J.
Herbert Technical Support Manager Verity Software House, Inc. PO Box
247 ...
http://www.cyto.purdue.edu/hmarchiv/2002/1649.htm - 9.5KB
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13 Aug 02
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Purdue Cytometry Mailing List: Re: Last Message on Purdue Cytometry
CD-
ROM Vol 6
... Cytometry Mailing List <cytome...@flowcyt.cyto.purdue.edu> ...
Associates, Inc; Spherotech, Tree Star, Verity Software, Spherotech,
Tree Star, Verity Software. ...
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03 Apr 02
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Purdue Cytometry Mailing List: UV excitable dyes for surface
phenotyping - ELF-97?
... Purdue Cytometry Mailing List: UV excitable dyes for surface
phenotyping - ELF-97? ... RE: Bacteria sorting?" ... Previous
message:
VSH - Tech Support: "Announcing User Forum at Verity Software House,
Inc." ...
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12 Mar 02
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Purdue Cytometry Mailing List: 25th ANNUAL COURSE IN FLOW CYTOMETRY:
BOWDOIN COLLEGE, ...
... Purdue Cytometry Mailing List: 25th ANNUAL COURSE IN FLOW
CYTOMETRY: BOWDOIN COLLEGE, BRUNSWICK MAINE - JUNE 2002 ... Mark E
Munson Verity Software House, Inc. PO Box 247 Topsham, ME 04086 Tel:
(207) 729-6767 x105 ...
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22 Jan 02
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Purdue Cytometry Mailing List: Re: G4's will run OS8.6 (ours sur
... Purdue Cytometry Mailing List: Re: G4's will run OS8.6 (ours
sur ... evidenced by the hundreds of labs running their XL software
under > DOS (yuk!). Maybe Verity or TreeStar will make some inroads
if
we demand ...
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13 Jan 00
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Purdue Cytometry Mailing List: G4's will run OS8.6
... Purdue Cytometry Mailing List: G4's will run OS8.6 ... edge
software, evidenced by the hundreds of labs running their XL software
under DOS (yuk!). Maybe Verity or TreeStar will make some inroads if
we demand state of ...
http://www.cyto.purdue.edu/hmarchiv/2000/0140.htm - 7.4KB
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13 Jan 00
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Purdue Cytometry Mailing List: RE: flow cytometry course in June
... Purdue Cytometry Mailing List: RE: flow cytometry course in
June ... Brunswick Maine. Course organizer is C. Bruce Bagwell. I
think that Verity Software may be sponsoring it, as their information
is on some of ...
http://www.cyto.purdue.edu/hmarchiv/1998/0905.htm - 5.1KB
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10 Apr 98
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Purdue Cytometry Mailing List: RE: utility software to convert fcs1.0
to fcs2.0
... Purdue Cytometry Mailing List: RE: utility software to
convert ...
PC, I suggest you contact Verity Software (207-729-6767) . I
quickly ...
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27 Mar 98
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Purdue Cytometry Mailing List: RE: utility software to convert fcs1.0
to fcs2.0
... Purdue Cytometry Mailing List: RE: utility software to convert
fcs1.0 to fcs2.0 ... Since you are using the PC, I suggest you
contact
Verity Software (207-729-6767) .  I quickly tried this function this
AM ...
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27 Mar 98
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Purdue Cytometry Mailing List: FW: FCS file transfer between BD Diva
(PC) and CellQuest ( ...
... Purdue Cytometry Mailing List: FW: FCS file transfer between ...
morphosys.com> Date: Thu Nov 08 2007 - 07:14:16 EST ...
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10 Nov 07
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Purdue Cytometry Mailing List: RE: 2008 NW Regional Cytometry Meeting
announcement
... Purdue Cytometry Mailing List: RE: 2008 NW Regional Cytometry
Meeting announcement ... Bioscience, Miltenyi Biotec, MDA Analytical
Technologies, Partec, Tree Star, Union Biometrica, and Verity
Software
House. ...
http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1644.htm - 6.5KB
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03 Nov 07
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Purdue Cytometry Mailing List: New England Cytometry Meeting
Registration Reminder
... Purdue Cytometry Mailing List: New England Cytometry Meeting
Registration Reminder ... speakers this year: ... C. Bruce Bagwell,
MD, Ph.D. - Verity Software House "Probability State Models: A new
paradigm for cytometry analysis ...
http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1458.htm - 8.6KB
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04 Oct 07
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Purdue Cytometry Mailing List: New England Cytometry Annual Meeting
Registration and Hotel ...
... Purdue Cytometry Mailing List: New England Cytometry Annual
Meeting Registration and Hotel Information ... year: ... C. Bruce
Bagwell, MD, Ph.D. - Verity Software House "Probability State Models:
A new paradigm for cytometry ...
http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1411.htm - 8.8KB
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23 Sep 07
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Purdue Cytometry Mailing List: SUMMARY of Responses: Biexponential
Plots and CSFE
... Purdue Cytometry Mailing List: SUMMARY of Responses:
Biexponential
Plots and CSFE ... FCS3.0 digital data was presented by Mark Munson
of
Verity Software House (makers of Modfit). Thanks Mark, it worked
great ...
http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1403.htm - 8.1KB
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22 Sep 07
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Purdue Cytometry Mailing List: Invitation for Vendor Sponsorship for
the New England ...
... to announce the New England Cytometry Users Group Fall meeting;
Current Concepts in Flow and Image Cytometry which will be held
October ... Bruce Bagwell, MD, Ph.D. - Verity Software House http://www.vsh.com
...
http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1175.htm - 8.6KB
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11 Aug 07
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Purdue Cytometry Mailing List: RE: CFSE graphs (UNCLASSIFIED)
... Verity's MODFIT software has a very nice tool for
proliferation ... Stelekati [mailto:e...@fz-borstel.de] >>>Sent:
Monday, March 26, 2007 6:58 AM >>>To: Cytometry Mailing List
>>>Subject: CFSE graphs >>> >>>Dear all, >>>I want to ...
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30 Mar 07
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Purdue Cytometry Mailing List: Applications Specialist - iCyt -
