Purdue Cytometry Mail List CALL FOR ***ACTION***DEMAND MORE MONEY FROM **NIH** GRANTS**CALL YOU CONGRESS WHEN THEY RETURN TO WASHINGTON AND TELL THEM THIS......THEN REPORT BACK.****REMIND YOUR REPRENTATIVE OF THE IMPROTANTANCE....HERE IS WHAT YOU SHOULD TELL THEM if you don't know what ot ask for?***We are calling upon you again during this Independence Day holiday!****DO YOU UNDERSTAND! A 1.9% INCREASE IS NEEDED! DO NOT FORGET..YOUR 2008 CONGRESS BUDGET! ISAC CONGRESS 2008 MUST KNOW FOR NIH 2008 RALLY PEOPLE .

Purdue Cytometry Mail List CALL FOR ACTIONDEMAND MORE MONEY FROM NIH GRANTSCALL YOU CONGRESS WHEN THEY RETURN TO WASHINGTON AND TELL THEM THIS......THEN REPORT BACK.REMIND YOUR REPRENTATIVE OF THE IMPROTANTANCE....HERE IS WHAT YOU SHOULD TELL THEM if you don't know what ot ask for?*We are calling upon you again during this Independence Day holiday!****DO YOU UNDERSTAND! A 1.9% INCREASE IS NEEDED! DO NOT FORGET..YOUR 2008 CONGRESS BUDGET! ISAC CONGRESS 2008 MUST KNOW FOR NIH 2008 RALLY PEOPLE .

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Mitch Haynes - 13 Dec 2007 20:19 GMT

June 29, 2007
NIH Funding
Another thread from Purdue worth posting:

We are calling upon you again during this Independence Day holiday
period to ask you to contact your Representative in person at a local
event or by telephone to the local office by July 9th. When Congress
returns to Washington after the 4th of July recess, the House of
Representatives will consider a 1.9% increase to the NIH budget.
Between now and then, you have the opportunity to remind your
Representative of the importance of your work and let them know about
any difficulties you have encountered because of shrinking budgets.

Members of Congress have told us that they are not hearing from
researchers in the community. We must turn this around. Members and
their staffs are receptive to hearing from researchers about how the
proposed FY 2008 budget will affect their research and their careers.

Please contact your Representative either in person at a local
Independence Day event or by telephone before July 9th. Tell them
your
story and let them know that they must make cancer and biomedical
research funding a national priority.

In your contact with your Representative, please mention any of the
research challenges you are facing at this time, for example: -
denials
or delays in getting grant funding for meritorious research
-the impact of cuts to grants already funded
-staffing and equipment shortages
-the loss of bright young investigators to other fields
-reduced opportunities for patients to get on therapeutic clinical
trials
-cancellation or halting of important clinical trials.

In short, your Representative needs to hear about the serious effect
these low budgets are having on your research progress. Urge them to
make cancer research funding a national priority. A few minutes of
your time could help get NIH and NCI funding back on track.

Thank you for responding to this request and have an enjoyable
holiday.

William N. Hait, M.D., Ph.D., President
William G. Nelson V, M.D., Ph.D., Chair, Science Policy & Legislative
Affairs Committee
Margaret Foti, Ph.D., M.D. (h.c.), Chief Executive Officer

P.S. Please let AACR know you have made a contact with your
Representative by sending us a quick e-mail at legaffa...@aacr.org.
Just write: "I have contacted Representative X in person/by phone."

Reply to this Message

Mitch Haynes - 15 Dec 2007 15:31 GMT

> June 29, 2007
> NIH Funding
[quoted text clipped - 47 lines]
> Representative by sending us a quick e-mail at legaffa...@aacr.org.
> Just write: "I have contacted RepresentaISAC Homepage - Members of the ISAC Committees (2007)

Phone: +90 212 320 64 00. J. Paul Robinson (ex-officio) Term:
2006-2008 .... FLOW CYTOMETRY ISSUES. Jonni Moore Chair Term:
2006-2008 ...
www.isac-net.org/content/view/572/126/ - 35k - Cached - Similar pages
- Note this

ISAC Homepage - ISAC E-News -- June, 2007 -
Best wishes. J. Paul Robinson, President ... ISAC 2008 Congress
Organizing Committee: Bob Murphy (Chair), Derek Davies, Lori Krueger,
John Nolan, ...
www.isac-net.org/content/view/593/119/ - 53k - Cached - Similar pages
- Note this

ISAC E-News
File Format: PDF/Adobe Acrobat - View as HTML
J. Paul Robinson, President ... Report from the 2008 Congress
Organizing Committee ..... introduced possibilities of integrating
these with FlowJo. ...
www.zangani.com/files/2007-0608-ISAC_E-News_June2007.pdf - Similar
pages - Note this

ISAC Homepage - Members of the ISAC Committees (2007)
J. Paul Robinson (ex-officio) Term: START AS EDITOR-TERMINATION AS
EDITOR .... FLOW CYTOMETRY ISSUES. Jonni Moore Chair Term:
2006-2008 ...
www.isac-net.org/content/view/572/126/ - 35k - Cached - Similar pages
- Note this

Cytometry Software
11color.hqx, BinHex file containing a StuffIt archive with FlowJo, its
workspace file, ... J. Paul Robinson, Ph.D., Director PUCL, Hansen
Hall, B050, ...
www.cyto.purdue.edu/flowcyt/software/Flowjo.htm - 10k - Cached -
Similar pages - Note this

Cytometry Software -
11color.hqx, BinHex file containing a StuffIt archive with FlowJo, its
workspace file, ... J. Paul Robinson, Ph.D., Director PUCL, Hansen
Hall, B050, ...
www.cyto.purdue.edu/flowcyt/software/Flowjo.htm - 10k - Cached -
Similar pages - Note this
[ More results from www.cyto.purdue.edu ]

The Daily Dongle
Rant about FlowJo's goings-on. ... J Paul Robinson delivered an
effective summary of the problem, and updates on efforts to combat it,
starting with a ...
flowjo.typepad.com/ - 40k - Cached - Similar pages - Note this

PDF]
GETTING STARTED
File Format: PDF/Adobe Acrobat - View as HTML
FlowJo analyzes flow cytometry data, regardless of which FACS
machine ..... J. Paul Robinson and Harry A. Crissman. Methods in Cell.
Biology: Flow Cytometry ...
66.241.70.127/bin/gettingstartedweb.pdf - Similar pages - Note this

School of Medicine: Research: Core Facilities: Flow Cytometry Core ...
Instructions. How to use FlowJo off-campus using an Emory VPN
connection: ... 4. Handbook of Flow Cytometry Methods. Edited by J
Paul Robinson. ...
www.corelabs.emory.edu/fcm/instructions.htm - 12k - Cached - Similar
pages - Note this

Useful Links & Resources - Flow Cytometry Site
General. Purdue University Cytometry Labs (J. Paul Robinson) ...
Software. FlowJo. http://flowjo.com/. FACSPress and FACSAssistant.
http://www.fcspress.com/ ...
cbr.med.harvard.edu/content/facilities/cytometry/website/pages/
links.html - 42k -

Pursue your dreams, no matter what: [RAAM '07 - Patrick Autissier ...
Treestar, the company that created FlowJo, one of the most popular
scientific analysis ... Paul Robinson. - -. J. Paul Robinson. SVM
Professor of Cytomics ...
patrickautissier.blogspot.com/2007/05/raam-07-patrick-autissier-
newsletter-05.html - 71k -

Meeting 2007
J Paul Robinson (Purdue University, USA). Multicolour Flow
Cytometry ... Cytek Development, Distrilab, FlowJo, Guava
Technologies, Invitrogen, ...
www.flowcytometryuk.org/fliers/Annual1.html - 8k - Cached - Similar
pages - Note this

PDF]
Tree Star
File Format: PDF/Adobe Acrobat - View as HTML
J. Paul Robinson,. 4. 1. Laboratory of Confocal Microscopy and Image
Analysis, ... Edward Podniesinski and Dr. Paul Wallace, Laboratory of
Flow ...
gliifca.org/pdf/GLIIFCA-2004.pdf - Similar pages - Note this

Purdue Cytometry Mailing List: By Date
J. Paul Robinson (Thu Jun 21 2007 - 21:44:28 EDT) ... FlowJo Technical
Support - Maciej Simm (Thu Jan 18 2007 - 18:41:19 EST); Reed, Doug S
Dr USAMRIID (Wed ...
www.cyto.purdue.edu/hmarchiv/Current/subject.htm - 261k - Cached -
Similar pages - Note this

Purdue Cytometry Mailing List: By Date
Petra Hildebrandt (Mon Oct 15 2007 - 05:55:47 EDT); Update info on
using DIVA exported fiels in FlowJo Houston, Jim (Fri Oct 12 2007 -
14:18:23 EDT) ...
www.cyto.purdue.edu/hmarchiv/Current/thread.htm - 293k - Cached -
Similar pages - Note this
[ More results from www.cyto.purdue.edu ]
tive X in person/by phone."

***************************************************************************************************************

Reply to this Message

Mitch Haynes - 22 Dec 2007 18:30 GMT

> June 29, 2007
> NIH Funding
[quoted text clipped - 47 lines]
> Representative by sending us a quick e-mail at legaffa...@aacr.org.
> Just write: "I have contacted Representative X in person/by phone."

PURDUE CYTOMETRY MAIL LIST SOFTWARE SHOULD BE BIG TOPIC
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> Co-Chairs: Bartek Rajwa and J Paul Robinson. The Cytometry of Flow Jo ISAC 2008 New Flow Cytomety Mail List Rules and Sales Regulations NOT IN THE NEWS BECAUSE THEY DO NOT WANT YOU TO KNOW Options

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Mitch Haynes View profile
More options Dec 22, 6:57 am

Newsgroups: misc.health.aids
From: Mitch Haynes <mitchhay...@gmail.com>
Date: Sat, 22 Dec 2007 04:57:11 -0800 (PST)
Local: Sat, Dec 22 2007 6:57 am
Subject: > Co-Chairs: Bartek Rajwa and J Paul Robinson. The Cytometry
of Flow Jo ISAC 2008 New Flow Cytomety Mail List Rules and Sales
Regulations NOT IN THE NEWS BECAUSE THEY DO NOT WANT YOU TO KNOW
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http://flowjo.typepad.com/the_daily_dongle/2007/06/index.html

June 29, 2007
NIH Funding

Another thread from Purdue worth posting:

We are calling upon you again during this Independence Day
holiday
period to ask you to contact your Representative in person at a local
event or by telephone to the local office by July 9th. When Congress
returns to Washington after the 4th of July recess, the House of
Representatives will consider a 1.9% increase to the NIH budget.
Between now and then, you have the opportunity to remind your
Representative of the importance of your work and let them know about
any difficulties you have encountered because of shrinking budgets.

Members of Congress have told us that they are not hearing from
researchers in the community. We must turn this around. Members and
their staffs are receptive to hearing from researchers about how the
proposed FY 2008 budget will affect their research and their careers.

Please contact your Representative either in person at a local
Independence Day event or by telephone before July 9th. Tell them
your
story and let them know that they must make cancer and biomedical
research funding a national priority.

In your contact with your Representative, please mention any of
the research challenges you are facing at this time, for example: -
denials or delays in getting grant funding for meritorious research
-the impact of cuts to grants already funded
-staffing and equipment shortages
-the loss of bright young investigators to other fields
-reduced opportunities for patients to get on therapeutic
clinical
trials
-cancellation or halting of important clinical trials.

In short, your Representative needs to hear about the serious
effect these low budgets are having on your research progress. Urge
them to make cancer research funding a national priority. A few
minutes of your time could help get NIH and NCI funding back on
track.

Thank you for responding to this request and have an enjoyable
holiday.

William N. Hait, M.D., Ph.D., President
William G. Nelson V, M.D., Ph.D., Chair, Science Policy &
Legislative Affairs Committee
Margaret Foti, Ph.D., M.D. (h.c.), Chief Executive Officer

P.S. Please let AACR know you have made a contact with your
Representative by sending us a quick e-mail at legaffa...@aacr.org.
Just write: "I have contacted Representative X in person/by phone."