... Purdue Cytometry Mailing List: Applications Specialist - iCyt ...
Sponsored by: The National Flow Cytometry Resource (NFCR) and Verity
Software House ...
http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0214.htm - 8.4KB
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30 Jan 07
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Purdue Cytometry Mailing List: By Date
... Purdue Cytometry Mailing List: By Date ... RE: DIVA SOFTWARE ON
INTEL CORE 2 DUO PROCESSOR Guy Hermans ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Author
... Re: Free Flow Cytometry Analysis software? (Fri Apr 14 2006 -
02:56:18 EDT) ... CORRECTION- Chesapeake Cytometry Consortium (Thu
Sep
07 2006 - 12:20:05 EDT) ...
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30 Jan 07
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Purdue Cytometry Mailing List: RE: Partec CyFlow ML? (was Re:
Analyzers)
... Purdue Cytometry Mailing List: RE: Partec CyFlow ML? (was Re:
Analyzers) ... Received on Fri Nov 30 18:07:07 2007 ...
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01 Dec 07
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Purdue Cytometry Mailing List: RE: Cytometry software for linux
... Purdue Cytometry Mailing List: RE: Cytometry software for
linux ... ugr.es] > Sent: Monday, September 10, 2007 12:28 PM > To:
Cytometry Mailing List > Subject: Cytometry software ...
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15 Sep 07
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Purdue Cytometry Mailing List: Re: Summary and replies on PCH101
... Purdue Cytometry Mailing List: Re: Summary and replies on
PCH101 ... From: clare Rogers <rog...@med.umich.edu> Date: Fri Sep 07
2007 - 17:04:21 EDT ...
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11 Sep 07
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Purdue Cytometry Mailing List: For Sale BD FACSVantage with DiVa
option
... Purdue Cytometry Mailing List: For Sale BD FACSVantage with DiVa
option ... ml tubes -- PC and Mac computers with all original BD
software (DiVa [PC] and CELLQuest [Mac.]) NOTE: I will only
guarantee ...
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01 Sep 07
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Purdue Cytometry Mailing List: BD bead array software and FC500s
... Purdue Cytometry Mailing List: BD bead array software and
FC500s ... From: Stephen Taylor - Porton Down
<Stephen.Tay...@hpa.org.uk> Date: Thu Aug 16 2007 - 07:01:09 EDT ...
http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1204.htm - 6.0KB
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18 Aug 07
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Purdue Cytometry Mailing List: RE: Upgrading Computers and BD
Software
... Purdue Cytometry Mailing List: RE: Upgrading Computers and BD
Software ... utoronto.ca> > To: Cytometry Mailing List
<cytome...@flowcyt.cyto.purdue.edu> > Date: 8/13/2007 ...
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17 Aug 07
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Purdue Cytometry Mailing List: FACSort for sale.
... Purdue Cytometry Mailing List: FACSort for sale. ... Blue laser
and sorting option (bought in Oct. 1997) with Cellquest software
installed in Power Macintosh computer. The laser current is around
5.70Amps ...
http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0616.htm - 5.6KB
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02 May 07
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Purdue Cytometry Mailing List: By Date
... Purdue Cytometry Mailing List: By Date ... Summarize "Free
software" Dorte Christiansen (Fri Dec 12 2003 - 07:45:44 EST) ...
http://www.cyto.purdue.edu/hmarchiv/2003/ - 385.3KB
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30 Jan 07
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Purdue Cytometry Mailing List: By Author
... Purdue Cytometry Mailing List: By Author ... Flow facility, again
(Fri Dec 04 1998 - 03:50:07 EST) ... Flow facility (Mon Nov 23 1998 -
07:07:29 EST) ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Date
... Purdue Cytometry Mailing List: By Date ... PML protein Jan Nelson
(Wed Dec 16 1998 - 16:56:58 EST) ... Permeabilization and Anti-
Coagulants Vincent Falco (Wed Dec 16 1998 - 09:07:16 EST) ...
http://www.cyto.purdue.edu/hmarchiv/1998/index.htm - 491.8KB
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30 Jan 07
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Purdue Cytometry Mailing List: By Subject
... Purdue Cytometry Mailing List: By Subject ... owner-cytometry
(Wed
Jun 07 2006 - 10:19:50 EDT) ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Subject
... Purdue Cytometry Mailing List: By Subject ... Rosie Clarke (Fri
Feb 07 2003 - 07:14:11 EST) ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Author
... Re: Réf. : Free software? (Fri Dec 19 2003 - 22:39:46 EST) ... UV
vs Krypton UV (Thu Feb 20 2003 - 19:09:39 EST) ... RE: malignant
plasma cell detection by flow cytometry (Fri Feb 07 2003 - 19:48:43
EST) ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Date
... Purdue Cytometry Mailing List: By Date ... Thu Dec 16 2004 -
07:07:48 EST ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Author
... Purdue Cytometry Mailing List: By Author ... Re: OS9+Cellquest
(Tue Mar 07 2000 - 08:07:34 EST) ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Date
... Purdue Cytometry Mailing List: By Date ... Thu Dec 21 2000 -
07:29:44 EST ...
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Purdue Cytometry Mailing List: By Subject
... Purdue Cytometry Mailing List: By Subject ... JSC-SD) (Wed Sep 06
2000 - 08:37:10 EST) ... Walker, Don (SEA) (Thu Aug 31 2000 -
14:09:16
EST) ... Palkowetz, Kimberly H. (Tue Aug 29 2000 - 07:49:22 EST) ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Date
... Purdue Cytometry Mailing List: By Date ... Sales position in
Germany Brian Hall (Thu Dec 18 1997 - 12:23:18 EST) ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Author
... Purdue Cytometry Mailing List: By Author ... Re: Elite Windows
Software (Fri Aug 15 1997 - 21:44:56 EST) ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Author
... Purdue Cytometry Mailing List: By Author ... RE: Open Source Flow
Cytometry Data Analysis Software (Sun Nov 11 2001 - 16:36:29 EST) ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Date
... Purdue Cytometry Mailing List: By Date ... Re: free FACS analysis
software Paula Lavery (Wed Dec 19 2001 - 15:05:30 EST) ...
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30 Jan 07
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Purdue Cytometry Mailing List: By Subject
... Purdue Cytometry Mailing List: By Subject ... 2902 messages
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CONTROL OF SOFTWARE AND CONTRACTS