June 29, 2007 in Current Affairs | Permalink | Comments (0)
June 27, 2007
http://flowjo.typepad.com/the_daily_dongle/2007/06/index.html
***************************************************************************
*********************************************
Re: mr on Apple web site
* This message: [ Message body ] [ More options ]
* Related messages: [ Next message ] [ Previous message ] [ In
reply
to ] [ Next in thread ] [ Replies ]
From: Howard Shapiro <h...@shapirolab.com>
Date: Wed Aug 25 2004 - 18:15:38 EST

Robin Barclay wrote:

>Well .... some of us PC afficianados hate Macs just as much - personally I
>have hated them since they first made it difficult to access their DOS and
[quoted text clipped - 9 lines]
>for interfacing with lab equipment..... and they can "look cool" if you shop
>around for the right case if that's important to you.

I can't pass up the opportunity to get into the PC/Mac battle - I
hate
them
both, but there aren't really alternatives (yes, I know, there's
Linux, but
it's probably even harder to hook up a Linux system to hardware than
it is
to hook a Mac up to hardware).

Of course, what started this thread was the assertion in the piece
about
Mario on Apple's web site that there were 15,000 to 20,000 Mac-based
flow
cytometers out there. The best estimates I had from industry people
in
early 2003 when I was finishing up the 4th Edition of PFC was that
the
total number of systems from all manufacturers in use was under
20,000. If
that's correct, BD would need a 75% market share to account for the
low end
estimate of 15,000 Mac-based machines. I'd be interested to know
where
the
15,000 to 20,000 figure came from.

The old (68000 series and possibly early PowerPC) Macs were
difficult,
but
not impossible to connect to hardware; it was easier to work with the
PC's
ISA bus, which, while slow, was perfectly adequate to do most flow
cytometric data acquisition and analysis. There were decent versions
of
Forth, which was one of the first and best languages designed for
controlling hardware from mini- and microcomputers, for both PC (DOS)
and
the Mac (Forth was the first Mac programming language made
commercially
available, at a time when the only other option was buying the very
expensive Lisa from Apple on which to develop Mac software). I found
MacForth easier to program with than the Windows Forths (or other
Windows
languages, e.g., Delphi), but my old Macs used to crash all the time.

So what makes me unhappy with both Windows [and Windows machines] and
the
Mac in their current incarnations? It is now significantly harder for
mere
mortals to write software to get hardware to communicate with either
PCs or
Macs; the gain in complexity associated with the PCI bus, USB/USB2,
FireWire(IEEE 1394), etc. is greater than the gain in speed and
convenience. Also, in making the operating systems more stable (and
Win XP,
despite its security issues, is almost as stable as Mac's Unix-based
OS X),
both Microsoft and Apple elected to eliminate the ability of their
computer
hardware to respond rapidly to interrupts (latencies are now in the
tens of
thousands of instruction cycles), meaning that any really fast
hardware
attachment for either a PC or a Mac now needs to have a DSP in it,
whereas
if the fast interrupt response had been preserved, the hardware
attachments
could have been made much simpler and cheaper. Linux also takes fast
interrupt response off the table, so it doesn't represent a viable
alternative. If you go to Apple's web site and look at what data
acquisition hardware is available for Macs, particularly for Macs
running
OS X, there isn't much, and many of the companies that supported the
Mac in
that area have dropped Mac support for their newer products. That is
undoubtedly one big reason why BD's newer digital pulse processing
cytometers are running on the PC platform.

That doesn't stop anybody from analyzing FCS files on Macs. FlowJo is
well-conceived flow cytometry software; one reason it is as good as
it
is
is that it was written by people who do a lot of flow cytometry, and
cutting edge flow cytometry at that. But there are other people who
do
a
lot of good flow cytometry who have written good software, for PCs as
well
as for Macs.

For the record, I have a G4 PowerBook, which I use mostly for the
iLife
applications, which are slick. It doesn't crash more than once every
couple
of months, but the same is true of my Windows 2000 and XP systems. OS
X can
be as infuriating as Windows when one or another aspect of it goes
counter
to your intuition or to what you have gotten used to. Macs, while
somewhat
more expensive, are much better made than many PCs, and they are
certainly
aesthetically pleasing. If there were a reasonable alternative to
Microsoft's Office applications for the Mac, I might consider
switching.
The 12.1" PowerBook is a nice portable, but it's over a pound heavier
than
the Fujitsu laptop I now use, which has pretty much the same speed,
memory,
and drive capabilities (OK, not DVD-R, but I don't burn a lot of
DVDs).

However, I really wish Apple had stuck with the plan they had a few
years
back of writing a Mac operating system for Intel hardware. I think
that
died when Microsoft bailed Apple out with a few hundred mil. If it
were
possible to run OS X and XP mano a mano on the same hardware, there'd
be a
more rational basis for comparison. But, as may be the case for the
November election, minds, once made up, are not easily changed.

-Howard
Received on Thu Aug 26 16:38:00 2004
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Re: mr on Apple web site
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From: Howard Shapiro <h...@shapirolab.com
<mailto:h...@shapirolab.com?
subject=Re:%20mr%20on%20Apple%20web%20site>>
Date: Wed Aug 25 2004 - 18:15:38 EST

Robin Barclay wrote:

I can't pass up the opportunity to get into the PC/Mac battle - I
hate
them
both, but there aren't really alternatives (yes, I know, there's
Linux, but
it's probably even harder to hook up a Linux system to hardware than
it is
to hook a Mac up to hardware).

Of course, what started this thread was the assertion in the piece
about
Mario on Apple's web site that there were 15,000 to 20,000 Mac-based
flow
cytometers out there. The best estimates I had from industry people
in
early 2003 when I was finishing up the 4th Edition of PFC was that
the
total number of systems from all manufacturers in use was under
20,000. If
that's correct, BD would need a 75% market share to account for the
low end
estimate of 15,000 Mac-based machines. I'd be interested to know
where
the
15,000 to 20,000 figure came from.

The old (68000 series and possibly early PowerPC) Macs were
difficult,
but
not impossible to connect to hardware; it was easier to work with the
PC's
ISA bus, which, while slow, was perfectly adequate to do most flow
cytometric data acquisition and analysis. There were decent versions
of
Forth, which was one of the first and best languages designed for
controlling hardware from mini- and microcomputers, for both PC (DOS)
and
the Mac (Forth was the first Mac programming language made
commercially
available, at a time when the only other option was buying the very
expensive Lisa from Apple on which to develop Mac software). I found
MacForth easier to program with than the Windows Forths (or other
Windows
languages, e.g., Delphi), but my old Macs used to crash all the time.

So what makes me unhappy with both Windows [and Windows machines] and
the
Mac in their current incarnations? It is now significantly harder for
mere
mortals to write software to get hardware to communicate with either
PCs or
Macs; the gain in complexity associated with the PCI bus, USB/USB2,
FireWire(IEEE 1394), etc. is greater than the gain in speed and
convenience. Also, in making the operating systems more stable (and
Win XP,
despite its security issues, is almost as stable as Mac's Unix-based
OS X),
both Microsoft and Apple elected to eliminate the ability of their
computer
hardware to respond rapidly to interrupts (latencies are now in the
tens of
thousands of instruction cycles), meaning that any really fast
hardware
attachment for either a PC or a Mac now needs to have a DSP in it,
whereas
if the fast interrupt response had been preserved, the hardware
attachments
could have been made much simpler and cheaper. Linux also takes fast
interrupt response off the table, so it doesn't represent a viable
alternative. If you go to Apple's web site and look at what data
acquisition hardware is available for Macs, particularly for Macs
running
OS X, there isn't much, and many of the companies that supported the
Mac in
that area have dropped Mac support for their newer products. That is
undoubtedly one big reason why BD's newer digital pulse processing
cytometers are running on the PC platform.

That doesn't stop anybody from analyzing FCS files on Macs. FlowJo is
well-conceived flow cytometry software; one reason it is as good as
it
is
is that it was written by people who do a lot of flow cytometry, and
cutting edge flow cytometry at that. But there are other people who
do
a
lot of good flow cytometry who have written good software, for PCs as
well
as for Macs.

For the record, I have a G4 PowerBook, which I use mostly for the
iLife
applications, which are slick. It doesn't crash more than once every
couple
of months, but the same is true of my Windows 2000 and XP systems. OS
X can
be as infuriating as Windows when one or another aspect of it goes
counter
to your intuition or to what you have gotten used to. Macs, while
somewhat
more expensive, are much better made than many PCs, and they are
certainly
aesthetically pleasing. If there were a reasonable alternative to
Microsoft's Office applications for the Mac, I might consider
switching.
The 12.1" PowerBook is a nice portable, but it's over a pound heavier
than
the Fujitsu laptop I now use, which has pretty much the same speed,
memory,
and drive capabilities (OK, not DVD-R, but I don't burn a lot of
DVDs).

However, I really wish Apple had stuck with the plan they had a few
years
back of writing a Mac operating system for Intel hardware. I think
that
died when Microsoft bailed Apple out with a few hundred mil. If it
were
possible to run OS X and XP mano a mano on the same hardware, there'd
be a
more rational basis for comparison. But, as may be the case for the
November election, minds, once made up, are not easily changed.

-Howard
Received on Thu Aug 26 16:38:00 2004
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RE: mr on Apple web site
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From: Fischer, Randy (NIH/NIAMS) <fisch...@mail.nih.gov>
Date: Mon Aug 23 2004 - 16:18:55 EST
Paul,

I know that for a long time, anyone who used a BD flow cytometer had
little
choice but to use a MAC-data acquisition required it. And, one of
the
best
third party software packages was FlowJo which only ran on a MAC.
Mario,
being a FlowJock, naturally was heavily exposed to this atmosphere.
However, most of our other laboratory instrumentation appears to run
on the
IBM clone type of PC-ELISA reader, luminometer, ELISpot reader, and
AFFYMETRIX machine to name the first ones that come to mind. Of
course,
because Flow is such a key component and FlowJo works so well, we
have
MACs
(and associated cinema displays) at all of our desks. So, Mario is
probably
pretty close as I guess we now run about 50% MAC, and for most things
they
do run better than our PCs for similar applications, but mostly the
two
platforms tend to be used for very disparate applications and we need
both.

Wish more science companies would move to MAC, but as Microsoft
probably
owns a minority share in most companies, that will not likely happen
in our
lifetimes.

Haven't read the article yet, but hopefully Mario remembered to cite
you for
all the work on the Flow website/mailing list.

Randy T. Fischer
NIH/NIAMS
Building 10, Room 6D50
9000 Rockville Pike
Bethesda, MD 20892
(301) 594-3537
fisch...@mail.nih.gov

- Hide quoted text -
- Show quoted text -

> ----------
> From: J. Paul Robinson
> Reply To: j...@flowcyt.cyto.purdue.edu
> Sent: Friday, August 20, 2004 2:25 PM
> To: Cytometry Mailing List
> Subject: Re: mr on Apple web site

> ummm....Mario says..
> "In life sciences - particularly in research life sciences -
[quoted text clipped - 3 lines]
> have put on my helmet and armor....waiting...)
> paul

> > For all of the mr groupies out there in cytometry cyberspace. Don't wet
> your
> > pocket protectors over this.

> > Honestly though, well deserved praise for Mario & the Tree Star group:
> > http://www.apple.com/science/profiles/roederer
> > > _______________________
> > > Calman Prussin
> > > Laboratory of Allergic Diseases
> > > NIAID/ National Institutes of Health

> > The information in this e-mail and any of its attachments is
> confidential
[quoted text clipped - 10 lines]
> > and not expressly made on behalf of the NIAID by one of its
> representatives.

> J.Paul Robinson, PhD PH:(765)4940757
> Professor of Immunopharmacology
> Professor of Biomedical Engineering
> Purdue University FAX:(765)4940517
> EMAIL:j...@flowcyt.cyto.purdue.edu
> WEB: http://www.cyto.purdue.edu

> Have you seen our new HCS webpage?
> http://www.cyto.purdue.edu/hcs

Received on Tue Aug 24 16:18:00 2004
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***************************************************************************
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J PAUL ROBINSON

DON'T WET YOUR POCKET PROTECTORS

Re: mr on Apple web site
* This message: [ Message body ] [ More options ]
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From: J. Paul Robinson <j...@flowcyt.cyto.purdue.edu>
Date: Mon Aug 23 2004 - 18:46:12 EST
I knew that I should not have taken Mario's bait....but its
been a long summer.....!
paul

- Hide quoted text -
- Show quoted text -

> Paul:

> I use Macs exclusively. Between home and labs, I have 7.
> I hate Windows (I tell everyone, I don't do Windows).
[quoted text clipped - 5 lines]
> flat-panel display for my flow.
> Beverly

> Beverly E. Barton, Ph.D.
> Assistant Professor
> Department of Surgery/Division of Urology
> UMDNJ-NJMS MSB G519
> 185 S. Orange Avenue
> Newark, New Jersey 07103

> Telephone 973-972-0662
> E-mail barto...@umdnj.edu
> Telefacsimile 973-972-3892

> On Aug 20, 2004, at 2:25 PM, J. Paul Robinson wrote:

> > ummm....Mario says..
> > "In life sciences -- particularly in research life sciences --
[quoted text clipped - 3 lines]
> > have put on my helmet and armor....waiting...)
> > paul

> >> For all of the mr groupies out there in cytometry cyberspace. Don't
> >> wet your
> >> pocket protectors over this.