Enjoy yourselves,  Geoff  --  Geoffrey Osborne  Director of Flow
Cytometry,  The Queensland Brain Institute /Australian Institute for
Bioengineering   and Nanotechnology,  The University of Queensland,
St Lucia, QLD, 4072,
Australia  Ph (61) 07 33469912  email g.osborne@uq.edu.au
Received on Tue Mar 20 10:18:03 2007
Internet time scheduling system
•    This message: [ Message body ] [ More options ]
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thread ] [ Replies ]
From: Geoffrey Osborne <g.osborne@uq.edu.au>
Date: Tue Mar 20 2007 - 00:13:11 EDT
Hi All,  Due to the large amount of interest generated by my previous
post
regarding the FREE   multi language instrument time scheduling system
we use here, (and because I'm basically   too lazy to reply to each
individual person (sorry)), please find have a look at this  link
http://mrbs.sourceforge.net/index.html  which shows you where the
software comes from, and look here    http://mrbs.sourceforge.net/sshots.html
for some screen shots, which are about 7 years old...    Get your
local computer people to install it. I had my local IT guy modify it
to enable   booking "lock-out" at a configurable time prior to the
appointment time. This  modification is   available if I chase him up
to find out what he did.....    Enjoy yourselves,  Geoff  --  Geoffrey
Osborne  Director of Flow Cytometry,  The Queensland Brain Institute /
Australian Institute for Bioengineering   and Nanotechnology,  The
University of Queensland,  St Lucia, QLD, 4072,
Australia  Ph (61) 07 33469912  email g.osborne@uq.edu.au
Received on Tue Mar 20 10:18:03 2007
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mice"
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Institute"
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system"
•    Reply: Michie, John, Dr : "RE: Internet time scheduling system"
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Subject ] [ By Author ] [ By messages with attachments ]
This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 -
03:12:00 EST
________________________________________