> >> Honestly though, well deserved praise for Mario & the Tree Star group:
> >> http://www.apple.com/science/profiles/roederer
> >>> _______________________
> >>> Calman Prussin
> >>> Laboratory of Allergic Diseases
> >>> NIAID/ National Institutes of Health

> >> The information in this e-mail and any of its attachments is
> >> confidential
[quoted text clipped - 10 lines]
> >> and not expressly made on behalf of the NIAID by one of its
> >> representatives.

> > J.Paul Robinson, PhD PH:(765)4940757
> > Professor of Immunopharmacology
> > Professor of Biomedical Engineering
> > Purdue University FAX:(765)4940517
> > EMAIL:j...@flowcyt.cyto.purdue.edu
> > WEB: http://www.cyto.purdue.edu

> > Have you seen our new HCS webpage?
> > http://www.cyto.purdue.edu/hcs

J.Paul Robinson, PhD PH:(765)4940757
Professor of Immunopharmacology
Professor of Biomedical Engineering
Purdue University FAX:(765)4940517
EMAIL:j...@flowcyt.cyto.purdue.edu
WEB: http://www.cyto.purdue.edu

Have you seen our new HCS webpage?
http://www.cyto.purdue.edu/hcs
Received on Tue Aug 24 15:58:00 2004
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Re: Web site changes
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From: Mario Roederer <roede...@drmr.com>
Date: Sat Sep 24 2005 - 15:05:50 EST
and undoubtedly saves me additional spam. I much prefer the
additional privacy.

mr

At 1:10 PM -0700 9/22/05, FloCyte Associates, INC wrote:

- Hide quoted text -
- Show quoted text -

>Please .... PLEASE may we have the old search capabilities for the
>membership list back! We can not search the new site for names in
[quoted text clipped - 4 lines]
>Thanks
>Sue

>At 05:09 PM 9/21/2005, J. Paul Robinson wrote:

>>Colleagues:
>>Just to bring you up to date on some new thngs:

>>Hope you have enjoyed the new ISAC website!! - Dr. Bartek
>>Rajwa (webmas...@isac-net.org) is the new editor - Greg
[quoted text clipped - 4 lines]
>>steadily but surely. We do listen to your needs as members
>>( ...tvs message received!).

>>The address is
>> http://www.isac-net.org
[quoted text clipped - 4 lines]
>>links to current science news, public policy, science
>>business. Company press releases are posted daily.

>>Remember Howard Shaprio's great cytometry text??
>>You can get access to Howard's entire book by going to:
>> http://tinyurl.com/ab274
>>which is a direct access to Invitrogens registration page
>>(your name and email are required).

>>Finally, the ISAC congress 2006 - you will be hearing about
>>abstract submission soon - I will let you into an unpublished
[quoted text clipped - 5 lines]
>>abstract submission deadlines soon.
>> http://www.isac2006.com

>>For companies interested in participating in the congress-
>>information has already been posted at the same address.

>>Oh - it seems that there are about 60% of this list are not
>>ISAC members!! Here's the deal - Join ISAC and save
>>yourself $160 for congress registration......and remember,
>>all this good stuff you get for nothing on these websites is
>>driven by ISAC members - please consider joining ISAC
>>and participating in the meeting.

>>Best wishes
>>J. Paul Robinson
>>President-elect of ISAC
>>Professor, Purdue University

>>J.Paul Robinson, PhD PH:(765)4940757
>>Professor of Immunopharmacology
>>Professor of Biomedical Engineering
>>Purdue University FAX:(765)4940517
>>EMAIL:j...@flowcyt.cyto.purdue.edu
>>WEB: http://www.cyto.purdue.edu

>>Have you seen our new HCS webpage?
>> http://www.cyto.purdue.edu/hcs

> Sue

>Susan DeMaggio, MS BSMT(ASCP) QCym
>President and CEO

>FloCyte Associates, INC.
>Flow Cytometry Recruiting and Training
>930 Golden Springs Dr. B
>Diamond Bar, CA 91765-4247

>909-860-7098
>FAX 860-7089

>FloC...@FloCyte.com
>www.FloCyte.com

>The biggest mistake that you can make is to believe that you are
>working for somebody else. Job security is gone. The driving force
>of a career must come from the individual. Remember: Jobs are owned
>by the company, you own your career! - Earl Nightingale, author,
>speaker, motivator

Received on Mon Sep 26 12:58:00 2005
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***************************************************************************
***************************************************
***************************************ONLY 3000 PEOPLE ON PURDUE
CYTOMETRY MAIL LIST******************
***************************************************************************
******************************************************
***************************************************************************
*******************************************************
**********************Racketeer Influenced and Corrupt Organizations
Act - *************http://en.wikipedia.org/wiki/
Racketeer_Influenced_and_Corrupt_Organizations_Act

***************************************************************************
*************************************************
WHY DID HE CLIAM KANECKI ASSOCIATES ARE A SCAM??????? JUST WANTED TO
TALK SOFTWARE!

***************************************************************************
*********************************************
5. You should ban this address, and basically indicate to your
members

that this is a scam.
***************************************************************************
**************************************************
While they claim to be legitimate, they are
acting

exactly as any scam artist...I already told them that they were
acting

as scammers and they got upset with me...well, if they don't desist,

they will find out how much influence we actually have...

***************************************************************************
****************************************************
they will find out how much influence we actually have...

they will find out how much influence we actually have...

they will find out how much influence we actually have...

***************************************************************************
*****************************************************
Dear Robert Murphy

NEW
FLOWCYTOMETRY SOFTWARE FOR ALL BD and Beckman/Coulter Flow Cytometers
CAN

PROCESS 24,000-42,000 SAMPLES PER HOUR.

FLOW CYTOMETRY FCS CYTO PRO QUICK FACS

http://www.kanecki.com/flow.html
***************************************************************************
******************************************************

[PDF]
GREAT LAKES INTERNATIONAL IMAGING AND FLOW CYTOMETRY
ASSOCIATION*********
File Format: PDF/Adobe Acr

obat - View as HTML
J. Paul Robinson, Philip Marder. Iowa. Bruce Pesch. Michigan ....
flow
cytometry software. Clearly, traditional cytometers can not handle
the
demands of HTS ...
gliifca.org/pdf/GLIIFCA-2001.pdf - Similar pages - Note this

DON'T FOR GET RAJWA BARTEK********

[PDF]
International Congress Montpellier 2004
File Format: PDF/Adobe Acrobat - View as HTML
Co-Chairs: Bartek Rajwa and J Paul Robinson. The Cytometry of
Plants .... analytical cytometry publishing and software companies,
pharmaceuti- ...
www.afc.asso.fr/actu/congres/isac04.pdf - Similar pages - Note this
***************************************************************************
*******************************************************
***************************************************************************
******************************************************
**********************************************PRESIDENT OF ISAC J
PAUL
ROBINSON*********************************
***********************************HEAD OF PURDUE
CYTOMETRY****************************************************
***********JUST ABOUT EVERY MAIL LINK IN THE WORD AND*** NON PROFIT
CYTOMETRY ORG. ???*******
***************************************************************************
***********************************************
Cytometry SoftwareIn some cases (e.g. MFI), "free" software is free
only for non-profit use; for-profit ... J. Paul Robinson, Ph.D.,
Director PUCL, Hansen Hall, B050, Purdue ...
www.cyto.purdue.edu/flowcyt/software/proscons.htm - 15k - Cached -
Similar pages - Note this

GREAT WAY TO GET IN THE DOOR WITH NON PROFIT.....

***************************************************************************
***************************************************
Personalized Results 1 - 10 of about 5,820 for J PAUL ROBINSON
CYTOMETRY BOOKS. (0.36 seconds)
Personalized Results 1 - 10 of about 5,820 for J PAUL ROBINSON
CYTOMETRY BOOKS. (0.36 seconds)
Personalized Results 1 - 10 of about 5,820 for J PAUL ROBINSON
CYTOMETRY BOOKS. (0.36 seconds)
Personalized Results 1 - 10 of about 5,820 for J PAUL ROBINSON
CYTOMETRY BOOKS. (0.36 seconds)

Joseph Robinson: ZoomInfo Business People Information*********
... Paul Robinson, chair of the Optics East Life Sciences symposium
(see sidebar) and director of Purdue University Cytometry
Laboratories
(West Lafayette, ...
www.zoominfo.com/people/Robinson_J._-82453.aspx - 24k -

Joseph Robinson: ZoomInfo Business People Information
... Paul Robinson, chair of the Optics East Life Sciences symposium
(see sidebar) and director of Purdue University Cytometry
Laboratories
(West Lafayette, ...
www.zoominfo.com/people/R

obinson_J._-82453.aspx - 24k -

Purdue Cytometry Mailing List: RE: CellQuest data files
Eric Martz, Professor of Immunology ema...@microbio.umass.edu Dept
Microbiology Voice: 413-545-2325 FAX: 413-545-1578 Morrill IVN 203,
Box 35720, ...
www.cyto.purdue.edu/hmarchiv/1995/1776.htm - 8k - Cached - Similar
pages - Note this
[ More results from www.cyto.purdue.edu ]

UMass/Amherst Flow Cytometry Facility - 4 visits - Nov 27
The UMass/Amherst Flow Cytometry Facility: Contact Eric Martz, Dept
of
Microbiology, ema...@microbio.umass.edu; is within the UMass/Amherst
Core Instrument ...
www.bio.umass.edu/mcbfacs/flowhome.html - 4k - Cached - Similar pages
- Note this

Catalog of Free Flow Cytometry Software (UMass/Amherst) - 3 visits -
Nov 29
CYTOPC: Processing softwa

re for flow cytometric data. ... [MFI can produce a gated ASCII file
of list mode data from an FCS list mode file.] ...
www.bio.umass.edu/mcbfacs/flowcat-old.html - 39k - Cached - Similar
pages - Note this

Cytometry Information by Purdue University - 16 visits - 9:43am
Free software for flow cytometry and Image analysis ... Contact Info:
Professor J. Paul Robinson Director, Purdue University Cytometry
Laboratories, ...
www.cyto.purdue.edu/ - 28k - Cached - Similar pages - Note this

A numerical recipe for accurate image reconstruction from
discrete ...
- 2 visits - Nov 28
J. Paul Robinson, Bindley Bioscience Center, Purdue University, 1203
West State St., West Lafayette, IN 47907, USA and Cytometry
Laboratories, ...
portal.acm.org/citation.cfm?id=1221224 - Similar pages - Note this

The Science Advisory Board - Protocols, Product Reviews, Member ... -
20 visits - Nov 29**************
Dr. J. Paul Robinson, Purdue University School of Veterinary
Medicine, ... But flow cytometer software allows you to select the
number of cells to analyze ...
www.scienceboard.net/community/perspectives.169.html - 30k

ISAC Homepage - ISAC E-News -- June, 2007 - 8 visits - Nov
30*************
Best wishes. J. Paul Robinson, President ..... ISAC in India was
introduced during the 7th Indian-US Flow cytometry workshop held in
New Delhi and hosted by ...
www.isac-net.org/content/view/593/119/ - 53k - Cached - Similar pages
- Note this

WORKING LINK TO FIND OUT ABOUT ISAC.....*************************
http://www3.interscience.wiley.com/aboutus/

Africa Business Conference 2007
J. Paul Robinson Director, Purdue University Cytometry Laboratories
and Deputy ... in bioengineering with hardware and software groups
developing innovative ...
www.hbsafricaconference.org/2007/panels_healthcare.html - 27k -
Cached
- Similar pages - Note this

PDF]**********************************
Purdue University Cytometry Laboratories Introduction to WinMDI ... -
5 visits - Nov 27
File Format: PDF/Adobe Acrobat - View as HTML
J. Paul ROBINSON, Ph.D. Purdue University Cytometry
Laboratories .....
The Windows Multiple Document Interface software (WinMDI) is
freeware ...
www.corelabs.emory.edu/fcm/extra/TutorialWinMDI.pdf - Similar pages -
Note this