I have    > arrived to the conclusion this is due to faulty log-amp
behavior    > causing deviations from linearity. Certainly, this has
important    > consequences for our results on that machine!
Re: 2 machines, 2 compensation troubles.
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From: Howard Shapiro <hms@shapirolab.com>
Date: Tue Mar 27 2007 - 19:29:40 EDT
Uriel Trahtemberg wrote-    >  > As usual, when confronted with
trouble I turn to you for help. this    > concerns two machines with
similar problems.  >  > 1) The FACScalibur in our lab. I performed a
titration experiment    > for Sytox Green and when checking the
compensation needed for it to    > work with other stains, saw the
results shown in the pdf "SytoxG    > linearity". I have attached one
of the files (ApoPMN.NucAcTit.   > 1mar07.021) if you want to check it
out. As you can see, instead of    > being a nice distribution on the
X axis, it looks like a waving    > serpent! On close inspection, the
waviness can already be seen in    > the uncompensated FL1 vs. FL2
plot. After long thought, I have    > arrived to the conclusion this
is due to faulty log-amp behavior    > causing deviations from
linearity. Certainly, this has important    > consequences for our
results on that machine!
In order to confirm    > my findings I exported the FCS data to
excel, sorted the data    > according to the FL1 intensity and did a
FL1/FL2 plot. After   > accounting for statistical noise, the mean
ratio should stay   > constant - after all, that is the rationale of
compensation! As you    > can see from the "SytoxGreen.xls" plots,
that is hardly the case.  > I turn to you to ask for alternative
explanations and possible    > solutions. Is this kind of problem
repairable? how easily? We have    > a service contract with BD for
that machine, so would I be right to    > assume they should fix it?
What kind of tests would you suggest to    > confirm and/or refine my
findings?  > PS: SytoxGreen is NOT metachromatic AFAIK.    I'm feeling
lazy so I'll only comment on the first "problem", in    which the
reasons for replacing log amps in newer cytometer designs    become
apparent.    See p. 202 of Practical Flow Cytometry and the middle
panel of
Figure    4-57 (you can also go to www.analog.com and download
AD8307.pdf,    which is a data sheet on one of the newer log amp
modules). A log amp    is not a log table. The log amps used in most
flow cytometers are    made up of multiple modules and exhibit
linearity curves that are    wavy even in the range in which they
perform best. The typical   deviation from peak to trough over that
range is plus or minus 0.5    dB. Do the math and you will find that
is plus or minus 6 per cent,    if you're talking voltage. Plus or
minus 6 per cent is fine if you're    measuring immunofluorescence,
where other biologic and instrumental    factors typically contribute
much more to variance than does the    inherent, built-in, not-
correctable-by-replacing-parts inaccuracy of    the log amp. If you
measure nucleic acid stains, which are    stoichiometric, with a log
amp, you pretty much get a picture of the    log amp's nonlinearity
curve. The solution to the problem is to    measure
such stains using linear electronics. Most flow cytometer
manufacturers have now opted to build high dynamic range linear
electronics that typically allow accurate measurement over a four-
decade range; the resulting data are digitized to enough bits'
precision to allow conversion between linear and log scales to be
done digitally. It is, however, worth noting that Cytomation/Dako
and    Cytopeia cytometers do use log amps, converting the log data
with    high-resolution ADCs to allow digital linear-log conversion
for    compensation purposes, and the users of these cytometers are
not, in    general, bothered by the inaccuracy - unless, of course,
they try to    do something like measure DNA on a log scale.    -
Howard
Received on Wed Mar 28 13:58:00 2007
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Cell Cycle on FACSAria
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From: Katherina Garcia Garcia <kgargar@upo.es>
Date: Mon Mar 26 2007 - 10:00:57 EDT
Hello!    We have problems to see cell cycle on Aria. Sometimes we see
the 2 peaks but most of the  times we can4t.  We see the same sample
on Calibur and Coulter and the cell cycle is very good, but in  Aria I
can4t distinguish the peaks.    I am using the right filter and I pass
the sample at a very slow speed. I use PI-A vs
PI-W to exclude doublets but I can4t do it because is imposible to
distinguish wich dots  are single or doblets cells.    Any ideas?
Thanks in advance.    Katherina Garcma Garcma  Ticnico de Microscopma
y Citometrma  kgargar@upo.es  Centro Andaluz de Biologma del
Desarrollo  Consejo Superior de Investigaciones Cientmficas
Universidad Pablo Olavide  Ctra. Utrera km1  41013 Sevilla  Espaqa
Received on Tue Mar 27 12:18:00 2007
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cloning but I had a hard time setting up the Aria
cell line sorting on Aria
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From: Julie Bertout <julie.bertout@ibl.fr>
Date: Wed Mar 28 2007 - 10:03:04 EDT
Hello,    We would like to sort cell line transfected with GFP in