Purdue and S. Korean Researchers Collaborate On Nanomedicine ...
J. Paul Robinson, professor in the schools of Biomedical Engineering
and Veterinary Medicine and director of Purdue's Cytometry
Laboratories at the Bindley ...
nanotechwire.com/news.asp?nid=4846&ntid=&pg=31 - 44k -

Worcester Telegram & Gazette News***************************
J. Paul Robinson, PURDUE CYTOMETRY LABORATORIES ... PointCare's
software then crunches data on light scattered by the sample to come
up with the number and ...
www.telegram.com/article/20070619/NEWS/706190363&SearchID=73285224641190
- 35k - Cached - Similar pages - Note this

Flow cytometry********************
Kina, and J. Paul Robinson, Purdue University Cytometry Laboratories
and ... and software systems in the measurement of DNA aneuploidy ...
doi.wiley.com/10.1002/cyto.990140907 - Similar pages - Note this
***************************************************************************
****************************************************
UT, MD Anderson, Science Park - Research Division Flow Cytometry ...
University of Texas, MD Anderson Science Park Flow Cytometry
References. ... Robinson, J. Paul, Ed.: Handbook of Flow Cytometry
Methods 1993, Wiley-Liss, ...
sciencepark.mdanderson.org/fcores/flow/files/references.html - 40k -
Cached - Similar pages - Note this

News: Stanford Team Launches New Approach to HIV/AIDS
Education ...*******************************
CYTOMETRY SOFTWARE**PURDUE***MARKETING**GRANTS FOR FLOW JO**J PAUL
ROBINSON** WEBSITE MARKETING **KANECKI ASSOCIATES INC BANNED? ...
www.expertcleanse.com/news-stanford-team-launches-new-approach-to-hiv...
- 15 hours ago - Similar pages - Note this

***************************************************************************
****************************************************
The Daily Dongle
Cytometry Developers Workshop is attended by the industry and
academia
alike. ... J Paul Robinson delivered an effective summary of the
problem, ...
flowjo.typepad.com/ - 40k - Cached - Similar pages - Note this ...

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Date: Sat, 1 Dec 2007 10:48:42 -0800 (PST)
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Subject: Re: CYTOMETRY SOFTWARE**PURDUE MAIL LIST***they will find
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how much influence we actuthey will find out how much influence we
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> ***************************************ONLY 3000 PEOPLE ON PURDUE
> CYTOMETRY MAIL LIST******************
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> ROBINSON** WEBSITE MARKETING **KANECKI ASSOCIATES INC BANNED? ...www.expertcleanse.com/news-stanford-team-launches-new-approach-to-hiv...
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Date: Sat, 1 Dec 2007 12:04:55 -0800 (PST)
Local: Sat, Dec 1 2007 2:04 pm
Subject: Re: CYTOMETRY SOFTWARE**PURDUE MAIL LIST***they will find
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how much influence we actuthey will find out how much influence we
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> On Dec 1, 1:33 pm, Mitch Haynes <mitchhay...@gmail.com> wrote:
> > ***************************************ONLY 3000 PEOPLE ON PURDUE
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> > Cached - Similar pages - Note this
> > News: Stanford Team Launches New Approach to

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From: Mitch Haynes <mitchhay...@gmail.com>
Date: Sat, 1 Dec 2007 12:47:08 -0800 (PST)
Local: Sat, Dec 1 2007 2:47 pm
Subject: Re: CYTOMETRY SOFTWARE**PURDUE MAIL LIST***they will find
out
how much influence we actuthey will find out how much influence we
actually have.....********While they claim to be legitimate*****I
already told them that they were scammers****
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> On Dec 1, 1:33 pm, Mitch Haynes <mitchhay...@gmail.com> wrote:
> > ***************************************ONLY 3000 PEOPLE ON PURDUE
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> > sciencepark.mdanderson.org/fcores/flow/files/references.html - 40k -
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From: Mitch Haynes <mitchhay...@gmail.com>
Date: Sat, 1 Dec 2007 12:47:48 -0800 (PST)
Local: Sat, Dec 1 2007 2:47 pm
Subject: Re: CYTOMETRY SOFTWARE**PURDUE MAIL LIST***they will find
out
how much influence we actuthey will find out how much influence we
actually have.....********While they claim to be legitimate*****I
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> > > *******************************************************************************************************************************
> > > UT, MD Anderson, Science Park - Research

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Date: Sat, 1 Dec 2007 12:54:55 -0800 (PST)
Local: Sat, Dec 1 2007 2:54 pm
Subject: Re: CYTOMETRY SOFTWARE**PURDUE MAIL LIST***they will find
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actually have.....********While they claim to be legitimate*****I
already told them that they were scammers****
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> ***************************************ONLY 3000 PEOPLE ON PURDUE
> CYTOMETRY MAIL LIST*****************

While they claim to be legitimate, they are

> acting
> exactly as any scam artist...I already told them that they were
> acting
> as scammers and they got upset with me...well, if they don't desist,
> they will find out how much influence we actually have

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> CYTOMETRY

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> Co-Chairs: Bartek Rajwa and J Paul Robinson. The Cytometry of Flow Jo ISAC 2008 New Flow Cytomety Mail List Rules and Sales Regulations NOT IN THE NEWS BECAUSE THEY DO NOT WANT YOU TO KNOW Options

I can't pass up the opportunity to get into the PC/Mac battle - I
hate
them
both, but there aren't really alternatives (yes, I know, there's
Linux, but
it's probably even harder to hook up a Linux system to hardware than
it is
to hook a Mac up to hardware).

Of course, what started this thread was the assertion in the piece
about
Mario on Apple's web site that there were 15,000 to 20,000 Mac-based
flow
cytometers out there. The best estimates I had from industry people
in
early 2003 when I was finishing up the 4th Edition of PFC was that
the
total number of systems from all manufacturers in use was under
20,000. If
that's correct, BD would need a 75% market share to account for the
low end
estimate of 15,000 Mac-based machines. I'd be interested to know
where
the
15,000 to 20,000 figure came from.

The old (68000 series and possibly early PowerPC) Macs were
difficult,
but
not impossible to connect to hardware; it was easier to work with the
PC's
ISA bus, which, while slow, was perfectly adequate to do most flow
cytometric data acquisition and analysis. There were decent versions
of
Forth, which was one of the first and best languages designed for
controlling hardware from mini- and microcomputers, for both PC (DOS)
and
the Mac (Forth was the first Mac programming language made
commercially
available, at a time when the only other option was buying the very
expensive Lisa from Apple on which to develop Mac software). I found
MacForth easier to program with than the Windows Forths (or other
Windows
languages, e.g., Delphi), but my old Macs used to crash all the time.

So what makes me unhappy with both Windows [and Windows machines] and
the
Mac in their current incarnations? It is now significantly harder for
mere
mortals to write software to get hardware to communicate with either
PCs or
Macs; the gain in complexity associated with the PCI bus, USB/USB2,
FireWire(IEEE 1394), etc. is greater than the gain in speed and
convenience. Also, in making the operating systems more stable (and
Win XP,
despite its security issues, is almost as stable as Mac's Unix-based
OS X),
both Microsoft and Apple elected to eliminate the ability of their
computer
hardware to respond rapidly to interrupts (latencies are now in the
tens of
thousands of instruction cycles), meaning that any really fast
hardware
attachment for either a PC or a Mac now needs to have a DSP in it,
whereas
if the fast interrupt response had been preserved, the hardware
attachments
could have been made much simpler and cheaper. Linux also takes fast
interrupt response off the table, so it doesn't represent a viable
alternative. If you go to Apple's web site and look at what data
acquisition hardware is available for Macs, particularly for Macs
running
OS X, there isn't much, and many of the companies that supported the
Mac in
that area have dropped Mac support for their newer products. That is
undoubtedly one big reason why BD's newer digital pulse processing
cytometers are running on the PC platform.

That doesn't stop anybody from analyzing FCS files on Macs. FlowJo is
well-conceived flow cytometry software; one reason it is as good as
it
is
is that it was written by people who do a lot of flow cytometry, and
cutting edge flow cytometry at that. But there are other people who
do
a
lot of good flow cytometry who have written good software, for PCs as
well
as for Macs.

For the record, I have a G4 PowerBook, which I use mostly for the
iLife
applications, which are slick. It doesn't crash more than once every
couple
of months, but the same is true of my Windows 2000 and XP systems. OS
X can
be as infuriating as Windows when one or another aspect of it goes
counter
to your intuition or to what you have gotten used to. Macs, while
somewhat
more expensive, are much better made than many PCs, and they are
certainly
aesthetically pleasing. If there were a reasonable alternative to
Microsoft's Office applications for the Mac, I might consider
switching.
The 12.1" PowerBook is a nice portable, but it's over a pound heavier
than
the Fujitsu laptop I now use, which has pretty much the same speed,
memory,
and drive capabilities (OK, not DVD-R, but I don't burn a lot of
DVDs).

However, I really wish Apple had stuck with the plan they had a few
years
back of writing a Mac operating system for Intel hardware. I think
that
died when Microsoft bailed Apple out with a few hundred mil. If it
were
possible to run OS X and XP mano a mano on the same hardware, there'd
be a
more rational basis for comparison. But, as may be the case for the
November election, minds, once made up, are not easily changed.

-Howard
Received on Thu Aug 26 16:38:00 2004
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RE: mr on Apple web site
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From: Fischer, Randy (NIH/NIAMS) <fisch...@mail.nih.gov>
Date: Mon Aug 23 2004 - 16:18:55 EST
Paul,

I know that for a long time, anyone who used a BD flow cytometer had
little
choice but to use a MAC-data acquisition required it. And, one of
the
best
third party software packages was FlowJo which only ran on a MAC.
Mario,
being a FlowJock, naturally was heavily exposed to this atmosphere.
However, most of our other laboratory instrumentation appears to run
on the
IBM clone type of PC-ELISA reader, luminometer, ELISpot reader, and
AFFYMETRIX machine to name the first ones that come to mind. Of
course,
because Flow is such a key component and FlowJo works so well, we
have
MACs
(and associated cinema displays) at all of our desks. So, Mario is
probably
pretty close as I guess we now run about 50% MAC, and for most things
they
do run better than our PCs for similar applications, but mostly the
two
platforms tend to be used for very disparate applications and we need
both.

Wish more science companies would move to MAC, but as Microsoft
probably
owns a minority share in most companies, that will not likely happen
in our
lifetimes.

Haven't read the article yet, but hopefully Mario remembered to cite
you for
all the work on the Flow website/mailing list.

Randy T. Fischer
NIH/NIAMS
Building 10, Room 6D50
9000 Rockville Pike
Bethesda, MD 20892
(301) 594-3537
fisch...@mail.nih.gov

- Hide quoted text -
- Show quoted text -

> ----------
> From: J. Paul Robinson
> Reply To: j...@flowcyt.cyto.purdue.edu
> Sent: Friday, August 20, 2004 2:25 PM
> To: Cytometry Mailing List
> Subject: Re: mr on Apple web site

> ummm....Mario says..
> "In life sciences - particularly in research life sciences -
[quoted text clipped - 3 lines]
> have put on my helmet and armor....waiting...)
> paul

> > For all of the mr groupies out there in cytometry cyberspace. Don't wet
> your
> > pocket protectors over this.

> > The information in this e-mail and any of its attachments is
> confidential
[quoted text clipped - 10 lines]
> > and not expressly made on behalf of the NIAID by one of its
> representatives.

> Have you seen our new HCS webpage?
> http://www.cyto.purdue.edu/hcs

> Paul:

> I use Macs exclusively. Between home and labs, I have 7.
> I hate Windows (I tell everyone, I don't do Windows).
[quoted text clipped - 5 lines]
> flat-panel display for my flow.
> Beverly

> Beverly E. Barton, Ph.D.
> Assistant Professor
> Department of Surgery/Division of Urology
> UMDNJ-NJMS MSB G519
> 185 S. Orange Avenue
> Newark, New Jersey 07103

> Telephone 973-972-0662
> E-mail barto...@umdnj.edu
> Telefacsimile 973-972-3892

> On Aug 20, 2004, at 2:25 PM, J. Paul Robinson wrote:

> > ummm....Mario says..
> > "In life sciences -- particularly in research life sciences --
[quoted text clipped - 3 lines]
> > have put on my helmet and armor....waiting...)
> > paul

> >> For all of the mr groupies out there in cytometry cyberspace. Don't
> >> wet your
> >> pocket protectors over this.

> > Have you seen our new HCS webpage?
> > http://www.cyto.purdue.edu/hcs