order to do a   cloning but I had a hard time setting up the Aria...
No problem to sort
lymphocytes or eosinophils from blood or spleen in   high speed
sorting but the medium and low sorting required for cell line
sorting are difficult to set up.    Does anybody sort transfected cell
line ? what conditions do you use :   concentration of cells? events/s
recommended? amplitude usually used?   pressure (BD told me sometimes
you have to play a little bit with it...)?    Thanks for your
answers.    Julie Bertout  cytometry lab  Institut Pasteur de Lille  1
rue du professeur Calmette  59800 Lille  France
Received on Wed Mar 28 13:18:00 2007
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From: McCloskey, Thomas <thomasm@nshs.EDU>
Date: Tue Mar 27 2007 - 14:01:06 EDT
A powerful way to analyze CFSE data is to calculate "precursor
frequency" or the % of the starting population which divided in
response  to the stimulus.  Some software can do this automaticaally
[such as  Flowjo].  If you have discrete peaks, you can also calculate
it  manually.  The idea is that in the one division peak, two cells
came  from one starting cell.  In the two division peak, four cells
came from  one original cell, and so on.  You end up with the number
of cells which  divided and by comparing to those cells which did not
divide [the first  peak or no division peak] , byou now have the
precursor frequeecncy for  that stimulus.     Good luck,  Tom
************************************************************************
*****  Thomas W. Mc Closkey, Ph. D.  Assistant Investigator, Center
for Immunology & Inflammation  The Feinstein Institute for Medical
Research, North
Shore University  Hospital  Assistant Professor of Pediatrics, New
York University School of  Medicine  Biomedical Research Center, 350
Community Drive  Manhasset, Long Island, New York 11030  ph:
516-562-4844 [office], 516-562-1084 [lab]
************************************************************************
*****        >>>-----Original Message-----  >>>From: Erietta Stelekati
[mailto:este@fz-borstel.de]   >>>Sent: Monday, March 26, 2007 6:58 AM
>>>To: Cytometry Mailing List  >>>Subject: CFSE graphs  >>>  >>>Dear
all,  >>>I want to compare 3 pictures of CFSE proliferation. I can
see   >>>differences in the percentage of cells in each pick. But
how   >>>do I put   >>>all the data together and decide if it is a
statistically   >>>significant   >>>result? I can make a graph with
the percentage of cells in   >>>each pick,   >>>but then
I have to compare the percentages in each one of   >>>the 5-6
picks,   >>>which makes my point (more or less proliferation) not
easy   >>>to deliver...   >>>Should I , for example, compare only the
"non-proliferating"   >>>cells (=   >>>first pick) with all the others
together? Or only the "most   >>>proliferating cells" (= last pick)?
>>>Thank you in advance for any suggestions!  >>>Erieta Stelekati
>>>  >>>
---------------------------------------------------------------------------------
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________________________________________
Beckman staining HIV
CD8beta stain probs
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From: Geza Paukovics <paukovic@burnet.edu.au>
Date: Thu Mar 22 2007 - 19:37:19 EDT
>Hello Flow-ers,      >We are having problems with the CD8betape mAb from Beckman staining HIV+   >whole blood before lysis and permeabilisation and staining with mAb   >against cytokines. We observe the following poor staining intermittently,   >and can't find a cause. Intriguingly, we do not observe this poor staining   >on hepatitis B infected or uninfected
individuals. We observed poor   >CD8beta staining in two HIV+
patients and when exposed to light during the   >permeabilisation
step, the CD8betape mean flourescence of the positive   >population
increased with exposure to light. We repeated this experiment   >and
did not observe the poor CD8beta staining, and the mean fluorescence
>of the positive population did not increase with exposure to light.
Does   >anyone have any suggestions as to what may be causing this?
>  >could replies be posted to:    Louisa.Mathias@med.monash.edu.au
fanx for your time and attention...  cheers  Gez              Geza
Paukovics B.MLS.  Senior Research Assistant  The Macfarlane Burnet
Institute for Medical Research and Public Health  Site address:
Alfred Medical Research & Education Precinct (AMREP),  corner Punt &
Commercial Roads, Prahran 3181 or  GPO Box 2284, Melbourne
3001               .***.        ***  AIDS Pathogenesis Research
Unit    _ _|\
* | | | *        * | |  VHPF Flow Cytometry Program Group    /
\   *   * | | | *    * | | |  Sidney Myer Fund Flow Cytometry Unit
\_.--._/ * * | | |  *             O   *** ***  Monash Central School
of Medicine (AMREP)  Flow-Cytometry and Sorting Suite Co-ordinator
tel: (+61 3) 9282 2246 (Burnet) or 9903-0601 (Monash)  fax:(+61 3)
9282 2100  email: paukovic@burnet.edu.au   This attachment - 'Purdue
CD8B staining.doc' - 31.23 KBytes - can be viewed at
http://www.cyto.purdue.edu/MD-parts/009eeed9cf8a14282a5f6bfed895a218b7bd8b5f.doc
Received on Fri Mar 23 15:18:01 2007
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Despite of my positive experience with the FACSFlow I would not buy
it   again.

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From: <akos.szilvasi@novartis.com>
Date: Thu Mar 29 2007 - 14:20:34 EDT
Elizabeth,    I have used several of the FACSFlow systems for a long
time. We had no   problem with any of the units.
They are reliable and need practically no   maintenance.     Despite
of my positive experience with the FACSFlow I would not buy it
again.     There is a better solution for the LSR II. BD offered us 20
liters steel   tanks. This is the same tank as the regular LSRII tank
but much bigger.   Answering your other question, yes, the HTS uses a
lot of sheath fluid   because the sheath fluid serves as the wash and
rinse agent to the   sampler. This is very convenient for not having
to monitor yet an other   container's liquid level. Actually we
switched to distilled water for   simplicity and less complication
with salt deposits. In order to monitor   the water level in the tanks
we put them onto a bathroom scale. Once you   calibrate the scale
(read the weight of the empty tank and the full tanks)   you know when
to fill it up. We have not noticed any pressure instability   with low
level of water in the sheath tanks.    Akos
__________________________   Akos Szilvasi
NIBRI Core Laboratory Services  manager  USCA, 601-5301  Novartis
Institutes for BioMedical  Research, Inc.  100 Technology Square
Cambridge, MA 02139  USA  Phone: +1 617 8717177  Email :
akos.szilvasi@novartis.com              "Kraus, Elizabeth"
<elizabkr@BaylorHealth.edu>   03/29/2007 01:56 PM    To  Cytometry
Mailing List <cytometry@flowcyt.cyto.purdue.edu>  cc    Subject  LSR
II FACSFlow system                Hello all...    Please let me hear
from you if you have an LSR II with the FACSFlow and   HTS Systems.
My  questions are:    1) Would you purchase the FACSFlow System
again?  2) Does the HTS use more sheath than regular tubes?    For
those of you with the standard sheath system... How long does a tank
last if running  samples all day long?    Thanks in advance!
Elizabeth    Elizabeth T. Kraus  Manager, Flow Cytometry core
Facility  Baylor Institute for Immunology Research  3434 Live Oak,
Suite 200.1  Dallas,  TX.  75204  214-820-3586
elizabkr@bhcs.com      -----Original Message-----  From: Julie
Bertout [mailto:julie.bertout@ibl.fr]  Sent: Wednesday, March 28, 2007
8:58 AM  To: cyto-inbox  Subject: lymphocyte cell cycle analysis
Hello,    Does anybody have a protocol to study cell cycle of
lymphocytes purified  from blood?    I suggested the regular PI
staining I used for cell line  - fixation with cold EtOH 80%,  -
storage at 40C,  - stain 1 million cells with 100ug IP and 125ug RNase
30min @ 370C  but the morphology of the cells completely changed after
this treatment.  The lymphocyte were quiescent. The researcher did a
positive control  using PHA to induce cell cycle but the populations
changed even more...    Thank you for your advices.    Julie Bertout
cytometry lab  Institut Pasteur de Lille  1 rue du professeur
Calmette  59800 Lille  France    This e-mail, facsimile, or letter and
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Received on Fri Mar 30 12:18:00 2007
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________________________________________
buybroker@yahoo.com - 25 Dec 2007 20:38 GMT
> These data are already available on our website at Purdue are numbers
> for over 80 core labs in the country
[quoted text clipped - 1983 lines]
>  * | | | *        * | |  VHPF Flow Cytometry Program Group    /
> \   *   * | | | *    * | | |  Sidney Myer Fund Flow Cytometry UnitFrom: Mario Roederer <roede...@drmr.com>
Date: Mon May 28 2007 - 21:03:56 EDT

Re: LSR II vs. FACScanto

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From: Mario Roederer <roede...@drmr.com>
Date: Mon May 28 2007 - 21:03:56 EDT

Note that an 18 color machine should be purchased not because you
are
doing 18 colors, but because you are doing 10-12 colors!  We have
had
18-color capability for 4 years, but rarely do more than 16...

The point is that it is much easier to do a 12 color experiment on
an
18 color machine, than it is to do 12 colors on a 12 color machine
(because of the considerable additional flexibility of mixing and
matching reagents when you have additional colors available).

Nowadays, it is much more common to try to do 9-color experiments.
Designing 9-color panels on a 9-color machine is far more difficult
than it is on a 12, 15, or 18-color machine.  If you are planning on
doing 6-color experiments, then a 9-color machine is reasonable.

My point is only that the number of colors that your machine is
capable of should exceed, hopefully by a reasonable margin, the
number of colors that you wish to do...

mr

(PS, I think Cantos are capable of 8 colors nowadays... but why
would
you get a Canto, if you could have an LSR II?  Unless space is an
issue... and the LSR definitely requires more space.)

On May 25, 2007, at 1:51 PM, Randy T. Fischer wrote:

- Hide quoted text -
- Show quoted text -

> Hi Mike,

> Why are you limiting yourself to either an LSR II or a Canto?  You
> should look around as there are other options.  A great deal
[quoted text clipped - 6 lines]
> do at least 18 colors, but do you need or anticipate needing this
> capability?  The LSR II is also huge and requires a special bench.

> Randy T. Fischer, NIH/NIAMS
> B Cell Biology Group
[quoted text clipped - 3 lines]
> (301) 594-3537 (voice)
> (301) 402-2209 (fax)

> From: Mindy Hankrasky <mhankra...@hotmail.com>
> Date: Thu, 24 May 2007 13:02:28 -0700
> To: Cytometry Mailing List <cytome...@flowcyt.cyto.purdue.edu>
> Subject: LSR II vs. FACScanto

> Hi, All,

> I am considering to buy a FACS machine. But I have no knowledge
> about the comparison of LSR II and FACScanto. Could someone give me
> suggestion in terms of function and price?

> Thanks

> Mike

> Change is good. See what's different about Windows Live Hotmail.
> Check it out! <www.windowslive-hotmail.com/learnmore/default.html?
> locale=en-us&ocid=RMT_TAGLM_HMWL_reten_changegood_0507>

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Received on Tue May 29 11:38:00 2007

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***************************************************************************­
*********************************
Purdue Cytometry Mailing List: Re: DNA analysis software
... J. Herbert > Technical Support Manager > Verity Software
House ...
sender immediately by return e-mail, delete this e-mail and destroy
any copies. Any ...
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26 May 07
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Re: DNA analysis software

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From: Xiaoping Wu <xiaopi...@psbc.org>
Date: Thu May 24 2007 - 17:04:13 EDT

We have Modfit 3.0 and our computer is installed with both OS 9 and
OS
X. We did not find problem with printing.

On May 23, 2007, at 2:33 PM, Turbov, Jane wrote:

- Hide quoted text -
- Show quoted text -

> Hello Flow-ers,
> I would like to revisit the question of DNA analysis. We have ~ 12
[quoted text clipped - 4 lines]
> but printing may be an issue.
> http://www.vsh.com/Support/KBDetail.asp?incident=1132

> Our lab manager suggested FLOJO
> http://www.flowjo.com/specproliferation.html but this might be too
> sophisticated for our needs.

> Does anyone have any recommendations?

> Thanks,

> Jane Turbov
> Department of OBGYN Research
[quoted text clipped - 3 lines]
> TEL:  847-570-4021
> FAX: 847-733-5256

> From: VSH Tech Support [mailto:T...@vsh.com]
> Sent: Wednesday, February 21, 2007 10:30 AM
> To: Cytometry Mailing List
> Subject: RE: DNA analysis software

> Hello Ibtissam,

>  ModFit LT, for PC or Mac, has advanced modeling capability for
> research applications in DNA cell cycle analysis.  You may use any of
[quoted text clipped - 3 lines]
> complete cell cycle analysis on any number of sub-populations with a
> single click of a button.

>   For an overview, visit http://www.vsh.com/products%a0.

>  Best regards,

>  Don

>  Donald J. Herbert
>  Technical Support Manager
>  Verity Software House

>  ________________________________

>  From: Ibtissam Abdul-Jabbar [mailto:iajab...@cicr.uq.edu.au]
>  Sent: Wednesday, February 14, 2007 11:41 PM
>  To: cyto-inbox Subject: DNA analysis software

>  Dear All, before buying software to analyse DNA on PC, I would like
> to get your opinion. I already have ModFit for Macintosh.

>  What are you using and what do you recommend for research purposes.

>  Is MultiCycle Av the one of choice?

>  I appreciate your comments.

>  Ibtissam A Jabbar (PhD)

>   Manager of the FACS facilities

>   Diamantina Institute for Cancer, Immunology and Metabolic Medicine
> (DI)

>  The University of Queensland

>  Level 4 R Wing

> Princess Alexandra Hospital

> Ipswich Rd Buranda QLD 4102

> Australia

>  Ph: 07 3240 5945

>  Fax: 07 3240 5946

>  Mob: 0401154744

Xiaoping Wu, Ph.D.
Flow Cytometry Laboratory
Research Division of the Puget Sound Blood Center
1201 Ninth Avenue
Seattle, Washington 98101

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Purdue Cytometry Mailing List: DNA analysis software
... a conversion at no charge. I invite you to contact Verity
Software
House directly with any specific questions or issues. Best
regards ...
issues. Best regards, Don Donald J. Herbert Technical Support Manager
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26 May 07
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Re: DNA analysis software

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From: Xiaoping Wu <xiaopi...@psbc.org>
Date: Thu May 24 2007 - 17:04:13 EDT

We have Modfit 3.0 and our computer is installed with both OS 9 and
OS
X. We did not find problem with printing.

On May 23, 2007, at 2:33 PM, Turbov, Jane wrote:

- Hide quoted text -
- Show quoted text -

> Hello Flow-ers,
> I would like to revisit the question of DNA analysis. We have ~ 12
[quoted text clipped - 4 lines]
> but printing may be an issue.
> http://www.vsh.com/Support/KBDetail.asp?incident=1132

> Our lab manager suggested FLOJO
> http://www.flowjo.com/specproliferation.html but this might be too
> sophisticated for our needs.

> Does anyone have any recommendations?

> Thanks,

> Jane Turbov
> Department of OBGYN Research
[quoted text clipped - 3 lines]
> TEL:  847-570-4021
> FAX: 847-733-5256

> From: VSH Tech Support [mailto:T...@vsh.com]
> Sent: Wednesday, February 21, 2007 10:30 AM
> To: Cytometry Mailing List
> Subject: RE: DNA analysis software

> Hello Ibtissam,

>  ModFit LT, for PC or Mac, has advanced modeling capability for
> research applications in DNA cell cycle analysis.  You may use any of
[quoted text clipped - 3 lines]
> complete cell cycle analysis on any number of sub-populations with a
> single click of a button.

>   For an overview, visit http://www.vsh.com/products%a0.

>  Best regards,

>  Don

>  Donald J. Herbert
>  Technical Support Manager
>  Verity Software House

>  ________________________________

>  From: Ibtissam Abdul-Jabbar [mailto:iajab...@cicr.uq.edu.au]
>  Sent: Wednesday, February 14, 2007 11:41 PM
>  To: cyto-inbox Subject: DNA analysis software

>  Dear All, before buying software to analyse DNA on PC, I would like
> to get your opinion. I already have ModFit for Macintosh.

>  What are you using and what do you recommend for research purposes.

>  Is MultiCycle Av the one of choice?

>  I appreciate your comments.

>  Ibtissam A Jabbar (PhD)

>   Manager of the FACS facilities

>   Diamantina Institute for Cancer, Immunology and Metabolic Medicine
> (DI)

>  The University of Queensland

>  Level 4 R Wing

> Princess Alexandra Hospital

> Ipswich Rd Buranda QLD 4102

> Australia

>  Ph: 07 3240 5945

>  Fax: 07 3240 5946

>  Mob: 0401154744

Xiaoping Wu, Ph.D.
Flow Cytometry Laboratory
Research Division of the Puget Sound Blood Center
1201 Ninth Avenue
Seattle, Washington 98101

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Received on Fri May 25 13:38:00 2007

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